威海地区高压岩石组合及其成因演化

利用矿物地质温压计估算变质高峰期温压条件、退变质作用温压条件。研究表明,本区为鲁苏构造带的北延部分,代表本区晚元古代华南陆块与华北陆块的碰撞及三叠纪华北陆块向华南陆块推覆事件的发生和演化。     

Molecular analysis of complete ssu to lsu rdna sequence in the harmful dinoflagellatealexandrium tamarense (korean isolate, HY970328M)

New PCR primers (N=18) were designed for the isolation of complete SSU to LSU rDNA sequences from the dinoflagellateAlexandrium tamarense. Standard PCR, employing each primer set selected for amplifications of less than 1.5 kb, successfully amplified the expected rDNA regions of A. tamarense (Korean isolate, HY970328M). Complete SSU, LSU rDNAs and ITS sequences, including 5.8S rDNA, were recorded at 1,800 bp, 520 bp and 3,393 bp, respectively. The LSU rDNA sequence was the first report inAlexandrium genus. No intron was found in the LSU rRNA coding region. Twelve D-domains within the LSU rDNA were put together into 1,879 bp (44.4% G+C), and cores into 1514 bp (42.8% G+C). The core sequence was significantly different (0.0867 of genetic distance, 91% sequence similarity) in comparison withProrocentrum micans (GenBank access. no. X16108). The D2 region was the longest in length (300 bp) and highly variable among the 12 D-domains. In a phylogenetic analysis using complete LSU rDNA sequences of a variety of phytoplankton,A tamarense was clearly separated with high resolution against other species. The result suggests that the sequence may resolve the taxonomic ambiguities ofAlexandrium genus, particularly of the tamarensis complex.     

耳鲍(Haliotis asinina)核糖体小亚基(18S rRNA)编码基因的克隆与序列分析

采用分子生物学的方法,对南海不同海区的两个地理群体耳鲍(Haliotis asinina)18S rRNA基因全长进行了克隆和序列分析,并将耳鲍18S rRNA基因的序列与NCBI数据库中已收录鲍的18SrRNA基因进行了比较。结果发现,南海耳鲍核糖体18S rRNA基因与耳鲍H.asinina isolate H11核糖体18S rRNA基因序列的相同率高达98%;同一地理群体内耳鲍核糖体18S rRNA基因序列完全一致;不同地理群体间耳鲍核糖体18S rRNA基因在碱基组成上的相似率为99%,仅在某些位点处发生了碱基替换,即腺嘌呤(T)被鸟嘌呤(G)替换;同时,将这两个不同群体中耳鲍的18S rRNA基因与泰国耳鲍18S rRNA基因序列进行比较分析发现,它们之间也只是发生了碱基替换。     

深圳大鹏湾表层沉积物碎屑矿物空间分布特征

立足样品测试数据,利用空间分析、空间插值、地质统计以及景观生态学方法,深入分析了深圳大鹏湾表层沉积物碎屑矿物的空间分布特征,并使用球状模型对相应半变异函数进行理论建模。结果表明,碎屑矿物分布的Hurst分维数为0.28～0.97,空间几何分维数为1.03～1.72;Moran Ⅰ型空间自相关系数-0.15～0.53。在此基础上,以等值线分布所包围范围作为划分尺度,以Shannon-Wiener信息指数作为度量,得到的矿物分布所反映出的信息量或者富集程度指数为0.88～1.85。     

棉粕蛋白酶解物对异育银鲫(Carassius auratus gibelio)消化、生长和胰蛋白酶mRNA表达量的影响

以棉粕蛋白为酶解底物,用枯草杆菌蛋白酶对其进行酶解,以酶解产物1.5%和3.0%两个梯度等量替代鱼饲料配方中棉粕,在室内流水养殖系统中喂养异育银鲫鱼种[体重为(30±2)g]65天。测定鱼的生长、营养物质表观消化率、消化蛋白酶活性及肝胰脏中胰蛋白酶mRNA表达水平等指标。结果表明,添加1.5%和3.0%棉粕酶解产物的鱼在饲养35天后的特定增长率(SGR)分别比对照组高32.5%和56.7%,且差异显著(P<0.05);在饲养65天后,两组特定增长率分别比对照组高8.0%和21.0%,且差异极显著(P<0.01),肝胰脏中胰蛋白酶mRNA表达水平也随棉粕酶解产物添加梯度提高而相应提高,表明鱼的生长与消化蛋白酶mRNA表达水平相关。同时棉粕蛋白酶解物对肠道蛋白酶的活性和营养物质表观消化率都有促进作用,而棉粕蛋白酶解物对鱼肌肉成分并没有改变,这也表明棉粕蛋白酶解物在促进鱼生长、内源酶活性同时并未降低鱼的品质。     

天然热释光技术在海洋油气田勘查中的应用

沉积物热释光能有效地反映海洋环境下深部油气田引起的微弱放射性异常。热释光在不同的测试粒级、不同的岩性中的丰度不同，结果为粗粒级＞细粒级、砂岩＞粉砂质砂岩＞泥质粉砂岩＞泥岩。热释光也与部分金属元素含量有关，Ti、Ba、Zn、Fe^3 /Fe^2 与热释光呈正相关关系。热释光与烃类也呈较强的正相关性。不同采用深度热释光测量结果表明，热释光剂量随沉积物的沉积深度和沉积时间的增加而增加，深层样的热释光对油气藏的指示效果更好。     

中国鳚亚目鱼类地理分布和区系特征

鳚亚目鱼类是鲈形目中较大的一个亚目，现知全世界有127属，700余种(Nelson，1994)。目前还不断有新种发现，多数为海洋鱼类，世界性分布。 鳚亚目鱼类大多体型较小，对它们的研究也少。随着科学技术的发展和研究方法的不断完善，人们对这一类群的认识也愈来愈深入，其种类、区系与动物地理学等方面的特点正逐渐为研究者所揭示。其中有些种类由于在海洋生态学、环境生物学和渔业生产上具有重要意义而吸引着许多鱼类学家和生态学家的注意和对其进行研究(Kotrschal，1992；Springer，1993；Stepien，1993)。 印度-西太平洋是鳚亚目鱼类种类最多的海区之一，中国及周边国家和地区在此领域已有一定的研究基础。日本学者Masuda等(1984)总结了日本沿岸鳚亚目鱼类有86属212种，菲律宾已知有36属80余种(Herre，1953)。中国是世界上海岸线最长的国家之一，适合于鳚亚目鱼类栖息的生境相当广泛，种类也相当丰富。作者根据目前已掌握的标本和文献资料，经初步分析得出中国海区现有鳚亚目鱼类33属78种。     

利用GRACE时变重力场监测中国及周边地区的水储量变化

利用GRACE时变重力场反演了2005年1～11月的中国及周边地区的水储量月变化,并对结果进行了分析。研究表明,利用GRACE时变重力场可以很好的对水储量进行监测,其监测能力足以揭示几个厘米的等效水高,为全球水循环及其气候变化提供了可靠的资料。     

东海大规模赤潮危害影响的实验研究——对黑褐新糠虾和卤虫的毒性效应

The effects of Prorocentrum donghaiense,Karenia mikimotoi,Alexandrium catenella,the causative species of Harmful Algal Blooms in the East China Sea,on the survival and growth of Neomysis awatschensis and Artemia salina were investigated.And the toxicity of eleven strains of Alexandrium----A.lusitanicum(AL-1)、A.minutum(AM-1)、A.spp.(A-K)、A.tamarense(ATHK,ATCI02,ATCI03,AT5-1,AT5-3,AT-6)、A.affine(AS-1,AC-1) to the two above organisms were compared. The results showed that P.donghaiense and K.mikimotoi had no adverse effect on the survival and growth of N.awatschensis and A.salina at 96h.However,the survival rate of N.awatschensis and A.salina declined linearly with the increasing density of A.catenella,and the 96h LC₅₀ for N.awatschensis and A.salina was 4500 and 460 cells/mL,respectively, Five strains of Alexandrium----A.spp,A.lusitanicum (AL-1),A.tamarense(AT5-3,AT-6) and A.affine (AS-1)at the density of 4000cells/mL and 96h had no adverse impacts on N.awatschensis. But other six strains of Alexandrium a.Tamarense(AT5-1,ATHK,ATCI02,ATCI03),A.affine(AC-1) and A.minutum(AM-1)decreased the survival fate of N.awatschensis significantly. Two strains of Alexandrium----A.lusitanicum(AL-1) and A.tamarense(AT-6),at the density of 500cells/mL. had no adverse effects on A.salina in 96h;while the other Alexandrium strains all had adverse effeets on the survival of a.salina, A.salina could increase its length with the increasing density of P.donghaiense,K.mikimotoi and A.luxitanicum. The results indicate that P.donghaiense and K.mikimotoi have nio significant effect on the survival of crustaeean zoop lankton in a short period. But several Alexandrium specised/strains could influence the survival of crustacean zooplankton likely by non-PSP substances. A.salina is shown to be more sensitive than N.awatschensis as test organisms to harmful species.     

Changes in the hydrography of Central California waters associated with the 1997–98 El Niño

Oceanographic conditions off Central California were monitored by means of a series of 13 hydrographic cruises between February 1997 and January 1999, which measured water properties along an oceanographic section perpendicular to the California Coast. The 1997–98 El Niño event was defined by higher than normal sea levels at Monterey, which began in June 1997, peaked in November 1997, and returned to normal in March 1998. The warming took place in two distinct periods. During June and July 1997, the sea level increased as a result of stronger than normal coastal warming below 200 dbars and within 100 km of the coast, which was associated with poleward flow of saltier waters. During this period, deeper (400–1000 dbar) waters between 150–200 km from shore were also warmed and became more saline. Subsequently, sea level continued to rise through January 1998, mostly as a result of the warming above 200 dbars although, after a brief period of cooling in September 1997, waters below 200 dbar were also warmer than normal during this period. This winter warm anomaly was also coastally trapped, extending 200 km from shore and was accompanied by cooler and fresher water in the offshore California current. In March and April 1998, sea level dropped quickly to normal levels and inshore waters were fresher and warmer than the previous spring and flowed southward.The warming was consistent with equatorial forcing of Central California waters via propagation of Kelvin or coastally-trapped waves. The observed change in heat content associated with the 1997–98 El Niño was the same as that observed during the previous seasonal cycle. The warming and freshening events were similar to events observed during the 1957–58 and 1982–83 El Niños.