The effect of copper pollution on mitochondrial degeneration

The authors describe the effect of copper pollution on the mitochondria from various areas of the Torpedo marmorata central nervous system. A high percentage of swollen mitochondria has been observed in neurons from animals experimentally exposed to high level of copper (4 ppm). The correlation with age pigment granules is discussed.     

Nitrate assimilation by zooxanthellae maintained in laboratory culture

The ability of cultured zooxanthellae to use ammonium and nitrate was tested. The zooxanthellae were initially isolated from the anemone Aiptasia pulchella. The zooxanthellae were able to grow on media supplemented with either form of dissolved inorganic nitrogen (DIN) although ammonium was inhibitory above 300 μM. In accord with the intact symbiosis and freshly isolated algae, the cultured zooxanthellae took up ammonium. In contrast, the cultured algae demonstrated nitrate uptake and utilization. This was enhanced with DIN deprivation. Nitrate reductase was found in the algae and appeared to be inducible. Within the animal host, excretory ammonium may repress this induction.     

Effects of pulp bleach plant effluents on hepatic xenobiotic biotransformation enzymes in fish: Laboratory and field studies

The present preliminary report on studies of the influence of pulp bleach plant effluents on hepatic biotransformation enzymes in fish is a part of a major characterisation of biochemical and physiological effects of effluents from pulp industries in Sweden. Our studies on the xenobiotic biotransformation enzymes demonstrate, so far, that the pulp effluents have the potential to strongly induce 7-ethoxyresorufin-O-deethylase activity (EROD) in fish liver and that fish living in the receiving water of an effluent from a pulp bleach plant have induced hepatic EROD activities as well as higher hepatic UDP glucuronyltransferase activities than those living in an unpolluted area. It is thus apparent that measures of induction of biotransformation enzymes in fish liver may be a useful approach in monitoring the presence of potential hazardous compounds released from pulp industries.     

Evaluation of drift current parameters using an analytical model

The paper is concerned with the evaluation of the drift current parameters derived through the use of an analytical model. In this model, effective when stratification is stable and indifferent, the vertical turbulence coefficient profile is prescribed by the power function, and hydrodynamic quantities are prescribed using the external parameters of the problem (wind stress, the Coriolis parameter, and the dimensionless stratification parameter). Model data are compared with the observations of the upper mixed layer in the vicinity of the oceanic Station C, conducted during one year. It is shown that, under the spring-summer-time warming conditions, the model at issue is capable of adequately simulating the upper ocean layer dynamics. Translated by Vladimir A. Puchkin.     

Wave loading on axisymmetric bodies using axisymmetric hybrid integral equation method

The wave diffraction problem on axisymmetric structures are solved by treating the fluid field as two separate domains. The velocity potential in the inner domain is represented by a 1/r type Green's function whilst that of the outer domain is represented by an eigenfunction expansion. The simple form of the Green's function in the inner domain reduces significantly the computational effort whilst the eigenfunction expansion in the outer domain is able to satisfy the radiation boundary condition completely. The method requires to have elements cover the entire containing boundary. Results for a number of typical structural geometries are presented and discussions are made on the effect of various parameters.     

Advances in the fatigue assessment of wire ropes

This paper presents the findings from an in-depth analysis of the (axial) stiffness data recorded during tension–tension fatigue tests on wire ropes, particularly in relation to how changes in stiffness during testing relate to changes in rope strength. A linear relationship between stiffness and strength is shown to exist and a methodology presented for quantifying residual strength with applied cycles. New lower bound fatigue lines for six-strand rope and spiral strand are presented which are based on a 10% loss of strength. These new lines have the advantage of having been established using a common discard criterion for wire ropes.     

White-light promoted degradation of leucopterin and related pteridines dissolved in seawater, with evidence for involvement of complexation from major divalent cations of seawater

The photochemical instability of several related pteridines in seawater was investigated by aseptic incubation of solutions at 20–22°C under illumination from cool-white light of intensity 6 kerg cm⁻² sec⁻¹, and the chemical changes were spectrophotometrically monitored. All the pteridines showed markedly accelerated degradation from this illumination relative to their behaviour in total darkness.Pterin and lumazine were degraded very slowly with zero-order reaction kinetics, while the other pteridines photolysed rapidly (according to first-order kinetics) with decomposition rates increasing in the order dioxylumazine (2,4,6,7-tetrahydroxypteridine) < leucopterin < isoxanthopterin < xanthopterin < oxylumazine (2,4,6-trihydroxypteridine). Excepting leucopterin and dioxylumazine, the photolysis rates were attributable to the pH of seawater and not its salt content; this was also the case with oxylumazine which required the salt content of seawater for decomposition in darkness. Leucopterin and dioxylumazine (both 6,7-dihydroxylated pteridines) gave evidence of complexation with the major divalent cations (Ca²⁺, Mg²⁺) of seawater, by virtue of which their photolytic degradation rates were enhanced to magnitudes obtained in pH-10 buffer without seawater. It is proposed that such complexation produces structural forms of these pteridines analogous to their normal ionic forms at pH 10–12.The photolysis of the 6-hydroxylated pteridines (xanthopterin, oxylumazine) proceeded via intermediate formation of their corresponding 7-hydroxylated derivatives (leucopterin, dioxylumazine).     

Uptake and transformation of C-labelled tributyltin chloride by the dog-whelk, nucella lapillus: Importance of absorption from the diet

The importance of the diet as a source of tributyltin (TBT) in Nucella lapillus was studied using [¹⁴C]tributyltin chloride. In N. lapillus provided with prelabelled mussels, Mytilus edulis, in labelled water (mean 20·5 ng/litre TBT) the rate of accumulation of total ¹⁴C was 2–3 times that in unfed animals. Owing to its degradation in the tissues of both fed and unfed animals, concentrations of [¹⁴C]TBT tended to reach a plateau after only 28 days. However, total concentrations of ¹⁴C were still increasing after 49 days. Under experimental conditions (15°C, ample food, no disturbance) the diet accounted for about 50% of the body burden of TBT in N. lapillus after 49 days exposure: concentration factors (dry tissue/water) for [¹⁴C]TBT in both male and female N. lapillus were similar at about 60 000 in fed and 30 000 in unfed animals. It is concluded that the diet may contribute less than half of the body burden of TBT found in natural populations subjected to life-long exposure.