火山玻璃风化层的透射电镜研究

利用透射电镜(TEM)和X射线能谱(EDX)研究了长白山天池火山1000年和4000年前两次大喷发产生的火山玻璃风化层特征.TEM分析显示,4000年前大喷发浮岩中的火山玻璃风化层平均厚度3.7 mm,1000年前大喷发浮岩中的火山玻璃风化层平均厚度为1.0 mm.EDX分析显示,两次大喷发浮岩中的火山玻璃风化层化学组成与火山玻璃相比富Al、Fe,而si减少.火山玻璃风化层富Al发生在Al的浓度较高的中到弱酸溶液中(pH=5～6),火山玻璃表面形成含有少量的非晶质的Al、Si、Fe物质,这些非品质的次生物质是火山玻璃风化早期阶段形成的.天池火山喷发物中火山玻璃的微观特征的差异可能与火山喷发年代和喷发后的环境有关,研究天池火山不同期次喷发物中火山玻璃的微观特征具有一定的理论和实际应用价值.     

温度对不同大小墨西哥湾扇贝生长的影响

在8—32℃实验室控温范围内以2℃为梯度,以壳高及体重增长作为观察指标,研究3个规格墨西哥湾扇贝Argopecten irradians concentricus的适宜、较适宜及最适宜生长温度。研究结果表明,小苗(壳高6.2mm,体重48.3mg)、中苗(壳高18.1mm,体重1191.1mg)、大苗(壳高30.7mm,体重5809.9mg)在8—32℃水温下均能生长,但在水温8及32℃,3种规格增长率均较低;适宜生长温度分别为小苗11.2—31.1℃、中苗9.8—31.8℃、大苗9.8—32.4℃;较适宜生长温度分别为小苗18.1—29.4℃、中苗18.5—29.4℃、大苗16.9—30.4℃;3种苗随规格增大,适温范围扩大(p<0.01);最适生长温度3种苗均为24—28.0℃,其范围向高温端漂移;在水温为8℃时,3种苗存活率均达到97.0%以上,水温在32℃时则小苗为47.0%、中苗为67.5%、大苗为76.0%,表现出3种苗对低温有更强的忍耐力,大苗对高温有更强的适应性。     

河口环流和盐水入侵Ⅱ--径流量和海平面上升的影响

本文利用本序列上篇论文建立的理想河口数值模式,研究径流量和海平面上升对河口环流和盐水入侵的影响.在径流量增大的情况下,口门内表层向海的流速增大,底层向陆的密度流减弱,滞流点下移.口门外侧向口门的密度流增大,上升流趋于增强.口门内盐水入侵减弱,口外盐度减小、冲淡水扩展范围增大.在口门上游北岸底层盐度下降明显,口门处南岸表层盐度下降明显.径流量变化对盐水入侵影响十分巨大.在海平面上升的情况下,拦门沙区域向陆的密度流增强,滞流点上移,表层向海的流动增大.口门内盐水入侵增强,口外盐度增大,冲淡水扩展范围减小.海平面上升对盐水入侵影响十分明显,北岸底层盐度增大尤为特出.     

中国对虾染色体制备及染色体形态的研究

以中国对虾原肠期胚胎为材料，采用整体低渗法和管渗法均制备了染色体。通过比较发现，管渗法制备的染色体比整体低渗法的形态好。本文对制备中国对虾染色体技术方面的问题进行了探讨。通过作者的观察，了解到中国对虾染色体徐正常特征外，还存在较特别的形态特征，如主缢痕增强，次缢痕变化，同源配对等。并对产生这些形态特征的原因进行了解释。     

河口Si输运过程研究

基于拉格朗日余流及其输运过程的一种三维空间弱非线性理论，进一步假定Ｓｉ在河口的无因次化学转移项量级为ｋ＾２，给出了Ｓｉ的长期输运方程。对于一种二维模型河口，数值求解了零阶天文潮，欧拉余流、斯托克斯漂移、拉格朗日余流和盐度的分布；分别计算了平均逼留时间为１３，３０，６０，１８０ｄ等所对应的Ｓｉ浓度分布；给出了Ｓｉ－ｓ相关图，讨论了河口中Ｓｉ的保守性问题。     

青海湖全新世植被演变及气候变迁：QH85—14C孔孢粉数值分析

本文应用青海湖QH85-14C孔取得的具有~(14)C测年数据所支持的原始孢粉资料,进行数值分析(有序聚类、主成份分析、滑动平均和回归分析)。从而对青海湖区11000年以来的植被和气候变化进一步讨论。     

对虾一种无包涵体杆状病毒病原的PCR检测

为建立对虾无包涵体杆状病毒的快速诊断技术,应用PCR技术分别对暴发性流行病发生前、流行中和发病后的对虾样品进行了检测.根据已克隆的对虾杆状病毒部分基因组序列而设计的引物能特异性地扩增出靶DNA片段,最低可以检测1pg的病素DNA.由典型发病症状对虾的胃、腮、肝胰腺、中肠、游泳足、肌内和心脏等器官和组织中成功地检测到病毒.不同组织和器官经扩增得到的信号强弱不同:病虾的胃扩增得到的信号最强,中肠、肌肉、心脏和游泳足次之,腮和肝胰腺信号最弱,说明病毒在不同组织和器官中数量及感染程度不同.在对虾发病前及发病后,用二次PCR还能检测表面不发病呈隐性感染状态的对虾携带的病毒;而对野生健康虾的检测结果为阴性.研究表明,PCR是检测对虾暴发性流行病快速、灵敏而准确的方法,对病毒病的早期诊断、防治和高健康对虾品种的选育具有指导意义.     

Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.     

Variation in Water Masses on Shelf of Northern Bering Sea in September 2016-2018

Abstract

Based on hydrological data obtained during the 7th to 9th Chinese National Arctic Research Expeditions in the summers of 2016–2018, the main water structure on the shelf of the northern Bering Sea and the volume and heat fluxes of the Bering Strait throughflow were analyzed. Distinct variability was identified in the three Pacific water masses feeding the strait - Anadyr Water (AW), Bering Shelf Water (BSW) and Alaskan coastal water (ACW). Overall, the temperature and salinity of the entire section increased each year, with 2018 showing significant anomalies, i.e., a temperature anomaly of up to 1?°C and a maximum salinity anomaly of 2. From 2016 to 2018, the extent of the ACW gradually narrowed in the eastern part of this section, while the AW expanded eastward each year. The net volume transport through each of the three sections increased poleward from 1.65?Sv to 2.76?Sv, with the AW increasing from 0?Sv to 1.03?Sv, the BSW varying between 0.52–1.65?Sv, and the ACW gradually decreasing from 1.04?Sv to disappearing completely. The net heat fluxes were also poleward, varying between 25.77 TW and 61.50 TW, and showing a significant increase. Significant variations in magnitude and extent were observed in each water mass of the Bering Strait throughflow, which could produce widespread effects in the Arctic Ocean and the global ocean beyond.     

褐牙鲆(♀)、夏鲆(♂)及其杂交子一代线粒体16S rDNA序列遗传特性的初步研究

以线粒体16S rRNA基因部分片段为代表研究雌褐牙鲆Paralichthys olivaceus、雄夏鲆P. dentatus及其杂交子一代间线粒体DNA的遗传特性。母本与父本序列差异较大,属种间差异,在590个位点中有28个变异位点,而且全部为简约信息位点。使用同一对引物扩增亲本与杂交子代,在杂交子代中未检测到父本的线粒体DNA类型,11个处于两种生长阶段的杂交样本仅检测到一种单倍型,而且与母本的一个单倍型为同一种,表明褐牙鲆与夏鲆杂交线粒体DNA遵循母系遗传规律。