Use of Sonication for Enhanced Sampling of Attached Microbes from Groundwater Systems

The vast majority of microorganisms in aquifers live as biofilms on sediment surfaces, which presents significant challenges for sampling as only the suspended microbes will be sampled through normal pumping. The use of a down-well low frequency sonicator has been suggested as a method of detaching microbes from the biofilm and allowing rapid sampling of this community. We developed a portable, easy to use, low-frequency electric sonicator and evaluated its performance for a range of well depths (tested up to 42 m below ground level) and casing types. Three sonicators were characterized in laboratory experiments using a 1 m long tank filled with pea gravel. These included a commercially available pneumatic sonicator, a rotating flexible shaft sonicator, and the prototype electric sonicator. The electric sonicator detached between 56 and 74% of microbes grown on gravel-containing biobags at distances ranging between 2 and 50 cm from the sonicator. The field testing comprises of a total of 55 sampling events from 48 wells located in 4 regions throughout New Zealand. Pre- and post-sonication samples showed an average 33 times increase in bacterial counts. Microbial sequence data showed that the same classes are present in pre- and post-sonicated samples and only slight differences were seen in the proportions present. The sampling process was rapid and the significant increases in bacterial counts mean that microbial samples can be quickly obtained from wells, which permits more detailed analysis than previously possible.     

The influence of solar‐induced thermal stresses on the mechanical weathering of rocks in humid mid‐latitudes

The role of solar‐induced thermal stresses in the mechanical breakdown of rock in humid‐temperate climates has remained relatively unexplored. In contrast, numerous studies have demonstrated that cracks in rocks found in more arid mid‐latitude locations exhibit preferred northeast orientations that are interpreted to be a consequence of insolation‐related cracking. Here we hypothesize that similar insolation‐related mechanisms may be efficacious in humid temperate climates, possibly in conjunction with other mechanical weathering processes. To test this hypothesis, we collected rock and crack data from a total of 310 rocks at a forested field site in North Carolina (99 rocks, 266 cracks) and at forested and unforested field sites in Pennsylvania (211 rocks, 664 cracks) in the eastern United States. We find that overall, measured cracks exhibit statistically preferred strike orientations (47° ± 16), as well as dip angles (52° ± 24°), that are similar in most respects to comparable datasets from mid‐latitude deserts. There is less variance in strike orientations for larger cracks suggesting that cracks with certain orientations are preferentially propagated through time. We propose that diurnally repeating geometries of solar‐related stresses result in propagation of those cracks whose orientations are favorably oriented with respect to those stresses. We hypothesize that the result is an oriented rock heterogeneity that acts as a zone of weakness much like bedding or foliation that can, in turn, be exploited by other weathering processes. Observed crack orientations vary somewhat by location, consistent with this hypothesis given the different latitude and solar exposure of the field sites. Crack densities vary between field sites and are generally higher on north‐facing boulder‐faces and in forested sites, suggesting that moisture‐availability also plays a role in dictating cracking rates. These data provide evidence that solar‐induced thermal stresses facilitate mechanical weathering in environments where other processes are also likely at play. Copyright © 2015 John Wiley & Sons, Ltd.     

Submarine Groundwater Discharge-Derived Nutrient Loads to San Francisco Bay: Implications to Future Ecosystem Changes

Submarine groundwater discharge (SGD) was quantified at select sites in San Francisco Bay (SFB) from radium (²²³Ra and ²²⁴Ra) and radon (²²²Rn) activities measured in groundwater and surface water using simple mass balance box models. Based on these models, discharge rates in South and Central Bays were 0.3?C7.4?m³?day^?1?m^?1. Although SGD fluxes at the two regions (Central and South Bays) of SFB were of the same order of magnitude, the dissolved inorganic nitrogen (DIN) species associated with SGD were different. In the South Bay, ammonium (NH ₄ ⁺ ) concentrations in groundwater were three-fold higher than in open bay waters, and NH ₄ ⁺ was the primary DIN form discharged by SGD. At the Central Bay site, the primary DIN form in groundwater and associated discharge was nitrate (NO ₃ ^? ). The stable isotope signatures (??¹⁵N_NO3 and ??¹⁸O_NO3) of NO ₃ ^? in the South Bay groundwater and surface waters were both consistent with NO ₃ ^? derived from NH ₄ ⁺ that was isotopically enriched in ¹⁵N by NH ₄ ⁺ volatilization. Based on the calculated SGD fluxes and groundwater nutrient concentrations, nutrient fluxes associated with SGD can account for up to 16?% of DIN and 22?% of DIP in South and Central Bays. The form of DIN contributed to surface waters from SGD may impact the ratio of NO ₃ ^? to NH ₄ ⁺ available to phytoplankton with implications to bay productivity, phytoplankton species distribution, and nutrient uptake rates. This assessment of nutrient delivery via groundwater discharge in SFB may provide vital information for future bay ecological wellbeing and sensitivity to future environmental stressors.     

Cytochrome P4501A biomarker indication of the timeline of chronic exposure of Barrow's goldeneyes to residual Exxon Valdez oil

We examined hepatic EROD activity, as an indicator of CYP1A induction, in Barrow’s goldeneyes captured in areas oiled during the 1989 Exxon Valdez spill and those from nearby unoiled areas. We found that average EROD activity differed between areas during 2005, although the magnitude of the difference was reduced relative to a previous study from 1996/1997, and we found that areas did not differ by 2009. Similarly, we found that the proportion of individuals captured from oiled areas with elevated EROD activity (?2 times unoiled average) declined from 41% in winter 1996/1997 to 10% in 2005 and 15% in 2009. This work adds to a body of literature describing the timelines over which vertebrates were exposed to residual Exxon Valdez oil and indicates that, for Barrow’s goldeneyes in Prince William Sound, exposure persisted for many years with evidence of substantially reduced exposure by 2 decades after the spill.     

Differences in the Feeding Ecology of Two Seagrass-Associated Snails

Grazing by small epifauna on live seagrass leaves was formerly viewed as unimportant in controlling plant biomass and growth, instead researchers focused on the indirect benefits of small invertebrates that crop algal competitors. Recent evidence shows that the emerald nerite Smaragdia viridis preferentially ingests seagrass leaf tissue. In contrast, the button snail Modulus modulus feeds on epiphytes and periphyton coating the leaves. We conducted laboratory microcosm and field experiments to investigate how the different feeding preferences of these seagrass-associated snails affect turtlegrass Thalassia testudinum primary production. Data revealed that after 24 h S. viridis reduced foliar biomass (25%) and chlorophyll (30%) and injured the equivalent of 50% of daily seagrass growth per shoot. Conversely, M. modulus did not affect these variables. Our results emphasize that in subtropical seagrass communities not all small epifauna browse off leaf surfaces and some can have important direct negative impacts on their seagrass host.     

The relationship between reference canopy conductance and simplified hydraulic architecture

Terrestrial ecosystems are dominated by vascular plants that form a mosaic of hydraulic conduits to water movement from the soil to the atmosphere. Together with canopy leaf area, canopy stomatal conductance regulates plant water use and thereby photosynthesis and growth. Although stomatal conductance is coordinated with plant hydraulic conductance, governing relationships across species has not yet been formulated at a practical level that can be employed in large-scale models. Here, combinations of published conductance measurements obtained with several methodologies across boreal to tropical climates were used to explore relationships between canopy conductance rates and hydraulic constraints. A parsimonious hydraulic model requiring sapwood-to-leaf area ratio and canopy height generated acceptable agreement with measurements across a range of biomes (r²=0.75)$(r^2=0.75)$. The results suggest that, at long time scales, the functional convergence among ecosystems in the relationship between water-use and hydraulic architecture eclipses inter-specific variation in physiology and anatomy of the transport system. Prognostic applicability of this model requires independent knowledge of sapwood-to-leaf area. In this study, we did not find a strong relationship between sapwood-to-leaf area and physical or climatic variables that are readily determinable at coarse scales, though the results suggest that climate may have a mediating influence on the relationship between sapwood-to-leaf area and height. Within temperate forests, canopy height alone explained a large amount of the variance in reference canopy conductance (r²=0.68)$(r^2=0.68)$ and this relationship may be more immediately applicable in the terrestrial ecosystem models.     

Precambrian sulphide deposits: R.W. Hutchinson,C.D. Spence and J.M. Franklin (Editors). Geological Association of Canada,Special Paper 25, H.S. Robinson Memorial Volume 1982, VII + 791 pp., US$ 47.00 plus US$ 2.50 postage and handling for members,and US$ 57.00 plus US$ 2.50 postage and handling for others,hardcover

The Precambrian of Madagascar appears to be a part of the Mozambiquian mobile belt. Some authors consider the southern part of the island to be constituted, from east to west, by more and more recent formations, i.e., the ultrametamorphic ‘Androyan’ sequence with an Archean reworked crust as the oldest one, first followed by the ‘Graphite’ sequence, then by the ‘Vohibory’ sequence. The cordierite and garnet banded gneisses and leptynites are widely distributed in the Androyan sequence. These gneisses result from an incipient partial melting of paragneisses. Garnet—plagioclase, garnet—cordierite and garnet—biotite geothermo- and geobarometers allow the estimation of the conditions of this migmatisation (T > 700°C, P_T = 5?5.5 kbar, PH₂O = 0.3?0.4 P_T). Some cordierite and garnet leptynites represent the leucosomes of this anatectic event. Since previous experiments showed that the seven-phase assemblage (quartz + plagioclase + K feldspar + garnet + biotite + sillimanite + cordierite) only occurs in a limited range of pressure and temperature (T = 690?710°C, P_T = 4?5.7 kbar, PH₂O = 0.2?0.5 P_T), we can consider these gneisses as very precise thermobarometric reference levels in southern Madagascar. This migmatic event may be contemporary with the Pan-African orogenesis.     

An Australian View

There appears to be some confusion as to the behaviour of false colour film when used for photo-interpretation in forestry studies. The authors, working in Australia, call for a critical examination of the value of this film type. Their plea has prompted a statement, based on European experience, which forms the second section of the paper.     

AUSTRALIS: A new tool for the study of isotopic systems and geochronology in mineral systems

AUSTRALIS (AMS for Ultra Sensitive TRAce eLement and Isotopic Studies) is a microbeam accelerator mass spectrometry (AMS) system designed for in situ microanalysis of geological samples for trace elements and radiogenic and stable isotope data. The AMS method eliminates molecular and isobaric interferences in in situ mass spectrometric measurements, opening up new opportunities in geochronology and tracer applications. Tests have been carried out for measurements of Pb, S and Os isotopes, conducted mainly at 1.5 MV accelerating voltage. In Pb and S tests, precision as high as 0.3‰ has been obtained, made possible by a fast isotope switching system to counter the effect of instabilities in the ion source and beam transport system. In trace‐element analysis, a detection limit for Au at the sub‐ppb level was obtained.     

Public support for carbon dioxide removal strategies: the role of tampering with nature perceptions

Climatic Change - Carbon dioxide removal (CDR) describes a suite of controversial approaches to mitigating climate change that involve removing existing carbon dioxide from the atmosphere. Through...