中国对虾肌动球蛋白变性后ATPase活性的研究

在中国对虾肌动球蛋白（Ａｏｔｏｍｙｏｓｉｎ）经冷冻变性和热变性后，对Ｃａ２＋－ＡＴＰａｓｅ、Ｍｇ２＋－ＡＴＰａｓｅ活性及蛋白质溶解度进行了研究。冷冻变性之后，肌动球蛋白Ｃａ２＋－ＡＴＰａｓｅ活性呈逐渐下降趋势，一般贮藏温度越低，活性降低越快。热变性之后，肌动球蛋白Ｃａ２＋或Ｍｇ２＋－ＡＴＰａｓｅ活性都随温度升高而下降，但肌动球蛋白Ｃａ２＋或Ｍｇ２＋－ＡＴＰａｓｅ活性在３５℃有一急剧下降的拐点，并分别在４５℃、４０℃附近低到０。由于蛋白质的变性，其溶解度也降低     

Modulus defect,velocity dispersion and attenuation in partially-saturated reservoirs of Jurassic sandstone,Indus Basin,Pakistan

Partially saturated reservoirs are one of the major sources of seismic wave attenuation, modulus defect and velocity dispersion in real seismic data. The main attenuation and dispersion phenomenon is wave induced fluid flow due to the heterogeneity in pore fluids or porous rock. The identification of pore fluid type, saturation and distribution pattern within the pore space is of great significance as several seismic and petrophysical properties of porous rocks are largely affected by fluid type, saturation and fluid distribution pattern. Based on Gassmann-Wood and Gassmann- Hill rock physics models modulus defect, velocity dispersion and attenuation in Jurassic siliclastic partially-saturated rocks are studied. For this purpose two saturation patterns - uniform and patchy - are considered within the pore spaces in two frequency regimes i.e., lower frequency and higher frequency. The results reveal that at low enough frequency where saturation of liquid and gas is uniform, the seismic velocity and bulk modulus are lower than at higher frequency where saturation of fluid mixture is in the form of patches. The velocity dispersion and attenuation is also modeled at different levels of gas saturation. It is found that the maximum attenuation and velocity dispersion is at low gas saturation. Therefore, the dispersion and attenuation can provide a potential way to predict gas saturation and can be used as a property to differentiate low from high gas saturation.     

Multivariate approach for chlorophyll-a and suspended matter retrievals in Case II type waters using hyperspectral data

Abstract

Remote sensing has become promising in providing temporal and spatial information on biogeodynamics in large and open freshwater bodies. In optically complex environments, such as in the Western Basin of Lake Erie (WBLE), the water contains multiple biogeochemical constituents or colour producing agents (CPAs), such as phytoplankton, suspended matter and dissolved organic carbon; identifying and analysing such in-water constituents is crucial for understanding and assessing many biogeochemical processes. For example, concentrations of chlorophyll-a and total suspended matter can be used as proxies to assess phytoplankton dynamics and particulate loading. However, quantitative estimation of their concentrations from satellite observations is complicated when working with mixed spectral signatures. Hyperspectral remote sensing is fast emerging as a key technology for advanced and improved understanding of optically complex waters. This study estimates concentrations of chlorophyll-a and total suspended matter (TSM) in the WBLE by applying the partial least squares (PLS) method to a full range (400–900 nm) of continuous narrow spectral bands. The PLS method models the covariance between hyperspectral bands and CPAs, and identifies the optimal bands that characterize most of the variance in the CPAs. This method avoids the curse of dimensionality and the effects of multi-collinearity, a challenge that is associated with new-generation hyperspectral satellite sensors. Validation parameters for the PLS-based models produced R² of 0.84 for chlorophyll-a (RMSE = 1.18 μg/L), and R² of 0.90 for TSM (RMSE = 1.26 mg/L), illustrating the potential of the PLS method for isolating and extracting absorption features characterizing the various CPAs in optically complex Case II type waters.

Editor Z.W. Kundzewicz Associate editor Not assigned     

Shallow sediment transport flow computation using time-varying sediment adaptation length

Based on the common approach,the adaptation length in sediment transport is normally estimated astemporally independent.However,this approach might not be theoretically justified as the process of reaching the sediment transport equilibrium stage is affected by the flow conditions in time,especially for fast moving flows,such as scour-hole developing flows.In this study,the two-dimensional（2D） shallow water formulation together with a sediment continuity-concentration（SCC） model were applied to flow with mobile sediment boundary.A timevarying approach was proposed to determine the sediment transport adaptation length to simulate the sediment erosion-deposition rate.The proposed computational model was based on the Finite Volume（FV） method.The Monotone Upwind Scheme of Conservative Laws（MUSCL）-Hancock scheme was used with the Harten Lax van Leer-contact（HLLC） approximate Riemann solver to discretize the FV model.In the flow applications of this paper,a highly discontinuous dam-break,fast sediment transport flow was used to calibrate the proposed timevarying sediment adaptation length model.Then the calibrated model was further applied to two separate experimental sediment transport flow applications documented in the literature,i.e.a highly concentrated sediment transport flow in a wide alluvial channel and a sediment aggradation flow.Good agreement with the experimental data were obtained with the proposed model simulations.The tests prove that the proposed model,which was calibrated by the discontinuous dam-break bed scouring flow,also performed well to represent rapid bed change and steady sediment mobility conditions.     

Evaluation of the effect of water management interventions on water level of Gaza coastal aquifer

Gaza coastal aquifer (GCA) is the most precious natural source where it is the only source of water for different uses. Groundwater crisis in Gaza includes two major folds: shortage of water supply and contamination. The extraction of groundwater currently exceeds the aquifer recharge rate. As a result, the groundwater level is falling continuously leading severely deterioration of GCA. The main objective of this study is to analyze and evaluate the current and proposed water resources management plans and their effect on the water level of GCA. In this respect, the available quantities of rainfall that could be harvested and infiltrated from different types of land-use based on existing and planned situations are studied using GIS tool and numerical models for GCA using V-MODFLOW environment for simulating four scenarios: (i) existing management practice (no action scenario), (ii) proposed Palestinian Water Authority (PWA) stormwater infiltration plan, (iii) proposed Gaza Emergency Technical Assistance Program (GETAP) interventions, and (iv) combination between second and third scenarios. The management scenarios were tested with the calibrated flow model for the target period between 2016 and 2040. The simulation results of existing management practice scenario show that there are several depression zones in Gaza Strip; in southern part from ??18 to ??24 m MSL in 2020 and 2040, in the northern part from ??7 to ??12 m MSL in 2020 and 2040, and in the middle regions experienced a small decline in groundwater level. The simulation results of proposed PWA scenario indicate similar depression zones as per first scenario but with good enhancement of water level, ??17 to ??18 m MSL in the southern part and ??3 to ??6 m MSL in the northern part in 2020 and 2040, respectively. The simulation results of GETAP intervention scenario show a positive impact on groundwater level. The results of fourth scenario show good enhancement of water level, in which the water level in the northern part ranges from +?3 to +?6 m MSL in 2020 and 2040, while in the south part ranges from ??15 to +?4 MSL in 2020 and 2040.     

Biodegradation of cyanide and evaluation of kinetic models by immobilized cells of <Emphasis Type="Italic">Serratia marcescens</Emphasis> strain AQ07

Immobilized form of Serratia marcescens strain AQ07 was experimented for cyanide biodegradation. Cyanide degradation (200 ppm) was achieved after 24-h incubation. Three parameters were optimized which included gellan gum concentration, beads size, and number of beads. In accordance with one-factor-at-a-time method, cyanide removal was optimum at 0.6% w/v gellan gum gel, 0.3-cm-diameter beads, and 50 beads number. It was able to withstand cyanide toxicity of 800 ppm, which makes it very suitable candidate in cyanide remediation. Beads reusability indicates one-cycle ability. The first cycle removed 96.3%, while the second removed 78.5%. Effects of heavy metals at 1.0 ppm demonstrated that mercury has a considerable effect on bacteria, inhibiting degradation to 61.6%, while other heavy metals have less effect, removing 97–98%. Maximum specific degradation rate of 0.9997 h^?1 was observed at 200 ppm cyanide concentration. Gellan gum was used as the encapsulation matrix. ?-picoline-barbituric acid spectrophotometric analytical method was used to optimize the condition in buffer medium integrated with potassium cyanide via one-factor-at-a-time and response surface method. The range of cyanide concentrations used in this research, specific biodegradation rate was obtained to model the substrate inhibition kinetics. This rate fits to the kinetic models of Teisser, Aiba and Yano, which are utilized to elucidate substrate inhibition on degradation. One-factor-at-a-time approach parameters were adopted because it removes more cyanide compared to response surface methodology modules. The predicted biokinetic constant from this model suggests suitability of the bacteria for use in cyanide treatment of industrial waste effluents.     

Seasonal variation of solar irradiance and sunshine duration in Saudi Arabia

Saudi Arabia is a huge area with diverse climatic and topographical features. This diversity in climate is augmented by seasonal weather variations. The study discusses solar irradiance and sunshine duration in terms of seasonal weather systems. Sunshine duration and solar irradiance maps show that the East Mediterranean weather systems (such as Cyprus low) in winter affect the majority of the geographical regions in Saudi Arabia. In spring and autumn, Sudan low becomes more active than Cyprus low, especially in the west and central regions. Sunshine duration in April is 47% compared to 50% in January. Sunshine duration in the southwest region is affected by the rainy and wet summer monsoon which reduces sunshine duration to 33% in summer (July), while it is 43% in winter (January). In addition, the study uses the Angstrom equation to estimate solar irradiance. The absolute relative error between estimated and observed solar irradiance ranges between 2 and 10% in the majority of cases, with a few cases exceeding 13%.     

Hydrogeophysical investigations for assessing the groundwater potential in part of the Peshawar basin,Pakistan

A large number of valleys and basin systems are present in the northwestern part of the Himalayas in Pakistan which form significant aquifers in the region. Hydrogeophysical investigations in the western part of Nowshera District, a part of the intermontane Peshawar basin, were undertaken to help to determine the availability of groundwater resources in the region. Thirty vertical electrical resistivity soundings (VES) were acquired using a Schlumberger expanding array configuration with a maximum current electrode spacing (AB/2) of 150 m in delineating the groundwater potential in the study area. The results of the interpreted VES data using a combination of curve matching technique and computer iterative modeling methods suggest that the area is underlain by 3 to 5 geo-electric layers. The interpretation results showed that the geo-electrical succession consists of alluvium comprising of alternating layers of clay, silty clay, fine to coarse sands, sand with gravels and gravels of variable thickness. High subsurface resistivity values are correlated with gravel–sand units and low resistivity values with the presence of clays and silts. The modeled VES results were correlated with the pumping tests results and lithological logs of the existing wells. The pumping test suggests the transmissivity of the aquifer sediments is variable corresponding to different sediments within the area. The gravel–sand intervals having high resistivity value show high transmissivity values, whereas clay–silt sediments show low transmissivities. It is concluded that majority of the high resistive gravel–sand sediments belong to an alluvial fan environment. These gravel–sand zones are promising zones for groundwater abstraction which are concentrated in the central part of the study area.     

Temporal shoreline change and infrastructure influences along the southern Red Sea coast of Saudi Arabia

Jizan is one of the Saudi Arabian coastal cities endowed with diverse natural settings, which includes Ash Shuqayq in the north, Turfah in the centre and Jizan in the South. This work analysed specific environmental characteristics, such as spits, sabkhas and wadis. Assessments used Landsat imagery to examine coastal change between 1973 and 2011. The cumulative temporal change identified regression trends given by coefficients of determination that explained a significant percentage of data variation for Jizan (R ² = 69%) and Turfah (R ² = 72%), while Ash Shuqayq was insignificant (R ² = 14%). Inter-survey results predicted future change, although trends were not significant, i.e. Jizan (R ² = 22%), Turfah (R ² = 14%) and Ash Shuqayq (R ² = 3 and 61% with outlying value removed). Aerial photos showed regional coastal changes, which included a maximum accretion of 36.4 m and maximum erosion of 12.9 m. These are scientifically effective techniques to monitor regional coastal change, i.e. erosion and accretion and identified rates of 0.59, 1.80 and 3.53 myr^?1 for Ash Shuqayq, Turfah and Jizan. Changes were linked to infrastructure developments, e.g. tourism, port development and natural causes, e.g. spit formations and wadi outfalls.