青海地区三级GPS大地控制网的建立

针对青海地区地理环境的特点和大地控制网的现状,从GPS控制网设计与布测、数据处理、控制网质量检测和成果管理等方面对青海地区三级GPS大地控制网的建立进行了分析研究.在此基础上,总结了在高原困难地区建立GPS网的一些经验.     

ERDAS IMAGINE在1∶50000土壤底图纠正中的应用

目前利用ERDAS IMAGINE软件进行影像纠正的技术和工艺已日益成熟和完美。通过ERDAS IMAGINE在1∶50000土壤底图纠正中的应用,从生产的角度叙述了ERDAS IMAGINE软件在影像纠正中的关键技术问题,主要包括基础地理信息数据的处理、地面控制点的选取和控制点的判读。     

中国地理信息系统(GIS)发展状况的初步调查——由一组统计数据引起的思考

本文整理和统计了一组与中国GIS研究、教育、产业和市场相关的数据, 用于理解和评估中国的GIS产业状况和应用前景。     

长江沿江地区化工产业空间格局演化及影响因素

基于企业数据和空间计量方法,探讨了长江沿江地区2000—2013年化工产业空间格局演化及影响因素。结果表明：化工产业主要分布在上海市和江苏沿江地区;中上游部分地区化工产业呈现明显的增长势头,由3个主要热点区发展到20余个不同规模大小的分布热点,成为化工产业新兴“增长极”;城市主城区化工产业减少而远郊区县增加,分布热点变化也反映了化工产业“郊区化”“园区化”的过程;相对低端基础的行业呈现集聚趋势,其中肥料业集聚明显;相对高端精细的行业呈现扩散趋势,其中合成材料业分散化明显;地区化工产业增长受到环境规制、外商投资、交通区位等因素显著影响,外商投资和交通区位具有显著的促进作用,而环境规制呈现显著的抑制效果。     

High-throughput sequencing of 16S r RNA amplicons characterizes gut microbiota shift of juvenile sea cucumber Apostichopus japonicus feeding with three antibiotics

Sea cucumber Apostichopus.japonicus is an important marine economic species in Asian countries due to its profound nutritional and medicinal value.So far,with the rapid development of intensified artificial aquaculture of sea cucumbers,the use of antibiotics is still an inexpensive and dispensable way to treat pathogenic infections,especially during the nursery phase.However,there is little information on the effects of antibiotics on the intestinal microbiota of sea cucumber.Therefore an Illumina based sequencing method was used to examine the intestinal bacterial composition of juvenile A.japonicas following diets with three typical antibiotics(tetracycline,erythromycin.,and norfloxacin)under 15,30,and 45 d.The findings reveal that different antibiotics have distinct effects on the growth performance of juvenile sea cucumbers.However,the richness and diversity of microbiota were barely affected by antibiotics but the community composition alterations indicated that the three antibiotics exhibited their respective patterns of reshaping the intestinal bacteria of juvenile sea cucumbers.In common,the abundance of some sensitive genera with helpful functions,such as Thalassotalea,Shewanella,Sulfitobacter,and Halomonas decreased significantly with exposure to antibiotics and the abundance of multiple potential pathogenic-and suspected antibiotic-resistant microorganisms like Arcobacter,Leucothrix,and Clostridium_sensu_stricto_1 was found increased significantly in the antibiotic groups.These results suggest that low doses of antibiotics could affect the composition of the intestinal microbiota of sea cucumbers and might increase the risk of infection of the hosts.This study could help us to explore how antibacterial compounds modify the gut microbiota of sea cucumbers and provide theoretical guidance in hatchery management by scientific antibiotic use in sea cucumber mariculture.     

Fish Assemblage Responses to a Low-head Dam Removal in the Lancang River

Dam removal is becoming an effective approach for aquatic biodiversity restoration in damming river in order to balance the aquatic ecosystem conservation with large-scale cascade damming. However, the effects of dam removal on fish communities in Asian mountainous rivers, which are dominated by Cypriniformes fishes, are still not well known. To determine whether dam removal on a mountainous river benefit restoration of fish diversity, we investigated the response of fish assemblage to dam removal using a before-after-control-impact design in two tributaries of the Lancang River(dam removal river: the Jidu River, and control river: the Fengdian River). Fish surveys were conducted one year prior to dam removal(2012) and three years(2013–2015) following dam removal. We observed rapidly and notably spatio-temporal changes in fish biodiversity metrics and assemblage structure, occurring in the Jidu River within the first year after dam removal. Overall, fish species richness, density and Shannon-Wiener diversity all increased immediately in above-and below-dam sites, and maintained a stable level in subsequent years, compared to unchanged situation in the control river. All sites in the Jidu River experienced shifts in fish composition after dam removal, with the greatest temporal changes occurred in sites below-and above-the former dam, resulting in a temporal homogenization tendency in the dam removed river. These findings suggest that dam removal can benefit the recovery of habitat conditions and fish community in Asian mountainous rivers, but the results should be further evaluated when apply to other dammed rivers since the dam age, fluvial geomorphology and situation of source populations could all affect the responses of fish assemblages.     

Modeling spatially non-stationary land use/cover change in the lower Connecticut River Basin by combining geographically weighted logistic regression and the CA-Markov model

Simulating land use/cover change (LUCC) and determining its transition rules have been a focus of research for several decades. Previous studies used ordinary logistic regression (OLR) to determine transition rules in cellular automata (CA) modeling of LUCC, which often neglected the spatially non-stationary relationships between driving factors and land use/cover categories. We use an integrated geographically weighted logistic regression (GWLR) CA-Markov method to simulate LUCC from 2001–2011 over 29 towns in the Connecticut River Basin. Results are compared with those obtained from the OLR-CA-Markov method, and the sensitivity of LUCC simulated by the GWLR-CA-Markov method to the spatial non-stationarity-based suitability map is investigated. Analysis of residuals indicates better goodness of fit in model calibration for geographically weighted regression (GWR) than OLR. Coefficients of driving factors indicate that GWLR outperforms OLR in depicting the local suitability of land use/cover categories. Kappa statistics of the simulated maps indicate high agreement with observed land use/cover for both OLR-CA-Markov and GWLR-CA-Markov methods. Similarity in simulation accuracy between the methods suggests that the sensitivity of simulated LUCC to suitability inputs is low with respect to spatial non-stationarity. Therefore, this study provides critical insight on the role of spatial non-stationarity throughout the process of LUCC simulation.     

产新琼四糖的海洋微泡菌属AG1热稳定性β-琼胶酶的过量表达与鉴定

从微泡菌属AG1（Microbulbifer sp. AG1）克隆得到1302 bp大小的琼胶酶基因,该基因编码产物为一个成熟蛋白（413个氨基酸残基）外加一个信号肽（20个残基）。将不含信号肽片段的琼胶酶在E. coli BL21 （DE3）中进行了异源表达和纯化。使用琼脂糖作为底物,该重组琼胶酶的最适反应温度和pH分别为60℃和7.5。该重组酶表现出优良的热稳定性,在50℃和60℃下处理1 h,重组琼胶酶仍能分别保持67%和19%的残余酶活力。除了SDS,重组琼胶酶对于其他测试的抑制剂、去垢剂和尿素变性剂有着较好的抗性。利用薄层色谱和以对硝基苯-α/β-D-吡喃半乳糖苷为底物的酶活力分析结果表明,该重组琼胶酶为β型琼胶酶,它水解琼脂糖的主要终产物为新琼四糖,而且不同聚合度的酶解产物具有抗氧化活性。     

黄河三角洲孤东近岸冲淤演变及其影响因素

为更好地了解近40年来孤东近岸的演变过程,以研究区剖面水深地形、Landsat影像和利津站水沙数据为数据源,采用遥感技术及数理统计法对研究区域岸线及面积变化进行监测计算,并分析1976—1986年、1986—1996年、1996—2002年、2002—2014年4个不同阶段的冲淤演变及影响因素。结果表明:(1)孤东近岸经历"强淤积-冲淤平衡-侵蚀-强侵蚀"4个阶段。孤东近岸海域由淤积向侵蚀转变始于1996年,且在2002—2014年间侵蚀最为严重,大部分近岸海域蚀深达到6～8 m,侵蚀最大深度超过8 m;(2)等深线变化时空差异明显,蚀退最先出现在北侧,且近岸5 m水深区域内冲淤变化较水深10 m内敏感;(3)研究区近岸侵蚀,离岸淤积,剖面冲淤平衡位置由CS19剖面的11 m水深变化到CS21剖面的5m水深;(4)黄河入海水沙的减少、河口人工改汊、孤东大堤建设和海洋动力作用都对孤东近岸的冲淤演变产生影响,维持研究区冲淤平衡的年均来沙阈值为3.78亿t/a。通过此来进一步探究孤东近岸演变进程,为孤东近岸防护提供科学指导。     

全球大洋潮汐模式在北印度洋潮汐预报准确性的评估

为评估DTU10、TPXO8、GOT00.2和NAO.99b 4个全球大洋潮汐模式对北印度洋潮汐的预报能力,采用英国海洋资料中心提供的海区中部和沿岸站潮汐调和常数资料,检验了这些模式4个主要分潮(M_2、S_2、K_1、O_1)的准确度。它们的各分潮调和常数资料准确度都比较高,振幅绝均差的最大值仅5.61 cm,迟角绝均差的最大值仅9.13°。这些模式的调和常数给出潮波传播特征差别不大。基于这些模式提供的调和常数,分别建立了北印度洋4、8和16分潮潮汐预报模型,将预报结果与中国海事服务网提供的沿岸24个站潮汐表资料进行对比。各模式的8分潮(M_2、S_2、N_2、K_2、K_1、O_1、P_1、Q_1)潮汐预报模型均优于4分潮(M_2、S_2、K_1、O_1)潮汐预报模型,NAO.99b模式可以提供16分潮(M_2、S_2、N_2、K_2、K_1、O_1、P_1、Q_1、MU_2、NU_2、T_2、L_2、2N_2、J_1、M1、OO_1)潮汐预报模型,但是对预报结果改善不明显;在各模式中,GOT00.2模式的8分潮潮汐预报模型对北印度洋沿岸的预报效果最好,平均绝均差为14.97 cm。