Reproductive success as an indicator of genotoxicity in the polychaete worm, Neanthes arenaceodentata

The effects on reproduction of single or chronic exposure to the direct-acting mutagen, radiation, were evaluated using Neanthes arenaceodentata as the model animal. In the first set of experiments, mated pairs were irradiated when oocytes were visible in the female and were given a single dose of either 0·5, 1·0, 2·0, 5·0, 10 or 50 Gy. In the second set of experiments, lifetime exposure was initiated upon the spawning of the P₁ female and was terminated upon the spawning of the F₁ female; the average total doses (0·55, 6·5 and 54 Cy) were delivered at a rate of 0·19, 2·1 or 17 mGyH⁻¹. Our results on embryo abnormalities and mortalities indicated that lethal mutations were most likely induced in the germ cells and had an adverse effect on reproductive success by reducing the survival of early life stages. Except for those mated pairs exposed acutely to 10 or 50 Gy or exposed chronically to 17 mGyh⁻¹, there was no evidence of gamete killing. However, evidence was obtained for mutagen-damage accumulation in developing gametes from continuous exposure to radiation.     

Isotopic signatures of organic matter in sediments of the continental shelf facing the Orinoco Delta: Possible contribution of organic carbon from savannas

The ⁸⁷Sr, ¹³C, and ¹⁵N isotopic signatures of organic matter in sediments from the continental shelf facing the Orinoco Delta were measured to determine the contribution of sediments transported from the Amazon River by the coastal Guayana current and the sediments transported by the Orinoco River. Box core samples between 60 and 300 m water depth collected along 4 transects located eastwards to the Orinoco Delta were analyzed. Carbon and nitrogen concentrations decreased with depth under water on the shelf, and were strongly correlated indicating homogeneity of organic matter composition. Phosphorus content was also associated to organic matter in most samples, but some of them revealed deposition of P-enriched sediments. The ⁸⁷Sr/⁸⁶Sr ratios showed a strong continental signature averaging 0.7117, therefore, limiting the possible carbon and nitrogen sources associated with these sediments to C3 trees, C4 grasses, or freshwater phytoplankton. The δ¹³C values were relatively high averaging −21‰, above values reported for sediments on the Amapá shelf and the Amazon River in Brazil. Average δ¹³C values did not differ significantly among transects. High δ¹³C values point to the influence of organic matter transported from the C4-plants dominated savannas in the northern fringe of the Orinoco River. δ¹⁵N values were positive and averaged 5‰, being within the range of values measured in the Marajo island (Amazon River) and the estuary of the Pará River. The δ¹⁵N values differed significantly among transects (4.9–5.2‰), lowest values corresponding to the northernmost transect near the coast of Trinidad, and the highest values corresponding to the transect located at the southernmost position.     

A fungal epizootic in mussels at a deep-sea hydrothermal vent

Mass mortalities due to disease are important determinants of population and community structure in marine ecosystems, but the speed at which an epizootic may sweep through a population, combined with rapid selection for disease‐resistant stocks, can mask the ecological impact of disease in all but the most closely monitored populations. We document an emergent epizootic event in the deep sea that is occurring in mussels (Bathymodiolus brevior) at the Mussel Hill hydrothermal vent in Fiji Basin and we identify the causal agent as a black yeast (order Chaetothyriales) that elicits a pronounced host immune response and is associated with tissue deterioration. The yeast was not observed in other invertebrate taxa (the gastropods Ifremeria nautilei, Alviniconcha aff. hessleri; the limpets Lepetodrilus schrolli, Symmetromphalus aff. hageni; the polychaetes Branchipolynoe pettiboneae, Amphisamytha cf. galapagensis) associated with the mussel bed, nor in mussels (Bathymodiolus brevior) collected from adjacent Lau Basin mussel beds. Massive mussel mortality resulting from the fungal infection is anticipated at the Mussel Hill site in Fiji Basin; we expect that epizootic outbreaks in dense invertebrate communities have the potential to be major determinants of community structure in deep‐sea chemosynthetic ecosystems. The possibility that submersible assets may serve as vectors for transport of the fungus warrants further attention.     

Relationship of Caspian tern foraging ecology to nesting success in the Columbia River estuary, Oregon, USA

The prevalence of juvenile salmonids (Oncorhynchus spp.) and marine forage fishes in the diet of Caspian terns (Hydroprogne caspia) nesting in the Columbia River estuary has been established, but the relationship between diet composition, foraging distribution, and productivity of these birds has received little attention. We used radio-telemetry and on-colony observations to relate changes in off-colony distribution to patterns of colony attendance, diet composition, and productivity of adult terns nesting on East Sand Island during two years of different river and prey conditions. Average distance from the East Sand Island colony (located in the marine zone of the estuary) was 38% (6.6 km) greater in 2000 compared to 2001, associated with lower availability of marine forage fish near East Sand Island and lower prevalence of marine prey in tern diets. Colony attendance was much lower (37.0% vs. 62.5% of daylight hours), average trip duration was 40% longer (38.9 min), and nesting success was much lower (0.57 young fledged pair⁻¹ vs. 1.40 young fledged pair⁻¹) in 2000 compared to 2001. Higher proportions of juvenile salmonids in the diet were associated with relatively high use of the freshwater zone of the estuary by radio-tagged terns, which occurred prior to chick-rearing and when out-migrating salmonid smolts were relatively abundant. Lower availability of marine prey in 2000 apparently limited Caspian tern nesting success by markedly reducing colony attendance and lengthening foraging trips by nesting terns, thereby increasing chick mortality rates from predation, exposure, and starvation.     

Measuring low concentrations of Th in water and sediment

²³⁴Th/²³⁸U disequilibria have been used extensively in studies of particle dynamics and the fate and transport of particle-reactive matter in marine environments. Similar work in low salinity, estuarine, and freshwater systems has not occurred primarily because the lower concentrations of both parent and daughter nuclides that are typical of these systems often render established methods for the analysis of ²³⁴Th inadequate. The application of this radionuclide tracer technique to these systems, however, has great potential. To this end, we present a method for measuring low activities of ²³⁴Th in relatively small samples (<200 l) using low background gas-flow proportional counters, a ²²⁹Th yield monitor, and empirical corrections for the interferences from real and apparent betas that are emitted by other thorium isotopes and their progeny. For samples with low ²³⁴Th/²²⁸Th activity ratios, we improve upon current beta counting methodologies that rely on immediate sample counting, weak beta absorption, or multiple beta counts so that, using the analytical approach outlined here, it should be possible to measure ²³⁴Th activities (i) as low as 1.5 dpm/total sample, (ii) up to 2 weeks after radiochemical purification of thorium, and (iii) with only one sample count for alpha and beta activity.     

Combined satellite and ground-based observations of the quiescent,high latitude symbiotic variable AG Draconis=BD+67°922

Satellite observations, fromEinstein,IUE, andIRAS, have been combined with ground-based observations to derive the quiescent energy distribution of the symbiotic star AG Draconis. A detailed comparison is made between the combined observations and various steady-state composite models, including blackbody accretion disks.Paper presented at the IAU Colloquium No. 93 on Cataclysmic Variables. Recent Multi-Frequency Observations and Theoretical Developments, held at Dr. Remeis-Sternwarte Bamberg, F.R.G., 16–19 June, 1986.     

Geochronology and geochemistry of Palaeozoic intrusive rocks in the Rockvale region,southern New England Orogen,New South Wales

Palaeozoic intrusive rocks of the New England Batholith from the Rockvale district in the southern New England Orogen form three distinct associations: (i) the Carboniferous Rockvale Adamellite, a member of the Hillgrove Suite of deformed S‐type granitoids; (ii) a small I‐type igneous complex on the northwestern margin of the Rockvale Adamellite: several members of this complex have similar chemical compositions to the most mafic members of the Moonbi Suite of New England Batholith I‐types; and (iii) a suite of dyke rocks ranging in composition from calc‐alkaline lamprophyre through hornblende and biotite porphyrite to aplite. Ion‐microprobe U‐Pb zircon analysis indicates intrusion of the Rockvale Adamellite at 303 ±3 Ma (weighted mean ²⁰⁶Pb/²³⁸U age; 95% confidence limits). Preliminary investigation of zircon inheritance within the Rockvale Adamellite is consistent with chemical and isotopic indications of derivation of New England Batholith S‐type granitoids from a relatively juvenile protolith. Deformation of the Rockvale Adamellite occurred after complete crystallization of the pluton and prior to emplacement of dykes and I‐type intrusives. K‐Ar biotite and hornblende ages show broadly synchronous intrusion of I‐type magmas and lamprophyre dykes at ca 255 Ma, indicating that mantle magmatism associated with lamprophyres was contemporaneous with the crustal production of I‐type melts. Chemical similarities between the most mafic Moonbi Suite members and calc‐alkaline lamprophyres may also indicate a direct mantle contribution to some I‐type magmas.     

Conductively driven,high‐thermal gradient metamorphism in the Anmatjira Range,Arunta region,central Australia

LA–ICP–MS in situ U–Pb monazite geochronology and P–T pseudosections are combined to evaluate the timing and physical conditions of metamorphism in the SE Anmatjira Range in the Aileron Province, central Australia. All samples show age peaks at c. 1580–1555 Ma, with three of five samples showing additional discrete age peaks between c. 1700 and 1630 Ma. P–T phase diagrams calculated for garnet–sillimanite–cordierite–K‐feldspar–ilmenite–melt bearing metapelitic rocks have overlapping peak mineral assemblage stability fields at ~870–920 °C and ~6.5–7.2 kbar. P–T modelling of a fine‐grained spinel–cordierite–garnet–biotite reaction microstructure suggests retrograde P–T conditions evolved down pressure and temperature to ~3–5.5 kbar and ~610–850 °C. The combined geochronological and P–T results indicate the SE Anmatjira Range underwent high‐temperature, low‐pressure metamorphism at c. 1580–1555 Ma, and followed an apparently clockwise retrograde path. The high apparent thermal gradient necessary to produce the estimated P–T conditions does not appear to reflect decompression of high‐P assemblages, nor is there syn‐metamorphic magmatism or structural evidence for extension. Similar to previous workers, we suggest the high‐thermal gradient P–T conditions could have been achieved by heating, largely driven by high heat production from older granites in the region.