Rapid visualization of plankton abundance and taxonomic composition using the Video Plankton Recorder

Traditional methods for determining spatial distributions of planktonic taxa involve net, pump, and bottle collections followed by the tedious and time-consuming task of plankton sample analysis. Thus, plankton ecologists often require months or even years to process samples from a single study. In this paper, we present a method that allows rapid visualization of the distribution of planktonic taxa while at sea. Rapid characterization of plankton distributions is essential in the dynamic physical environment, where biological and physical patterns can change quickly. Such a “sample-and-observe” capability is necessary for mapping ephemeral features (such as patches, eddies, jets, plumes) and determining appropriate locations to conduct more localized sampling, including in situ observational studies. We describe the techniques used in imaging the plankton, analyzing the video, and visualizing the data. We present an example of at-sea data analysis conducted aboard R.V. Columbus Iselin on Georges Bank in May 1994 and visualizations of the 3-dimensional distribution of selected planktonic taxa in a 2 × 2 km × 90 m volume of seawater. A video of the image processing and visualization is included on the CD-ROM accompanying this volume and is an essential part of this paper.     

Paleomonsoon precipitation deduced from a sediment core from the equatorial Indian Ocean

Rapid shifts in past climate recorded in polar ice sheets have elicited various explanations relating to either thermohaline circulation changes by ice-rafting or natural greenhouse gas concentrations modulated by climatic conditions in the tropics. To compare the tropical paleoclimate record with the polar record, one must choose sediment cores from highly productive ocean regions. Necessarily, such regions reflect the wind records in the tropics, because high productivity is associated with upwelling driven by winds. Comparing tropical precipitation records with high-latitude records is, however, a more difficult task because sediments recording paleoprecipitation usually have low sedimentation rates, and offer coarser resolution relative to polar ice cores. Here, we present δ ¹⁸O data of three planktonic species of Foraminifera (a proxy for precipitation) from such a sediment core, spanning the past 35 ka for the equatorial Indian Ocean, which falls under the southwest monsoon (SWM) realm. Results show that minimum SWM precipitation occurred at the Last Glacial Maximum, with a subsequent increase at Termination IA. During the Holocene, SWM precipitation intensified uniformly up to the core top (∼2.2 ka b.p.), as revealed by generally decreasing δ ¹⁸O values. Variations in precipitation are consistent with climate changes recorded in polar ice sheets. Although the different resolutions of the two records preclude a rigorous comparison, abrupt cooling/warming events appear to be accompanied by sudden reduction/enhancement in (SWM) rainfall. Thus, mechanisms with time scales much shorter than a millennium, such as natural greenhouse warming (e.g., CH₄ concentration), controlled by emissions from the tropics, could have played a major role in high-latitude climate change.     

Pop-up bottom seismic recorder (PUBS) of the Institute of Oceanographic Sciences,U.K.

A pop-up bottom seismic recorder designed for seismic refraction experiments was built by the Institute of Oceanographic Sciences in 1968. The device is housed within a 71 cm diameter sphere weighing 270 kg when launched. signals picked up by a hydrophone are recorded in analogue form on magnetic tape in the band 2–100 Hz. The total continuous recording period is 12 hr but the lifetime of the system can be effectively extended by cycling the tape-recorders to allow shooting to go on for up to 3 days. Ballast release is by acoustic command or by pre-set clock. The instruments have been used in water depths from 150 to 4820 m making a total of 63 deployments with a 95% recovery rate. A new version with three-component geophones is being built.     

The impact of wave loads and pore-water pressure generation on initiation of sediment transport

The build-up of pore-water pressure by waves can lead to sediment liquefaction and subsequent transport by traction currents. This process was investigated by measuring pore-water pressures both in a field experiment and laboratory wave tank tests. Liquefaction was observed in the wave tank tests. The results suggest that sand is less susceptible than silts to wave-induced liquefaction because of the tendency to partially dissipate pore-water pressures. However, previous studies have determined that pore-water pressures must approach liquefaction before current velocities necessary to initiate transport are reduced. Once liquefaction has occurred more sediment can be transported.     

Growth Aspects and Magnesium Carbonate Concentrations of the Reef Building Octocoral Heliopora coerulea (Pallas) after Transplantation

Abstract. An experiment to test C have's hypothesis on temperature dependent MgCO₃ concentrations in marine calcareous skeletons was undertaken by transplanting colonies of Heliopora coerulea. The corals were transferred from a shallow lagoon with high average temperatures to the outer bottom of a fringing reef, 16 m depth, with low average temperatures. Against expectation, a statistically significant increase of average mol % concentrations from 0.48 to 0.54 % for upper growth regions and from 0.49 to 0.62% for lower growth regions was obtained. Temperature records during the experimentation period and the above data indicate that the mol% MgCO₃ concentration in the skeleton of living Heliopora colonies increases when transplanted to an environment with a lower temperature average than that of their original growth site, and with seasonal temperature fluctuations which are smaller in the experimental site than in the control site. Temperature fluctuations at the control site were up to 2.5 times greater than at the experimental site for most of the observation months. This supports the conclusion that the parameters a) minimum temperature and b) temperature stability of the environment are essential for the incorporation of Mg into the carbonate skeleton matrix of marine organisms. Within this study the results of a quantitative chemical analysis of the carbonate skeleton of H. coerulea are presented and growth data of transplanted colonies compared with those from the control site.     

Magnetic anomalies in the canary basin and the Mesozoic evolution of the central North Atlantic

The data from a recent magnetic compilation by Verhoefet al. (1991) off west Africa were used in combination with data in the western Atlantic to review the Mesozoic plate kinematic evolution of the central North Atlantic. The magnetic profile data were analyzed to identify the M-series sea floor spreading anomalies on the African plate. Oceanic fracture zones were identified from magnetic anomalies and seismic and gravity measurements. The identified sea floor spreading anomalies on the African plate were combined with those on the North American plate to calculate reconstruction poles for this part of the central Atlantic. The total separation poles derived in this paper describe a smooth curve, suggesting that the motion of the pole through time was continuous. Although the new sea floor spreading history differs only slightly from the one presented by Klitgord and Schouten (1986), it predicts smoother flowlines. On the other hand, the sea floor spreading history as depicted by the flowlines for the eastern central Atlantic deviates substantially from that of Sundvik and Larson (1988). A revised spreading history is also presented for the Cretaceous Magnetic Quiet Zone, where large changes in spreading direction occurred, that can not be resolved when fitting magnetic isochrons only, but which are evident from fracture zone traces and directions of sea floor spreading topography.Deceased 11 November 1991     

Sources and biogeochemical cycling of particulate selenium in the San Francisco Bay estuary

As part of a study of estuarine selenium cycling, we measured the concentration, chemical form (speciation), and distribution of particulate selenium under various river flow conditions in the North San Francisco Bay (from the Golden Gate to the Sacramento and San Joaquin Rivers). We also conducted laboratory studies on the accumulation of selenium by phytoplankton, the critical first step in the transformation of dissolved to particulate selenium. Total particulate selenium concentration in the North SF Bay was relatively constant between high and low flow periods, ranging spatially from 0.05 to 0.35 nmol l⁻¹ and comprising between 5 and 12% of the total water column selenium inventory. Mean concentrations were generally highest in the Carquinez Strait–Suisun Bay region (salinity 0–17) and lowest in Central Bay. However, selenium content of suspended particles varied with river flow, with higher content during low flow (9.76 ± 4.17 nmol g⁻¹; mean ± sd; n = 67) compared to high flow (7.10 ± 4.24 nmol g⁻¹; n = 39). Speciation analyses showed that most particulate selenium is organic selenide (45 ± 27%), with a smaller proportion (typically <30%) of adsorbed selenite + selenate and a varying proportion (35 ± 28%) of elemental selenium. Based on the amount of elemental selenium in the seston (total suspended material), we calculate that resuspension of estuarine sediments could contribute 29–100% of particulate selenium in the water column. While selenium content of SF Bay seston (>0.4 μm) is relatively unenriched compared to phytoplankton (13.6–155 nmol g⁻¹ dry weight) on a mass basis, when normalized to carbon or nitrogen, seston contains a similar selenium concentration to SF Bay sediments or phytoplankton cultures. SF Bay seston is thus comprised of selenium-rich phytoplankton and phyto-detritus, but also inorganic clay mineral particles that effectively “dilute” total particulate selenium. Selenium concentrations in algal cultures (11 species) exposed to 90 nmol l⁻¹ selenite show relatively large differences in selenium accumulation, with the diatoms, chlorophytes and cryptophytes generally having lower selenium cell content (3.8 ± 2.7 × 10⁻⁹ nmol selenium cell⁻¹) compared to the dinoflagellates (193 ± 73 × 10⁻⁹ nmol selenium cell⁻¹). Because phytoplankton are such a rich (but variable) source of selenium, their dynamics could have a profound effect on the particulate selenium inventory in the North SF Bay.