A preliminary methods comparison for measurement of dissolved organic nitrogen in seawater

Routine determination of dissolved organic nitrogen (DON) is performed in numerous laboratories around the world using one of three families of methods: UV oxidation (UV), persulfate oxidation (PO), or high temperature combustion (HTC). Essentially all routine methods measure total dissolved nitrogen (TDN) and calculate DON by subtracting the dissolved inorganic nitrogen (DIN). While there is currently no strong suggestion that any of these methods is inadequate, there are continuing suspicions of slight inaccuracy by UV methods.This is a report of a broad community methods comparison where 29 sets (7 UV, 13 PO, and 9 HTC) of TDN analyses were performed on five samples with varying TDN and DIN concentrations. Analyses were done in a “blind” procedure with results sent to the first author. With editing out one set of extreme outliers (representing 5 out of 145 ampoules analyzed), the community comparability for analyzing the TDN samples was in the 8–28% range (coefficient of variation representing one standard deviation for the five individual samples by 28 analyses). When DIN concentrations were subtracted uniformly (single DIN value for each sample), the comparability was obviously worse (19–46% cv). This comparison represents a larger and more diverse set of analyses, but the overall comparability is only marginally better than that of the Seattle workshop of a decade ago. Grouping methods, little difference was seen other than inconclusive evidence that the UV methods gave TDN values for several of the samples higher than HTC methods. Since there was much scatter for each of the groups of methods and for all analyses when grouped, it is thought that more uniformity in procedures is probably needed. An important unplanned observation is that variability in DIN analyses (used in determining the final analyte in most UV and PO methods) is essentially as large as the variability in the TDN analyses.This exercise should not be viewed as a qualification exercise for the analysts, but should instead be considered a broad preliminary test of the comparison of the families of methods being used in various laboratories around the world. Based on many independent analyses here, none of the routinely used methods appears to be grossly inaccurate, thus, most routine TDN analyses being reported in the literature are apparently accurate. However, it is not reassuring that the ability of the international community to determine DON in deep oceanic waters continues to be poor. It is suggested that as an outgrowth of this paper, analysts using UV and PO methods experiment and look more carefully at the completeness of DIN conversion to the final analyte and also at the accuracy of their analysis of the final analyte. HTC methods appear to be relatively easy and convenient and have potential for routine adoption. Several of the authors of this paper are currently working together on an interlaboratory comparison on HTC methodology.     

Holocene lake development and glacial-isostatic uplift at Lake Skallen and Lake Oyako,Lützow-Holm Bay,East Antarctica: Based on biogeochemical facies and molecular signatures

The paleolimnology of two lakes which were isolated as a result of the crustal uplift during the late Holocene along the Soya Coast, Lützow-Holm Bay, East Antarctica were studied. The focus was on temporal variations in the biogeochemical composition of sediment cores recovered from Lake Skallen at Skallen and Lake Oyako at Skarvsnes. Both sets of lake sediments record environmental changes associated with a transition from marine to lacustrine settings, as indicated by analyses of C and N contents, nitrogen isotopic compositions (δ¹⁵N), and major element concentrations. Changes in the dominant primary producers during the marine–lacustrine transition (marine diatom to cyanobacteria) at L. Skallen was clearly revealed by biogenic opal-A, diatom assemblages, and molecular signature from denaturing gradient gel electrophoresis (DGGE) with 16S ribosomal RNA (rRNA) gene analysis. Radiocarbon dating of acid-insoluble organic C suggested that the environmental transition from marine to fresh water occurred at 2940 ± 100 cal yr BP at L. Skallen and 1060 ± 90 cal yr BP at L. Oyako. Based on these data, a mean crustal uplift rate of 3.2 mm yr⁻¹ is inferred for the history of marine–lacustrine transition via brackish conditions. The geological setting causing glacio-isostatic uplift was the primary factor in controlling the transition event in sedimentary and biological facies.     

Structural controls on the 1998 volcanic unrest at Iwate volcano: Relationship between a shallow, electrically resistive body and the possible ascent route of magmatic fluid

Magnetotelluric (MT) measurements were conducted at Iwate volcano, across the entirety of the mountain, in 1997, 1999, 2003, 2006, and 2007. The survey line was 18 km in length and oriented E–W, comprising 38 measurements sites. Following 2D inversion, we obtained the resistivity structure to a depth of 4 km. The surface resistive layer (~ several hundreds of meters thick) is underlain by extensive highly conductive zones. Based on drilling data, the bottom of the highly conductive zone is interpreted to represent the 200 °C isotherm, below which (i.e., at higher temperatures) conductive clay minerals (smectite) are rare. The high conductivity is therefore mainly attributed to the presence of hydrothermally altered clay. The focus of this study is a resistive body beneath the Onigajo (West-Iwate) caldera at depths of 0.5–3 km. This body appears to have impeded magmatic fluid ascent during the 1998 volcanic unrest, as inferred from geodetic data. Both tectonic and low-frequency earthquakes are sparsely distributed throughout this resistive body. We interpret this resistive body as a zone of old, solidified intrusive magma with temperatures in excess of 200 °C. Given that a similar relationship between a resistive body and subsurface volcanic activity has been suggested for Asama volcano, structural controls on subsurface magmatic fluid movement may be a common phenomenon at shallow levels beneath volcanoes.     

Sustainability of groundwater in Mali,West Africa

The following paper describes the goals and some preliminary work in the Bani sustainability study, an ongoing project in Mali, West Africa. Rural communities in Mali are increasingly relying on hand-pumps, which tap groundwater resources, as a means of obtaining potable water. The long-term sustainable yield of groundwater resources is not known but can be evaluated in sustainability study. In 2005, a groundwater sustainability study was established along the Bani River of Mali. The Bani study collected groundwater levels that were used in a conceptual groundwater flow model—the Bani model—to develop an understanding of current aquifer conditions and to make limited predictions of sustainability under various future scenarios. The Bani model showed the climatic parameters of recharge (derived from precipitation) and evapotranspiration to influence simulated groundwater levels and groundwater volume available, while increased pumping rates, due to population growth, showed little effect. When considered in the context of the actual Bani sustainability study area, the change in groundwater levels resulting from climatic parameters may have negative implications, especially during several consecutive years of decreased precipitation, such as drought, or if downward trends anticipated for precipitation continue.     

Rheological properties and structural changes in different sections of boiled abalone meat

1　Introduction　AbaloneHaliotisdiscusisaverypopularfoodthrough outtheworld .WhenAsianpeoples ,notablyJapaneseandChinese ,eatabalonedishes ,theyenjoythefirmtextureoftherawmeatandthetendernessoftheboiledmeat .InJapan ,thetoughervarietiesoftherawabalonemeatareenjoyedandtheabaloneisoftenthin lyslicedandservedraw (sashimi) ,whileinchina ,thetendernessandsoftervarietiesoflesscollagenofboiledmeatareconsideredsuitableforeating ,sotheabaloneisoftenservedbyheating .Infoodscience ,manyreportshavebeen …     

A flare model deduced from Hinotori and millimeterwave interferometer observations

The -ray and white-light flare of 13 May, 1981 is used for a study of spatial distributions of energetic electrons and high-temperature plasma.     

Diurnal variability of thermospheric N and NO

A model for diurnal variations of neutral and ionic nitrogen compounds in the thermosphere is reconstructed on the basis of a new photochemical aspect on N(²D), together with new observations of the NO density. The NO density so far measured must be reduced by a factor 2, due to a revision of the fluorescence coefficient for the NO γ-band airglow. Incorporating the quenching reaction of N(²D) with O in the model calculation results in a reduction of the NO density at heights as low as 100 km. These two effects are combined to lead to an evaluation that the N(²D) quantum yield for various possible reactions is as large as 0.9. A smaller rate coefficient for the quenching reaction than that measured in the laboratory, i.e. 1.0 × 10^?12cm³sec^?1 is favourable for the recent NO observation in the early morning, as well as the observed emission rates of the 5200 A airglow from N(²D) The present model predicts a significant day-to-night variation of N and NO densities at heights above 100 km. Below 100 km, the NO density is fairly stable because of its long chemical time constant. Since the rate coefficient for the conversion of N(⁴S) to NO is highly temperature dependent, the relative population of N(⁴S) and NO is very sensitive to the thermospheric temperature variation. Large variations of both N(⁴S) and NO densities due to the temperature change could occur especially at night. The model is in good agreement with the NO observations so far available in low and middle latitudes, as well as the N observation by the use of a rocket in the twilight.     

A new interpretation of the 1304 Å triplet airglow intensity ratio with the fine structure levels O3Pj) in local thermodynamic equilibrium

The intensity ratios of the 1304 Å triplet airglow of atomic oxygen observed by Fastie and Crosswhite (1964) are interpreted on the basis of the radiative transfer formulation for a model with complete frequency redistribution in a Voigt line profile. A model for the fine structure levels $\text{O(}{}^3\text{P}{}_{\mathrm{j}}\text{)}$ in local thermodynamic equilibrium is favorable to the observed intensity ratios, as far as a Voigt profile is applicable. In view of large cross sections as calculated theoretically by Allison and Burke (1969), the mutual relaxation among the ³P_j levels should occur rapidly enough to allow the population in the ³P_j levels to be in thermodynamic equilibrium with the ambient neutral gases.     

Researches on the stability of porphyra-334 solution and its influence factors

1　Introduction　Ultravioletradiationisahighlyactivecomponentofsolarspectrum .Ultravioletradiationhasdetrimentaleffectsonlivingthingsexposedtosunlight ,includingthedestructionofDNA ,proteinsandothermolecules(Harm ,1980 ) ,inhibitionofphotosynthesisandgrow…     

Detailed Observation of Spatial Abundance of Clam Larva Ruditapes philippinarum in Tokyo Bay, Central Japan

The spatial distribution of the larval abundance of the clam Ruditapes philippinarum has been investigated at 65 stations throughout Tokyo Bay on August 2, 2001. The large number of small D-shaped larvae that were found shortly after hatching in the waters around the Banzu, Futtu, and Sanmaizu-Haneda areas indicates that spawning populations in these areas probably contribute greatly to the larval supply in the bay. Small larvae also occurred abundantly around the Yokohama and Ichihara port areas, suggesting that these port regions play a role in the transport of larvae into Tokyo Bay. This revised version was published online in July 2006 with corrections to the Cover Date.