Does heavy oil pollution induce bacterial diseases in Japanese flounder Paralichthys olivaceus?

As basic research for the effect of heavy oil on the fish immune system, in this study, the number of leukocyte was counted in Japanese flounder Paralichthys olivaceus, after exposure to heavy oil at a concentration of 30 g/8L for 3 days. To compare the numbers of bacteria in the skin mucus between oil-exposed and control fish, viable bacteria were enumerated by counting colony forming unit (CFU). Compared with 5.79+/-1.88 x 10(7)leukocytes/mL in the controls, the exposed fish demonstrated higher counts, averaging 1.45+/-0.45 x 10(8)cells/mL. The bacterial numbers of control fish were 4.27+/-3.68 x 10(4)CFU/g, whereas they were 4.58+/-1.63 x 10(5)CFU/g in the exposed fish. The results suggest that immune suppression of the fish occurred due to heavy oil stressor, and bacteria could invade in the mucus, resulting in the increasing leukocyte number to prevent infectious disease.     

Process of the Tsushima Current formation revealed by ADCP measurements in summer

In order to clarify detailed current structures over the continental shelf margin in the East China Sea, ADCP measurements were carried out in summers in 1991 and 1994 by the quadrireciprocal method (Katoh, 1988) for removing diurnal and semidiurnal tidal flows from observed flows, together with CTD measurements. We discussed the process of the Tsushima Current formation in the East China Sea. The Tsushima Current with a volume transport of 2 Sv (1 Sv=10⁶ m³s^–1) was found north of 31°N. A current with a volume transport of 0.4 Sv was clearly found along the 100 m isobath. Between the Kuroshio and the current along the 100 m isobath, southeastward component of velocity was dominant compared to northwestward one. Four eastward to southeastward currents were found over the sea bed shallower than 90 m depth. Total volume transport of these four currents was 1 Sv, and they seemed to be originated from the Taiwan Strait. Intrusion of offshore water into the inner shelf northwest of Amami Oshima was estimated to have a volume transport of 0.6 Sv. It is concluded that the Tsushima Current is the confluence of these currents over the continental shelf margin with the offshore water intruding northwest of Amami Oshima.     

U–Pb zircon ages of the Nakanogawa Group in the Hidaka Belt,northern Japan: Implications for its provenance and the protolith of the Hidaka metamorphic rocks

Zircon U–Pb ages of two acidic tuff and two turbidite sandstone samples from the Nakanogawa Group, Hidaka Belt, were measured to estimate its depositional age and the development of the Hokkaido Central Belt, northeast Japan. In the northern unit, homogeneous zircons from pelagic acidic tuff from a basal horizon dated to 58–57 Ma, zircons from sandstone from the upper part of the unit dated to 56–54 Ma, and zircons from acidic tuff from the uppermost part dated to 60–56 Ma and 69–63 Ma. Both of the tuff U–Pb ages are significantly older than the youngest radiolarian fossil age (66–48 Ma). Therefore, the maximum depositional age of the turbidite facies in the northern unit is 58 Ma and the younger age limit, estimated from the fossil age, is 48 Ma. In the southern unit, homogeneous zircons from turbidite sandstone dated to 58–57 Ma. Thus the depositional age of this turbidite facies was interpreted to be 66–56 Ma from the fossil age, probably close to 57 Ma. Most of the zircon U–Pb ages from the Nakanogawa Group are younger than 80 Ma, with a major peak at 60 Ma. This result implies that around Hokkaido volcanic activity occurred mainly after 80 Ma. Older zircon ages (120–80 Ma, 180–140 Ma, 340–220 Ma, 1.9 Ga, 2.2 Ga, and 2.7 Ga) give information about the provenance of other rocks in the Hidaka Belt. It is inferred that the Nakanogawa Group comprises protoliths of the upper sequence of the Hidaka Metamorphic Zone, which therefore has the same depositional age as the Nakanogawa Group (66–48 Ma). The depositional ages of the lower sequence of the Hidaka Metamorphic Zone and the Nakanogawa Group are probably the same.     

日本掀起对活断层定义和地震预报的热烈讨论

１９９７年１１月由日本地震学会宣传委员会发起的英文简称为ｎｆｍｌ的活动至１９９８年２月初已２个多月了。截至１９９８年２月５日,已有１４５名参与者。他们就地震、防灾、科学教育和媒体的作用进行了内容广泛的讨论。参与者中不仅有地震学专家,还有宣传、教育、行...     

Enrichment of alkanes within a phytoplankton bloom during an in situ iron enrichment experiment in the western subarctic Pacific

During the Subarctic Pacific Iron Experiment for Ecosystem Dynamics Study ΙΙ (SEEDS-II), we monitored variations in the concentrations of non-methane hydrocarbons (NMHCs), CH₃Cl, N₂O, and CH₄ within a phytoplankton bloom. Stable isotopic compositions were also determined to evaluate the sources of the variations. Although there was little variation in either the concentrations or the stable isotopic compositions of alkenes, CH₃Cl, N₂O, and CH₄ during the 23-day observation period, alkane concentrations increased substantially as the phytoplankton bloomed. The column-integrated quantities of alkanes increased to 3 times pre-bloom levels for C₂H₆, 5 times for C₃H₈, and 20 times for n-C₄H₁₀. The δ¹³C values of both C₂H₆ and C₃H₈ remained almost constant while concentrations increased, whereas that of n-C₄H₁₀ increased by about 12‰. To evaluate the sources of the alkanes produced during the bloom, we compared their δ¹³C values with those of alkanes produced in axenic phytoplankton cultures in our laboratory. We concluded that during the SEEDS-ΙΙ experiment the major portions of C₂H₆ and C₃H₈ were produced during the autolysis of diatoms cells, whereas n-C₄H₁₀ was produced during autolysis of other phytoplankton cells such as cryptophytes and dinoflagellates.     

The linear stability of the post-Newtonian triangular equilibrium in the three-body problem

Continuing a work initiated in an earlier publication (Yamada et al. in Phys Rev D 91:124016, 2015), we reexamine the linear stability of the triangular solution in the relativistic three-body problem for general masses by the standard linear algebraic analysis. In this paper, we start with the Einstein–Infeld–Hoffmann form of equations of motion for N-body systems in the uniformly rotating frame. As an extension of the previous work, we consider general perturbations to the equilibrium, i.e., we take account of perturbations orthogonal to the orbital plane, as well as perturbations lying on it. It is found that the orthogonal perturbations depend on each other by the first post-Newtonian (1PN) three-body interactions, though these are independent of the lying ones likewise the Newtonian case. We also show that the orthogonal perturbations do not affect the condition of stability. This is because these do not grow with time, but always precess with two frequency modes, namely, the same with the orbital frequency and the slightly different one due to the 1PN effect. The condition of stability, which is identical to that obtained by the previous work (Yamada et al. 2015) and is valid for the general perturbations, is obtained from the lying perturbations.     

Seasonal and spatial variations in the partial pressure of carbon dioxide in a eutrophic brackish lake,Lake Hamana,Japan

The dissolved inorganic carbon and total alkalinity in the surface brackish waters of Lake Hamana were investigated monthly from October 2017 to September 2019 at 14 stations. The partial pressure of carbon dioxide (pCO₂) in the surface water ranged from 29 to 1476 μatm and was undersaturated for atmospheric CO₂ during the observation periods, although most coastal waters were net source areas because of the large amount of terrestrial organic and inorganic carbon input. Since there was a strong negative correlation between pCO₂ and the dissolved oxygen, seasonal and temporal variations in pCO₂ were mainly derived from phytoplankton activity. The high phytoplankton activity induced by the effluents from sewage treatment plants, which was low in carbon and high in nitrogen. Therefore, in urbanized coastal waters with sewage treatment plants, such as the coastal waters of Japan, there is a possibility of shifting from weaker carbon dioxide source areas to sink areas. However, pCO₂ was oversaturated at the polluted river mouth, especially after high precipitation events due to the large carbon supply.

     

Contamination status of POPs and BFRs and relationship with parasitic infection in finless porpoises (Neophocaena phocaenoides) from Seto Inland Sea and Omura Bay, Japan

Contamination status of brominated flame retardants (BFRs) and persistent organic pollutants (POPs) in blubber of finless porpoises (Neophocaena phocaenoides) stranded along the coasts of Seto Inland Sea and Omura Bay in Japan were investigated. Levels of PCBs, DDTs and CHLs were significantly higher than those of HCHs, HCB, PBDEs and HBCDs. Concentrations of PBDEs and HBCDs, as well as organochlorine compounds in males increased with body length (p<0.05). Among 14 PBDE congeners analyzed, BDE-47 was the predominant, which is similar to those generally reported in biota. PBDEs, HBCDs and PCBs showed no obvious temporal trend in concentrations during the study period, suggesting continuous environmental release of these chemicals. On the other hand, levels of DDT, CHLs and HCHs have decreased. Concentrations of PCBs in liver trematode infected individuals were significantly higher than those in not infected individuals, implying there could be a relationship between contaminant levels and parasitic infection.