Isolation,biodegradation ability and molecular detection of hydrocarbon degrading bacteria in petroleum samples from a Brazilian offshore basin

The detection of microorganisms with potential for biodeterioration and biodegradation in petroleum fields is of great relevance, since these organisms may be related to a decrease in petroleum quality in the reservoirs or damage in the production facilities. In this sense, petroleum formation water and oil samples were collected from the Campos Basin, Brazil, with the aim of isolating microorganisms and evaluating their ability to degrade distinct classes of hydrocarbon biomarkers (9,10-dihydrophenanthrene, phytane, nonadecanoic acid and 5α-cholestane). Twenty eight bacterial isolates were recovered and identified by sequencing their 16S rRNA genes. Biodegradation assays revealed that bacterial metabolism of hydrocarbons occurred through reactions based on oxidation, carbon–carbon bond cleavage and generation of new bonds or by the physical incorporation of hydrocarbons into microbial cell walls. Based on the biodegradation results, selective PCR-based systems were developed for direct detection in petroleum samples of bacterial groups of interest, namely Bacillus spp., Micrococcus spp., Achromobacter xylosoxidans, Dietzia spp. and Bacillus pumilus. Primer sets targeting 16S rRNA genes were designed and their specificity was confirmed in silico (i.e. computational analysis) and in PCR reactions using DNA from reference strains as positive and negative controls. Total DNA from oil was purified and the amplification tests revealed the presence of the target bacteria in the samples, unraveling a significant potential for petroleum deterioration in the reservoirs sampled, once proper conditions are present for hydrocarbon degradation. The application of molecular methods for rapid detection of specific microorganisms in environmental samples would be valuable as a supporting tool for the evaluation of oil quality in production reservoirs.     

Zur Deutung der Vertikalbewegungen der Festländer und Meere

Ohne Zusammenfassung     

Climate-smart agriculture: perspectives and framings

This paper offers a systematic analysis of the concepts and contexts that frame the climate-smart agriculture (CSA) discourse in the academic and policy literature. Documents (n?=?113) related to CSA and published in peer-reviewed journals, books, working papers, and scientific reports from 2004 to 2016 were reviewed. Three key trends emerged from the analysis: studies are biased towards global policy agendas; research focuses on scientific and technical issues; and the integration of mitigation, adaptation, and food security (the three pillars of CSA) is becoming a popular scholarly solution. Findings suggest that CSA is a fairly new concept used to describe a range of adaptation and mitigation practices without a specific set of criteria. Although CSA is often framed around the three pillars, the underlying issues constructing the discourse differ at global, developing, and developed country scales. Although there is increasing research on developing countries, particularly in relation to how CSA can transform smallholder agriculture, there is a paucity of research documenting the experiences from developed countries. The findings suggest that research on CSA needs to move beyond solely focussing on scientific approaches and only in certain geographical contexts. If CSA is to be applicable for farmers across the globe, then cross-disciplinary research that is underpinned by broad socio-economic and political contexts is essential to understand how differences in narratives might affect implementation on-the-ground in both developing and developed countries.

POLICY RELEVANCE

Although policy makers are increasingly supportive of the climate-smart agriculture (CSA) approach, the rhetoric has largely been developed on the basis of scientific and technical arguments. The political implications of varying perspectives have resulted in a growing divide between how developing and developed countries frame solutions to the impacts of climate change on agriculture under the 2015 Paris Agreement. Different framings are part of the explanation for why the scope of CSA is being rethought, with the scientific community redirecting attention to seeking a separate work programme under the United Nations Framework Convention on Climate Change (UNFCCC). The current policy framing of CSA will give no new policy direction unless it grounds itself in the smallholder farmer and civil society contexts.     

Learning from CDM SD tool experience for Article 6.4 of the Paris Agreement

The Paris Agreement (PA) emphasizes the intrinsic relationship between climate change and sustainable development (SD) and welcomes the 2030 agenda for the global Sustainable Development Goals (SDGs). Yet, there is a lack of assessment approaches to ensure that climate and development goals are achieved in an integrated fashion and trade-offs avoided. Article 6.4 of the PA introduces a new Sustainable Mitigation Mechanism (SMM) with the dual aim to contribute to the mitigation of greenhouse gas emissions and foster SD. The Kyoto Protocol’s Clean Development Mechanism (CDM) has a similar objective and in 2014, the CDM SD tool was launched by the Executive Board of the CDM to highlight the SD benefits of CDM activities. This article analyses the usefulness of the CDM SD tool for stakeholders and compares the SD tool’s SD reporting requirements against other flexible mechanisms and multilateral standards to provide recommendations for improvement. A key conclusion is that the Paris Agreement’s SMM has a stronger political mandate than the CDM to measure that SD impacts are ‘real, measurable and long-term’. Recommendations for an improved CDM SD tool are a relevant starting point to develop rules, modalities, and procedures for SD assessment in Article 6.4 as well as for other cooperative mitigation approaches.

POLICY RELEVANCE

Research findings are relevant for developing the rulebook of modalities and procedures for Article 6.4 of the Paris Agreement, which introduces a new mechanism for mitigation of greenhouse gas emissions and sustainable development. Lessons learnt from the CDM SD tool and recommendations for enhanced SD assessment are discussed in context of Article 6 cooperative approaches, and make a timely contribution to inform negotiations on the rulebook agreed by the Conference of the Parties serving as the Meeting of the Parties to the Paris Agreement.     

Does Magnetic Flux Submerge at Flux Cancelation Sites?

Simultaneous measurements of the magnetic fields in the photosphere and chromosphere were used to investigate if magnetic flux is submerging at sites between adjacent opposite polarity magnetic network elements in which the flux is observed to decrease or `cancel'. These data were compared with chromospheric and coronal intensity images to establish the timing of the emission structures associated with these magnetic structures as a function of height. We found that most of the cancelation sites show either that the bipole is observed longer in the photosphere than in the chromosphere and corona (44%) or that the timing difference of the disappearance of the bipole between these levels of the atmosphere is unresolved. The magnetic axis lengths of the structures associated with the cancelation sites are on average slightly smaller in the chromosphere than the photosphere. These observations suggest that magnetic flux is retracting below the surface for most, if not all, of the cancelation sites studied.     

Coming to Terms with “Integrated Coastal Management”: Problems of Meaning and Method in a New Arena of Resource Regulation

This paper traces the emergence of coastal management in the late twentieth century and assesses the social and spatial implications of the new Integrated Coastal Management (ICM) philosophy that guides national regulatory programs worldwide. A review of the epistemology of ICM reveals its link to the United Nations marine regulatory regime (the Law of the Sea) and the sustainable development paradigm embraced at the 1992 United Nations Conference on Environment and Development. I suggest that the resulting regulatory regime facilitates the opening of coastal zones worldwide to aggressive state and global capital investment. By promoting the overhaul of existing social and spatial organization in coastal zones and by asserting the primacy of resource access for modern economic interests, ICM may introduce more rather than less social conflict and ecological degradation. To illustrate this dynamic, I examine the case of coral reef management in general, and in the context of the Sri Lankan ICM program.     

Chronology of late Holocene climatic events in the northern North Atlantic based on AMS 14C dates and tephra markers from the volcano Hekla,Iceland

A combination of AMS¹⁴C dating and tephrochronology has been used to date late Holocene oceanographic events in a 335 cm marine record, covering about 4600 cal. yr with sedimentation rates exceeding 80 cm 1000 yr⁻¹. The core site is located 50 km offshore on the northern Icelandic shelf. Tephra markers from Iceland serve to correlate the marine and terrestrial records. Especially notable is the presence of three geochemically correlated tephra markers from the Icelandic volcano Hekla (Hekla 4, Hekla 3 and Hekla 1104). Benthic and planktonic foraminiferal abundance and distribution as well as the petrography of the sand fraction of the muddy shelf sediments are used as palaeoceanographic proxies. The foraminiferal assemblages reflect a general cooling trend during the last 4600 yr. A marked drop in sea‐surface temperatures is registered at about 3000 cal. yr BP, corresponding to the level of the Hekla 3 tephra. There is faunal indication of temperature amelioration during the Medieval Warm Period and a cooling again during the Little Ice Age. Periods of ice rafting events are indicated by ice rafted debris (IRD) concentrations, e.g. at around 3000 cal. yr BP and during the Little Ice Age. The former event occurred just prior to the deposition of the Hekla 3 tephra marker, the largest Holocene Hekla eruption. A correlation with terrestrial climatic events in Iceland is presented. A standard marine reservoir correction of 400 ¹⁴C yr appears to be reasonable, at least during periods with high influence of water masses from the Irminger Current on the northern Icelandic shelf. An increase to ca. 530 ¹⁴C yr may have occurred, however, when water masses derived from the East Greenland Current were dominant in the area. Copyright © 2000 John Wiley & Sons, Ltd.