Polyclonal antibodies to light-harvesting CHL-protein of PSII (LHC II) in marine green algaeBryopsis corticulans

Polyc·lonal antibodies raised against LHC II isolated from SDS-solubilizedBryopsis corticulans thylakiod membranes by SDS-PAGE, were characterised by double immunodiffusion, Rocket immunoelectrophoresis and antigen-antibody crossed immunoelectro-phoresis assays showed the antibodies had strong cross-reaction with allB. corticulans LHC II components (even with those which were incubated in boiling water) and showed immunological cross-reactivity with LHC II polypeptides of spinach and the marine green algaCodium fragile. The results suggested that LHC II of different species had similar antigenic determinants and also conservation of amino acid sequences of the polypeptides during evolution, and that the antibodies could cross react with apoproteins of D₂ proteins (which contain P₆₈₀) fromB. corticulans, spinach andC. fragile, but not with apoproteins of P₇₀₀ Chl-proteins. Our results indicated some similarities in primary structure between LHC II of different species, and between LHC II and D₂ proteins of marine green algae and spinach. Our finding that D₂ and P₇₀₀ Chl-proteins are not immunologically related suggested that P₇₀₀ Chl-proteins and D₂ proteins pass through independent evolutionary pathways. Contribution No 1717 from the Institute of Oceanology, Academia Sinica, This subject was supported by the National Science Foundation of China.     

On a new species ofPavlova (Prymnesiophyceae) from China

Pavlova viridis sp. nov. is described on the basis of light and electron microscope observations. The material was collected from the coast of haiyang county of Shandong, China. It is characterized by 1) yellowish green to green chloroplast, 2) rudimentary short flagellum 0.3 μm long and hook-shaped, 3) long flagellum with small spherical knob-scales, 4) absence of pyrenoid and stigma, and 5) marine habitat. This new species andP. salina seem to be most closely related to each other but their colour, the insertion of their three appendages, the shape of their knob scales, and their cell periplasts are quite different from one another. Contribution No. 1879 from the Institute of Oceanology, Academia Sinica     

Filtering vibroseis data in the precorrelation domain

Vibroseis data recorded at short source–receiver offsets can be swamped by direct waves from the source. The signal-to-noise ratio, where primary reflections are the signal and correlation side lobes are the noise, decreases with time and late reflection events are overwhelmed. This leads to low seismic resolution on the vibroseis correlogram. A new precorrelation filtering approach is proposed to suppress correlation noise. It is the ‘squeeze-filter-unsqueeze’ (SFU) process, a combination of ‘squeeze’ and ‘unsqueeze’ (S and U) transformations, together with the application of either an optimum least-squares filter or a linear recursive notch filter. SFU processing provides excellent direct wave removal if the onset time of the direct wave is known precisely, but when the correlation recognition method used to search for the first arrival fails, the SFU filtering will also fail. If the tapers of the source sweeps are badly distorted, a harmonic distortion will be introduced into the SFU-filtered trace. SFU appears to be more suitable for low-noise vibroseis data, and more effective when we know the sweep tapers exactly. SFU requires uncorrelated data, and is thus cpu intensive, but since it is automatic, it is not labour intensive. With non-linear sweeps, there are two approaches to the S,U transformations in SFU. The first requires the non-linear analytical sweep formula, and the second is to search and pick the zero nodes on the recorded pilot trace and then carry out the S,U transformations directly without requiring the algorithm or formula by which the sweep was generated. The latter method is also valid for vibroseis data with a linear sweep. SFU may be applied to the removal of any undesired signal, as long as the exact onset time of the unwanted signal in the precorrelation domain is known or determinable.     

Symbiotic Binary YY Her – Eclipses, Flares and Outbursts

The long-term CCD and photoelectric observations of YY Her covering the period of its post-outburst activity (JD 2 451 823 -- 2 452 996) are presented. We explain the periodic variations of the brightness of YY Her by the eclipses of the components in the system. The model with a deformed (non-homogeneous) envelope, surrounding the white dwarf is discussed. In addition, we observed a flare in about JD 2 452 440, during the primary minimum, that was later followed by an energetic outburst in JD 2 452 700.     

Estimation of parameters of the flows forming sediment waves on the western slope of the middle caspian sea

By means of mathematical modeling, the parameters of flows forming sedimentary waves on the western slope of the Derbent basin were estimated. The height of these flows depends on the slope steepness and varies from 25 to 170 m to reach its maximum values at gentler slope areas. However, the flow rate is independent of the slope steepness and depends only on the concentration of sediment matter supplied by the flow. At the upper part of the slope (the flow starting), the rate amounts to 0.4–1.4 m/s, being almost halved at the depths where the sedimentary waves are damped. The present rates of near-bottom currents show pronounced seasonal differences, and their values are close to flow rates obtained by numerical modeling.     

Mineralogy and PT formation conditions of lead-zinc ore at the Dzhimidon deposit, north Ossetia

The mineralogy and PT formation conditions of the Dzhimidon Pb-Zn deposit in the Sadon ore district are considered. The deposit is localized in metamorphic rocks of the Buron Formation, which pertain to the pre-Jurassic basement (lower structural stage) and are cut through by Upper Paleozoic granitoids, and in the Lower Jurassic terrigenous sequence (upper structural stage). Orebodies as quartz-sulfide veins are mainly hosted in the metamorphic rocks. Galena, sphalerite, chalcopyrite, pyrite, pyrrhotite, and arsenopyrite are the most abundant sulfides, while quartz, carbonates, chlorite, sericite, and feldspar are gangue minerals. The bismuth mineralization identified at this deposit for the first time is represented by diverse phases of the Ag-Pb-Bi-S system. Five stages of the ore deposit formation are recognized: a premineral stage (quartz-feldspar), three ore-bearing stages (pyrite-arsenopyrite, pyrrhotite-chalcopyrite-sphalerite, and arsenopyrite-sphalerite-galena), and a postmineral stage (quartz-calcite); each stage comprises one or several mineral assemblages. The study of fluid inclusions in quartz, calcite, and sphalerite of the premineral, ore-forming, and postmineral stages has shown that the ore was deposited mainly from Na chloride solution with a salinity varying from >22 to <1.0 wt % NaCl equiv at a temperature from 460 to ～120°C and 430–290 bars pressure. The third stage was characterized by an abrupt increase in temperature and by the appearance of Mg(Fe,Ca) chloride solutions equally with Na chloride fluids, presumably owing to the emplacement of granite porphyry.     

Soil-structure interaction experiment using impulse response

A new experimental method using a finite soil model with no special treatement on its boundaries is employed for soil-structure intration problems to simulate the semi-infinitenesss of the actual soil medium. The present method utlizies the characteristics of transient response to an impluse load to obtain the impedance functions and effective input motions for surface and embedded foundations. This technique is applicable to a linear elastic system whose impulse response decreases to a small enough value before observing the reflected waves. The experimentally obtained impedance functions and effective input motions are compared with those obtained by the direct boundary integral equation method and the hybrid approach. Good agreement between the xperimental and analytical results validates the present method as well as the accuracy of the numerical tools.     

Sulfur isotope fractionation during bacterial sulfate reduction in organic-rich sediments

Isotope fractionation during sulfate reduction by natural populations of sulfate-reducing bacteria was investigated in the cyanobacterial microbial mats of Solar Lake, Sinai and the sediments of Logten Lagoon sulfuretum, Denmark. Fractionation was measured at different sediment depths, sulfate concentrations, and incubation temperatures. Rates of sulfate reduction varied between 0.1 and 37 micromoles cm-3 d-1, with the highest rates among the highest ever reported from natural sediments. The depletion of 34S during dissimilatory sulfate reduction ranged from 16% to 42%, with the largest 34S-depletions associated with the lowest rates of sulfate reduction and the lowest 34S-depletions with the highest rates. However, at high sulfate reduction rates (>10 micromoles cm-3 d-1) the lowest fractionation was 20% independent of the rates. Overall, there was a similarity between the fractionation obtained by the natural populations of sulfate reducers and previous measurements from pure cultures. This was somewhat surprising given the extremely high rates of sulfate reduction in the experiments. Our results are explained if we conclude that the fractionation was mainly controlled by the specific rate of sulfate reduction (mass cell-1 time-1) and not by the absolute rate (mass volume-1 time-1). Sedimentary sulfides (mainly FeS2) were on average 40% depleted in 34S compared to seawater sulfate. This amount of depletion was more than could be explained by the isotopic fractionations that we measured during bacterial sulfate reduction. Therefore, additional processes contributing to the fractionation of sulfur isotopes in the sediments are indicated. From both Solar Lake and Logten Lagoon we were able to enrich cultures of elemental sulfur-disproportionating bacteria. We suggest that isotope fractionation accompanying elemental sulfur disproportionation contributes to the 34S depletion of sedimentary sulfides at our study sites.     

Impact of wildfire on source water contributions in Devil Creek,CA: evidence from end‐member mixing analysis

A geochemical and end‐member mixing analysis (EMMA) is undertaken in Devil Canyon catchment, located in southern California, to further understanding of watershed behaviour and source water contributions after an acute and extensive wildfire. Physical and chemical transformations in post‐fire watersheds are known to increase overland flow and decrease infiltration, mainly due to formation of a hydrophobic layer at, or near, the soil surface. However, less is known about subsurface flow response in burned watersheds. The current study incorporates EMMA to evaluate and quantify source water contributions before, and after, a catchment affected by wildfires in southern California during the fall of 2003. Pre‐ and post‐fire stream water data were available at several sampling sites within the catchment, allowing the identification of contributing water sources at varying spatial scales. Proposed end‐member observations (groundwater, overland flow, shallow subsurface flow) were also collected to constrain and develop the catchment mixing model. Post‐fire source water changes are more evident in the smaller and faster responding sub‐basin (interior sampling point). Early post‐fire storm events are dominated by overland flow with no significant soil water or groundwater flow contribution. Inter‐storm streamwater in this smaller basin shows an increase in groundwater and a decrease in soil water. In the larger, baseflow‐dominated system, source water components appear less affected by fire. A slight increase in lateral flow is observed with only a slight decrease in baseflow. Changes in the post‐fire flow regimes affect nutrient loading and chemical response of the basin. Relatively rapid recovery of the chaparral ecosystem is evidenced, with active re‐growth and evapotranspiration evidenced by the fourth post‐fire rainy season. Copyright © 2008 John Wiley & Sons, Ltd.