Use of suppressive subtractive hybridization and cDNA arrays to discover patterns of altered gene expression in the liver of dihydrotestosterone and 11-ketotestosterone exposed adult male largemouth bass (Micropterus salmoides)

In this study male largemouth bass (LMB) were exposed to the naturally occurring androgens, dihydrotestosterone (DHT) or 11-ketotestosterone (11-KT) in order to identify genes that are differentially regulated by these steroid hormones. Using subtractive hybridization on livers of fish treated with DHT against vehicle control, many novel LMB genes were cloned. These genes were added to our gene library and arrayed. Six genes were up-regulated and five were down-regulated by both androgens. But, each androgen also regulated specific genes. One gene that was identified as a potential androgen marker was spermidine-spermine-N(1)-acetyltransferase that was up-regulated by both androgens. Determining which genes are responsive to natural androgens will help to identify biochemical pathways that are impacted.     

Adaptive balance of global development processes

We study the processes of global self-regulation of Earth’s biota (GAIA-theory) by applying the method of adaptive balance of causes proposed by the authors. By using, as an example, the Daisy World model constructed by this method, we reveal the phenomenon of preservation of the mean temperature of Earth’s surface due to the presence of the vegetation cover. We develop an integral model of global natural, social, and economic processes in which the World Ocean is one of the factors regulating the amount of greenhouse gases in the atmosphere. The decrease in the amount of greenhouse gases in the atmosphere caused by the GAIA-effects increases the number of hurricanes and floods on the Earth. As a result, the levels of ecological and social hazards for the mankind become much higher. To eliminate these threats, it is necessary to intensify the processes of self-organization of the society realized via the improvement of education, development of science, and global regulation of the competition for natural resources. We present the results of numerical experiments performed by using the model demonstrating possible scenarios of global development with regard for the processes of self-organization of the society. __________ Translated From Morskoi Gidrofizicheskii Zhurnal, No. 3, Pp. 62–80, May–June, 2007.     

Measurement of DNA single-strand breaks in gill and hemolymph cells of mussels, Mytilus sp., collected on the French Atlantic Coast

DNA single-strand breaks were measured by the comet assay in both gill and hemolymph cells of mussels collected in 3 sampling areas of the French coast (Pointe du Castelli, Pen Bron and Saint-Nazaire Harbour). Whole mussel tissue samples were also collected for the chemical determination of PAH, PCB and heavy metal concentrations. In mussel, a higher level of DNA strand breaks was measured in gill than in hemolymph cells (p < 0.01). Despite a factor of contamination from 2 to 3 between sites, no difference in the extent of mussel DNA strand breaks was shown between sampling locations (p > 0.05), questioning the sensitivity of the assays used in biomonitoring studies.     

The wadic project: A comprehensive field evaluation of directional wave instrumentation

The results of a comprehensive field trial of nearly all commercially available directional wave measurement systems at the Edda field in the North Sea during winter 1985-86 are presented. The results summarize the accuracy of the principal engineering wave parameters from each system and the dependence on sea state. Limiting factors on system performance and operational problems are also included in the assessment. Overall experience has been good with systems utilizing widely different measurement principles returning consistent results.     

On the possibility of forecasting the long-term air temperature variability that determines the hydrophysical structure and ecology of the Black Sea waters

Periods and amplitudes of long-term temperature fluctuations were obtained using the methods of spectral analysis and filtration of secular time series of the air temperature at 13 hydrometeorological stations in the Black Sea region. The prognostic calculations of the long-term air temperature variability are based on the results of processing of time series. The calculations of the air temperature agree with the data of observations. The possibility of the long-term air temperature variability prediction is shown.     

Forced Waves in a Bounded Basin with One Thermocline

Within the framework of the linear theory taking into account the action of the Coriolis force, we study waves induced in a continuously stratified fluid with one thermocline by periodic variations of pressure on the free surface. The fluid fills a bounded basin of constant depth. We establish the dependences of the wave amplitudes and wave velocities on the period of disturbing pressure. The sequence of transformations of generated waves from the barotropic character of motion to baroclinic depending on changes in the period of disturbing pressure is studied in detail.     

Cloning of the major 3-MC inducible phase I and phase II detoxification enzymes of plaice liver

cDNA's coding for cytochrome P4501A1 (CYP1A1), phenol UDP-glucuronosyltransferase (UDPGT) and glutathione S-transferase (GST-A) were cloned and sequenced from an expression library prepared from the liver of a 3-methylcholanthrene (3-MC) induced plaice. Plaice CYP1A1 and Phenol UDPGT display a high degree of structural conservation with homologous mammalian isoforms and their mRNAs were shown to be highly induced in liver after 3-MC treatment of fish. Expression of plaice GST-A, which displays closer homology to GSTs from plants and invertebrates than those of mammals, is repressed after 3-MC treatment.     

Investigation of internal waves generated by a diurnal barotropic tide in the vicinity of an oceanic ridge

Internal waves occurring near an oceanic ridge as a result of impingement of a barotropic tide are scrutinized in terms of the long theory for long waves. The ocean is assumed to be double-layered, the relief continuously changing, and the tidal wave on-running at an arbitrary angle. The dependences of the wave amplitudes and horizontal velocities on the ridge's latitude, the angle of tide running, and the depth of the density jump layer are obtained. Similar investigations for the model bottom relief have been conducted in refs 1 and 3.Translated by Vladimir A. Puchkin.