Immunological characterization of metallothionein in marine and freshwater fish

Metallothionein (MT) is a low-molecular-weight, cysteine-rich, metalbinding protein found in all vertebrates. The primary structure of the protein is evolutionary conserved, especially the location of cysteine-residues. There are nonetheless significant differences between MTs from different vertebrate classes, notably in major antigenic epitopes. Previous work has shown that MT from different fish species also differ. The objective of the present work was to characterize MT from temperate, sub-tropical and tropical fish species by their relative reactivities with two anti-fish MT antisera. One or both antisera cross-reacted with MT from all fish species investigated. Surprisingly, the relative reactivities of the two antisera with MT from species belonging to different taxonomic groups appeared to reflect differences at the level of family rather than differences between major phylogenetic lineages.     

Resource decline and adaptation through time: Fishers in San Miguel Bay, Philippines, 1980–1993

This article examines social conditions in a bay experiencing population growth, gear conflict, overfishing, and general resource decline. Sample surveys of fishing households carried out in 1980 and 1993 in nine villages of San Miguel Bay reveal patterns of continuity and change. The key continuity is sustained overall population growth in fishing villages. Among the key forms of change are those which demonstrate a degree of adaptation to resource decline: decreased participation in fishing; greater reliance of fishing households on nonfishing income; increased dependence on remittances of nonhousehold children; increased participation of women in nonhousehold labor; and dramatic growth in the number of fishing organizations involved in resource management. The findings suggest that resource management policies should be patterned after spontaneous adaptations to resource decline.     

Numerical experiments on the four-dimensional assimilation of the observation data derived in the Black Sea in June 1984 on the basis of a numerical energy-balanced model

A procedure for the four-dimensional (4D) analysis of the hydrophysical fields in the Black Sea with an assimilation of the temperature and salinity (T, S) data was realized on the basis of a numerical model which involves the primitive equations of motion, and the heat and salt advection equations. Two experiments were carried out which differed by the observation data assimilation procedure. Analysis has shown that the observation data assimilation procedure realized using the energy-balanced model allows the reproduction of some synoptic features of the circulation in the Black Sea. A comparison of two computations demonstrates the efficiency of assimilating the measurement data on the basis of the 4D analysis as compared with the sequential objective analysis.Translated by Mikhail M. Trufanov.     

Contaminant concentrations and toxicity of sea-surface microlayer near Los Angeles, California

Sea-surface microlayer samples were collected from six nearshore areas receiving different amounts of anthropogenic inputs. The samples were analyzed for selected trace metals, chlorinated hydrocarbons, and polycyclic aromatic hydrocarbons. The relative toxicities of the samples were determined with fish embryo bioassays.Contaminant concentrations generally increased from offshore to the inshore stations. Contaminant concentrations were several orders of magnitude higher in microlayer samples from the highly industrialized Los Angeles and Long Beach harbors compared to samples from a site 15km offshore. Microlayer samples from the inshore stations were significantly more toxic, and induced significantly more developmental abnormalities and chromosome aberrations, than samples from the offshore stations.     

钝顶螺旋藻部分原生质体及单细胞的制备与培养

于1992年1月-1994年4月，进行超声波处理方法制备钝顶螺旋藻部分原生质体以作为基因工程受体，以及制备单细胞用于固体平板克隆化培养的研究。研究结果表明：超声波以20kHz频率、15W功率作用30s，可使藻丝体断裂成15．0±1．6个细胞长度；延长作用时间，至2－6个细胞长度时，细胞壁结构遭到破坏，形成部分原生质体；继续作用，可形成少量原生质体和大量单细胞。断裂藻丝体、部分原生质体、单细胞以及原生质体均可涂布于固体培养基上再生或生长。以一定密度涂布单细胞与原生质体，能够形成彼此分开的单个克隆，可用于筛选及遗传分析。本文提供了一种节省溶菌酶的制备螺旋藻透性体的方法，超声波作用利于外源基因的导入，而涂布培养利于进一步的筛选和形成克隆。     

Influences of wave stretching on the response of wave-excited offshore platforms

Critical examinations are reported for the effects of wave stretching on realistic representations of the wave forces that act on offshore structures. Examinations are also made for the importance of such effects on the corresponding structural responses, and it is demonstrated that the effects of stretching on the governing wave forces and the resulting structural responses are small, indicating that they can be ignored in design practice. Valuable insight is further provided into the definition of the wave excitation, and physical interpretations are made which show that the actions of stretching cannot materially influence the governing excitation and the corresponding structural response.     

黄河口及渤海中南部沉积物工程特性及其机理

采用Ｋａｓｔｅｎ取样器从黄河口及渤海湾中南部海底沉积物中取样，对样品通过土工性质、微结构研究以及粘土矿物分析，阐明本研究区三维空间展布的海底沉积物形成机理及其工程地质规律，论证水动力作用和斜坡不稳定性对工程特性和微结构的重要影响．