Faecal-indicator bacteria and sedimentary processes in estuarine mudflats (Seine, France)

Over a three-year period, quantification of faecal indicators and the molecular detection of Escherichia coli and Salmonella were monitored in sediments from three contrasting mudflats of the Seine estuary (France). The elevation of the mudflat surface was monitored concurrently using a high-resolution altimeter. During the period of the study, estuarine mudflats were areas of deposition for faecal-indicator bacteria and were mainly controlled by sedimentary processes. In the intertidal freshwater and subtidal mudflats, the highest abundances of faecal-indicator bacteria were counted during a depositional period. Maximum levels were observed in the freshwater mudflats during periods of high flow: thermotolerant coliforms: 3.9 x 10(4) cfu cm(-2), enterococci: 1.2 x 10(4) cfu cm(-2), Clostridium perfringens spores: 9.8 x 10(5) spores cm(-2). Loss of culturability of enteric bacteria in sediment microcosms demonstrated the remediatory capacity of the mudflats, even if they might be a secondary source of bacteria-forming spores to the water column through erosion and resuspension events.     

Nutrient availability in support of Karenia brevis blooms on the central West Florida Shelf: What keeps Karenia blooming?

Identifying nutrient sources, primarily nitrogen (N) and phosphorus (P), sufficient to support high biomass blooms of the red tide dinoflagellate, Karenia brevis, has remained problematic. The West Florida Shelf is oligotrophic, yet populations >10⁶ cells L⁻¹ frequently occur and blooms can persist for months. Here we examine the magnitude and variety of sources for N and P that are available to support blooms. Annual average in situ or background concentrations of inorganic N in the region where blooms occur range 0.02–0.2 μM while inorganic P ranges 0.025–0.24 μM. Such concentrations would be sufficient to support the growth of populations up to ∼3×10⁴ cells L⁻¹ with at least a 1 d turnover rate. Organic N concentrations average 1–2 orders of magnitude greater than inorganic N, 8–14 μM while organic P concentrations average 0.2–0.5 μM. Concentrations of organic N are sufficient to support blooms >10⁵ cells L⁻¹ but the extent to which this complex mixture of N species is utilizable is unknown. Other sources of nutrients included in our analysis are aerial deposition, estuarine flux, benthic flux, zooplankton excretion, N₂-fixation, and subsequent release of organic and inorganic N by Trichodesmium spp., and release of N and P from dead and decaying fish killed by the blooms. Inputs based on atmospheric deposition, benthic flux, and N₂-fixation, were minor contributors to the flux required to support growth of populations >2.6×10⁴ cells L⁻¹. N and P from decaying fish could theoretically maintain populations at moderate concentrations but insufficient data on the flux and subsequent mixing rates does not allow us to calculate average values. Zooplankton excretion rates, based on measured zooplankton population estimates and excretion rates could also supply all of the N and P required to support populations of 10⁵ and 10⁶ cells L⁻¹, respectively, but excretion is considered as “regenerated” nutrient input and can only maintain biomass rather than contribute to “new” biomass. The combined estuarine flux from Tampa Bay, Charlotte Harbor, and the Caloosahatchee River can supply a varying, but at times significant level of N and P to meet growth and photosynthesis requirements for populations of approximately 10⁵ cells L⁻¹ or below. Estimates of remineralization of dead fish could supply a significant proportion of bloom maintenance requirements but the rate of supply must still be determined. Overall, a combination of sources is required to maintain populations >10⁶ cells L⁻¹.     

Thallium Mass Fraction and Stable Isotope Ratios of Sixteen Geological Reference Materials

Thallium stable isotope ratio and mass fraction measurements were performed on sixteen geological reference materials spanning three orders of magnitude in thallium mass fraction, including both whole rock and partially separated mineral powders. For stable isotope ratio measurements, a minimum of three independent digestions of each reference material was obtained. High‐precision trace element measurements (including Tl) were also performed for the majority of these RMs. The range of Tl mass fractions represented is 10 ng g^?1 to 16 μg g^?1, and Tl stable isotope ratios (reported for historical reasons as ε²⁰⁵Tl relative to NIST SRM 997) span the range ?4 to +2. With the exception – attributed to between‐bottle heterogeneity – of G‐2, the majority of data are in good agreement with published or certified values, where available. The precision of mean of independent measurement results between independent dissolutions suggests that, for the majority of materials analysed, a minimum digested mass of 100 mg is recommended to mitigate the impact of small‐scale powder heterogeneity. Of the sixteen materials analysed, we therefore recommend for use as Tl reference materials the USGS materials BCR‐2, COQ‐1, GSP‐2 and STM‐1; CRPG materials AL‐I, AN‐G, FK‐N, ISH‐G, MDO‐G, Mica‐Fe, Mica‐Mg and UB‐N; NIST SRM 607 and OREAS14P.     

An Inter-Laboratory Assessment of the Thorium Isotopic Composition of Synthetic and Rock Reference Materials

We present a concerted international effort to cross-calibrate five synthetic Th isotope reference materials (UCSC Th "A", OU Th "U", WUN, IRMM-35 and IRMM-36), and six rock reference materials (UCSC TML, Icelandic ATHO, USGS BCR-2, USGS W-2, USGS BHVO-2, LV18) using multi-collector inductively coupled plasma-mass spectrometry (MC-ICP-MS). We then compare our new values with a compilation of literature mass spectrometric data for these reference materials and derive recommended "consensus"²³⁰Th/²³²Th values for each. We also present isotope dilution U and Th concentration data for four rock reference materials (UCSC TML, Icelandic ATHO, USGS BCR-2, USGS W-2).     

Going to the Extremes

Projections of changes in climate extremes are critical to assessing the potential impacts of climate change on human and natural systems. Modeling advances now provide the opportunity of utilizing global general circulation models (GCMs) for projections of extreme temperature and precipitation indicators. We analyze historical and future simulations of ten such indicators as derived from an ensemble of 9 GCMs contributing to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change (IPCC-AR4), under a range of emissions scenarios. Our focus is on the consensus from the GCM ensemble, in terms of direction and significance of the changes, at the global average and geographical scale. The climate extremes described by the ten indices range from heat-wave frequency to frost-day occurrence, from dry-spell length to heavy rainfall amounts. Historical trends generally agree with previous observational studies, providing a basic sense of reliability for the GCM simulations. Individual model projections for the 21st century across the three scenarios examined are in agreement in showing greater temperature extremes consistent with a warmer climate. For any specific temperature index, minor differences appear in the spatial distribution of the changes across models and across scenarios, while substantial differences appear in the relative magnitude of the trends under different emissions rates. Depictions of a wetter world and greater precipitation intensity emerge unequivocally in the global averages of most of the precipitation indices. However, consensus and significance are less strong when regional patterns are considered. This analysis provides a first overview of projected changes in climate extremes from the IPCC-AR4 model ensemble, and has significant implications with regard to climate projections for impact assessments. An erratum to this article is available at . An erratum to this article can be found at     

Ancient geochemical cycling in the Earth as inferred from Fe isotope studies of banded iron formations from the Transvaal Craton

Variations in the isotopic composition of Fe in Late Archean to Early Proterozoic Banded Iron Formations (BIFs) from the Transvaal Supergroup, South Africa, span nearly the entire range yet measured on Earth, from –2.5 to +1.0‰ in ⁵⁶Fe/⁵⁴Fe ratios relative to the bulk Earth. With a current state-of-the-art precision of ±0.05‰ for the ⁵⁶Fe/⁵⁴Fe ratio, this range is 70 times analytical error, demonstrating that significant Fe isotope variations can be preserved in ancient rocks. Significant variation in Fe isotope compositions of rocks and minerals appears to be restricted to chemically precipitated sediments, and the range measured for BIFs stands in marked contrast to the isotopic homogeneity of igneous rocks, which have δ⁵⁶Fe=0.00±0.05‰, as well as the majority of modern loess, aerosols, riverine loads, marine sediments, and Proterozoic shales. The Fe isotope compositions of hematite, magnetite, Fe carbonate, and pyrite measured in BIFs appears to reflect a combination of (1) mineral-specific equilibrium isotope fractionation, (2) variations in the isotope compositions of the fluids from which they were precipitated, and (3) the effects of metabolic processing of Fe by bacteria. For minerals that may have been in isotopic equilibrium during initial precipitation or early diagenesis, the relative order of δ⁵⁶Fe values appears to decrease in the order magnetite > siderite > ankerite, similar to that estimated from spectroscopic data, although the measured isotopic differences are much smaller than those predicted at low temperature. In combination with on-going experimental determinations of equilibrium Fe isotope fractionation factors, the data for BIF minerals place additional constraints on the equilibrium Fe isotope fractionation factors for the system Fe(III)–Fe(II)–hematite–magnetite–Fe carbonate. δ⁵⁶Fe values for pyrite are the lowest yet measured for natural minerals, and stand in marked contrast to the high δ⁵⁶Fe values that are predicted from spectroscopic data. Some samples contain hematite and magnetite and have positive δ⁵⁶Fe values; these seem best explained through production of high ⁵⁶Fe/⁵⁴Fe reservoirs by photosynthetic Fe oxidation. It is not yet clear if the low δ⁵⁶Fe values measured for some oxides, as well as Fe carbonates, reflect biologic processes, or inorganic precipitation from low-δ⁵⁶Fe ferrous-Fe-rich fluids. However, the present results demonstrate the great potential for Fe isotopes in tracing the geochemical cycling of Fe, and highlight the need for an extensive experimental program for determining equilibrium Fe isotope fractionation factors for minerals and fluids that are pertinent to sedimentary environments.     

Oceanographic and climatic implications of the Palaeocene carbon isotope maximum

We have compared detailed planktonic and benthonic foraminiferal carbon and oxygen isotope records from the Palaeocene and early Eocene successions at DSDP Site 577 (Shatsky Rise, North Pacific), a composite section derived from DSDP Leg 74 sites (Walvis Ridge, South Atlantic) and a composite section from ODP Leg 113 sites (Maud Rise, Weddell Sea). The δ¹³C records of Palaeocene and early Eocene Foraminifera at Site 577 and the Leg 74 sites show that an increase in δ¹³C values in surface waters at 64 Ma (end of Zone P1) resulted in increased vertical carbon isotope gradients (δ¹³C) between surface and deeper dwelling planktonic foraminifera, and between surface-dwelling planktonics and benthonic foraminifera which became progressively steeper until the iniddle Late Palaeocene (Zone P4). This steepening also occurs in the latest Palaeocene of the composite Leg 113 section and can be explained by an increase in surface ocean productivity. This increase in productivity probably resulted in an expansion of the oxygen minimum zone (OMZ). Benthonic δ¹³C values increased during the late Palaeocene in Site 577 and the composite Leg 74 section, suggesting that the Palaeocene carbon isotope maximum was composed of both within-ocean reservoir (increased surface water productivity) and between-reservoir (organic carbon burial) ftactionation effects. The benthonic δ¹³C increase lags the surface ocean δ¹³C increase in the early Palaeocene (63–64 Ma) suggesting that surface water productivity increase probably led an increase in the burial rate of organic carbon relative to carbonate sedimentation. Moreover, inter-site δ¹³C comparisons suggest that the locus of deep to intermediate water formation for the majority of the Palaeocene and the earliest Eocene was more likely to have been in the high southern latitudes than in the lower latitudes. Oxygen isotope data show a decline in deeper water temperatures in the early and early late Palaeocene, followed by a temperature increase in the late Palaeocene and across the PalaeoceneEocene boundary. We speculate that these changes in deeper water temperatures were related to the flux of CO₂ between the oceans and the atmosphere through a mechanism operating at the high southern latitudes.