Microbial reduction of chromium from the hexavalent to divalent state

We demonstrate that Shewanella oneidensis, a metal-reducing bacteria species with cytoplasmic-membrane-bound reductases and remarkably diverse respiratory capabilities, reduced Cr(VI) to Cr(II) in anaerobic cultures where chromate was the sole terminal electron acceptor. Individual cell microanalysis by transmission electron microscopy (TEM) using electron energy-loss spectroscopy (EELS) and energy dispersive X-ray spectroscopy (EDXS) demonstrates Cr(II) concentrated near the cytoplasmic membrane, suggesting the terminal reduction pathway is intracellularly localized. Further, estimated cellular Cr(II) concentrations are relatively high at upwards of 0.03-0.09 g Cr/g bacterium. Accumulation of Cr(II) is observed in S. oneidensis cells prior to the formation of submicron-sized precipitates of insoluble Cr(III) on their surfaces. Furthermore, under anaerobic conditions, Cr(III) precipitates that encrust cells are shown to contain Cr(II) that is likely bound in the net negatively charged extracellular biopolymers which can permeate the surfaces of the precipitates. In otherwise nearly identical incubations, Cr(III) precipitate formation was observed in cultures maintained anaerobic with bubbled nitrogen but not in three replicate cultures in an anaerobic chamber.     

The subsystem of open clusters in the post-hipparcos era: Cluster structural parameters and proper motions

The wide neighborhoods of 401 open clusters are analyzed using the modern, high-precision, homogeneous ASCC-2.5 all-sky catalog. More than 28000 possible cluster members (including about 12500 most probable members) are identified using kinematic and photometric criteria. Star counts with the ASCC-2.5 and USNO-A2.0 catalogs are used to determine the angular and linear radii of the cluster cores and coronas, which exceed the previously published values by factors of two to three. The segregation (differing central concentration) of member stars by magnitude is observed. The mean proper motions are determined directly in the Hipparcos system for 401 clusters, for 183 of them for the first time. The heliocentric distances of 118 clusters are determined for the first time based on color-magnitude diagrams for their identified members.     

An Attempt to Partition Stomatal and Non-stomatal Ozone Deposition Parts on a Short Grassland

To evaluate the damaging effect of tropospheric ozone on vegetation, it is important to evaluate the stomatal uptake of ozone. Although the stomatal flux is a dominant pathway of ozone deposition onto vegetated surfaces, non-stomatal uptake mechanisms such as soil and cuticular deposition also play a vital role, especially when the leaf area index \({LAI}< 4\). In this study, we partitioned the canopy conductance into stomatal and non-stomatal components. To calculate the stomatal conductance of water vapour for sparse vegetation, we firstly partitioned the latent heat flux into effects of transpiration and evaporation using the Shuttleworth–Wallace (SW) model. We then derived the stomatal conductance of ozone using the Penman–Monteith (PM) theory based on the similarity to water vapour conductance. The non-stomatal conductance was calculated by subtracting the stomatal conductance from the canopy conductance derived from directly-measured fluxes. Our results show that for short vegetation (LAI \(=\) 0.25) dry deposition of ozone was dominated by the non-stomatal flux, which exceeded the stomatal flux even during the daytime. At night the stomatal uptake of ozone was found to be negligibly small. In the case of vegetation with \({LAI}\approx 1\), the daytime stomatal and non-stomatal fluxes were of the same order of magnitude. These results emphasize that non-stomatal processes must be considered even in the case of well-developed vegetation where cuticular uptake is comparable in magnitude with stomatal uptake, and especially in the case of vegetated surfaces with \({LAI}< 4\) where soil uptake also has a role in ozone deposition.     

Exploration for scheelite in East Greenland — A case study

An intensive scheelite exploration program was carried out in Precambrian crystalline and sedimentary rocks intruded by granites of Precambrian and Caledonian ages in East Greenland (70–74°30′N). Previous heavy-mineral panning (2100 samples within an area of 100,000 km²) formed the basis for selection of scheelite-anomalous subregions (1550 km²)In the subregions, pan-concentrate samples were taken from first- and second-order rivers and from mid and side moraines of active glaciers. All samples were studied in the field under UV light, and scheelite grains were counted. A consideration of the distribution of scheelite in the samples together with the river and glacial drainage systems, led to definition of the potential source areas of the scheelite within localities of 2–5 km².Within the localities, panning of scree fines (samples every 100–200 m along talus slopes) and UV-light traverses at night led to the finding of outcropping or sub-outcropping scheelite mineralisation. Scheelite was observed associated with granite-carbonate contact zones, quartz vein stockworks, and fault zones in limestones, at nine localities within the 300-km-long zone of investigation.The heavy-mineral panning method with the counting of scheelite grains in the field and the subsequent definition of potential scheelite-bearing areas has the advantage that it is possible to execute a program from the subregional to the outcropping mineralisation stage in one field season. The investigation in this case was performed by five geologists during the 1979 2.5-month field season.     

Constraints from melt inclusions and their host olivines on the petrogenesis of Oligocene-Early Miocene Xindian basalts, Chifeng area, North China Craton

The geochemical characteristics of melt inclusions and their host olivines provide important information on the processes that create magmas and the nature of their mantle and crustal source regions. We report chemical compositions of melt inclusions, their host olivines and bulk rocks of Xindian basalts in Chifeng area, North China Craton. Compositions of both bulk rocks and melt inclusions are tholeiitic. Based on petrographic observations and compositional variation of melt inclusions, the crystallizing sequence of Xindian basalts is as follows: olivine (at MgO > ~5.5 wt%), plagioclase (beginning at MgO = ~5.5 wt%), clinopyroxene and ilmenite (at MgO < 5.0 wt%). High Ni contents and Fe/Mn ratios, and low Ca and Mn contents in olivine phenocrysts, combining with low CaO contents of relatively high MgO melt inclusions (MgO > 6 wt%), indicate that Xindian basalts are possibly derived from a pyroxenite source rather than a peridotite source. In the CS-MS-A diagram, all the high MgO melt inclusions (MgO > 6.0 wt%) project in the field between garnet + clinopyroxene + liquid and garnet + clinopyroxene + orthopyroxene + liquid near 3.0 GPa, further suggesting that residual minerals are mainly garnet and clinopyroxene, with possible presence of orthopyroxene, but without olivine. Modeling calculations using MELTS show that the water content of Xindian basalts is 0.3–0.7 wt% at MgO = 8.13 wt%. Using 20–25 % of partial melting estimated by moderately incompatible element ratios, the water content in the source of Xindian basalts is inferred to be ≥450 ppm, much higher than 6–85 ppm in dry lithospheric mantle. The melting depth is inferred to be ~3.0 GPa, much deeper than that of tholeiitic lavas (<2.0 GPa), assuming a peridotite source with a normal mantle potential temperature. Such melting depth is virtually equal to the thickness of lithosphere beneath Chifeng area (~100 km), suggesting that Xindian basalts are derived from the asthenospheric mantle, if the lithospheric lid effect model is assumed.