Copper isotope fractionation during surface adsorption and intracellular incorporation by bacteria

Copper isotopes may prove to be a useful tool for investigating bacteria-metal interactions recorded in natural waters, soils, and rocks. However, experimental data which attempt to constrain Cu isotope fractionation in biologic systems are limited and unclear. In this study, we utilized Cu isotopes (δ⁶⁵Cu) to investigate Cu-bacteria interactions, including surface adsorption and intracellular incorporation. Experiments were conducted with individual representative species of Gram-positive (Bacillus subtilis) and Gram-negative (Escherichia coli) bacteria, as well as with wild-type consortia of microorganisms from several natural environments. Ph-dependent adsorption experiments were conducted with live and dead cells over the pH range 2.5-6. Surface adsorption experiments of Cu onto live bacterial cells resulted in apparent separation factors (Δ⁶⁵Cu_{solution-solid} = δ⁶⁵Cu_solution − δ⁶⁵Cu_solid) ranging from +0.3‰ to +1.4‰ for B. subtilis and +0.2‰ to +2.6‰ for E. coli. However, because heat-killed bacterial cells did not exhibit this behavior, the preference of the lighter Cu isotope by the cells is probably not related to reversible surface adsorption, but instead is a metabolically-driven phenomenon. Adsorption experiments with heat-killed cells yielded apparent separation factors ranging from +0.3‰ to −0.69‰ which likely reflects fractionation from complexation with organic acid surface functional group sites. For intracellular incorporation experiments the lab strains and natural consortia preferentially incorporated the lighter Cu isotope with an apparent Δ⁶⁵Cu_{solution-solid} ranging from ∼+1.0‰ to +4.4‰. Our results indicate that live bacterial cells preferentially sequester the lighter Cu isotope regardless of the experimental conditions. The fractionation mechanisms involved are likely related to active cellular transport and regulation, including the reduction of Cu(II) to Cu(I). Because similar intracellular Cu machinery is shared by fungi, plants, and higher organisms, the influence of biological processes on the δ⁶⁵Cu of natural waters and soils is probably considerable.     

A review of undulated sediment features on Mediterranean prodeltas: distinguishing sediment transport structures from sediment deformation

Most Mediterranean prodeltas show undulated sediment features on the foresets of their Holocene wedges. These features have been described all along the Mediterranean for the last 30 years and interpreted as either soft sediment deformation and incipient landsliding, and more recently, as sediment transport structures. We perform a review and detailed analysis of these undulated sediment features using ultrahigh-resolution seismic and bathymetric data as well as geotechnical information and hydrodynamic time series and hydrographic transects. In this study we show that the characteristics of the sediment undulations (configuration of the reflections down section and between adjacent undulations and overall morphologic characteristics) are incompatible with a genesis by sediment deformation alone and do not show evidence of sediment deformation in most cases. Various processes in the benthic boundary layer can be invoked to explain the variety of features observed in the numerous areas displaying sediment undulations.     

Tumbi Landslide, Papua New Guinea: rainfall induced?

On 24 January 2012, a fatal landslide with an estimated volume of 3 Mm³ hit villagers and infrastructure in the Tagali Valley, Southern Highlands Province, Papua New Guinea (PNG). Although a moderate event by PNG standards, the associated human casualties and infrastructure destruction give a human as well as a scientific need to review the potential causes for the event. PNG experiences numerous landslides annually, most of which are trigged either by rainfall events or seismic activity. Here, we review the short-term rainfall patterns leading up to the slope failure at Tumbi Quarry and compare the rainfall accumulations obtained over durations of 30, 60 and 90 days prior to the landslide, with comparable rainfall accumulations obtained for other failures observed in PNG over a 12-year period. Additionally, a review of long-term rainfall over a period of 6 months prior to the failure is completed as well as an assessment of seismic activity. Based on our analysis, we believe a seismic trigger to be very unlikely, while the increase in rainfall in the 2 weeks prior to the failure in conjunction with a high-intensity rainfall event at the end of October 2011 could have had a greater influence in enhancing rather than restraining slope failure. This, in addition to natural denudational processes, geological structure and the anthropogenic activity in the vicinity of the landslide, could all have served to affect slope stability.     

Influence of ambient flow around the horseshoe crabLimulus polyphemus on the distribution and orientation of selected epizoans

Cumulatively scored distributions of the slipper shell gastropodCrepidula sp., the sand-building polychaete wormSabellaria vulgaris, the calcareous meandering tubed polychaeteFilograna implexa, the barnacleSemibalanaus balanoides, and the lacy bryozoanSchizoporella sp. were mapped on a cm-square gridded silhouette of the prosoma, opisthosoma, and movable spines, and telson of the horseshoe crabLimulus polyphemus stranded on Reeds Beach, Cape May County, New Jersey in the Delaware Bay. Statistically tested and contoured epizoan frequencies for 81 males and 59 females revealed that slipper shells and agglutinated worm tubes are concentrated near the movable spines on the opisthosoma, but rare on recessed chilarial or opercular pleurites, the prosoma, and telson. Barnacles are disproportionately concentrated in entapophyseal pits on the opisthosoma and in the longitudinal furrows flanking the cardiac lobe of the prosoma. Bryozoans are concentrated near movable marginal spines of the opisthosoma, but also found on all areas of the prosoma, opisthosoma, and hinge area in between, as well as the telson. Different morphologic sites for their respective modal concentrations may result from competitive exclusion of bryozoans by barnacles. Genal angles have lower statistically predicted frequencies of bryozoans, barnacles, and sand worm tubes. Apertural openings of calcareous tubes are predominantly posteriorly oriented whereas the anterior magin of slipper shells are randomly oriented on the opisthosoma. Eschewing differential mortality, nonrandom distributions indicate possible: rugophilic (groove-seeking) larval settlement behavior for barnacles that preferred the recessed hinge area, longitudinal grooves, and pits; rheophilic (current-seeking) larval settlement behavior for the gastropods and tube-building polychaetes that filter feed in the eddies generated near the movable spines of the downcurrent-sloping opisthosoma; and geotactic (gravity-influenced) larval settlement or post-settlement migration to the vaulted crest (cardiac lobe). Deficiencies of protruding epizoic skeletons on the genal angles and anterior margin of the host may be due to removal by abrasion as the horseshoe crab plowed through sediment or shearing of the epizoans from the carapace substratum in strong currents or waves.     

Geology of the Victor Kimberlite, Attawapiskat, Northern Ontario, Canada: cross-cutting and nested craters

The pipe shapes, infill and emplacement processes of the Attawapiskat kimberlites, including Victor, contrast with most of the southern African kimberlite pipes. The Attawapiskat kimberlite pipes are formed by an overall two-stage process of (1) pipe excavation without the development of a diatreme (sensu stricto) and (2) subsequent pipe infilling. The Victor kimberlite comprises two adjacent but separate pipes, Victor South and Victor North. The pipes are infilled with two contrasting textural types of kimberlite: pyroclastic and hypabyssal-like kimberlite. Victor South and much of Victor North are composed of pyroclastic spinel carbonate kimberlites, the main features of which are similar: clast-supported, discrete macrocrystal and phenocrystal olivine grains, pyroclastic juvenile lapilli, mantle-derived xenocrysts and minor country rock xenoliths are set in serpentine and carbonate matrices. These partly bedded, juvenile lapilli-bearing olivine tuffs appear to have been formed by subaerial fire-fountaining airfall processes.

The Victor South pipe has a simple bowl-like shape that flares from just below the basal sandstone of the sediments that overlie the basement. The sandstone is a known aquifer, suggesting that the crater excavation process was possibly phreatomagmatic. In contrast, the pipe shape and internal geology of Victor North are more complex. The northwestern part of the pipe is dominated by dark competent rocks, which resemble fresh hypabyssal kimberlite, but have unusual textures and are closely associated with pyroclastic juvenile lapilli tuffs and country rock breccias±volcaniclastic kimberlite. Current evidence suggests that the hypabyssal-like kimberlite is, in fact, not intrusive and that the northwestern part of Victor North represents an early-formed crater infilled with contrasting extrusive kimberlites and associated breccias. The remaining, main part of Victor North consists of two macroscopically similar, but petrographically distinct, pyroclastic kimberlites that have contrasting macrodiamond sample grades. The juvenile lapilli of each pyroclastic kimberlite can be distinguished only microscopically. The nature and relative modal proportion of primary olivine phenocrysts in the juvenile lapilli are different, indicating that they derive from different magma pulses, or phases of kimberlite, and thus represent separate eruptions. The initial excavation of a crater cross-cutting the earlier northwestern crater was followed by emplacement of phase (i), a low-grade olivine phenocryst-rich pyroclastic kimberlite, and the subsequent eruption of phase (ii), a high-grade olivine phenocryst-poor pyroclastic kimberlite, as two separate vents nested within the original phase (i) crater. The second eruption was accompanied by the formation of an intermediate mixed zone with moderate grade. Thus, the final pyroclastic pipe infill of the main part of the Victor North pipe appears to consist of at least three geological/macrodiamond grade zones.

In conclusion, the Victor kimberlite was formed by several eruptive events resulting in adjacent and cross-cutting craters that were infilled with either pyroclastic kimberlite or hypabyssal-like kimberlite, which is now interpreted to be of probable extrusive origin. Within the pyroclastic kimberlites of Victor North, there are two nested vents, a feature seldom documented in kimberlites elsewhere. This study highlights the meaningful role of kimberlite petrography in the evaluation of diamond deposits and provides further insight into kimberlite emplacement and volcanism.     

The ecological implications of a Yakutian mammoth's last meal

Part of a large male woolly mammoth (Mammuthus primigenius) was preserved in permafrost in northern Yakutia. It was radiocarbon dated to ca. 18,500 ¹⁴C yr BP (ca. 22,500 cal yr BP). Dung from the lower intestine was subjected to a multiproxy array of microscopic, chemical, and molecular techniques to reconstruct the diet, the season of death, and the paleoenvironment. Pollen and plant macro-remains showed that grasses and sedges were the main food, with considerable amounts of dwarf willow twigs and a variety of herbs and mosses. Analyses of 110-bp fragments of the plastid rbcL gene amplified from DNA and of organic compounds supplemented the microscopic identifications. Fruit-bodies of dung-inhabiting Ascomycete fungi which develop after at least one week of exposure to air were found inside the intestine. Therefore the mammoth had eaten dung. It was probably mammoth dung as no bile acids were detected among the fecal biomarkers analysed. The plant assemblage and the presence of the first spring vessels of terminal tree-rings of dwarf willows indicated that the animal died in early spring. The mammoth lived in extensive cold treeless grassland vegetation interspersed with wetter, more productive meadows. The study demonstrated the paleoecological potential of several biochemical analytical techniques.     

Regional magma plumbing and emplacement mechanisms of the Faroe‐Shetland Sill Complex: implications for magma transport and petroleum systems within sedimentary basins

The movement of magma through the shallow crust and the impact of subsurface sill complexes on the hydrocarbon systems of prospective sedimentary basins has long been an area of interest and debate. Based on 3D seismic reflection and well data, we present a regional analysis of the emplacement and magmatic plumbing system of the Palaeogene Faroe‐Shetland Sill Complex (FSSC), which is intruded into the Mesozoic and Cenozoic sequences of the Faroe‐Shetland Basin (FSB). Identification of magma flow directions through detailed seismic interpretation of approximately 100 sills indicates that the main magma input zones into the FSB were controlled primarily by the NE–SW basin structure that compartmentalise the FSB into its constituent sub‐basins. An analysis of well data shows that potentially up to 88% of sills in the FSSC are <40 m in thickness, and thus below the vertical resolution limit of seismic data at depths at which most sills occur. This resolution limitation suggests that caution needs to be exercised when interpreting magmatic systems from seismic data alone, as a large amount of intrusive material could potentially be missed. The interaction of the FSSC with the petroleum systems of the FSB is not well understood. Given the close association between the FSSC and potential petroleum migration routes into some of the oil/gas fields (e.g. Tormore), the role the intrusions may have played in compartmentalisation of basin fill needs to be taken fully into account to further unlock the future petroleum potential of the FSB.     

Seismic stratigraphy of the Adare Trough area, Antarctica

The Adare Trough, located 100 km NE of Cape Adare, Antarctica, is the extinct third arm of a Tertiary spreading ridge that separated East from West Antarctica. We use seismic reflection data, tied to DSDP Site 274, to link our seismic stratigraphic interpretation to changes in ocean-bottom currents, Ross Sea ice cover, and regional tectonics through time. Two extended unconformities are observed in the seismic profiles. We suggest that the earliest hiatus (early Oligocene to Mid-Miocene) is related to low sediment supply from the adjacent Ross Shelf, comprised of small, isolated basins. The later hiatus (mid-Miocene to late Miocene) is likely caused by strong bottom currents sourced from the open-marine Ross Sea due to increased Antarctic glaciation induced by mid-Miocene cooling (from Mi-3). Further global cooling during the Pliocene, causing changes in global ocean circulation patterns, correlates with Adare Basin sediments and indicate the continuing but weakened influence of bottom currents. The contourite/turbidite pattern present in the Adare Trough seismic data is consistent with the 3-phase contourite growth system proposed for the Weddell Sea and Antarctic Peninsula. Multibeam bathymetry and seismic reflection profiles show ubiquitous volcanic cones and intrusions throughout the Adare Basin that we interpret to have formed from the Oligocene to the present. Seismic reflection profiles reveal trans-tensional/strike-slip faults that indicate oblique extension dominated Adare Trough tectonics at 32–15 Ma. Observed volcanism patterns and anomalously shallow basement depth in the Adare Trough area are most likely caused by mantle upwelling, an explanation supported by mantle density reconstructions, which show anomalously hot mantle beneath the Adare Trough area forming in the Late Tertiary.