Purification and Characterization of a Novel Hydrolase That Can Specifically Degrade the Polysaccharide Isolated from Green Seaweed Ulva prolifera

The extracellular polysaccharide hydrolase-producing strain EP-1 was isolated from seawater and identified as Paenibacillus pabuli. Furthermore, a homogeneous extracellular polysaccharide hydrolase from Paenibacillus pabuli EP-1 was purified by combining ion-exchange chromatography and size exclusion chromatography with a purification fold of 90.69 and recovery of 16.23%. Characterization of the purified polysaccharide hydrolase revealed a molecular mass of 38 k Da and optimum activity at 45℃ and pH 6.0. The polysaccharide hydrolase maintained its stability within a wide range of pH(3.0–12.0) and thermal stability when the temperature was below 50℃. The presence of Hg²⁺, Fe²⁺, Mn²⁺, Co²⁺ and SDS notably decreased hydrolase activity, and organic solvents such as formaldehyde, acetone, DMF and acetonitrile completely inhibited hydrolase activity. The purified hydrolase had no activity on agar, carrageenan, gellan gum, sodium alginate, or starch, but effectively hydrolyzed the polysaccharide from Ulva prolifera. The Km and Vmax values of this hydrolase were 43.84 mg m L^-1 and 4.33 mg m L^-1 min^-1, respectively. The sequence analysis with quantitative time-of-flight mass spectrometry indicated that the hydrolase was an endoglucanase.     

流域尺度土地利用调蓄视角的雨洪管理探析

发达国家雨洪管理焦点历经管网设计、不透水面阈值控制、低影响开发及土地利用管理等过程,呈现"多尺度多视角衔接"趋势。本文聚焦太湖流域土地利用调蓄功能变化机制及管理应用,揭示了1985-2015年来建设占用耕地是调蓄功能降低的主要原因;发现基于小流域的调蓄功能变化与建设用地强度整体低关联,但局部高相关,高相关的地区位于大城市或某些乡镇周边的建设增长热区,而这些热区的建设用地与调蓄空间规模较大且接触机会更多,是开发之前调蓄空间保护、开发控制以及开发之后综合管理等3个土地利用调蓄目标因子协调不当的结果;最后提出流域尺度土地利用调蓄视角的雨洪管理"345"模式,即以3个目标因子和5类控制要素为基础,实施土地利用调蓄创建、防御、拓展与保护等4类差别化战略模式,从更大尺度认识老城区和新区的雨洪关联,拓展海绵城市建设的本土认知和视野。     

城市居民压力源对幸福感的影响研究——基于乡村旅游休闲参与的角度

居民幸福感研究日益受到重视,以往研究多是从自然环境、社会人文环境和城市化进程等方面探讨大尺度空间环境因素对城市居民幸福感的影响。结合乡村旅游的角度探讨城市居民幸福感的影响研究,目前还较少。运用SEM分析方法,探究处于多维压力之下的城市居民旅游者如何通过前往乡村进行旅游休闲活动提升幸福感,进行模型构建与分析,结果显示：① 压力源对调适策略有显著正向影响,对休闲参与不显著,但不同群体影响差异显著,压力调适对休闲参与有显著正向影响;② 休闲参与对心流体验和幸福感具有显著正向关系,且心流体验对幸福感具有显著正向影响;③ 心流体验对休闲效益具有正向显著影响,且休闲效益对幸福感具有正向显著影响;④ 心流体验在休闲参与对幸福感、休闲效益在心流体验对幸福感均具有显著中介效应;⑤ 旅游者不同背景变项在压力调适、休闲参与及幸福感等分别呈现不同程度的显著差异。     

Seasonal response of surface wind to SST perturbation in the Northern Hemisphere

The seasonal response of surface wind speed to sea surface temperature(SST)change in the Northern Hemisphere was investigated using 10 years(2002-2011)high-resolution satellite observations and reanalysis data.The results showed that correlation between surface wind speed perturbations and SST perturbations exhibits remarkable seasonal variation,with more positive correlation is stronger in the cold seasons than in the warm seasons.This seasonality in a positive correlation between SST and surface wind speed is attributable primarily to seasonal changes of oceanic and atmospheric background conditions in frontal regions.The mean SST gradient and the prevailing surface winds are strong in winter and weak in summer.Additionally,the eddy-induced response of surface wind speed is stronger in winter than in summer,although the locations and numbers of mesoscale eddies do not show obvious seasonal features.The response of surface wind speed is apparently due to stability and mixing within the marine atmospheric boundary layer(MABL),modulated by SST perturbations.In the cold seasons,the stronger positive(negative)SST perturbations are easier to increase(decrease)the MABL height and trigger(suppress)momentum vertical mixing,contributing to the positive correlation between SST and surface wind speed.In comparison,SST perturbations are relatively weak in the warm seasons,resulting in a weak response of surface wind speed to SST changes.This result holds for each individual region with energetic eddy activity in the Northern Hemisphere.     

Spatial patch structure and adaptive strategy for desert shrub of Reaumuria soongorica in arid ecosystem of the Heihe River Basin

In many arid ecosystems, vegetation frequently occurs in high-cover patches interspersed in a matrix of low plant cover. However, theoretical explanations for shrub patch pattern dynamics along climate gradients remain unclear on a large scale. This context aimed to assess the variance of the Reaumuria soongorica patch structure along the precipitation gradient and the factors that affect patch structure formation in the middle and lower Heihe River Basin (HRB). Field investigations on vegetation patterns and heterogeneity in soil properties were conducted during 2014 and 2015. The results showed that patch height, size and plant-to-patch distance were smaller in high precipitation habitats than in low precipitation sites. Climate, soil and vegetation explained 82.5% of the variance in patch structure. Spatially, R. soongorica shifted from a clumped to a random pattern on the landscape towards the MAP gradient, and heterogeneity in the surface soil properties (the ratio of biological soil crust (BSC) to bare gravels (BG)) determined the R. soongorica population distribution pattern in the middle and lower HRB. A conceptual model, which integrated water availability and plant facilitation and competition effects, was revealed that R. soongorica changed from a flexible water use strategy in high precipitation regions to a consistent water use strategy in low precipitation areas. Our study provides a comprehensive quantification of the variance in shrub patch structure along a precipitation gradient and may improve our understanding of vegetation pattern dynamics in the Gobi Desert under future climate change.

     

Understanding geographical patterns of a city’s diurnal rhythm from aggregate data of location‐aware services

Do collective behaviors of the daily routine of a city's inhabitants form the periodical cycling of human activity at the city level (here termed the “city's diurnal rhythm”)? If the answer is yes, do there exist geographical patterns in the city's diurnal rhythm? Using a nationwide dataset of observed uses of location‐aware services in the largest Chinese social media platform, we first confirm the significant periodicity in city‐level human activity from the perspective of the aggregate degree of social media uses over a day. We then investigate geographical changes in the diurnal rhythm of human activity and its local variations in different parts of the city, and between weekdays and weekend days, over 340 Chinese cities. Our results show that a city's diurnal rhythm across the whole country exhibits both regular, nationally conspicuous shifts along geographical gradients and locally distinct spatiotemporal changes within the city. Our findings could provide insights into the characterization of the daily routine of city‐level human activity and its geographical patterns, and have potential for several issues in terms of planning, management, and decision‐making related to human population dynamics.     

快速射电暴的数据统计

快速射电暴于2007年首次在脉冲星巡天的历史数据中被发现,是一种在射电波段观测到的具有色散量的单个强脉冲,其特征为持续时间仅为若干毫秒,峰值流量密度可达到央斯基量级。类似于射电脉冲星的单个脉冲,但其色散显著超过同一视线方向上银河系内星际介质的预期最大值。对截止2018年6月帕克斯望远镜、绿岸望远镜、阿雷西博射电望远镜、UTMOST望远镜和ASKAP望远镜已探测到的52例快速射电暴进行了观测量统计分析,根据模型扣除银河系星际介质导致的色散量后,快速射电暴平均色散量为584.5 pc·cm^-3,暗示着快速射电暴来自河外。通过最佳拟合估算红移幂律分布谱dN/dF_obs=4.14±1.30×F■sky^-1·day^-1。根据分析结果预计,FAST望远镜使用19波束接收机后,其多科学目标同时巡天在经过一年时间内能发现大约10个快速射电暴,将有效地扩大样本数量,为快速射电暴研究提供重要信息。     

The relationship between the radio core-dominance parameter and spectral index in different classes of extragalactic radio sources(Ⅱ)

Active galactic nuclei(AGNs) can be divided into two major classes, namely radio-loud and radio-quiet AGNs. A small subset of the radio-loud AGNs is called blazars, which are believed to be unified with Fanaroff-Riley type Ⅰ/Ⅱ(FRI/Ⅱ) radio galaxies. Following our previous work(Fan et al.), we present a sample of 2400 sources with measured radio flux densities of the core and extended components. The sample contains 250 BL Lacs, 520 quasars, 175 Seyferts, 1178 galaxies, 153 FRI or FRⅡ galaxies and 104 unidentified sources. We then calculate the radio core-dominance parameters and spectral indices, and study their relationship. Our analysis shows that the core-dominance parameters and spectral indices are quite different for different types of sources. We also confirm that the correlation between core-dominance parameter and spectral index exists for a large sample presented in this work.     

论海相页岩气富集机理——以四川盆地五峰组—龙马溪组为例

深入探究页岩气富集机理是保障勘探开发高效推进的基础。本研究通过对四川盆地五峰组—龙马溪组页岩气勘探开发实践的系统分析,梳理总结前人研究成果,从生成机理、运移机理、赋存机理和保存机理四个方面对海相页岩气富集机理进行了深入分析,并讨论了深层和常压页岩气的勘探开发潜力。结果表明：在生成机理方面,埋藏史和热演化史控制了页岩生排烃史、生排烃量和现今含气量;页岩气运移机理涉及运移动力、运移相态、运移方式和运移通道四方面内容,页岩气运移主要是烃源岩内的初次运移,同时讨论了初次运移的影响因素;在赋存机理方面,甲烷—页岩间表现出单/多分子层吸附和微孔充填等多种赋存机制,组分润湿性和孔隙有效性是决定甲烷吸附赋存和解吸运移的关键;在保存机理方面,盖层和物性自封闭是主要的保存机理,构造运动引起的裂缝—流体活动是页岩气保存条件遭到破坏的主要原因,流体活动时间和期次研究是页岩气保存条件和含气量定量评价的重要内容。页岩气富集机理的系统分析和创新认识为页岩气勘探开发评价提供了重要依据,建议加强页岩气演化历史全过程的动态评价。结合深层和常压页岩气勘探实践,分析了深层和常压页岩气的成因机制及主要特征,指出了下一步攻关内容及勘探方向。     

深部地球中水的分布和循环

深部地球中的主要物相(橄榄石、辉石、石榴石等及其高压相变产物)是理想化学式中不含H的“名义上无水矿物”,以缺陷形式存在于它们结构中的OH/H2O(统称为结构水)的发现是近二十年来地球科学领域最重要的进展之一。从天然样品的观察和高温高压实验结果来看,深部地球矿物中普遍含有结构水,其总量可能远远超过了水圈。水在深部地球不同层圈中的分布可能具有时间上和空间上的不均一性。在板块俯冲过程中,即使温压条件超过了含水矿物的稳定范围,名义上的无水矿物(如石榴石、辉石等)也可以携带大量的水(质量分数至少数百10-6)进入深部地球,构成了壳幔之间水循环的重要途径。