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71.
采用廉价的乳糖替代IPTG诱导重组别藻蓝蛋白(rAPC)在大肠杆菌(Escherichiacoli)JM109中表达,对诱导产物表达的培养基、培养条件、诱导剂的浓度和诱导时机进行了优化,将优化条件用于5L自控发酵罐进行高密度培养。结果表明,对rAPC表达的条件进行优化后,乳糖诱导rAPC的表达率可达26.8%,与IPTG的诱导结果接近;在高密度培养中,OD600最大达21.8。研究结果可为乳糖替代IPTG大规模生产rAPC奠定基础。 相似文献
72.
Spatial and Temporal Variations of Sound Speed at the PN Section 总被引:3,自引:0,他引:3
Gridded sound speed data were calculated using Del Grosso's formulation from the temperature and salinity data at the PN section
in the East China Sea covering 92 cruises between February 1978 and October 2000. The vertical gradients of sound speed are
mainly related to the seasonal variations, and the strong horizontal gradients are mainly related to the Kuroshio and the
upwelling. The standard deviations show that great variations of sound speed exist in the upper layer and in the slope zone.
Empirical orthogonal function analysis shows that contributions of surface heating and the Kuroshio to sound speed variance
are almost equivalent.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
73.
Hydrodynamic and sediment transport measurements from instrumentation deployed during a 54-day winter period at two sites on the Louisiana inner shelf are presented. Strong extratropical storms, with wind speeds of 7.8 to 15.1 m s-1, were the dominant forcing mechanism during the study. These typically caused mean oscillatory flows and shear velocities about 33% higher than fair weather (averaging 12.3 and 3.2 cm s-1 at the landward site, and 11.4 and 2.7 cm s-1 at the seaward site, respectively). These responses were coupled with mean near-bottom currents more than twice as strong as during fair weather (10.3 and 7.5 cm s-1 at the landward and seaward sites, respectively). These flowed in approximately the same direction as the veering wind, causing a net offshore transport of fine sand. Weak storms were responsible for little sediment transport whereas during fair weather, onshore sand transport of approximately 25-75% of the storm values appears to have occurred. This contradicts previous predictions of negligible fair-weather sediment movement on this inner shelf. 相似文献
74.
伶仃洋L_2和L_(16)孔第四纪有孔虫群与孢粉化石带特征及其地质意义 总被引:5,自引:0,他引:5
本文所依据的分析样品是取自珠江最大的一个河口湾伶仃洋中部的水下钻孔岩芯,其底部到达花岗岩基底。通过对第四纪有孔虫群与孢粉化石带特征的分析,阐明在相同的钻孔岩芯中,有孔虫分布变化所反映的古沉积环境特征与孢粉分析的古气候特征较为吻合,并与相应的沉积相对应,结合 ̄(14)C和 ̄(230)Th/ ̄(232)Th比值法测年数据,从而较好地重塑本区晚更新世中期以来的地质历史。 相似文献
75.
Remote estimation of surficial seafloor properties through the application Angular Range Analysis to multibeam sonar data 总被引:2,自引:2,他引:2
The variation of the backscatter strength with the angle of incidence is an intrinsic property of the seafloor, which can
be used in methods for acoustic seafloor characterization. Although multibeam sonars acquire backscatter over a wide range
of incidence angles, the angular information is normally neglected during standard backscatter processing and mosaicking.
An approach called Angular Range Analysis has been developed to preserve the backscatter angular information, and use it for
remote estimation of seafloor properties. Angular Range Analysis starts with the beam-by-beam time-series of acoustic backscatter
provided by the multibeam sonar and then corrects the backscatter for seafloor slope, beam pattern, time varying and angle
varying gains, and area of insonification. Subsequently a series of parameters are calculated from the stacking of consecutive
time series over a spatial scale that approximates half of the swath width. Based on these calculated parameters and the inversion
of an acoustic backscatter model, we estimate the acoustic impedance and the roughness of the insonified area on the seafloor.
In the process of this inversion, the behavior of the model parameters is constrained by established inter-property relationships.
The approach has been tested using a 300 kHz Simrad EM3000 multibeam sonar in Little Bay, NH. Impedance estimates are compared
to in situ measurements of sound speed. The comparison shows a very good correlation, indicating the potential of this approach for
robust seafloor characterization. 相似文献
76.
We collected surface water along the 142nd E meridian from Tasmania to Antarctica in December 1999. We measured temperature,
salinity and total chlorophyll a; additionally, we collected suspended particle size fractions and used fluorometric analysis to determine the quantity of
chlorophyll a in each of four cell size classes: picoplankton (<3 μm), two nanoplankton fractions (3–10 μm and 10–20 μm) and microplankton
(> 20 μm). Changes in temperature and salinity show that we crossed 6 water masses separated by 5 fronts. We found low abundance
(<0.2 mg m−3) of chlorophyll in all size classes, with the exception of higher values near the continent (0.2 to 0.4 mg m−3). Lowest chlorophyll values (<0.1 mg m−3) were found in the Polar Frontal Zone (51° to 54°S). Microplankton made up the largest portion of total chlorophyll throughout
most of the region. We conclude that biomass of all phytoplankton fractions, especially pico-and nanoplankton, was constrained
by limiting factors, most probably iron, throughout the region and that ecosystem dynamics within a zone are not circumpolar
but are regionalized within sectors. 相似文献
77.
Young-Ok Kim Eun Jin Yang Jung-Hoon Kang Kyoungsoon Shin Man Chang Cheol Soo Myung 《Ocean Science Journal》2007,42(1):9-17
The summer distributions of planktonic microbial communities (heterotrophic and phtosynthetic bacteria, phtosynthetic and
heterotrophic nanoflagellates, ciliate plankton, and microphytoplankton) were compared between inner and outer areas of Lake
Sihwa, divided by an artificial breakwater, located on the western coast of Korea, in September 2003. The semienclosed, inner
area was characterized by hyposaline surface water (<17 psu), and by low concentrations of dissolved oxygen (avg. 0.4 mg L1) and high concentrations of inorganic nutrients (nitrogenous nutrients >36 μM, phosphate <4 μM) in the bottom layer. Higher
densities of heterotrophic bacteria and nanoflagellates also occurred in the inner area than did in the outer area, while
microphytoplankton (mainly diatoms) occurred abundantly in the outer area. A tiny tintinnid ciliate, Tintinnopsis nana, bloomed
into more than 106 cells L1 at the surface layer of the inner area, while its abundance was much lower (103-104 cells L1) in the outer area of the breakwater. Ciliate abundance was highly correlated with heterotrophic bacteria (r = 0.886, p <
0.001) and heterotrophic flagellates (r = 0.962, p < 0.001), indicating that rich food availability may have led to theT. nana bloom. These results suggest that the breakwater causes the eutrophic environment in artificial lakes with limited flushing
of enriched water and develops into abundant bacteria, nanoflagellates, and ciliates. 相似文献
78.
79.
The recent sea-ice reduction in the Arctic Ocean is not spatially uniform, but is disproportionally large around the Northwind
Ridge and Chukchi Plateau compared to elsewhere in the Canada Basin. In the Northwind Ridge region, Pacific Summer Water (PSW)
delivered from the Bering Sea occupies the subsurface layer. The spatial distribution of warm PSW shows a quite similar pattern
to the recent ice retreat, suggesting the influence of PSW on the sea-ice reduction. To understand the regionality of the
recent ice retreat, we examine the dynamics and timing of the delivery of the PSW into this region. Here, we adopt a two-layer
linearized potential vorticity equation to investigate the behavior of Rossby waves in the presence of a topographic discontinuity
in the high latitude ocean. The analytical results show a quite different structure from those of mid-latitude basins due
to the small value of β. Incident barotropic waves excited by the sea-ice motion with large annual variation can be scattered
into both barotropic and baroclinic modes at the discontinuity. Since the scattered baroclinic Rossby wave with annual frequency
cannot propagate freely, a strong baroclinic current near the topographic discontinuity is established. The seasonal variation
of current near the topographic discontinuity would cause a kind of selective switching system for shelf water transport into
the basin. In our simple analytical model, the enhanced northward transport of summer water and reduced northward transport
of winter water are well demonstrated. The present study indicates that these basic dynamics imply that a strengthening of
the surface forcing during winter in the Canada Basin could cause sea-ice reduction in the Western Arctic through the changes
of underlying Pacific Summer Water. 相似文献
80.
作者在已完成大气激光雷达系统夜间工作的基础上 ,对白天工作条件下的各种环境背景光的干扰进行了理论与实验分析 ,得出太阳直射光和天空光为白天工作的主要背景干扰。实验结果表明 ,经过激光发射与接收视场角严格匹配及使用干涉滤光器 (2 .6 nm和 0 .15nm)进行窄带滤波 ,背景干扰被明显地剔除 ,可进一步压低背景干扰约 2 0倍。证明采取视场匹配及干涉滤光器的措施可基本保证激光雷达系统在白天条件下工作。 相似文献