A comparison of cytochrome P4501A (CYP1A) mRNA inducibility in four species of Atlantic coast anadromous fishes

Quantification of levels of cytochrome P4501A (CYP1A) gene expression in sentinel species of fishes has been proposed as a management tool to evaluate contamination of aquatic systems. Based on preliminary studies, we hypothesized that differences in CYP1A mRNA inducibility among individuals, populations, or species might lead to spurious conclusions when using this approach in environmental monitoring programs. To address this possibility, we quantitated and compared CYP1A mRNA induction levels in four species of common Atlantic Coast estuarine fish: smooth flounder, hogchoker, striped bass, and Atlantic tomcod, which were treated with model chemicals (beta naphthoflavone (β-NF), or benzo[a]pyrene at 10 ppm) known to induce CYP1A mRNA, or were exposed to contaminated environments. Species-specific CYP1A DNA probes were generated from PCR (polymerase chain reaction) amplification of genomic DNA using conserved oligonucleotide primers, and, along with cloned rainbow trout and Atlantic tomcod CYP1A cDNA probes were used to quantify CYP1A mRNA levels in northern blot analyses. Successful PCR amplification of CYP1A hybridizable DNA fragments was observed for all four species. Results from northern blot analyses showed large differences in CYP1A mRNA induction among species; only Atlantic tomcod exhibited significant induction of CYP1A mRNA for both chemically treated (97-fold) and environmentally exposed fish (34-fold). Significant, although lower, levels of induction were observed in β-NF treated (14-fold) smooth flounder, but not in environmentally exposed smooth flounder. Only low levels (not significant) of CYP1A gene induction were detected in hogchokers and striped bass. We conclude that CYP1A mRNA inducibility differed significantly among fish taxa perhaps due to differences in regulation of gene expression, suggesting that careful selection of sentinel species should be exercised prior to the use of CYP1A mRNA induction in environmental monitoring programs. However, the significance of differences in CYP1A mRNA inducibility in relation to higher level biological endpoints has yet to be determined.     

The transformation and export of phosphorus from Wetlands

There is widespread acceptance of the phosphorus retention capability of wetlands even though research findings are often inconclusive and contradictory. The results of a one year phosphorus budget study indicate that internal wetland processes may transform sediment bound phosphorus to plant available orthophosphorus. While total phosphorus imports were nearly double the total phosphorus exports for the study wetland, orthophosphorus exports were 22 per cent greater than imports. This study supports the recent finding that wetlands have limited capability to retain orthophosphorus and indicates that wetlands may even increase the export of orthophosphorus. The generally accepted nutrient retention function of wetlands and their possible role in eutrophication is thus questionable.     

Comparison of heat and bromide as ground water tracers near streams

Heat and bromide were compared as tracers for examining stream/ground water exchanges along the middle reaches of the Santa Clara River, California, during a 10-hour surface water sodium bromide injection test. Three cross sections that comprise six shallow (<1 m) piezometers were installed at the upper, middle, and lower sections of a 17 km long study reach, to monitor temperatures and bromide concentrations in the shallow ground water beneath the stream. A heat and ground water transport simulation model and a closely related solute and ground water transport simulation model were matched up for comparison of simulated and observed temperatures and bromide concentrations in the streambed. Vertical, one-dimensional simulations of sediment temperature were fitted to observed temperature results, to yield apparent streambed hydraulic conductivities in each cross section. The temperature-based hydraulic conductivities were assigned to a solute and ground water transport model to predict sediment bromide concentrations, during the sodium bromide injection test. Vertical, one-dimensional simulations of bromide concentrations in the sediments yielded a good match to the observed bromide concentrations, without adjustment of any model parameters except solute dispersivities. This indicates that, for the spatial and temporal scales examined on the Santa Clara River, the use of heat and bromide as tracers provide comparable information with respect to apparent hydraulic conductivities and fluxes for sediments near streams. In other settings, caution should be used due to differences in the nature of conservative (bromide) versus nonconservative (heat) tracers, particularly when preferential flowpaths are present.     

Look out, aurorae about!

Jim Wild recounts the rationale and success of AuroraWatch, a service that alerts you when you might be able to see aurorae.     

Discussion of: Ortega-Flores et al. (2018) Provenance analysis of Oligocene sandstone from the Cerro Pelón area,southern Gulf of Mexico.https://doi.org/10.1080/00206814.2018.1476922

ABSTRACT

We discuss the 2018 publication that reports petrographic, heavy mineral data, mineral chemistry, and zircon geochronology for Oligocene sandstones in the Cerro Pelón area in southern Mexico Sureste basin. As the title of their paper says, the goal of their study is to establish the source (s) of the voluminous Cenozoic section in this region, reaching several kilometres in thickness and important as a petroleum system. These authors conclude that Oligocene sandstones of La Laja Formation were mostly sourced from eclogite- to greenschist-facies metasedimentary, metaigneous, and ultramafic rocks of the Guatemala suture complex. Minor contributions from the Chiapas Massif Complex, exposed directly to the south ~60 km of the Cerro Pelón area, were also suggested by the authors. They thus conclude that the Palaeogene stratigraphic record in southeastern Mexico was mostly controlled by the development of the Caribbean–North America plate boundary rather than by orogenic processes at the Pacific margin of North America. Presently, we do not agree with the conclusions of Ortega Flores and colleagues who studied the Cerro Pelón section, thus some discussion is required. Serpentinite bearing Nanchital Conglomerate is well exposed in the Cerro Pelón area, and high- to low-grade metamorphic rocks experienced an uplift in the vicinity of the Cerro Pelón area at the time of deposition of the La Laja Formation. We believe the data are better explained by multiple local sources in southern and eastern Oaxaca as well as sources to the south and southwest, which include the Cenozoic coastal batholith, the Grenvillean/Guichicovi basement complexes, the Chiapas Massif, the Mazatlán schist and other units in the Cuicateco Belt, as well as the Mesozoic cover of these areas (Todos Santos Formation, Cretaceous carbonate rocks, and Paleogene strata such as the Soyaló and Bosque Formations).     

On the Intensity Thresholds of the Network and Plage Regions

A period of minimum solar activity in April and May 1996 was used to analyze full-disk CaK spectroheliograms with the aim of deriving the intensity thresholds of the quiet network and the plages by applying the so-called inflexion point method. The average network intensity threshold is found to be influenced mainly by the seeing, whereas the average plage threshold shows an increasing trend from disk center towards the solar limb. Both parameters are compared with the results of other authors.     

Competition among destinations in spatial interaction models: a new point of view

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A geostatistically based inverse model for three-dimensional variably saturated flow

We present a geostatistically based inverse model for characterizing heterogeneity in parameters of unsaturated hydraulic conductivity for three-dimensional flow. Pressure and moisture content are related to perturbations in hydraulic parameters through cross-covariances, which are calculated to first-order. Sensitivities needed for covariance calculations are derived using the adjoint state sensitivity method. Approximations of the conditional mean parameter fields are then obtained from the cokriging estimator. Correlation between parameters and pressure – moisture content perturbations is seen to be strongly dependent on mean pressure or moisture content. High correlation between parameters and pressure data was obtained under saturated or near saturated flow conditions, providing accurate estimation of saturated hydraulic conductivity, while moisture content measurements provided accurate estimation of the pore size distribution parameter under unsaturated flow conditions.