近40年来海州湾海岸线时空变化分析

以Landsat MSS、TM、ETM+影像和HJ-1A CCD1影像为数据源,对海州湾大陆海岸线1973-2013年的变迁进行了连续监测,定量获取了海岸线信息,解译了岸线摆动区内土地利用类型,对近40 a来海州湾大陆海岸线时空变化和海岸开发方式进行了系统分析.结果表明:海州湾岸线长度及类型动态变化显著,岸线长度整体增加,海湾面积不断减少,岸线类型以人工岸线为主;岸线整体向海推进导致陆域面积净增65.54 km2,变迁速率时空分布不均,变迁主要发生在岚山港、绣针河口至柘汪河口、兴庄河口至西墅、连云港港岸段,城镇扩张导致20t0-2013年变迁最为剧烈,速率达122.9m/a;海岸人为开发是海州湾岸线变化的主导因素,且开发方式时间异质性显著,早期以盐业、养殖业为主,20世纪80年代开始港口码头建设比例显著增加,进入新世纪以来,用于城镇建设的围填海规模大幅增长,尤其是2010年之后已成为海州湾地区海岸开发的首要方式.     

一株海洋微藻的分离鉴定及其产油条件优化

自青岛近岸海水中分离到一株硅藻,经形态学和分子生物学鉴定,表明该藻与小头菱形藻(Nitzschia microcephala)最为相近,18SrDNA相似性99%以上。研究以f/2培养基为基础,对该藻的生长和油脂积累条件进行探析,表明其在pH=6~8时具有较高的生长速率,最大可达到0.01d-1,油脂含量随pH的升高而降低;添加SiO2-3可以抑制菱形藻的生长和油脂积累;高浓度的Fe3+能促进菱形藻的生长;在Zn2+浓度为1.74μmol/L的条件下,菱形藻能够较好地生长并且进行油脂的积累;其中pH、SiO2-3和Fe3+是影响该藻生长和油脂积累的主要因子。正交试验研究表明,该藻在f/2培养液中生长和油脂积累的最优条件为初始pH=9,SiO2-3和Fe3+添加浓度分别为0.07和100μmol/L。在该最优条件下,该藻的生物量和油脂积累可分别达到94.7mg/L和19.6%。     

太平洋鳕鱼脑中硫苷脂的分离纯化和分析

以太平洋鳕鱼为原料,从鳕鱼脑中分离硫苷脂,确立了提取及纯化的条件,并对硫苷脂的纯度和分子种进行分析。首先,采用氯仿/甲醇(2∶1,v/v)提取总脂。然后,依次采用氯仿/甲醇/水(7∶3∶0.3,v/v/v)洗脱硅胶柱层析,含0.2mol/L乙酸铵的氯仿/甲醇/水(30∶60∶8,v/v/v)洗脱DEAE Sephadex-A25离子交换柱层析,40%甲醇脱盐和100%甲醇洗脱反相C8柱层析,获得硫苷脂纯品。最后,利用500YMC Diol液相色谱柱(3.0mm×250mm,5μm)分离,以正己烷/异丙醇(70∶30,v/v),异丙醇/水/甲酸/氨水(100∶13∶1∶0.14,v/v/v/v)为流动相梯度洗脱,采用负电喷雾电离(ESI)和母离子扫描模式,用液相色谱-串联质谱(LC-MS/MS)法分析了鳕鱼脑硫苷脂的纯度和分子种组成,并比较了其与哺乳动物脑中硫苷脂分子种组成的异同。结果表明,本实验制得的鳕鱼脑硫苷脂纯度为90.74%,与哺乳动物类似,鳕鱼脑硫苷脂的长链碱基以鞘氨醇为主,主要分子种为d18∶1-C24∶1,但脂肪酸的羟基化程度略低,且含少量独特的分子种,如d18∶1-22∶1和d18∶2-25∶2。     

大数据时代的人类移动性研究

人类个体/群体移动特征是多学科共同关注的研究主题。移动定位、无线通讯和移动互联网技术的快速发展使得获取大规模、长时间序列、精细时空粒度的个体移动轨迹和相互作用定量化成为可能。同时,地理信息科学、统计物理学、复杂网络科学和计算机科学等多学科交叉也为人类移动性研究的定量化提供了有力支撑。本文首先系统总结了大数据时代开展人类移动性研究的多源异构数据基础和多学科研究方法,然后将人类移动性研究归纳为面向人和面向地理空间两大方向。面向人的研究侧重探索人类移动特性的统计规律,并建立模型解释相应的动力学机制,或分析人类活动模式,并预测出行或活动;面向地理空间的研究侧重从地理视角分析人类群体在地理空间中的移动,探索宏观活动和地理空间的交互特征。围绕这两大方向,本文评述了人类移动性的研究进展和存在问题,认为人类移动性研究在数据稀疏性、数据偏斜影响与处理、多源异构数据挖掘、机器学习方法等方面依然面临挑战,对多学科研究方法的交叉与融合提出了更高要求。     

ˮ���ǡ���ֱ�ڼ�ˮƽ�ڸ�Ƶ�μ�¼���ݲ���ĶԱ�

????峱???????????????????沨??????????????????????????б???????????????????????????????????????????????????????????????????????????????????????峱??????????????????????￡???????????????沨?????????????????????????????????????????????????????????????????μ??????????????????????μ????     

Modeling seasonal variations of subsurface chlorophyll maximum in South China Sea

In the South China Sea(SCS), the subsurface chlorophyll maximum(SCM) is frequently observed while the mechanisms of SCM occurrence have not been well understood. In this study, a 1-D physical-biochemical coupled model was used to study the seasonal variations of vertical profiles of chlorophyll-a(Chl-a) in the SCS. Three parameters(i.e., SCM layer(SCML) depth, thickness, and intensity) were defined to characterize the vertical distribution of Chl-a in SCML and were obtained by fitting the vertical profile of Chl-a in the subsurface layer using a Gaussian function. The seasonal variations of SCMs are reproduced reasonably well compared to the observations. The annual averages of SCML depth, thickness, and intensity are 75 ± 10 m, 31 ± 6.7 m, and 0.37 ± 0.11 mg m-3, respectively. A thick, close to surface SCML together with a higher intensity occurs during the northeastern monsoon. Both the SCML thickness and intensity are sensitive to the changes of surface wind speed in winter and summer, but the surface wind speed exerts a minor influence on the SCML depth; for example, double strengthening of the southwestern monsoon in summer can lead to the thickening of SCML by 46%, the intensity decreasing by 30%, and the shoaling by 6%. This is because part of nutrients are pumped from the upper nutricline to the surface mixed layer by strong vertical mixing. Increasing initial nutrient concentrations by two times will increase the intensity of SCML by over 80% in winter and spring. The sensitivity analysis indicates that light attenuation is critical to the three parameters of SCM. Decreasing background light attenuation by 20% extends the euphotic zone, makes SCML deeper(~20%) and thicker(12% – 41%), and increases the intensity by over 16%. Overall, the depth of SCML is mainly controlled by light attenuation, and the SCML thickness and intensity are closely associated with wind and initial nitrate concentration in the SCS.     

Analysis of genetic diversity and differentiation of seven stocks of Litopenaeus vannamei using microsatellite markers

Seven microsatellite markers were used to evaluate the genetic diversity and differentiation of seven stocks of Litopenaeus vannamei, which were introduced from Central and South America to China. All seven microsatellite loci were polymorphic, with polymorphism information content(PIC) values ranging from 0.593 to 0.952. Totally 92 alleles were identified, and the number of alleles(Na) and effective alleles(Ne) varied between 4 and 21 and 2.7 and 14.6, respectively. Observed heterozygosity(Ho) values were lower than the expected heterozygosity(He) values(0.526–0.754), which indicated that the seven stocks possessed a rich genetic diversity. Thirty-seven tests were detected for reasonable significant deviation from Hardy-Weinberg equilibrium. Fis values were positive at five loci, suggesting that there was a relatively high degree of inbreeding within stocks. Pairwise Fst values ranged from 0.0225 to 0.151, and most of the stock pairs were moderately differentiated. Genetic distance and cluster analysis using UPGMA revealed a close genetic relationship of L. vannamei between Pop2 and Pop3. AMOVA indicated that the genetic variation among stocks(11.3%) was much lower than that within stocks(88.7%). Although the seven stocks had a certain degree of genetic differentiation and a rich genetic diversity, there is an increasing risk of decreased performance due to inbreeding in subsequent generations.     

四川盆地东部垫江盐盆早三叠世嘉陵江组四段杂卤石成因及对成钾的指示

四川盆地三叠纪是主要的成钾期,目前发现的主要含钾矿物为杂卤石,有关早三叠世嘉陵江组四段石盐岩中杂卤石成因一直存在争议.采用薄片鉴定、扫描电镜、稀土元素和锶同位素等手段,分析了四川盆地东部垫江盐盆长寿地区嘉陵江组四段石盐岩中杂卤石矿物形态特征,初步探讨了该杂卤石成因及对寻找海相钾盐的指示意义.扫描电镜下与石盐岩共生的杂卤...     

延安地区大气中PM_(10)浓度水平及其变化特征

利用延安地区2006—2008年大气中可吸入颗粒物PM10的监测资料,分析了PM10的浓度水平、变化规律及其影响因素。延安地区PM10的年平均浓度为153μg/m3,超过国家环境空气质量标准年平均值2级浓度限值70μg/m3的1倍多,这与当地高的PM10区域背景水平(平均值为109μg/m3)紧密相关。PM10质量浓度水平具有明显的季节性,春季最高,冬秋其次,夏季最低。PM10日均值浓度超过国家环境空气质量2级标准的日数占全年的40%,其中春季达66%。PM10质量浓度日变化呈明显的双峰双谷型特征,与风速的日变化有明显的反相关关系。1mm降水量对PM10的湿清除能力按冬、春、秋、夏依次递减。延安地区出现西风或偏西风时PM10平均质量浓度高,但就累积影响而言,处于主导风向上的西南风要比其他方向高40~50μg/m3左右。     

凡纳滨对虾(Litopenaeus vannamei)Runt转录因子的基因克隆及其功能分析

Runt结构域转录因子家族(Runx)在哺乳动物体内存在三种基因型,分别起着造血,骨生成和控制上皮细胞增殖的作用。本文应用反转录和c DNA末端快速扩增(RACE)技术,首次在凡纳滨对虾(Litopenaeus vannamei)中克隆并测序了一种Runt(lvrunt)基因全长。lvrunt c DNA全长1754bp,含有1个663bp长的开放阅读框,编码221个氨基酸,理论分子量为23.6k Da,具有Runt-family结构域。对凡纳滨对虾鳃、肠、肝胰腺、类淋巴、心脏、肌肉组织和血淋巴lvrunt的转录特征进行分析,结果显示该基因几乎特异性地在血淋巴细胞中表达,而在其他组织中表达较低。肌肉注射重组造血激素蛋白(r AST)或白斑综合征病毒(WSSV)粗提液均可显著提高lvrunt在凡纳滨对虾体内的转录表达。上述结果提示,lvrunt主要在血细胞发挥作用,可能作为造血激素的下游基因起到调节血细胞增殖和分化的作用,并在受到病毒感染后,参与提升血细胞数量或者促进血细胞分化的过程。