Studies on the eggs and larvae,ofSardinella aurita (Cuv. & Val.) in the fishing grounds of Southern Fujian and the Taiwan Bank

In this paper, the morphological characters of eggs and larvae ofSardinella aurita (Cuvier & Valenciennes), its spawning ground, spawning seasons and spawning conditions have been studied. Sardinella aurita likes to breed in the upwelling area and selects the Waixie fishing ground as its main spawning ground. The months from February to September are its spawning seasons, reaching its peak in April. In the main spawning ground, the temperature of the surface layer was found to be 24.4–25.2°C, the salinity 33.87–34.07%. and the depth of water between 34–60m. The distribution of the larvae is closely related to the distribution of plankton, the path of migration of adult fish, and the current direction of the water system. In order to protect fishery resources, it is necessary to prohibit catching the spawning fish in the Waixie fishing ground in April, and the catching of immatures from March to June. This paper was published in Chinese inOcean. Limn. Sinica 14(3): 240–248, 1983.     

Scattering of a plane wave by a lined cylindrical cavity in a poroelastic half-plane

The focus of this contribution is to develop a complex variable function method to solve the two-dimensional scattering of plane waves by a lined cylindrical cavity in the poroelastic half-plane. The poroelastic half-plane is based on Biot’s dynamic theory, and the governing equations are solved by reduction to three Helmholtz equations. The lining structure can be treated as an elastic material and decoupled into two Helmholtz equations. Here, the large circle assumption is applied to simulate the half-plane boundary. By using appropriate boundary conditions and continuity conditions, the unknown coefficients in the potentials can be determined. Selected numerical results are presented in this paper.     

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Expression and enzymatic characterization of a cold-adapted β-agarase from Antarctic bacterium Pseudoalteromonas sp.NJ21

An agar-degrading bacterium,designated as Pseudoalteromonas sp. NJ21,was isolated from an Antarctic sediment sample. The agarase gene a ga1161 from Pseudoalteromonas sp. NJ21 consisting of a 2 382-bp coding region was cloned. The gene encodes a 793-amino acids protein and was found to possess characteristic features of the Glyco_hydro_42 family. The recombinant agarase(r Aga1161) was overexpressed in Escherichia coli and purified as a fusion protein. Enzyme activity analysis revealed that the optimum temperature and pH for the purified recombinant agarase were 30–40°C and 8.0,respectively. rAga1161 was found to maintain as much as 80% of its maximum activity at 10°C,which is typical of a coldadapted enzyme. The pattern of agar hydrolysis demonstrated that the enzyme is an β-agarase,producing neoagarobiose(NA2) as the final main product. Furthermore,this work is the first proof of an agarolytic activity in Antarctic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine,food and cosmetic industries.     

Biomarker responses in the bivalve Chlamys farreri to the water-soluble fraction of crude oil

To investigate the effect of the water soluble fraction of crude oil(WSF) on marine bivalves, the scallop C hlamys farreri was exposed to three WSF concentrations(0.18 mg/L, 0.32 mg/L, and 0.51 mg/L, respectively) in seawater. Petroleum hydrocarbon contents in scallops and a suite of enzymes [7-Ethoxyresorufin-O-deethylase(EROD), aryl hydrocarbon hydroxylase(AHH), glutathione S-transferase(GST), and glutathione peroxidase(GPx)] in gills and digestive glands were monitored over 10 days. The results revealed that WSF affected the activity of the four enzymes in the gills and digestive glands. EROD activity in the gills was significantly induced in most individuals of the three test groups, while in the digestive gland it was significantly induced in the low-concentration group within 4 days but was inhibited in the middle- and high-concentration groups on days 1, 4, and 10. AHH activity in the gills of all treatment groups was significantly induced on day 1. In the digestive gland, AHH activity was induced in most individuals from the treatment groups. In all treatment groups, GST activity was significantly inhibited from days 2 to 10 in the gills and was induced after day 4 in the digestive gland. GPx activity in the gills was significantly inhibited throughout the exposure period in all treatment groups. There was no overall significant difference in GPx activity in the digestive gland between the control and treatment groups. Our results also revealed that petroleum hydrocarbon concentrations in the tissues increased linearly with exposure time. EROD activity in the digestive gland and GST and GPx activity in the gill tissue were negatively correlated with petroleum hydrocarbon body burden. These enzymes play important roles in detoxification and can act as potential biomarkers for monitoring petroleum hydrocarbon contaminants in the marine environment.     

DEM analyses of crack propagation in flawed rock mass under uniaxial compressionEI北大核心CSCD

A contact model for rock is established and imbedded into a DEM software by summarizing the bond granule tests. DEM simulation of uniaxial compression test on the pre-cracked Lac du Bonnet granite is performed, and then stress distributions are further analyzed and compared with the theoretical results. Different fracture criteria are employed to predict the crack initiation angles that are compared with theoretical ones. The results show that the failure modes obtained from DEM simulation are similar to experimental results, and stress distributions in DEM simulation are qualitatively similar to theoretical values. When the angle of pre-crack is small, the lateral stresses are compressive and tensile. The compressive strains concentrate at two edges, resulting in the tensile strains in the up-and downward cracks. When the angle of the pre-crack is large enough, the stress concentration is unobvious, leading to a discrepancy between the DEM and theoretical results. The crack extension angle resulting from uniaxial compression measured from DEM tests are in good agreement with those acquired from experimental tests. These angles are consistent with theoretical predictions by the maximum circumferential stress criterion and the maximum energy release rate criterion.     

广西红树林根际土壤放线菌的原位培养分离及其活性筛选

为了发掘广西红树林根际土壤的放线菌资源,本文利用原位培养装置,埋于根际土壤中俘获放线菌,30d后取回实验室,采用平板涂布法对4个地点的原位培养样品于15种培养基上进行分离纯化;对分离株基于16SrRNA基因序列进行系统发育分析;进一步进行抗菌活性和产酶活性检测。共分离得到113株放线菌。对其中33株放线菌进行测序,结果表明20株属于链霉菌属,11株属于拟诺卡氏菌属,1株属于伦兹氏菌属,1株与拟诺卡氏菌属相似性最高为90%,很可能属于放线菌一个新属。抗菌实验结果显示其中有7株、4株、18株、6株、10株、3株实验菌株分别对大肠埃希菌、铜绿假单胞菌、金黄色葡萄球菌、普通变形杆菌、乙型溶血性链球菌和肺炎克雷伯氏菌具有抑制作用;有55株、62株、24株、72株的实验菌株分别具有纤维素酶活性、淀粉酶活性、胶原蛋白酶活性、酯酶活性。原位培养可以丰富对广西红树林根际土壤的认识,分离到了新种甚至可能是新属的放线菌,分离得到的部分放线菌菌株具有较高生物活性,为后续工作提供了良好的实验材料。     

大别造山带红安地块康家湾构造混杂岩锆石U- Pb年龄和Hf同位素组成及其地质意义

扬子克拉通北缘中元古代—新元古代地质构造演化一直是地学界研究的热点。本文报道了大别造山带红安地块康家湾混杂岩中榴闪岩岩块和含石榴子石白云钠长片岩基质中锆石U-Pb年龄和Hf同位素组成。结果表明,康家湾混杂岩中榴闪岩岩块的原岩形成时间为1064±10 Ma,且经历了183±3 Ma的流体作用过程;中元古代锆石Hf同位素组成(ε_Hf(t)=4.7～12.3)接近当时的亏损地幔值,指示榴闪岩原岩(玄武质岩石)可能来自亏损的地幔源区部分熔融作用;混杂基质中碎屑锆石年龄谱可大致分为2576 Ma、2065～1698 Ma、1071～560 Ma和254～156 Ma四组,暗示其物源可能主要来自于近源的秦岭-大别造山带前寒武纪基底;基质中最年轻的岩浆锆石年龄为～240 Ma,表明康家湾混杂岩的最终就位时间可能在晚三叠世到早侏罗世之间。综合秦岭-大别造山带新元古代构造演化过程,本研究认为大别造山带红安地块与扬子克拉通庙湾蛇绿岩、神农架弧等共同构成了一套中元古代末期—新元古代早期岛弧增生造山体系,从而支持扬子克拉通可能由一系列微陆块逐渐拼合而成。     

面向国家重大战略工程需求的测绘类人才培养模式探索

探索和构建创新型人才培养体系和模式,切实满足行业高素质人才需求、服务国家及地方经济建设是高校专业人才培养的终极目标.本文以兰州交通大学测绘学科为例,对测绘类人才培养模式进行探索与分析:1)系统剖析兰州交通大学测绘类专业实践课程内容设置、跨度6年的应届毕业生生源及就业流向趋势,总结了现有条件下人才培养的不足与局限;2)针对上述问题,尝试构建5个"3"模式教学体系、实践教学与示范中心建设、多层次学科技能竞赛体系与专业认证、国际视野引导四个专业建设的发展模式,力求培养学生专业方面的通识能力、就业渠道及行业方面扩展技能和解决实际问题的综合创新能力;3)立足扎根西部和面向铁路交通行业特色办学背景,力图培养吃苦耐劳、艰苦奋斗、多学科交叉的高素质专业人才,以期为"川藏铁路"等国家重大战略工程建设提供科技和人才保障.     

基于InSAR技术城市垃圾场存量垃圾填埋量的估计

城市垃圾治理是生态文明建设、城市精细化管理的必然要求.有效评估城市垃圾场存量垃圾填埋量,提升处理技术,减少垃圾污染是城市管理部门首要解决的问题.由于早期垃圾场采用深挖填埋式,且建立时间较长,很难获得垃圾填埋数量.通过调查雷达卫星存档数据,利用InSAR技术得到不同时期的数字高程模型数据,进而计算得到哈尔滨市某垃圾场历史存量垃圾数据.