黄渤海底层盐度预报方法的研究

根据二维流体动力学方程和深度平均盐度扩散方程在河口径流量以及蒸发和降水之差为已知情况下构成的闭合方程组,预报出深度平均盐度,然后利用底层盐度与深度平均盐度、水深和时间(月)之间的经验关系,给出底层盐度的二维预报。为了检验试报结果的可靠性,文中将黄渤海底层盐度的试报结果(1979年7月11日,时效为3d)与标准断面观测资料(7月4—14日观测,124.5°E以西,共104站)作一粗略比较。比较表明,试报结果与实测值的相关系数为0.96,均方误差为α=0.26,绝对误差小于0.2和0.3的站数分别占总站数的63.5％和77.0％,而总均绝差为0.19。由此可见,试报的效果是令人满意的。     

符合药用质量要求的甲壳素精制工艺

本文探讨了符合以下药用质量要求的甲壳素的精制工艺，甲壳素脱乙酰度在７０－９０％之间，钙的含量〈０．５％，总蛋含量在７－８％之间，灰分〈１％，酸不溶性灰分〈０．０５％，无毒，为甲壳素在医药上的应用，提供了生产数据。     

温度对罗非鱼下脚料蛋白酶解液混合营养培养螺旋藻的影响

在常规的Zarrouk培养基中添加1000∶3(v/v)的罗非鱼下脚料蛋白酶解液对钝顶螺旋藻进行混合营养培养,考察在光照强度230μmol/m2.s、温度15～40℃范围内对螺旋藻生物量、叶绿素、藻胆蛋白和蛋白质含量的影响。结果表明,添加酶解液在15～35℃时能够显著提高螺旋藻生物量,35℃时增幅最大为38.2%;在30～40℃时添加酶解液显著提高螺旋藻叶绿素的含量,30℃时含量提高了11.7%;在15～20℃低温条件下添加酶解液显著提高螺旋藻藻胆蛋白含量,15℃时增幅达15.1%;在15～35℃范围内添加酶解液显著提高蛋白质含量,30℃时增幅最大为13.7%。     

中国北部黄海近岸水体周丛硅藻的群落群集动态特征的研究

The colonization features of periphytic diatoms were studied in coastal waters of the Yellow Sea, northern China from May to June 2010, using glass slides as an artificial substratum. Samples were collected at a time interval of 1, 3, 7, 10, 14, 21 and 28 d from two depths of 1 and 3 m. The dynamics of diatom colonization process had a similar pattern in community structure and fitted the logistic model in growth curve at both depths. The maximum abundance and the time for reaching 50% maximum abundance （10 d） showed no significant differences （P〉0.05） between two depths 1 and 3 m. Although the diatom communities repre- sented similar taxonomic composition, they differed in the temporal pattern of structural parameters and in succession dynamics of dominant species between the two layers. The species richness showed significantly higher values during the colonization period more than 14 d, while the species diversity and evenness rep- resented a higher variability with significantly different values （P〈0.05） at a depth of 1 m than at a deeper layer. The results suggest that the diatom colonization follows the logistic model growth curve and differs in colonization features between different depths in the coastal waters, and that the sampling strategy at i m is more effective to detect the ecological features for bioassessment in marine ecosystems.     

基于合成孔径雷达回波信号的海洋溢油监测方法研究

海洋油污染是各类海洋污染中最常见、分布面积最广且危害程度最大的污染之一。近年来,海洋特别是近海人类活动频繁,且随着海上运输和石油加工业的发展,油田井喷、钻井平台爆炸、船舶碰撞等所造成的溢油事故增多,因而,监测海洋溢油具有重要的经济和社会现实意义。研究采用MatLAB工具,通过图像预处理(图像校正和增强)、特征提取和神经网络识别等方法,对合成孔径雷达(SAR)海洋溢油图像进行处理,最终期望实现半自动区分SAR图像上各类目标,并进行多种神经网络方法效果比较。研究首先对SAR海洋溢油图像进行初步人工识别;然后进行图像预处理(几何校正、滤波处理等)和基于灰度共生矩阵的特征值计算;最后,借助神经网络方法对溢油区域和疑似溢油区域进行分类,输出分类处理后的图像。通过输出图像分析发现,神经网络能对SAR海洋溢油图像中溢油、海水、土地3类目标进行明确分类,且RBF神经网络模型精度高于BP神经网络。本文提出的半自动分类方法不仅能提高SAR图像处理效率,将分类目标扩充有溢油和非溢油扩充到溢油、海水、土地3类,提高图像处理的全面性,同时通过比较RBF和BP神经网络在SAR溢油图像分类上的具体优劣,有着较好实际意义。     

渤海莱州湾地区晚更新世以来有孔虫特征反映的古环境变化

Controlled by climate changes, there were three large-scale transgressions and regressions around the Bohai Sea during the late Quaternary, which were accepted by most geologists. However, a big controversy still exists about the time when the transgressions occurred separately. In order to find out the process of the paleoenvironmental changes around the Bohai Sea in the late Quaternary, the foraminifera assemblages from a new borehole Lz908 in the southern coast of the Laizhou Bay were studied, and then the transgressive strata were indentified. Combined with accelerator mass spectrometry radiocarbon 14C（AMS14C） and optically stimulated luminescence（OSL） ages, the occurrence time of these transgressions were re-determined. The result showed that three major large-scale transgressions occurred separately at the beginning of marine isotopic stage 7（MIS7）, the last interglacial period（MIS5） and the Holocene. In addition, a small-scale transgression occurred in the mid-MIS6, and the corresponding transgressive stratum was deposited. The transgressive deposition of MIS3 was also discovered in this study. However, the characteristics of the foraminifera indicated the environment during this period was colder than that in the MIS5. By comparison with the global sea-level changes, the paleoenvironmental changes around the Bohai Sea in the late Quaternary can be consistent with the global climate changes.     

日本囊对虾Marsupenaeus japonicus)组织蛋白酶D基因克隆及表达分析

组织蛋白酶D是溶酶体天冬氨酸蛋白酶家族的主要成员,广泛参与动物机体细胞内蛋白质的降解过程,对维持细胞稳态和正常代谢具有重要的作用。为研究组织蛋白酶D在甲壳动物非特异性免疫和幼体发育过程中的作用,本研究采用RACE技术首次克隆得到日本囊对虾组织蛋白酶D基因cDNA序列,命名为MjCatD,其中开放阅读框长为1161 bp,编码386个氨基酸残基。序列分析和同源建模显示该基因编码的蛋白含有保守的N-糖基化位点、天冬氨酸蛋白酶签名序列、酶活化位点和非消化性组织蛋白酶D的特征序列,并且呈保守的双叶形结构。同源性比较和系统进化分析发现,MjCatD与斑节对虾、美洲螯龙虾和脊尾白虾相似性较高,并且与它们紧密聚为一支。实时荧光定量PCR结果显示,MjCatD基因在日本囊对虾多个组织中均有表达,其中肝胰腺中表达量最高。在白斑综合征病毒(white spot syndrome virus, WSSV)感染后3～24 h日本囊对虾肝胰腺中MjCatD的表达量逐渐下降,而在48 h急剧上调至最高表达量并且与对照组差异极显著(P<0.01)。此外,MjCatD基因在幼体发育不同阶段中也表现出明显的变化趋势。以上研究表明,MjCatD基因可能参与日本囊对虾先天免疫反应和幼体发育过程。     

Impact Compressive Creep Characteristics of Simulative Soil for Deep-Sea Sediment

The impact load (equivalent impact height) applied to deep-sea sediment by a walking mining machine was first deduced by the energy conservation principle, and the simulative soil was prepared based on the deep-sea sediment collected from the Pacific C-C mining area. The self-designed impact compressive creep tests of the simulative soil were conducted under different ground stresses and impact heights, in order to determine impact compressive creep parameters using a K-H rheological model. Test results show that the impact compressive creep curves have three similar creep stages (transient creep, unstable creep, and stable creep) to static compressive creep curves, where the transient creep deformation and total deformation at the unstable creep stage decrease with the impact load. Among the three impact compressive creep parameters (K₁, K₂, β) of the simulative soil, K₁ is first increased with impact height and finally fluctuated to a certain stable value, while K₂ and β are approximately linearly increased with impact height. The maximum subsidence of the mining machine under a specific designed ground stress and walking velocity predicted by the impact compressive creep constitutive equation can be used for safety assessment of the mining machine.     

Evaluation of Effective Depth of PVD Improvement in Soft Clay Deposit: A Field Case Study

This article presents a case history of determination of effective depth of prefabricated vertical drains (PVDs) under embankment loading on a very soft clay deposit in central China, near Jiujiang, Jiangxi Province. The height of the embankment was 5.3 m and construction time was about one year. The PVDs were installed to a depth of 8.5 m at a spacing of 1.5 m in a triangular pattern. Field observations and the finite element method (FEM) were employed to analyze the performance of the soft deposit during embankment construction. The influential depth of the embankment loading was evaluated based on settlement, excess pore pressure, and stress increase in subsoil, both from the observed data and FEM analysis. The effective PVD depth was determined in the following ways: (1) the depth of 5% subsoil settlement of surface settlement; (2) vertical stress increase in subsoil of 25% in-situ stress; and (3) consolidation time/PVD depth relation by FEM. Based on the analysis, the effective depth of PVDs was determined to be between 10 and 12.8 m for this field case.     

基于土地利用变化的中国省域碳排放时空差异及碳补偿研究

选择中国30个省级行政区为研究单元,基于土地利用和能源消耗等数据,采用碳排放系数法,对2003~2016年中国土地利用碳源/汇进行计算,探究中国省域土地利用碳排放和碳吸收时空演变,并以碳盈亏时空分析为基础,通过生态补偿系数和经济贡献系数分析碳排放的差异性,以净碳排放量作为基准值进行碳补偿价值的研究。结果表明:① 研究期间碳排放总量和净碳排放量除在2015年出现小幅度下降现象,其余年度均呈现不断增加趋势;碳吸收总量呈现稳中有升的趋势。②土地利用碳吸收的主要载体是耕地和林地,碳汇资源空间差异明显,基本呈现西高东低的分布特征,多数省份在研究期内基本保持一致的碳吸收类型。③ 碳排放总量分布空间差异显著,且主要来自建设用地。④ 依据净碳排放量的区域差异,将其划分为高碳排放区、一般碳排放区、低碳排放区、碳汇区4种类型。⑤ 由于各省域碳补偿标准和净碳排放量的差异导致碳补偿价值区域差异明显。