Uptake of palytoxin‐like compounds by shellfish fed Ostreopsis siamensis (Dinophyceae)

Abstract

Greenshell? mussels (Perna canaliculus Gmelin), scallops (Pecten novaezealandiae Reeve), and Pacific oysters (Crassostrea gigas Thunberg) were fed with a New Zealand strain of mass cultured Ostreopsis siamensis Schmidt (for 27 and 84 h and with 1.5 × 10⁶ or 8.6 × 10⁶ cells, respectively) under laboratory conditions. The microalgal cells contained 0.3 pg palytoxin equivalents cell^–1 (as determined by the haemolysis neutralisation assay (HNA) of Bignami (1993)) and extracts of these cells were toxic to mice after intraperitoneal injection. No palytoxin‐like material was detected either in the hepatopancreas or the muscle and roe of mussels fed O. siamensis. Oysters contained detectable amounts of toxin in hepatopancreas muscle, and roe while higher concentrations were present in the hepatopancreas of scallops. Extracts of control shellfish (tested biotoxin free and not fed O. siamensis) were toxic to mice, and there was no definitive evidence that feeding shellfish with O. siamensis at the levels employed in the present experiment increased the toxicity of shellfish tissue extracts to mice.     

Patch dynamics of soil biotic feedbacks in the Sonoran Desert

Soil fertility in arid ecosystems is spatially and temporally heterogeneous, due primarily to biotic feedbacks from perennial plants. While the “island of fertility” effect is well-established, little is known about the influence of plant functional characteristics on soil nutrients, or changes in nutrient levels following mortality or microsite succession. To assess these patterns, we studied a functionally diverse Sonoran Desert ecosystem in which all microsite state transitions were determined. The woody species (Ambrosia deltoidea, Larrea tridentata, Parkinsonia microphylla) significantly enhanced soil organic matter, nitrogen and phosphorous levels (referred to generally as fertility) to varying degrees. Effect sizes differed significantly among species but were surprisingly similar. However, following mortality, fertility remained constant beneath Ambrosia and increased significantly beneath decaying Parkinsonia, contrary to expected declines. These results suggest that nutrients are recycled within a microsite, resulting in similar pool sizes beneath live plants across species, regardless of morphological or phonological differences. Microsites occupied by cacti exhibited no differences from bare soil, indicating that cacti may erase islands of fertility. However, nitrate and phosphate levels spiked following mortality of the large cactus, Carnegiea gigantea. In general, fertility effects were correlated with morphological characteristics that should be applicable across most arid systems.     

Methodological Considerations Regarding the Measurement of Turbulent Fluxes in the Urban Roughness Sublayer: The Role of Scintillometery

We address some of the methodological challenges associated with the measurement of turbulence and use of scintillometers in the urban roughness sublayer (RSL). Two small-aperture scintillometers were located near the roof interface in a densely urbanized part of Basel, Switzerland, as part of the Basel Urban Boundary-Layer Experiment (BUBBLE) in the summer of 2002. Eddy correlation instruments were co-located near the mid-point of each scintillometer path for data verification purposes. The study presents the first values of the inner length scale of turbulence (l ₀) and the refractive index structure parameter of air for a city and demonstrates the influence of mechanical driven turbulence on dissipation. Comparison of dissipation values determined from the two approaches show large scatter that is possibly due to the spatial inhomogeneity of the turbulence statistics within the RSL. Velocity and temperature spectra display a −2/3 slope in the inertial subrange, although the spectral ratio is less than the theoretical prediction of 4/3 expected for isotropy. Conventional Monin–Obukhov equations used to calculate fluxes from the scintillometer were replaced with urban forms of the equations. The results suggest that the scintillometer may be an appropriate tool for the measurement of sensible heat flux (Q _H ) above the rooftops given a suitable determination of the effective measurement height.     

Change and variability in sea ice during the 2007–2008 Canadian International Polar Year program

In this paper we describe sea ice change and variability during the Canadian International Polar Year (IPY) program and examine several regional and hemispheric causes of this change. In a companion paper (Barber et al., Climate Change 2012) we present an overview of the consequences of this observed change and variability on ecosystem function, climatically relevant gas exchange, habitats of primary and apex predators, and impacts on northern peoples. Sea ice-themed research projects within the fourth IPY were designed to be among the most diverse international science programs. They greatly enhanced the exchange of Inuit knowledge and scientific ideas across nations and disciplines. This interdisciplinary and cultural exchange helped to explain and communicate the impacts of a transition of the Arctic Ocean and ecosystem to a seasonally ice-free state, the commensurate replacement of perennial with annual sea ice types and the causes and consequences of this globally significant metamorphosis. This paper presents a synthesis of scientific sea ice research and traditional knowledge results from Canadian-led IPY projects between 2007 and 2009. In particular, a summary of sea ice trends, basin-wide and regional, is presented in conjunction with Inuit knowledge of sea ice, gathered from communities in northern Canada. We focus on the recent observed changes in sea ice and discuss some of the causes of this change including atmospheric and oceanic forcing of both dynamic and thermodynamic forcing on the ice. Pertinent results include: 1) In the Amundsen Gulf, at the western end of the Northwest Passage, open water persists longer than normal and winter sea ice is thinner and more mobile. 2) Large areas of summer sea ice are becoming heavily decayed during summer and can be broken up by long-period waves being generated in the now extensive open water areas of the Chukchi Sea. 3) Cyclones play an important role in flaw leads??regions of open water between pack ice and land-fast ice. They delay the formation of new ice and the growth of multi-year ice. 4) Feedbacks involving the increased period of open water, long-period wave generation, increased open-ocean roughness, and the precipitation of autumn snow are all partially responsible for the observed reduction in multiyear sea ice. 5) The atmosphere is observed as remaining generally stable throughout the winter, preventing vertical entrainment of moisture above the surface.     

Simulating the diurnal cycle of rainfall in global climate models: resolution versus parameterization

The effects of horizontal resolution and the treatment of convection on simulation of the diurnal cycle of precipitation during boreal summer are analyzed in several innovative weather and climate model integrations. The simulations include: season-long integrations of the Non-hydrostatic Icosahedral Atmospheric Model (NICAM) with explicit clouds and convection; year-long integrations of the operational Integrated Forecast System (IFS) from the European Centre for Medium-range Weather Forecasts at three resolutions (125, 39 and 16 km); seasonal simulations of the same model at 10 km resolution; and seasonal simulations of the National Center for Atmospheric Research (NCAR) low-resolution climate model with and without an embedded two-dimensional cloud-resolving model in each grid box. NICAM with explicit convection simulates best the phase of the diurnal cycle, as well as many regional features such as rainfall triggered by advancing sea breezes or high topography. However, NICAM greatly overestimates mean rainfall and the magnitude of the diurnal cycle. Introduction of an embedded cloud model within the NCAR model significantly improves global statistics of the seasonal mean and diurnal cycle of rainfall, as well as many regional features. However, errors often remain larger than for the other higher-resolution models. Increasing resolution alone has little impact on the timing of daily rainfall in IFS with parameterized convection, yet the amplitude of the diurnal cycle does improve along with the representation of mean rainfall. Variations during the day in atmospheric prognostic fields appear quite similar among models, suggesting that the distinctive treatments of model physics account for the differences in representing the diurnal cycle of precipitation.     

Quantification of Storm-Induced Bathymetric Change in a Back-Barrier Estuary

Geomorphology is a fundamental control on ecological and economic function of estuaries. However, relative to open coasts, there has been little quantification of storm-induced bathymetric change in back-barrier estuaries. Vessel-based and airborne bathymetric mapping can cover large areas quickly, but change detection is difficult because measurement errors can be larger than the actual changes over the storm timescale. We quantified storm-induced bathymetric changes at several locations in Chincoteague Bay, Maryland/Virginia, over the August 2014 to July 2015 period using fixed, downward-looking altimeters and numerical modeling. At sand-dominated shoal sites, measurements showed storm-induced changes on the order of 5 cm, with variability related to stress magnitude and wind direction. Numerical modeling indicates that the predominantly northeasterly wind direction in the fall and winter promotes southwest-directed sediment transport, causing erosion of the northern face of sandy shoals; southwesterly winds in the spring and summer lead to the opposite trend. Our results suggest that storm-induced estuarine bathymetric change magnitudes are often smaller than those detectable with methods such as LiDAR. More precise fixed-sensor methods have the ability to elucidate the geomorphic processes responsible for modulating estuarine bathymetry on the event and seasonal timescale, but are limited spatially. Numerical modeling enables interpretation of broad-scale geomorphic processes and can be used to infer the long-term trajectory of estuarine bathymetric change due to episodic events, when informed by fixed-sensor methods.     

Predicting phosphorus dynamics in complex terrains using a variable source area hydrology model

Phosphorus (P) loss from agricultural watersheds has long been a critical water quality problem, the control of which has been the focus of considerable research and investment. Preventing P loss depends on accurately representing the hydrological and chemical processes governing P mobilization and transport. The Soil and Water Assessment Tool (SWAT) is a watershed model commonly used to predict run‐off and non‐point source pollution transport. SWAT simulates run‐off employing either the curve number (CN) or the Green and Ampt methods, both assume infiltration‐excess run‐off, although shallow soils underlain by a restricting layer commonly generate saturation‐excess run‐off from variable source areas (VSA). In this study, we compared traditional SWAT with a re‐conceptualized version, SWAT‐VSA, that represents VSA hydrology, in a complex agricultural watershed in east central Pennsylvania. The objectives of this research were to provide further evidence of SWAT‐VSA's integrated and distributed predictive capabilities against measured surface run‐off and stream P loads and to highlight the model's ability to drive sub‐field management of P. Thus, we relied on a detailed field management database to parameterize the models. SWAT and SWAT‐VSA predicted discharge similarly well (daily Nash–Sutcliffe efficiencies of 0.61 and 0.66, respectively), but SWAT‐VSA outperformed SWAT in predicting P export from the watershed. SWAT estimated lower P loss (0.0–0.25 kg ha^?1) from agricultural fields than SWAT‐VSA (0.0–1.0+ kg ha^?1), which also identified critical source areas – those areas generating large run‐off and P losses at the sub‐field level. These results support the use of SWAT‐VSA in predicting watershed‐scale P losses and identifying critical source areas of P loss in landscapes with VSA hydrology. Copyright © 2014 John Wiley & Sons, Ltd.