Phytoplankton pigments and epifluorescence microscopy as tools for ecological status assessment in coastal and estuarine waters, within the Water Framework Directive

Inverted microscopy is widespread employed for the analysis of phytoplankton composition within water quality monitoring networks. However, the analysis at the lowest taxonomical level is not always required for ecological status assessment. In addition, inverted microscopy can underestimate the small phytoplankton, and not always distinguish photoautotrophic from heterotrophic cells. In this study, as alternative tools, epifluorescence microscopy and High Performance Liquid Chromatography (HPLC) were employed to characterize phytoplankton communities within waters of different trophic condition. Epifluorescence microscopy confirmed its effectiveness to count the small phytoplankton. Furthermore, significant correlations between nutrients of anthropogenic origin and nanoplankton abundances were found. However, this technique resulted very time-consuming. HPLC together with the CHEMTAX program was more appropriate than inverted microscopy, in terms of cost-effectiveness. Also, the main variability patterns observed in the phytoplankton community structure by HPLC coincided with previous findings in the study area. Nevertheless, a rapid screening at the inverted microscope is recommended.     

Spartina densiflora demonstrates high tolerance to phenanthrene in soil and reduces it concentration

The present study was conducted to investigate the tolerance of Spartina densiflora to phenanthrene, and to test its ability in phenanthrene dissipation. A glasshouse experiment was designed to investigate the effect of phenanthrene from 0 to 1000 mg kg⁻¹ on growth and photosynthetic apparatus of S. densiflora by measuring chlorophyll fluorescence parameters, gas exchange and photosynthetic pigments. We also performed chemical analysis of plant samples, and determined the concentration of phenanthrene remaining in soil. S. densiflora survived to concentrations as high as 1000 mg kg⁻¹ phenanthrene in soil; in fact, there was no significant difference in RGR among the treatments after 30 days. Otherwise, phenanthrene affected photosynthetic apparatus at 100 and 1000 mg kg⁻¹; thus, the lower Φ_PSII could be explained by the declined photosynthetic pigment concentrations. Soil extraction indicated a more marked rate of phenanthrene disappearance in the soil in the presence of S. densiflora.     

Mineralogy,diagenesis and hydrochemical evolution in a probertite–glauberite–halite saline lake (Miocene,Emet Basin,Turkey)

The Emet basin is one of the Neogene basins in western Turkey containing significant amounts of borate minerals, mainly colemanite. The petrologic study of core samples from two exploratory wells in the Do?anlar sector, under optic and electron microscopy, reveals a complex mineral association in which probertite, glauberite, and halite constitute the major primary phases (without mineral precursors) precipitated in a saline lake placed in a volcano-sedimentary context. Other sulfates (anhydrite, gypsum, thenardite, celestite and kalistrontite), borates (colemanite, ulexite, hydroboracite, tunellite, kaliborite and aristarainite), and sulfides (arsenopyrite, realgar and orpiment) are attributed to early diagenesis. So far, the Do?anlar deposit is the most important deposit of probertite known up to now.Chemical changes in the groundwater inflow led to the precipitation of Ca-bearing borates (colemanite) in the tuff-flat environment surrounding the lake, while Na–Ca sulfates and borates (glauberite and probertite) precipitated in the center of the lake. Fluid inclusion compositions in halite indicate that the advanced brines correspond to the Na-K-Cl-SO₄ type. During restricted stages of the saline lake, the residual brines seeped through the tuff-flat sediments, leading to transformations of previous precipitates that resulted in the formation of K-bearing minerals.The abundance of coccoid-like biogenic dolomite, colloidal arsenopyrite and the isotopic composition of sulfates are indicative of bacterial sulfate reduction. In contrast, arsenic sulfides are attributed to acidophilic micro-organisms in oxidizing conditions. Fluctuations of redox conditions in both free and interstitial brines control the biological influence in some diagenetic transformations.     

Decadal predictability and prediction skill of sea surface temperatures in the South Pacific region

The South Pacific Ocean is a key driver of climate variability within the Southern Hemisphere at different time scales. Previous studies have characterized the main mode of interannual sea surface temperature (SST) variability in that region as a dipolar pattern of SST anomalies that cover subtropical and extratropical latitudes (the South Pacific Ocean Dipole, or SPOD), which is related to precipitation and temperature anomalies over several regions throughout the Southern Hemisphere. Using that relationship and the reported low predictive skill of precipitation anomalies over the Southern Hemisphere, this work explores the predictability and prediction skill of the SPOD in near-term climate hindcasts using a set of state-of-the-art forecast systems. Results show that predictability greatly benefits from initializing the hindcasts beyond the prescribed radiative forcing, and is modulated by known modes of climate variability, namely El Niño-Southern Oscillation and the Interdecadal Pacific Oscillation. Furthermore, the models are capable of simulating the spatial pattern of the observed SPOD even without initialization, which suggests that the key dynamical processes are properly represented. However, the hindcast of the actual phase of the mode is only achieved when the forecast systems are initialized, pointing at SPOD variability to not be radiatively forced but probably internally generated. The comparison with the performance of an empirical prediction based on persistence suggests that initialization may provide skillful information for SST anomalies, outperforming damping processes, up to 2–3 years into the future.     

Spatial variation of biological and pedological properties in an area affected by a metallurgical mercury plant: Almadenejos (Spain)

Almadén, Spain, has the most important known cinnabar deposits, which have provided a third of the entire world production of Hg, and has been the scene for numerous studies on Hg. This mining district includes several mines where cinnabar has been extracted in the past two millennia as well as facilities related to the production of primary Hg. The aim of the work is to evaluate the spatial distribution of Hg in the soil-plant system within an area where intense activity occurred over a long period. An abandoned metallurgical plant from the 17th-18th centuries was chosen as the study area, situated in Almadenejos a distance of 12 km from Almadén. Nowadays, this plot is covered with cinnabar mine tailings and it is used by village inhabitants for livestock grazing. The area has elevated Hg concentrations of natural origin and from human activities. Soil parameters are similar within the study area; however, data reveal high variability in total and available Hg concentrations in soils, making it difficult to establish a trend. Marrubium vulgare L. has been studied due to its abundance in the plot, and there is no evidence of phenological toxicity. In spite of elevated Hg concentrations, high biological activity is found in the sampled soils. All these characteristics, spatial variation, high Hg concentration, good biological activity, make this a particularly good area for studies involving Hg.     

Interplay of phosphogenesis and hydrothermalism in the latest Ediacaran rift of the High Atlas,Morocco

Two dissolution surfaces, capped by phosphatic crusts that contain up to 40 wt.% of P₂O₅, are well exposed in the latest Ediacaran Tabia Member (Adoudou Formation) of the Ouneïn region, High Atlas. Both the Tabia dolostones and capping phosphorites are crosscut by several generations of synsedimentary faults, fissures, and hydrothermal dikes hosting Cu ore bodies, in a scenario of down-faulted blocks. Stratigraphic and facies relationships suggest the preservation of a major pulse of intra-cratonic rifting, responsible for the development of an unstable carbonate platform subjected to sharp uplift and tilting perturbations and dissolution processes, associated with hydrothermal dike-swarm injection. The whole framework is sealed with the Tamjout Bed (Tifnout Member), which marks the end of abundant regional tilting (syn-rift phase) and the beginning of a thermal subsidence-dominated regime (post-rift phase) in the Moroccan margin of West Gondwana.     

Seasonal changes and morphodynamic behavior of a high-energy mesotidal beach: case study of Charf el Akab beach on the North Atlantic coast of Morocco

The morphodynamic behavior of a mesotidal sandy beach was monitored during both calm and energetic conditions. Two years of seasonal surveys were carried out on Charf el Akab, a gently sloped beach in the North Atlantic coast of Morocco. The method of survey consisted of a 3D study of the beach morphological changes and provided 2 cm vertical accuracy. During the surveyed period, Charf el Akab beach underwent very energetic wave conditions, and the breaking wave height was of H _b ≥ 1.5 m. The beach is characterized by a nonpermanent swash bar and composed of well-sorted medium sand. The application of environmental parameters revealed a dissipative state with very low beach gradient which did not vary significantly over the studied period. Morphological changes consist of beach erosion and bar decay under high-energy waves, whereas the intertidal bar re-established and the beach recorded an accentuated accretion due to relatively fair weather conditions. The beach volume reveals a seasonal behavior; the sand accumulated during summer is dramatically removed during winter season. The range in beach sand volume from the most accreted to the most eroded conditions observed is about −5,493 m³. The average sand volume flux between surveys reaches −1 and 0.4 m²/day during peak erosion and accretion periods. The relationships between the wave forcing and the sand volume adjustments were examined. The sand volume change was found to be highly correlated (0.91) with the wave energy flux. The highest correspondence (0.95) was found between the sand flux rate and the wave energy flux. The wave forcing is expected to be the main factor governing beach morphodynamics at Charf el Akab site.     

Reconstruction of Late-Quaternary sea- and lake-level changes in a tectonically active marginal basin using seismic stratigraphy: The Gulf of Cariaco,NE Venezuela

The Gulf of Cariaco is a marginal basin located between the Cariaco Basin and the Paria Gulf, offshore NE Venezuela, along a system of active right-lateral strike-slip faults. It is connected to the Caribbean Sea via a shallow 58-m-deep sill implying that the gulf was disconnected from the global ocean during eustatic lowstands. A dense grid of high-resolution reflection seismic profiles has been used to determine the overall tectonic structure of the gulf and to establish the seismic stratigraphy of its sedimentary infill. Six unconformity-bounded seismic–stratigraphic units were identified in the upper ~ 200 m of the sedimentary infill. Detailed seismic–stratigraphic and seismic-facies analysis allowed defining a series of sedimentary features that can be used as indicators of past sea or lake level in the Gulf of Cariaco: i) delta offlap breaks, ii) evaporites, and iii) erosional unconformities. Using accurate measurements of these various indicators at several locations in the gulf and a simple total subsidence model, a relative sea/lake-level history encompassing the last 130 kyr could be reconstructed. In periods of connection with the open ocean, reconstructed relative sea level correlates well with eustatic sea level. In times of disconnection, distinct lake-level fluctuations occurred, which sometimes resulted in total dessication of the gulf. Lake-level fluctuations appear to correlate with major Heinrich Events, stadials and interstadials. MIS 4, the LGM and the Younger Dryas were thus identified in the Gulf of Cariaco sedimentary record. The last reconnection to the Caribbean Sea occurred during MWP1b (around 11.5 kyr). The very good fit of the Cariaco sea/lake-level curve with the eustatic sea-level curves (both in terms of amplitude and of timing) underscores potential for future paleoclimate research of the sedimentary record contained in this marginal basin, despite its active tectonic setting.     

Contourites within a deep-water sequence stratigraphic framework

Sequence stratigraphy has proven to be an extremely useful predictive tool in the search for hydrocarbons along the continental margins. However, of the several models in use, none includes the effects of alongslope processes in deep-water. This paper, therefore, is a first attempt to place contourite depositional systems (CDSs) firmly within a sequence stratigraphic framework, based on detailed examination of over 20 CDSs worldwide. It also presents a new view of how sea level variation influences bottom current generation and intensity. Two key controls on contourite drift formation are identified: sediment influx and bottom current velocity. Sea level directly influences the sediment influx to a basin and, therefore, the contourite response fits nicely into the downslope sequence stratigraphy model. Bottom current velocity variations in response to sea level are more complex, and two key controls are identified: (1) oceanic gateways can effectively constrict and accelerate water masses and are therefore closely associated with CDS evolution; fluctuating sea level will affect the water exchange through a gateway; (2) changing rates of bottom-water generation: some water masses appear more vigorous during periods of lowstand, whereas others appear more sluggish. In order to accommodate this variation, two new sequence stratigraphy models are herein presented, comprising both downslope and alongslope processes. The first model reveals a CDS where bottom current activity is markedly more vigorous during times of sea level highstand, whereas the second model indicates margin evolution where bottom-water currents are most vigorous during times of sea level lowstand. It is recognised that there are additional controlling factors linked to sea level variation which can significantly modify the distribution and development of contourite elements.