Biostimulation and Bioaugmentation to Enhance Reductive Dechlorination of TCE in a Long‐Term Flow Through Column Study

Large laboratory columns (15.2 cm diameter, 183 cm long) were fed with groundwater containing trichloroethylene (TCE), were biostimulated and bioaugmented, and were monitored for over 7.5 years. The objective of the study was to observe how the selection of the carbon and energy source, i.e., whey, Newman Zone^® standard surfactant emulsified oil and Newman Zone nonionic surfactant emulsified oil, affected the rate and extent of dechlorination. Column effluent was monitored for TCE and its degradation products, redox indicators (nitrate‐N, Fe(II), sulfate), and changes in iron mineralogy. Total bacteria and Dehalococcoides mccartyi strains were quantified using q‐PCR. Complete dechlorination was only observed in the whey treated columns, occurring 1 year after bioaugmentation with addition of a culture known to dechlorinate TCE to ethene, and 3 years later in the non‐bioaugmented column. The addition of the emulsified oils with or without bioaugmentation resulted in dechlorination only through cis‐DCE and vinyl chloride. While Dehalococcoides mccartyi strains are the only known bacteria that can fully dechlorinate TCE, their presence, either natural or augmented, was not the sole determiner of complete dechlorination. The establishment of a supporting microbial community and biogeochemistry that developed with continuous feeding of whey, in addition to the presence of D. mccartyi, were necessary to support complete reductive dechlorination. Results confirm that careful selection of a biostimulant is critical to the success of TCE dechlorination in complex soil environments.     

Cloning a new cytochrome P450 isoform (CYP356A1) from oyster Crassostrea gigas

We have cloned the full-length cDNA of the first member of a new cytochrome P450 (CYP) family from the Pacific oyster Crassostrea gigas. This new CYP gene was obtained based on an initial 331bp fragment previously identified among the list of the differentially expressed genes in oysters exposed to untreated domestic sewage. The full-length CYP has an open reading frame of 1500bp and based on its deduced amino acid sequence was classified as a member of a new subfamily, CYP356A1. A phylogenetic analysis showed that CYP356A1 is closely related to members of the CYP17 and CYP1 subfamilies. Semi-quantitative RT-PCR was performed to analyze the CYP356A1 expression in different tissues of the oyster (digestive gland, gill, mantle and adductor muscle). Results showed slightly higher CYP356A1 expression in digestive gland and mantle, than the other tissues, indicating a possible role of the CYP356A1 in xenobiotic biotransformation and/or steroid metabolism.     

A Forward Modeling Approach for Interpreting Impeller Flow Logs

A rigorous and practical approach for interpretation of impeller flow log data to determine vertical variations in hydraulic conductivity is presented and applied to two well logs from a Chalk aquifer in England. Impeller flow logging involves measuring vertical flow speed in a pumped well and using changes in flow with depth to infer the locations and magnitudes of inflows into the well. However, the measured flow logs are typically noisy, which leads to spurious hydraulic conductivity values where simplistic interpretation approaches are applied. In this study, a new method for interpretation is presented, which first defines a series of physical models for hydraulic conductivity variation with depth and then fits the models to the data, using a regression technique. Some of the models will be rejected as they are physically unrealistic. The best model is then selected from the remaining models using a maximum likelihood approach. This balances model complexity against fit, for example, using Akaike's Information Criterion.     

Radar absorption due to a corotating interaction region encounter with Mars detected by MARSIS

Mars Advanced Radar for Subsurface and Ionospheric Sounding (MARSIS) is a subsurface and topside ionosphere radar sounder aboard the European Space Agency spacecraft Mars Express, in orbit at Mars since 25 December 2003, and in operation since 17 June 2005. The ionospheric sounding mode of MARSIS is capable of detecting the reflection of the sounding wave from the martian surface. This ability has been used in previous work to show that the surface reflection is absorbed and disappears during periods when high fluxes of energetic particles are incident on the ionosphere of Mars. These absorption events are believed to be the result of increased collisional damping of the sounding wave, caused by increased electron density below the spacecraft, in turn caused by impact ionization from the impinging particles. In this work we identify two absorption events that were isolated during periods when the surface reflection is consistently visible and when Mars is nearly at opposition. The visibility of the surface reflection is viewed in conjunction with particle and photon measurements taken at both Mars and Earth. Both absorption events are found to coincide with Earth passing through solar wind speed and ion flux signatures indicative of a corotating interaction region (CIR). The two events are separated by an interval of approximately 27 days, corresponding to one solar rotation. The first of the two events coincides with abruptly enhanced particle fluxes seen in situ at Mars. Simultaneous with the particle enhancement there are an abrupt decrease in the intensity of electron oscillations, typically seen by the Mars Express particle instrument ASPERA-3 between the magnetic pileup boundary and the martian bow shock, and a sharp drop in the solar wind pressure, seen in the proxy quantity based on MGS magnetometer observations. The decrease in oscillation intensity is therefore the probable effect of a relaxation of the martian bow shock. The second absorption event does not show a particle enhancement and complete ASPERA-3 data during that time are unavailable. Other absorption events are the apparent result of solar X-ray and XUV enhancements. We conclude that surface reflection absorption events are sometimes caused by enhanced ionospheric ionization from high energy particles accelerated by the shocks associated with a CIR. A full statistical analysis of CIRs in relation to observed absorption events in conjunction with a quantitative analysis of the deposition of ionization during space weather events is needed for a complete understanding of this phenomenon. If such analyses can be carried out, radar sensing of the martian ionosphere might be useful as a space weather probe.     

Systematic study of universal‐stage measurements of planar deformation features in shocked quartz: Implications for statistical significance and representation of results

Abstract— The presence of shocked quartz is one of the key lines of evidence for the impact origin of rocks. Crystallographic orientations of planar deformation feature (PDF) sets in shocked quartz have been used to constrain the peak shock pressure that these grains have experienced. So far no systematic and comparative studies of the various orientation measurement methods and their biases are available. Therefore, three shocked‐quartz‐bearing thin sections from a meta‐greywacke clast in breccia, a biotite‐gneiss, and a sandstone, respectively, were independently analyzed by three operators (two experienced and one inexperienced) using a four‐axis universal‐stage (U‐stage), in order to evaluate the quality, precision, repeatability, and representativeness of U‐stage measurements. Based on the indexing of PDF sets using a new version of the commonly used stereographic projection template, the study of 1751 PDF set orientations in 666 quartz grains in three different shocked rocks shows that differences in abundance and orientation of various PDF sets, as measured by the three separate operators, are rather limited. The precision of U‐stage measurements depends mainly on the number of PDF sets investigated, as the ability level of the operator (experienced versus inexperienced) is only responsible for minor deviations in the number of unindexed planes. The frequency percent of dominant PDF planes may vary by up to 20 percentage points (pp) or 81% for a given crystallographic orientation when only 25 sets are measured. When 100 PDF sets are measured, however, this deviation in dominant orientations is reduced to about 7 pp or 28%. We recommend the use of a new stereographic projection template, which plots the pole positions of five additional, commonly occurring PDF orientations, as it can allow indexing of up to 12 pp more PDF planes; these are planes that would previously be considered unindexed and potentially regarded as errors of measurement. Our results suggest that by following a strict measurement procedure, the reproducibility of U‐stage measurements is good and the results of different studies can be readily compared. However, it is critical that published PDF orientation histograms clearly define what type of frequency measurement is used, whether or not unindexed PDF sets are included in the frequency calculations, the numbers of grains and sets analyzed, and the relative proportions of each PDF set population that are combined in the histograms. This information appears to be essential for effectively comparing datasets from different studies.     

Sedimentary and foraminiferal records of Late Quaternary environmental change west of Ireland and implications for the last British–Irish Ice Sheet

Palaeoenvironmental change following deglaciation of the last British–Irish Ice Sheet on the continental shelf west of Ireland was investigated using multiproxy analyses of sediment and foraminifera data from nine sediment cores. Lithofacies associations record various depositional regimes across the shelf, which evolve from subglacial to postglacial conditions. Census data provide the first characterisation of benthic foraminifera populations across the continental shelf and multivariate analyses reveal three distinct biotopes. Biomineralization within these biotopes is restricted to ≤21 200 cal a bp by four radiocarbon ages. The transition from glacial to postglacial benthic foraminifera populations near the shelf break marks the establishment of productive, nutrient-rich, ice-distal conditions at ~20 900 cal a bp ; these conditions may also mark the start of favourable conditions for postglacial cold-water coral growth. Postglacial conditions on the inner shelf were not established until <14 500 cal a bp , suggesting glacial conditions west of Ireland may have persisted into the Bølling–Allerød Interstadial.     

Multi-criteria spatial screening and uncertainty analysis applied to direct-use geothermal projects

ABSTRACT

The focus of this work is general methods for prioritization or screening of project sites based on the favorability of multiple spatial criteria. We present a threshold-based transformation of each underlying spatial favorability factor into a continuous scale with a common favorability interpretation across all criteria. We compare several methods of computing site favorability and propagating uncertainty from the data to the favorability metrics. Including uncertainty allows decision makers to determine if seeming differences among sites are significant. We address uncertainty using Taylor series approximations and analytical distributions, which are compared to computationally intensive Monte Carlo simulations. Our methods are applied to siting direct-use geothermal energy projects in the Appalachian Basin, where our knowledge about any particular site is limited, yet sufficient data exist to estimate favorability. We consider four factors that contribute to site favorability: the thermal resource described by the depth to 80°C rock, natural reservoir productivity described by rock permeability and thickness, potential for induced seismicity, and the estimated cost of surface infrastructure for heat distribution. Those factors are combined in three ways. We develop favorability uncertainty propagation and sensitivity analysis methods. All methods are general and can be applied to other multi-criteria spatial screening problems.