Tropical Marine Fungi*

Abstract. Marine Ascomycetes, Basidiomycetes and Deuteromycetes were collected in tropical and subtropical regions (Australia, Belize, Fiji, Hawaii, Marshall Islands, Mexico, New Zealand, Palau, Thailand), and the known range of distribution for these fungi was extended. Exclusively tropical are 27 taxa, 9 are probably restricted to the tropics also, and 11 are cosmopolitan. Distribution maps are given for 5 taxa. New species (4), varieties (3), and combinations (2) of Ascomycetes are proposed, and keys to the taxa of Halosarpheia and Lulworthia are presented. Most of the species are decomposers of mangrove parts or of detritus in sandy beaches. Nine new host plants were found. Marine Ascomycetes were discovered for the first time living in shells of foraminifera.     

Mercury cycling during acid rain recovery at the forested Lesní potok catchment,Czech Republic

From 2011 to 2019, mercury (Hg) stores and fluxes were studied in the small forested catchment Lesní potok (LES) in the central Czech Republic using the watershed mass balance approach together with internal measurements. Mean input fluxes of Hg via open bulk deposition, beech throughfall and spruce throughfall during the periodwere 2.9, 3.9 and 7.6 μg m⁻² year⁻¹, respectively. These values were considerably lower than corresponding deposition Hg fluxes reported in the early years of the 21st century from catchments in Germany. Current bulk precipitation inputs at unimpacted Czech mountainous sites were lower than those in Germany. The largest Hg inputs to the catchment were via litterfall, averaging 22.6 and 17.8 μg m⁻² year⁻¹ for beech and spruce stands. The average Hg input, based on the sum of mean litterfall and throughfall deposition, was 23.0 μg m⁻² year⁻¹, compared to the estimated Hg output in runoff of 0.5 μg m⁻² year⁻¹, which is low compared to other reported values. Thus, only ~2% of Hg input is exported in stream runoff. Stream water Hg was only weakly related to dissolved organic carbon (DOC) but both concentrations were positively correlated with water temperature. The estimated total soil Hg pool averaged 47.5 mg m⁻², only 4% of which was in the O-horizon. Thus Hg in the O-horizon pool represents 72 years of deposition at the current input flux and 3800 years of export at the current runoff flux. Age-dating by ¹⁴C suggested that organic soil contains Hg from recent deposition, while mineral soil at 40–80 cm depth contained 4400-year old carbon, suggesting the soil had accumulated atmospheric Hg inputs through millennia to reach the highest soil Hg pool of the soil profile. These findings suggest that industrial era intensification of the Hg cycle is superimposed on a slower-paced Hg cycle during most of the Holocene.     

Generic 3D interpolation of Holocene base‐level rise and provision of accommodation space,developed for the Netherlands coastal plain and infilled palaeovalleys

We present an interpolation model that describes Holocene groundwater level rise and the creation of accommodation space in 3D in the Rhine‐Meuse delta – the Netherlands. The model area (ca. 12 400 km²) covers two palaeovalleys of Late Pleistocene age (each 30 km wide) and the overlying Holocene deposits of the Rhine‐Meuse delta, the Holland coastal plain, and the Zuiderzee former lagoon. Water table rise is modelled from 10 800 to 1000 cal. BP, making use of age‐depth relations based on 384 basal peat index points, and producing output in the form of stacked palaeo groundwater surfaces, groundwater age‐depth curves, and voxel sets. These products allow to resolve (i) regional change and variations of inland water table slopes, (ii) spatial differences in the timing and pacing of transgression, and (iii) analysis of interplay of coastal, fluvial and subsidence controls on the provision of accommodation space. The interpolation model is a multi‐parameter trend function, to which a 3D‐kriging procedure of the residuals is added. This split design deploys a generic approach for modelling provision of accommodation space in deltas and coastal lowlands, aiming to work both in areas of intermediate data availability and in the most data‐rich environments. Major provision of accommodation space occurred from 8500 cal BP onwards, but a different evolution occurred in each of the two palaeovalleys. In the northern valley, creation of accommodation space began to stall at 7500 cal BP, while in the southern valley provision of new accommodation space in considerable quantities continued longer. The latter is due to the floodplain gradient that was maintained by the Rhine, which distinguishes the fluvial deltaic environment from the rest of the back‐barrier coastal plain. The interpolation results allow advanced mapping and investigation of apparent spatial differences in Holocene aggradation in larger coastal sedimentary systems. Furthermore, they provide a means to generate first‐order age information with centennial precision for 3D geological subsurface models of Holocene deltas and valley fills. As such, the interpolation is of use in studies into past and present land subsidence and into low land sedimentation.     

Reformulated O correction of mass spectrometric stable isotope measurements in carbon dioxide and a critical appraisal of historic ‘absolute’ carbon and oxygen isotope ratios

Mass-spectrometric stable isotope measurements of CO₂ use molecular ion currents at mass-to-charge ratios m/z 44, 45 and 46 to derive the elemental isotope ratios n(¹³C)/n(¹²C) and n(¹⁸O)/n(¹⁶O), abbreviated ¹³C/¹²C and ¹⁸O/¹⁶O, relative to a reference. The ion currents have to be corrected for the contribution of ¹⁷O-bearing isotopologues, the so-called ‘¹⁷O correction’. The magnitude of this correction depends on the calibrated isotope ratios of the reference. Isotope ratio calibrations are difficult and are therefore a matter of debate. Here, I provide a comprehensive evaluation of the existing ¹³C/¹²C (¹³R), ¹⁷O/¹⁶O (¹⁷R) and ¹⁸O/¹⁶O (¹⁸R) calibrations of the reference material Vienna Standard Mean Ocean Water (VSMOW) and CO₂ generated from the reference material Vienna Pee Dee Belemnite (VPDB) by reaction with 100% H₃PO₄ at 25 °C (VPDB-CO₂). I find , ¹⁸R_VSMOW/10⁻⁶ = 2005.20 ± 0.45, ¹³R_VPDB-CO2/10^-6= 11124 ± 45, and ¹⁸R_VPDB-CO2/10^-6=2088.37±0.90. I also rephrase the calculation scheme for the ¹⁷O correction completely in terms of relative isotope ratio differences (δ values). This reveals that only ratios of isotope ratios (namely, ¹⁷R/¹³R and ¹³R¹⁷R/¹⁸R) are required for the ¹⁷O correction. These can be, and have been, measured on conventional stable isotope mass spectrometers. I then show that the remaining error for these ratios of isotope ratios can lead to significant uncertainty in the derived relative ¹³C/¹²C difference, but not for¹⁸O/¹⁶O. Even though inter-laboratory differences can be corrected for by a common ‘ratio assumption set’ and/or normalisation, the ultimate accuracy of the ¹⁷O correction is hereby limited. Errors of similar magnitude can be introduced by the assumed mass-dependent relationship between ¹⁷O/¹⁶O and ¹⁸O/¹⁶O isotope ratios. For highest accuracy in the ¹³C/¹²C ratio, independent triple oxygen isotope measurements are required. Finally, I propose an experiment that allows direct measurement of ¹³R¹⁷R/¹⁸R.     

An integrated geophysical study of Vestbakken Volcanic Province, western Barents Sea continental margin, and adjacent oceanic crust

This paper describes results from a geophysical study in the Vestbakken Volcanic Province, located on the central parts of the western Barents Sea continental margin, and adjacent oceanic crust in the Norwegian-Greenland Sea. The results are derived mainly from interpretation and modeling of multichannel seismic, ocean bottom seismometer and land station data along a regional seismic profile. The resulting model shows oceanic crust in the western parts of the profile. This crust is buried by a thick Cenozoic sedimentary package. Low velocities in the bottom of this package indicate overpressure. The igneous oceanic crust shows an average thickness of 7.2 km with the thinnest crust (5–6 km) in the southwest and the thickest crust (8–9 km) close to the continent-ocean boundary (COB). The thick oceanic crust is probably related to high mantle temperatures formed by brittle weakening and shear heating along a shear system prior to continental breakup. The COB is interpreted in the central parts of the profile where the velocity structure and Bouguer anomalies change significantly. East of the COB Moho depths increase while the vertical velocity gradient decreases. Below the assumed center for Early Eocene volcanic activity the model shows increased velocities in the crust. These increased crustal velocities are interpreted to represent Early Eocene mafic feeder dykes. East of the zone of volcanoes velocities in the crust decrease and sedimentary velocities are observed at depths of more than 10 km. The amount of crustal intrusions is much lower in this area than farther west. East of the Kn?legga Fault crystalline basement velocities are brought close to the seabed. This fault marks the eastern limit of thick Cenozoic and Mesozoic packages on central parts of the western Barents Sea continental margin.     

Parametric rolling behaviour of azimuthing propulsion-driven ships

The study investigates the experimental and numerical analysis of the occurrence of auto-parametric rolling for large, high-speed pod-driven ships in waves. Considering unique design and performance targets, the aim here is to exploit susceptibility to auto-parametric rolling behaviour and to identify probable design and operational precautions. In order to achieve this aim, an existing non-linear time-domain software to simulate capsizing and other critical manoeuvring behaviours of slow- to medium-speed conventional and podded ships in waves is being enhanced for fast pod-driven vessels and then compared against the dedicated model test conducted in long-crested regular and random waves for a large, pod-driven containership model. This paper includes the presentation of current numerical modifications for pod-driven ships and the verification analysis.     

Automated mapping of rock slope geometry,kinematics and stability with RSS-GIS

A GIS-implemented, deterministic approach for the automated spatial evaluation of geometrical and kinematical properties of rock slope terrains is presented. Based on spatially distributed directional information on planar geological fabrics and DEM-derived topographic attribute data, the internal geometry of rock slopes can be characterized on a grid cell basis. For such computations, different approaches for the analysis and regionalization of available structural directional information applicable in specific tectonic settings are demonstrated and implemented in a GIS environment. Simple kinematical testing procedures based on feasibility criteria can be conducted on a pixel basis to determine which failure mechanisms are likely to occur at particular terrain locations. In combination with hydraulic and strength data on geological discontinuities, scenario-based rock slope stability evaluations can be performed. For conceptual investigations on rock slope failure processes, a GIS-based specification tool for a 2-D distinct element code (UDEC) was designed to operate with the GIS-encoded spatially distributed rock slope data. The concepts of the proposed methodology for rock slope hazard assessments are demonstrated at three different test sites in Germany.     

Effects of cadmium, zinc and nitrogen status on non-protein thiols in the macroalgae Enteromorpha spp. from the Scheldt Estuary (SW Netherlands, Belgium) and Thermaikos Gulf (N Aegean Sea, Greece)

Enteromorpha prolifera (Scheldt Estuary) and E. linza (Thermaikos Gulf) were incubated at three salinities with 100 and 200microgL(-1)Cd and Zn. The objective was to measure effects of Cd, Zn and nitrogen (N) status on the pools of metal-binding non-protein thiols: glutathione and phytochelatins, (gamma-glutamyl-cysteinyl)(n)-glycine (PC). In E. linza, ammonium pools were higher, but amino acid pools, total N and protein contents were lower than in E. prolifera. Reduced glutathione (GSH) pools were positively correlated with free glutamate and protein contents. In E. linza GSH pools increased and the ratio of reduced to oxidized glutathione (GSH:(GSH+0.5GSSG)), an indicator of oxidative stress, decreased with Cd contents, indicating Cd-induced glutathione oxidation. Total glutathione pools (reduced plus oxidized) ranged from 16nmolSgdwt(-1) in controls (at 0.5micromolCdgdwt(-1)) to 179nmolSgdwt(-1) (at 1.9micromolCdgdwt(-1)) at the highest cadmium dosage. Cadmium stimulated PC synthesis in E. prolifera which suggests that in N-rich algae, glutathione pools were high enough for PC synthesis. In both species GSH and protein increased with Zn contents, whereas GSH:(GSH+0.5GSSG) decreased, which would indicate Zn-induced oxidative stress; in E. linza, at the highest salinity the glutathione redox ratio decreased from 0.61 (at 2.9micromolZngdwt(-1)) to 0.26 (at 4.9nmolSgdwt(-1)) (at 0.5molCdgdwt(-1)). PCs were not synthesized in response to Zn, which may have resulted in Zn-induced GSH oxidation. The presence of both oxidative effects (Cd, Zn) and detoxification (Cd) could be identified by observing the responses of glutathione and PC pools to metal stress.     

Evaluation of the Presence of Methane in Złoczew Lignite: Comparison with Other Lignite Deposits in Poland

Natural Resources Research - Catastrophic cases of methane explosion during exploratory drilling within the Be?chatów ortholignite deposit have led to testing for methane in other Polish...