Histopathology alterations and histochemistry measurements in mussel, Mytilus edulis collected offshore from an aluminium smelter industry (Norway)

Histopathological characteristics of specific organs express condition, and represent time-integrated impacts on the organism stemming from alterations at lower levels of biological organisation. As integrative parameters, histochemical investigations have proved to be sensitive tools to detect effects of chemical compounds. The objective of this study was to determine changes in the tissues of mussels collected at a PAH contaminated site compared to a reference site using histopathological and histochemical parameters: lipofuscin (LF) accumulation in mussel digestive gland, and lysosomal membrane stability (LMS), and using additional information provided by body burden analysis to compare the sensitivity of these parameters. The histochemical measurements for both LF and LMS gave a clear indication of a high level of stress in animals from the PAH contaminated site. This LF accumulation in lysosomes is the result of peroxidation of autophagocytosed proteins associated with protein aggregates and oxidatively damaged organelles. These measurements were able to detect the effects of PAHs, and showed a strong relationship with the body burden results.     

Effects of the 'Prestige' oil spill on macroalgal assemblages: large-scale comparison

An assessment of the effects of the 'Prestige' oil spill on intertidal, macroalgal assemblages was carried out comparing abundance data obtained before and after the spill. Four zones in the North and Northwest coast of Spain were sampled, one of them located at the immediate vicinity of the spill, the zone most heavily oiled. Macroalgal assemblages had similar structure between years. Neither critical decrease in abundance of the dominant macroalgae, nor increase in opportunistic species were found. Some differences in abundance were observed, but they did not show any pattern, being more likely the result of the natural variability of the assemblage. Extensive, but not intense fuel deposition on the shores and a limited use of aggressive cleanup methods are suggested as possible causes for the lack of the effects in these assemblages after the 'Prestige' oil spill.     

Modelling distribution of marine benthos from hydroacoustics and underwater video

Broad-scale mapping of marine benthos is required for marine resource management and conservation. This study combines textural derivatives based on bathymetry from multibeam hydroacoustics with underwater video observations to model and map sessile biota between 10- and 60-m water depth over 35 km² in Point Addis Marine National Park (MNP), Vic., Australia. Classification tree models and maps were developed for macroalgae (all types, mixed red algae, Ecklonia, and rhodoliths) and sessile invertebrates (all types, sponges, and ascidians). Model accuracy was tested on 25% of the video observation dataset reserved from modelling. Models fit well for most macroalgae categories (correct classification rates of 67–84%), but are not as good for sessile invertebrate classes (correct classification rates of 57–62%). The poor fit of the sessile invertebrate models may be the combined result of grouping organisms with different environmental requirements and the effect of false absences recorded during video interpretation due to poor image quality. Probability maps, binary single-class maps, and multi-class maps supply spatially explicit, detailed information on the distribution of sessile benthic biota within the MNP and provide information at a landscape-scale for ecological investigations and marine management.     

Permeameter data verify new turbulence process for MODFLOW

A sample of Key Largo Limestone from southern Florida exhibited turbulent flow behavior along three orthogonal axes as reported in recently published permeameter experiments. The limestone sample was a cube measuring 0.2 m on edge. The published nonlinear relation between hydraulic gradient and discharge was simulated using the turbulent flow approximation applied in the Conduit Flow Process (CFP) for MODFLOW-2005 mode 2, CFPM2. The good agreement between the experimental data and the simulated results verifies the utility of the approach used to simulate the effects of turbulent flow on head distributions and flux in the CFPM2 module of MODFLOW-2005.     

A scaled experiment for the verification of the SeaBed Logging method

SeaBed Logging (SBL) is an application of the marine controlled source electromagnetic (CSEM) method that is used to directly detect and characterize possible hydrocarbon-bearing prospects. Although the CSEM method has been used by academia for more than three decades, the application as a direct hydrocarbon indicator was first introduced about five years ago. The central idea of SBL is the guiding of electromagnetic energy in thin resistive layers within conductive sediments. Even if it has been well known for a long time that electromagnetic signals can propagate from a conductive region to another via resistive regions such as air or resistive parts of the lithosphere, the application to hydrocarbon exploration has not been developed until recently. This might be due to the uncertainty of getting any significant response from thin resistive layers such as hydrocarbon reservoirs since electromagnetic energy is highly attenuated in conductive sediments. Thus, during the early development phase of the SBL technique, a scaled laboratory experiment was performed to validate if a thin resistive layer (e.g. hydrocarbons) buried within conductive media (e.g. sediments) could be remotely detected by using electric dipoles as sources and receivers. Data from this experiment were compared to a forward modelling code for layered media, and the comparison showed good agreement between experimental and theoretical results. This suggested that thin resistive layers buried in conductive media are detectable due to the guiding of the electromagnetic field within the resistor. The successful results were vital for realizing the application of marine CSEM as a hydrocarbon exploration technique. We here present the results of the first scaled SBL experiment.     

Histopathology alterations and histochemistry measurements in mussel, Mytilus edulis collected offshore from an aluminium smelter industry (Norway)

Histopathological characteristics of specific organs express condition, and represent time-integrated impacts on the organism stemming from alterations at lower levels of biological organisation. As integrative parameters, histochemical investigations have proved to be sensitive tools to detect effects of chemical compounds.The objective of this study was to determine changes in the tissues of mussels collected at a PAH contaminated site compared to a reference site using histopathological and histochemical parameters: lipofuscin (LF) accumulation in mussel digestive gland, and lysosomal membrane stability (LMS), and using additional information provided by body burden analysis to compare the sensitivity of these parameters.The histochemical measurements for both LF and LMS gave a clear indication of a high level of stress in animals from the PAH contaminated site. This LF accumulation in lysosomes is the result of peroxidation of autophagocytosed proteins associated with protein aggregates and oxidatively damaged organelles. These measurements were able to detect the effects of PAHs, and showed a strong relationship with the body burden results.     

梁端楔形翼缘连接钢框架抗震性能和抗震设计研究

根据两跨两层梁端楔形翼缘连接钢框架试件的低周反复加载试验,研究了梁端楔形翼缘连接钢框架结构在地震作用下的滞回性能、耗能机制、耗能能力和破坏形态。结果显示,试件破坏模式为延性,塑性铰出现在梁翼缘变化处,远离梁柱交界,梁端楔形翼缘连接钢框架具有良好的抗震性能。针对梁端楔形翼缘连接钢框架的特点,提出了强柱弱梁、强节点弱杆件和节点域抗震设计要求。     

Deep-seated faults and lineaments, and the location of earthquake epicenters in the Russian northeast on land

This paper summarizes the available geological and geophysical material for faults as regards their role in the seismic process. The entirety of the geological and geophysical evidence is used to reveal hidden faults, which are important in influencing the spatial distribution of earthquakes, and to produce a map of the major earthquake-generating faults and lineaments in the Russian northeast. As well as the occurrence of earthquakes at known faults that have surface expression, we find that seismicity tends to occur at the hidden faults and lineaments we have identified, as well as at intersections of faults. We made a quantitative assessment of the relationship of seismicity to tectonic fragmentation of the crust, correlating the density and discordance measure for faults to indicators of seismic activity (rate and energy release of earthquakes per unit area) for the southeast flank of the Okhotsk-Lena seismic region. The results obtained in this study revealed some features in the spatial distribution of earthquakes occurring on land in the Okhotsk-Lena seismic region: the maximum level of seismic activity occurs in areas with moderate values of the discordance measure for faults (12 < ‖D‖ ≤ 18) as identified from gravity data and in zones of increased horizontal gradients of the lines of equal discordance. At these locations, the greatest probability of earthquake occurrence for events of energy class K ≥ 12 corresponds to moderate values of the density of faults visible at the surface (0.12 < τ ≤ 0.16 km^?1).     

Original technologies for proven performances for the new LCPC earthquake simulator

The European Quaker project has been a powerful opportunity to accelerate the development of the ability to carry out earthquake simulations at reduced scale in the centrifuge in LCPC—France. This paper summarizes the main original technologies of this simulator. The quality of the checked performances is demonstrated in terms of ability to perform since earthquakes as well as to simulate scaled records of real earthquakes. The consistancy of the results is presented in the time and in the frequency domains.