CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.     

Density perturbations in a Universe consisting of fluid plus collisionless neutrino gas

In a two-component Universe which consists of fluid (visible matter) plus collisinless massive neutrino gas (dark matter), the remarkable difference between the developed inhomogeneities in two components could be formed after the decoupling time. Whether the initial perturbation was in which of the two components, the inhomogeneities developed in visible matter are larger than that in neutrinos, especially on smaller scales. The necessary condition for such a situation to arise is only that the density of neutrinos in the Universe is dominant. That means the non-dominant visible component in the Universe is strongly clustered especially on smaller scales, while the distribution of the dominant dark matter (neutrinos) is fairly uniform.     

The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallop Chlamys farreri

1INTRODUCTION Since the first use in human genetic research in1966,allozyme has become the most popular genetic marker for population genetics studies for almost all groups of animals and plants(Lewontin and Hubby,1966;Murphy et al.,1996).In the past seve…     

鱼卡煤田大煤沟组高分辨率层序地层特征及聚煤规律

利用高分辨率层序地层学理论和技术方法,综合地震、测井、岩芯和煤质资料对柴达木盆地北缘鱼卡煤田中下侏罗统大煤沟组地层河湖三角洲沉积体系进行高分辨率层序地层学分析,从中识别出短期、中期、长期3个级别的基准面旋回层序,划分出向上"变深"非对称型、向上"变浅"非对称型及对称型3种短期基准面旋回基本结构类型。依据等时地层对比法则进行不同级次基准面旋回层序的联井对比,建立了大煤沟组的等时地层格架,在等时地层格架内进行了煤层对比,并在等时地层格架内分析了聚煤规律。     

高能偏振能量色散X射线荧光光谱仪应用现状和进展

对高能偏振能量色散X射线荧光光谱仪基本特点及其用于环境、生物、地质样品中痕量元素的分析和RoSH检测等领域的应用现状予以介绍,并对二次靶与滤光片在痕量元素分析中的应用作了探讨。     

土壤中氟的形态分析

以宁夏盐池地区高氟土壤为例,采用连续提取法对土壤样品中各形态氟进行提取,离子色谱法测定各形态氟的含量。根据研究目的及土壤特点将氟的形态划分为水溶态、离子交换态、可还原态、可氧化态及残渣态5种形态;对各种形态连续提取过程中使用的提取液进行了选择。采用建立的方法获得提取土壤中F-的检出限为0.76μg/g;方法精密度(RSD,n=7)各形态氟为水溶态氟11.3%,离子交换态氟13.5%,可还原态氟10.7%,可氧化态氟8.9%。     

南海西部海域新生代构造古地理演化及其对油气勘探的意义

南海西部海域构造复杂,主要发育有北东—北东东向、北西向和近南北向3组深大断裂。其中,北西向断裂与板块汇聚、碰撞有关,多具走滑性质;北东—北东东向断裂具有与中国东部裂谷盆地相似的发育特点,呈张扭性质;近南北向断裂可能是南海在扩张活动期间于洋、陆壳过渡部位形成的走滑调节断裂,是洋盆扩张的西部边界。新生代里,南海经历了4次成盆事件与3期扩张活动,盆地经历了古新世—中始新世陆缘断陷、渐新世—早中新世扩展与中中新世以来的热沉降3个演化阶段。陆缘断陷阶段的充填系列主要是北东—北东东向与北西向的河流—冲积扇、湖泊沼泽等陆相沉积及火山岩等;盆地扩展阶段表现为中-小型断陷、断-坳陷逐渐复合与联合为大-中型坳陷,古地理格局逐渐由河流与湖沼陆相环境演变为滨海至浅海相的沉积环境;热沉降阶段的成盆活动逐渐减弱以至停止,地层表现超覆,盆地出现联合迹象。结合以往勘探与油气资源调查成果分析,认为南海西部海域陆架陆坡区发育的大-中型沉积盆地石油地质条件良好,蕴藏着丰富的油气资源,勘探潜力巨大。     

寒区线性工程沿线冻土区的植被恢复

寒区油气管道、公路、铁路等线性工程占地、建设和开挖对沿线寒区生态环境是一个切割、破碎的过程, 对自然植被和下伏冻土造成了很大的扰动. 管道泄露引发的油污还可引起植被的退化和死亡. 植被覆盖层破坏后改变了原有的地气界面之间的水、热交换条件和力学性质, 反过来又可加速引发下伏冻土和线性工程地基的退化. 基于保护线性工程地基及下伏冻土的目的, 同时顺应环境保护的要求, 目前就寒区线性工程的植被恢复问题已经有许多探索和实践. 当前, 寒区植被恢复注重最低限度的人为介入干预下的自然恢复, 根据线性工程沿线土壤、湿度、营养条件、物种分布和丰度, 视具体情况选择物种, 确定建植方法. 阿拉斯加管道和青藏铁路植被恢复上的经验和方法, 可为拟建的冻土区中俄输油管道项目沿线的植被恢复问题提供科学的参考和借鉴.     

中俄原油管道沿线典型土样冻胀性试验研究

中俄输油管道穿越约500 km的多年冻土区,管道沿途地形起伏,水系和沼泽发育,使得管道工程地质条件复杂.沿线地貌单元主要可分为沼泽湿地、阶地、河漫滩、坡地以及基岩山脊等5种,不同地貌单元的岩性和冻土赋存条件均不相同.选取各地貌单元的典型土样进行颗粒分析、冻胀率等试验,分析了土样冻胀率与天然含水率、干容重、粘粒含量之间的关系.结果表明:全-强风化花岗岩冻胀率最小,冻土工程地质条件最好;含有机质粉质粘土冻胀率最大,管基需要进行特殊处理;粘土和粉土冻胀率介于二者之间,对管基进行常规处理即可.该试验定量评价了中俄输油管道沿线不同地貌单元的冻胀性,为管道在确保工程长期稳定性的基础上,选择经济、环保的施工方式提供了试验依据.     

中国-俄罗斯原油管道工程(漠河-大庆段)冻土工程地质考察与研究进展

在2005-2007年期间,先后3次对中国-俄罗斯原油管道漠河-大庆段沿线的冻土工程地质条件等进行科学考察,开展了冻土工程地质条件及其在气候变化和人类活动作用下的评价和预测研究.考察研究结果表明:管道沿线多年冻土在各类融区、季节冻土和水系等分隔作用下呈片状或岛状分布,沿线岛状、稀疏岛状及零星岛状占多年冻土区段的40%左右;管道沿线多年冻土随着气候的转暖和人类活动的影响不断退化.地形地貌单元、植被分布、地表水分条件的变化等局部因素对多年冻土的分布和地下冰的赋存产生重要的影响,管道沿线大约分布有50 km左右的沼泽湿地,其表层为腐殖质土及泥炭层,泥炭层下面分布着含土冰层或地下冰,是管道沿线最差的冻土工程地质地段;由于中俄原油管道沿线水系发育多,冻胀丘、冰椎和冰幔等不良冻土现象广泛分布.科学考察的成果为管道沿线冻土工程地质条件评价和预测、管道的稳定性影响分析以及后期的长期检测系统设置等研究奠定坚实的基础,进一步为即将开工的中俄原油管道漠河-大庆段工程的设计、施工提供科学依据.