Toxicity of ammonia to surf clam (Spisula solidissima) larvae in saltwater and sediment elutriates

Ammonia is a natural component of sediments and has been identified as a common contributor to toxicity in marine sediment, elutriate and porewater testing. In our study, the role of ammonia as a possible toxicant in sediment toxicity tests was evaluated using larvae of the surf clam, Spisula solidissima. Elutriates were prepared and tested using six baseline sediment samples. Ammonia was then purged from aliquots of the baseline sediment samples prior to elutriate preparation. Finally, ammonia was spiked into aliquots of the purged elutriates to mimic ammonia concentrations measured in the baseline elutriates. Toxicity was present in all of the baseline samples and was removed in the ammonia purged samples. In most cases, toxicity was comparable in the ammonia spiked samples to levels measured in the baseline samples. Water only toxicity tests revealed that larvae of the surf clam are one of the more ammonia sensitive marine species. The LC50 for survival was 10.6?mg/L total ammonia (.53?mg/L unionized ammonia) and the EC50 for shell development was 2.35?mg/L total ammonia (.12?mg/L unionized ammonia). Toxicity endpoints calculated from the water only ammonia toxicity test were good predictors of effects observed in the sediment elutriate tests.     

Microbial Diversity of the Hypersaline Sidi Ameur and Himalatt Salt Lakes of the Algerian Sahara

Microbial populations within hypersaline lakes often exhibit high activities of photosynthesis, dissimilatory sulphate reduction and other processes and, thus, can have profound impacts on biogeochemical cycles of carbon, nitrogen, sulphur and other important elements within arid lands. To further understand these types of ecosystems, the physicochemical and biological properties of Sidi Ameur and Himalatt Salt Lakes in the Algerian Sahara were examined and compared. Both lakes were relatively neutral in pH (7.2-7.4) and high in salt, at 12% and 20% (w/v) salinity for Himalatt and Sidi Ameur Lakes, respectively, with dominant ions of sodium and chloride. The community compositions of microbes from all three domains (Bacteria, Archaea and Eukarya) were surveyed through the use of 16S and 18S ribosomal gene amplification and clone library clustering using amplified ribosomal DNA restriction analysis (ARDRA) in conjunction with DNA sequencing and analysis. A high level of microbial diversity, particularly among the bacteria of the Himalatt Salt Lake and archaea of Sidi Ameur Lake, was found within these environments. Representatives from all known halophilic bacterial phyla as well as 6 different genera of halophilic archaea were identified. Moreover, several apparently novel phylotypes among both archaea and bacteria were revealed.     

Variations of phytoplankton community structure related to water quality trends in a tropical karstic coastal zone

Phytoplankton community structure in coastal areas is a result of various environmental factors such as nutrients, light, grazing, temperature, and salinity. The Yucatan Peninsula is a karstic tropical region that is strongly influenced by submerged groundwater discharge (SGD) into the coastal zone. Phytoplankton community structure and its relationship with regional and local water quality variables were studied in four ports of the northwestern Yucatan Peninsula. Water quality was strongly related to SGD, and variations in phytoplankton community structure were related to local nutrient loading and hydrographic conditions, turbulence, and human impacts. Our study provides an ecological baseline for the Yucatan Peninsula and serves as a basis for establishing monitoring programs to predict changes at sites with high hydrological variation and in developing an early alert system for harmful toxic algal blooms.     

Drifting blooms of the endemic filamentous brown alga Hincksia sordida at Noosa on the subtropical east Australian coast

Since 2002, the usually uncommon endemic filamentous brown alga Hincksia sordida (Harvey) Silva (Ectocarpales, Phaeophyta) has formed nuisance blooms annually during spring/early summer at Main Beach, Noosa on the subtropical east Australian coast. The Hincksia bloom coincides with the normally intensive recreational use of the popular bathing beach by the local population and tourists. The alga forms dense accumulations in the surf zone at Main Beach, giving the seawater a distinct brown coloration and deterring swimmers from entering the water. Decomposing algae stranded by receding tides emit a nauseating sulphurous stench which hangs over the beach. The stranded algal biomass is removed from the beach by bulldozers. During blooms, the usually crowded Main Beach is deserted, bathers preferring to use the many unaffected beaches on the Sunshine Coast to the south of Main Beach. The bloom worsens with north-easterly winds and is cleared from Noosa by south easterly winds, observations which have prompted the untenable proposal by local authorities that the bloom is forming offshore of Fraser Island in the South Pacific Ocean. The Noosa River estuarine system/Laguna Bay is the more probable source of the bloom and the nutrient inputs into this system must be substantial to generate the high bloom biomass. Current mitigation procedures of removing the blooming alga off the beach with bulldozers treat the symptom, not the cause and are proving ineffective. Environmental management must be based on science and the Noosa bloom would benefit greatly from the accurate ecological data on which to base management options.     

Changes in body condition and fatty acid composition of wild Mediterranean horse mackerel (Trachurus mediterraneus, Steindachner, 1868) associated to sea cage fish farms

Net-cage fish farms attract a great number of wild fishes, altering their behaviour and possibly their physiology. Wild Mediterranean horse mackerel (Trachurus mediterraneus), sampled from populations aggregated around two Mediterranean fish farms and from two natural control populations, were analyzed for differences in body condition, stomach content and fatty acid composition. Pellets used to feed caged fish in both farms were also analyzed to identify their relationship with the fatty acid composition of tissue of wild fish. T. mediterraneus aggregated around the farms throughout the year although large seasonal changes in abundance and biomass occurred. Wild fish aggregated at farms mainly ate food pellets while control fish fed principally on juvenile fish and cephalopods. Wild fish that fed around the cages had a significantly higher body fat content than the control fish (7.30+/-1.8% and 2.36+/-0.7%, respectively). The fatty acid composition also differed between farm-associated and control fish, principally because of the significantly increased levels of linoleic (C18:2omega6) and oleic (C18:1omega9) acids and decreased docosahexaenoic acid (C22:6omega3) in farm-associated fish. The increased condition of wild fish associated with farms could increase the spawning ability of coastal fish populations, if wild fish are protected from fishing while they are present at farms. The fatty acids compositions could also serve as biomarkers to infer the influence of a fish farm on the local fish community, helping to better describe the environmental impact of fish farming.     

Estimating ground water discharge by hydrograph separation

Iron Mountain is located in the West Shasta Mining District in California. An investigation of the generation of acid rock drainage and metals loading to Boulder Creek at Iron Mountain was conducted. As part of that investigation, a hydrograph separation technique was used to determine the contribution of ground water to total flow in Boulder Creek. During high-flow storm events in the winter months, peak flow in Boulder Creek can exceed 22.7 m3/sec, and comprises surface runoff, interflow, and ground water discharge. A hydrograph separation technique was used to estimate ground water discharge into Boulder Creek during high-flow conditions. Total ground water discharge to the creek approaches 0.31 m3/sec during the high-flow season. The hydrograph separation technique combined with an extensive field data set provided reasonable estimates of ground water discharge. These estimates are useful for other investigations, such as determining a corresponding metals load from the metal-rich ground water found at Iron Mountain and thus contributing to remedial alternatives.     

Coastal seas as a context for science teaching: a lesson from Chesapeake Bay

Lessons that employ authentic environmental data can enhance the ability of students to understand fundamental science concepts. This differs from traditional "environmental education" in that school curricula need not set aside time for educators to teach only environmental topics. Rather, the "environment" is used to advance student learning in science and technology. The success of this approach depends on programs that encourage scientists to communicate more effectively with teachers at all education levels. The expanding diversity of research and monitoring activities on the world's marine waters constitutes an outstanding potential education resource. Many of these projects involve remote sensing with sophisticated instrumentation and employ Internet technology to compile measurements, interpret data using graphs and satellite imagery, and share the results among scientific colleagues and the general public alike. Unfortunately, these resources, which constitute a much shortened path between research findings and textbook presentation, are seldom interpreted for use by K-12 educators. We have developed an example that uses the Chesapeake Bay as a paradigm to demonstrate how such interpretation can assist educators in teaching important principles in physical oceanography and marine ecology. We present this example using PowerPoint to conduct a virtual tour of selected Internet sources. Our example begins with the conceptual "salt wedge" circulation model of Chesapeake Bay as a partially mixed estuary. Teachers have the opportunity to explore this model using salinity, temperature, and dissolved oxygen data taken from a research vessel platform during summer professional development programs. This source of authentic data, originally obtained by teachers themselves, clearly demonstrates the presence of a picnocline and deep-water anoxia. Our lesson plan proceeds to interpret these data using additional Internet-based resources at increasing scales of time and space. The "salt wedge," picnocline, and anoxia are examined using graphics derived from data taken by researchers using "ScanFish," a towed instrument that samples temperature, salinity, and dissolved oxygen at a resolution of only a few meters vertically and horizontally. The seasonal dynamics of these parameters at a given location are interpreted using biweekly monitoring data obtained as part of the state-federal Chesapeake Bay Program. The influence of annual variations in freshwater input is examined using stream flow data from US. Geological Survey gauging stations. Satellite remote sensing images from the TOPEX/Poseidon project are used to show how El Ni?o and La Ni?a events in the mid-Pacific affect the Chesapeake Bay system via rainfall on its watershed. Finally, the life cycle of the blue crab (Callinectes sapidus) is presented to show how an estuarine organism has adapted to this truly unique and dynamic coastal environment.