Estrogenic responses to xenobiotics in channel catfish (Ictalwus punctatus)

Several environmentally relevant contaminants are characterized as xenoestrogens by virtue of their ability to induce responses similar to 17β-estradiol (E2). There is concern that exposure to these xenoestrogens may result in endocrine and, thus, reproductive disruption. The objective of the present research was to determine whether xenobiotics known to elicit estrogenic responses in mammals were estrogenic in channel catfish, and if these compounds were capable of altering normal physiological response to E2. Sexually immature catfish were exposed by intraperitoneal injection to E2, suspected xenoestrogens or combination doses of E2 and xenoestrogen. Appearance of vitellogenin (Vg) in serum was used as the bioindicator of estrogenicity; Vg was measured by ELISA 7 days after injection. The ED₅₀ of E2 for the appearance of Vg in blood, 0.6 mg/kg, was used as the positive control. Synthetic estrogens, ethinylestradiol, mestranol and DES were effective in inducing Vg; the antiestrogen tamoxifen inhibited response to E2 when given in a combination dose. Treatment by both methoxychlor and p-nonylphenol resulted in Vg appearance in serum; the doses required were 300 times that of E2, and the vitellogenic response was less when compared to the E2-induced response. In addition, a combination dose of methoxychlor with E2 decreased the magnitude of the response to E2. Other mammalian xenoestrogens, o,p′-DDT, chlordecone, lindane and β-HCH, were not able to produce a vitellogenic response in this study.     

Video observations of marine gravel transport

In an analysis of video observations of gravel transport in the West Slent, UK, collective and individual characteristics of 458 bursts of bedload have been examined and the transport velocity and distance of 1680 individual particles in a consecutive succession of burst events determined. Infrequent, long duration, high stress events account for 60% of total transport in only 24% of the total time with the mean free path and transport velocity for particles being 0.16 m and 0.22 m/s, respectively. Clear links between intense intermittent turbulence and bedload transport revealed in this study, suggest progress in modelling requires a clearer understanding of near bed flow structure.     

Modern swathe sounding and sub-bottom profiling technology for research applications: The Atlas Hydrosweep and Parasound Systems

This chapter describes two separate but complementary research echosounder systems originally developed by Krupp Atlas Elektronik GmbH for the new German oceanographic research vessel Meteor.The Hydrosweep is a Hydrographic wide-swathe sweep survey echosounder for both shallow and deep water applications providing accurate bathymetric surveys and terrain-following navigation capabilities.The Parasound system is a hull-mounted dual channel parametric narrow-beam deep sea survey and sub-bottom profiling echosounder enabling particularly high vertical and horizontal resolution of seabed features.     

Three-dimensional visualization of mission planning and control forthe NPS autonomous underwater vehicle

The Naval Postgraduate School (NPS) is constructing a small autonomous underwater vehicle (AUV) with an onboard mission control computer. The mission controller software for this vehicle is a knowledge-based artificial intelligence (AI) system requiring thorough analysis and testing before the AUV is operational. The manner in which rapid prototyping of this software has been demonstrated by developing a controller code on a LISP machine and using an Ethernet link with a graphics workstation to simulate the controller's environment is discussed. The development of a testing simulator using a knowledge engineering environment (KEE) expert system shell that examines AUV controller subsystems and vehicle models before integrating them with the full AUV for its test environment missions is discussed. This AUV simulator utilizes an interactive mission planning control console and is fully autonomous once initial parameters are selected     

Numerical simulation of unsteady free-surface motions using a boundary integral formulation

A numerical time simulation method is described to solve fluid flow problems including unsteady free surface motion. The method is based on potential flow theory. At every time step, the problem is solved using a boundary integral formulation of the fluid domain. The linearized free surface conditions are integrated in time and the solution is marched forward. Computational results simulating the free surface motion for the cases of a linear progressive wave, wave propagating into a region of calm water and the wave maker motion are presented. Comparison with theoretical results demonstrate the feasibility of the proposed simulation scheme.     

Properties of structure and function of cadmium-binding proteins/peptides from Euglena gracilis

Euglena gracilis makes two small Cd-binding proteins/peptides (Cd-BP I and Cd-BP II) in response to exposure to Cd²⁺. These proteins migrate slower than mammalian Cd-metallothionein (Cd-Mt) through Sephadex G-75, but like Cd-Mt also contain Zn and are separated into two species when chromatographed over a DEAE column. Both BPs are much less stable than Cd-Mt under acidic conditions. One source of this instability is that at least Cd-BP II contains acid-labile sulfide co-ordinated to the metals. It has been quantitated in Cd-BP II and shown to have a value of 1·25 ± 0·10 S²⁻/Cd. The reactivity of the Cd-binding sites in ligand substitution and thioldisulfide exchange reactions has been assessed. The reactions of Cd-BP I and II with pyridylazoresorcinol (PAR) were slow and displayed complex kinetic behavior. Similarly, both Cd-BPs react slowly and with complicated kinetics with 5,5′-dithiobis-(2-nitrobenzoate).     

Trace metal concentrations in sediments of the Eastern Mississippi Bight

Sediments collected from 3 onshore-offshore transects (12 stations total) in the Eastern Mississippi Bight at 5 different times during 1987–1988 have been analyzed for total Ag, As, Ba, Cd, Co, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Sb, Se, Sn, Th and Zn. The data show considerable variation both spatially and temporally, largely as a result of natural variability in grain size and mineralogy. Clay-rich samples from deep water were always more metal-rich than sandy samples from shallow areas. Many samples from near the Mississippi River Delta appeared to be enriched in Ba by about a factor of two, probably as a result of contamination from oil well drilling mud, but there were few other indications of pollution influences. Many samples from throughout the area gave Mn/Fe and Cd/Fe ratios a factor of two or more lower than their Mississippi River source material. This shows that biochemical activity in the sediment is capable of solubilizing reducible and adsorbed metals.     

The depletion of oil

The trend of previous estimates of the world's ultimate recovery of oil suggests a figure of 1650 BBO, of which 1438 BBO have been discovered and 978 BBO remain to be produced. Some large reported reserve figures are questioned. Importance is attached to the mid-point of depletion when rising production tends to give way to decline. The share of the world's supply coming from six Middle East countries is set to continue to rise from its 1986 low of 16% to as much as 50% by 2010, with the likely consequence of higher oil prices and serious political implications. The world is rapidly approaching a turning point when it has to face the consequences of an irreversible decline in oil production.     

The distributions of particulate atmospheric trace metals and mineral aerosols over the Indian Ocean

Data are presented for the concentrations of Al, Fe, Mn, Ni, Co, Cr, V, Cu, Zn, Pb and Cd in aerosols collected over two contrasting regions of the Indian Ocean. These are: (1) the northern Arabian Sea (AS), from which samples were collected in the northeast monsoon, during which the region receives an input of crustal material from the surrounding arid land masses; and (2) the Tropical Southern Indian Ocean (TSIO), a remote region from which samples were collected from air masses for which there were no large-scale up-wind continental aerosol sources. The TSIO samples can be divided into two populations: Population I aerosols, collected from air masses which have probably impinged on Madagascar, and Population II aerosols, which have been confined to open-ocean regions to the south of the area.The data indicate that there are strong latitudinal variations in the chemical signatures of aerosols over the Indian Ocean. The input of crustal material to the Arabian Sea gives rise to an average Al concentration of about 1000 ng m⁻³ of air in the northeast monsoon regime. As a result, the concentrations of all trace metals are relatively high, and the values of crustal enrichment factors are less than 10 for most metals, in the AS aerosols. In contrast, TSIO Population II ‘open-ocean southern air’ sampled during the southwest monsoon season, has an average Al concentration of only about 10 ng m⁻³ of air. Trace metal concentrations in the TSIO ‘open-ocean southern air’ during the southwest monsoon season are representative of ‘clean’ remote marine air and are generally similar to those reported over the central North Pacific.Mineral dust concentrations over the Indian Ocean decrease in a north to south direction, from about 15–20 μg m⁻³ of air in the extreme north to about 0.01–0.25 μg m⁻³ of air in the far south. The deposition of mineral dust over the northern Arabian Sea can account for approximately 75% of the non-carbonate material incorporated into the underlying sediments.In the Arabian Sea the dissolved atmospheric inputs of all the trace metals, with the exception of Cu and Co, exceed those from fluvial run-off by factors which range from 9.6 for Pb to 1.6 for Cr.