Protective effects of Ecklonia cava extract on the toxicity and oxidative stress induced by hair dye in in-vitro and in-vivo models

Oxidative hair dyes containingρ-phenylenediamine(PPD)are reported to induce an allergic reaction by promoting oxidative stress when absorbed through the skin.Despite the associated risk,these hair dyes remain popular owing to their convenience and sharpness of color.This makes it important to minimize the cytotoxicity and oxidative stress induced by PPD-containing hair dyes.Ecklonia cava extract has been evaluated in different studies for its protective effects against external stress in fibroblasts and keratinocytes.Our study was aimed at using in-vitro and in-vivo models to investigate the extract’s effects on cytotoxicity of and oxidative stress induced by PPD-containing hair dyes.Analysis of CIEL*a*b*Color space was first used to determine the range of E.cava extract that would not interfere with the coloring ability of the dye upon addition.Subsequently,the set ranges of E.cava extract(5% and 7%)were added to the hair dye and their toxicity assessed by evaluating the viability of fibroblasts and keratinocytes.The effects on developmental phenotypes and induction of oxidative stress by hair dye were evaluated and compared with those of hair dyes containing different contents of E.cava extract using an in-vivo zebrafish model.Our results showed that E.cava extract in hair dye could significantly decrease the cytotoxicity and levels of oxidative stress caused by hair dyes containing PPD in both in-vitro and in-vivo models.These results suggest that the addition of 7% E.cava extract to 250μg/mL hair dye does not interfere with the coloring ability of the dye while showing significant protective eff ects against the hair dye.The study proposes that the use of E.cava extract as an adduct to hair dyes containing PPD reduces the cytotoxicity and oxidative stress induced by these hair dyes.     

急性UVB辐射对粉叶马尾藻过氧化损伤和主要抗氧化酶的影响

粉叶马尾藻(Sargassum glaucescens)是台湾地区潮间带的重要大型藻类,其对紫外线增强的生理响应特征尚不清楚。本研究关注粉叶马尾藻在急性紫外线辐射(Ultraviolet radiation B,UVB)下的生理指标变化,探索了粉叶马尾藻在急性UVB辐照下抗氧化系统的瞬时反应过程。结果表明:低强度组[0.5W/(m2·s)]藻体在24h内未出现显著氧化损伤和蛋白质含量变化,但是细胞活力在辐照12h后显著降低,藻体超氧化物歧化酶(SOD)酶活性随辐照时间延长而降低,过氧化氢酶(CAT)酶活性呈现升高后下降的趋势,抗坏血酸过氧化物酶(APX)酶活性没有出现显著降低;高强度组[2W/(m2·s)]藻体在辐照3h后藻体出现明显氧化损伤,6h后细胞活力和蛋白质含量显著降低,藻体SOD酶活性随辐照时间延长而降低,CAT和APX酶活性呈现升高后降低的趋势,辐射12h后即显著降低。低强度UVB辐射对粉叶马尾藻的影响相对较小,表明粉叶马尾藻对UVB辐射具有一定耐受性;而高强度UVB辐射的影响较为显著,意味着UVB辐射的持续增强将超出粉叶马尾藻的耐受限度,进而造成不利影响。本研究结果初步揭示了粉叶马尾藻对于急性UVB辐射的增强的生理响应特征,为进一步研究UVB辐射增强的生态学效应提供参考依据。     

Identifying Indicator Species in Habitats Created by Coastal Structures

In this paper,three approachs were developed to find indicator species from the habitat created by coastal structures.These approachs consist of a model of species co-occurrence probability,a model of k-environmental factor probability and a composite model.Simultaneously,a case study was conducted in Hsinchu Fishing Port of north-western Taiwan.Based on the aforementioned models,three primary producer species,Ahnfeltiopsis flabelliformis,Chondrus ocellatus and Sarcodia montagneana,were chosen as the indicator species which had the highest co-occurrence probabilities and showed greater tolerance to more critical environment.It is imperative to understand how the three species under particular co-occurring conditions and environmental factors influence the composition of sessile assemblages in coastal water.The results indicate that for the purpose of increasing biodiversity,these models are feasible to find indicator species of artificial structures,and to help make it possible to design coastal structures based on biological considerations.This study provides an innovative approach for further advanced application in the artificial habitat of coast management.     

西天山两侧前陆盆地晚新生代沉积特征及构造意义

天山两侧前陆盆地晚新生代地层发育,沉积厚度较大。盆地沉积序列的韵律性反映了天山造山带的构造演化历史。晚新生代沉积特征及孢粉组合表明,中新世一上新世一早更新世碎屑岩不稳定组分明显增加,阔叶植物花粉含量减少、耐干地喜早的蒿、藜等显著增加,反映天山活动性增强,上新世晚期天山强烈隆升,天山两侧前陆盆地上新世晚期开始出现磨拉石建造,由于山体隆升造成的大气环流改变,天山两侧盆地封闭性增强,大陆性气候流改变,天     

多导体柱微波成像的连续编码遗传算法

提出一种能同时重建多个二维良导体目标外形轮廓的新方法——连续编码遗传算法．将目标的横截面轮廓近似表达为三角级数形式,由边界条件得到一积分方程组,在此基础上将成像问题转化为约束优化问题,级数的各项系数为待优化量．积分方程组为约束条件,目标函数定义为实际测量的散射场与反演过程中得到的散射场之间的相对误差函数．采用连续编码遗传算法求解,优化过程通过选择、交叉、变异等遗传操作的选代而实现,待优化目标函数进行线性变换并采用模拟退火原理确定目标后表达为适应度函数,采用联赛选择与比例选择相结合的选择机制和单点交叉方式,变异操作通过对基因施加微小随机扰动实现．上一代中适应度最高的个体直接保留．数值模拟反演实验验证了方法的有效性．与其他反演方法相比,本法具有简单、通用、鲁棒性强等特点．     

DIRECT INTERPRETATION OF RESISTIVITY DATA OVER TWO-DIMENSIONAL STRUCTURES*

A direct interpretation scheme is developed which is capable of determining most of the geological features of a ground which can be assumed to be two dimensional in structure. This scheme extends the earlier work of Pekeris (1940) and Koefoed (1968) to the case where the basal layer of a ground is undulating. It also has a limited use for finding the parameters of a dipping dyke in the lower medium. Though the top and dip of the dyke can be determined, this is not true for the thickness.     

台湾崩塌地的分类与防治工法

台湾本岛南北轴长约385km，东西最这处约143km，中央山脉纵贯本岛南北长约350km，其中至少有25个以上的主峰海拔高度超过3000m，台湾山地面积约占总面积70％，不但地质环境复杂，地势陡峻，土壤浅薄、河流短急，降雨多、强度大且集中，山坡地崩塌常随着台风、豪雨及地震、或伴随着人为的破坏还引起，屡屡造成重大灾害。崩塌常发生于特定地质或地质结构上，尤以坡地或台地的缓坡面为最多，其移动缓慢而有特续性及周期（反复）性。本研究针对台湾坡地崩塌的特性试作型态上的分类，并寻求其有效且可行之防治工法，盼能降低灾害所造成之损失，并确保该区域人民之生命及财产之安全。     

THE EFFECT OF SEEING ON SOLAR MAGNETIC FLUX MEASUREMENTS

We investigate the influence of seeing upon measurement of magnetic flux of photospheric fields. For this purpose we quantify seeing variation in one day's observation at Big Bear Solar Observatory in terms of the Fried function, a Modulation Transfer Function for the atmospheric seeing. The temporal variation of seeing quality is compared with that of magnetic flux measured in a quiet region with size 5 \times 4 near the solar disk center. A good correlation is found between the seeing change and apparent evolution of magnetic flux values, implying, as a possibility, that magnetic flux measurement might have been modulated by seeing. Based on a simple model of ensembles of Gaussian magnetic elements we argue that even the net flux as well as the total flux can change due to seeing variation if the magnetograph has a finite detection threshold and if the intrinsic fluxes in one and the other polarities are unbalanced.     

Numerical Study on Plume Interaction Above An Alternating Diffuser in Stagnant Water

1.IntroductionOcean outfall diffusers are used in most sewage disposal schemes to discharge the wastewater asmultiple buoyant jets which can be rapidly mixed with and diluted by the ambient current.Diffuserstake ona varietyof forms,rangingfromone withjets…     

冲绳海槽北部CS1孔岩石磁学特征及其早期成岩作用的影响

以2～4cm为间隔，对冲绳海槽北部的CSH1孔岩心（17．36m）进行了岩石磁学研究．根据岩心上部的两个特征火山灰层和浮游有孔虫组合分析建立了钻孔的时间框架，沉积物底部年龄可能为50ka．除了在三个火山灰层和近表层向下的快速降低外，整个钻孔磁学参数的变化甚小，未能反映出50ka来的气候变化．与大多数沉积物不同，该钻孔大多数层位的S值（S=-IRM-0.3T/SIRM，它代表了低矫顽力磁性矿物的含量）小于0.9，指示钻孔沉积物中存在相当比例的高矫顽力磁性矿物．代表性沉积物样品的磁化率-温度（K-T）曲线除了存在580℃磁铁矿的居里温度之外，还显示200-350℃的居里温度，指示样品中存在铁硫化物（胶黄铁矿-磁黄铁矿）．这与加热过程中有刺鼻的臭味、加热后的沉积物变黑对铁硫化物的指示相吻合．自生成因的铁硫化物的存在以及表层磁性参数的快速降低，都明确表明沉积物遭受了早期成岩作用．除了三个火山灰层以外钻孔的其他层位无特征磁学变化，表明沉积物后来受到的改造作用强烈，磁学参数所蕴含的原始气候信号被破坏．在这一系列氧化-还原过程中消耗了沉积物中的有机质和水溶液中的硫酸盐，造成了沉积物中铁、锰元素的重新溶解和沉淀等．