Bioavailability of heavy metals bound to sediments affected by a mining spill using Solea senegalensis and Scrobicularia plana

After the Aznalcóllar mining spill (April, 1998) different ecosystems along the Guadiamar river and the Guadalquivir estuary were impacted by high concentrations of metals. The concentration of metals Zn, Cd, Pb, Cu and As has been detected as a good tracer of the pathway of the toxic spill. Different individuals of the fish Solea senegalensis and the clam Scrobicularia plana were exposed to contaminated sediments after the accidental spill in the south-west Spain. The induction of metallothioneins in fish and the bioaccumulation of metals in clams were determined in different tissues to determine the biological adverse effect associated with the heavy metals from the spill. A relationship between the enrichment of metals in the tissues and the induction of metallothioneins was determined for those assays performed using contaminated sediments. The bioaccumulation of metals indicates that the risk associated with the spill should be continuously evaluated to establish the sublethal effects related to the accident.     

Caprella watch: a new approach for monitoring butyltin residues in the ocean

A comparative study on the butyltin levels in various organisms showed that marked bioaccumulation occurs in certain lower trophic levels; i.e. from seawater to phytoplankton and into caprellid amphipods, Caprella spp. Caprella spp., which inhabit algal communities and aquaculture beds in the subtidal zone, are small crustaceans with reduced movement and a life-span of less than 3 months. These characteristics indicate that Caprella spp. may be well-suited for monitoring butyltin residue changes over small spatial and temporal scales. Two groups of organisms, mussels and neogastropods, have been mainly used for monitoring butyltin in shallow water ecosystems. These invertebrates mainly inhabit the intertidal zone where the butyltin levels vary widely depending on the immersion period and exposure to the sea surface microlayer. Monitoring using neogastropods may also over estimate exposures after restrictions on tributyltin, since neogastropods show an irreversible response to residue changes owing to their long life-spans. Thus, we propose usage of Caprella spp. to monitor temporal and spatial changes in baseline concentrations of butyltins.     

Using trace elements in skin to discriminate the populations of minke whales in southern hemisphere

Concentrations of 12 trace elements (V, Cr, Mn, Cu, Zn, Se, Rb, Sr, Cd, Cs, Ba, and Hg) were determined in liver and skin tissues of minke whales from various regions within the Antarctic Ocean. Cd concentrations in livers of southern minke whale were apparently higher than those in cetaceans from other regions, while Hg concentrations were lower. There were significant positive correlations between body length and concentrations of Cd and Hg in the liver. The concentrations of all trace elements in the skin were lower than those in other cetaceans reported previously. Significant positive correlations between liver and skin were found for Cr, Mn, Cu, Zn, Rb, Cd, and Cs, implying that the concentrations of these trace elements in the skin reflect those of internal organs. Large interannual variation of the accumulation pattern of trace elements in the skin was observed for the southern minke whales from Area V. There were significant differences in the skin element concentrations among Areas III, IV, and V, especially for males. Also, discriminant analysis between geographically two different groups collected during 1995/1996 austral summer season, based on the concentrations of trace elements in the skin, allowed for a correct classification of 90% of these minke whales. These results suggest that measurement of trace elements in skin samples could provide valuable information on the status of contamination and possible geographic differences in the accumulation levels in southern minke whales.     

The impact of oestrogenic and androgenic contamination on marine organisms in the United Kingdom--summary of the EDMAR programme. Endocrine Disruption in the Marine Environment

This paper summarises results of the EDMAR programme which is investigating oestrogenic and androgenic endocrine disruption in UK coastal waters. Most of the data concern fish. Four species (flounder, viviparous blenny and two sand gobies) are experiencing feminisation in industrialised estuaries. In males this includes vitellogenin (VTG) synthesis, ovotestis induction and/or feminised sexual characteristics. Although reproductive success may be impaired in some cases, implications for fish populations are still unclear. Suspected causative contaminants include natural oestrogenic substances and synthetic oestrogen mimics. The majority of the oestrogenic activity is adsorbed to sediments, and routes of exposure may include benthic food chain transfer. Some natural androgenic substances are also being discharged to estuaries, but their activity appears low.     

海滩地貌及其变化：Ⅱ.海滩地貌的长期变化及海岸建筑物引起的海滩地…

讨论了海滩地貌的长期变化和建筑物对海滩地貌的影响。     

Implications of spatial heterogeneity for management of marine protected areas (MPAs): examples from assemblages of rocky coasts in the northwest Mediterranean

Marine protected areas (MPAs) are increasingly used as a management tool to preserve species and habitats. Testing hypotheses about the effectiveness of MPAs is important for their implementation and to identify informative criteria to support management decisions. This study tested the general proposition that MPAs affected assemblages of algae and invertebrates between 0.0 and 0.5 m above the mean low water level of rocky coasts on two islands in the Tuscan Archipelago (northwest Mediterranean). Protection was concentrated mainly on the west coasts of the islands, raising the possibility that neither the full range of assemblages nor the relevant scales of variation were properly represented within MPAs. This motivated the comparison of assemblages on opposite sides of islands (habitats). The effects of MPAs and habitat were assessed with a multifactorial sampling design; hypotheses were tested about differences in structure of assemblages, in mean abundance of common taxa and in univariate and multivariate measures of spatial variation. The design consisted of three replicate shores for each condition of protected and reference areas on the west side of each island and three unprotected shores on the eastern side. Assemblages were sampled independently four times on each island between June 1999 and January 2001. At each time of sampling two sites were selected randomly at each of two tidal heights to represent midshore and lowshore assemblages on each shore. Estimates of abundance were obtained using non-destructive sampling methods from five replicate 20x20 cm quadrats at each site. Results indicated differences among habitats in structure of assemblages, in mean abundance of common taxa and in univariate and multivariate measures of spatial variation at the scale of shores. Most of these patterns were inconsistent with the predicted effect of management through MPAs. The data suggest that designation of MPAs in the Tuscan Archipelago should proceed through management of multiple shores and types of habitat selected to guarantee protection to a representative sample of assemblages and to the processes responsible for maintenance of spatial patchiness at different scales. This study also shows that considerations of spatial heterogeneity are important to underpin management decisions about the number, size and location of MPAs.     

Sublethal toxicity of orchard stormwater runoff in Sacramento splittail (Pogonichthys macrolepidotus) larvae

The sublethal effects of stormwater runoff from sections of a plum orchard treated with esfenvalerate or diazinon were evaluated in 7-day-old Sacramento splittail (Pogonichthys macrolepidotus) larvae. Fish were exposed to eight runoff samples using the USEPA standard static renewal method for 96 h acute toxicity testing, then transferred to clean water for three-month to assess the survival, growth, histopathological abnormalities, and heat stress proteins (hsp). No significant mortality was observed at 96 h in exposed fish. At one week, histopathological abnormalities included severe glycogen depletion, cytoplasmic protein droplets, vacuolar degeneration, and cell necroses in liver of all exposure groups. Pyknotic nerve cells were seen in brain of one exposure group. Significantly higher cumulative mortality, lower condition factor, and elevated hsp60 and hsp70 levels (p < 0.05) were occurred in several exposure groups. No histopathological abnormalities were observed after three months in any exposure group. This study confirms that standard acute toxicity tests have underestimated the toxicity of stormwater runoff, and although splittail larvae survived the 96 h exposure, they exhibited reduced survival and growth and showed signs of cellular stress even after a three month recovery period.