Dynamic character of the hydrothermal vent habitat and the nature of sulphide chimney fauna

A major substratum for vent organisms on Juan de Fuca and Explorer Ridges of the northeast Pacific is the polymetallic sulphide chimney. The deposition processes and subsequent growth of such chimneys provide a dynamic and extreme habitat that changes rapidly. During initial venting stages, colonization must await stabilization of the chimney structure. As the porous anhydrite shell accumulates sulphide minerals, protection from direct hot water contact increases for organisms colonizing the outer walls while sufficient vent water still diffuses for the support of chemosynthesis. Vestimentiferan worms are found on small sulphide mounds but the active growth and collapse of anhydrite spires is a major source of disruption and mortality. High temperature venting and spire growth appear to attract alvinellid polychaetes which may be implicated in the process of strengthening and sealing the anhydrite spires. Fauna on the growing chimney is subject to changes in fluid flow patterns that can make the structure uninhabitable. Large chimneys are inhabited by more species indicating that a diversification of habitat results from variations in mineralization and fluid availability. Chimney fauna has become specialized in exploiting an unpredictable and unstable habitat in an extreme example of the control of biotic development by the physical environment.     

Effect of liquid storage tanks on the dynamic response of offshore platforms

The sloshing of liquids in storage tanks on fixed offshore structures affects both the natural frequencies and damping. Analytic procedures by which one may account for these effects are presented. Also shown is a method for design of tankage that will result in suppression of dynamic response at the fundamental flexural natural frequency of the structure. An important aspect is that no new equipment is required but only optimum configuration of tankage that is already required for storage of water, fuel, mud or crude oil. Supporting data from full scale field studies are presented.     

Linear and nonlinear interactions of surface waves with bodies by a three-dimensional Rankine panel method

Linear and second-order surface wave interactions with floating and bottom-mounted bodies of realistic geometry are simulated in the time domain by a three-dimensional Rankine panel method. The fundamental stability analysis governing the propagation of transient wave disturbances on a panel mesh distributed on the free surface is carried out from first principles. The radiation condition is enforced by a dissipative beach selected to coincide with an outer annulus of panels. The fundamental physics governing the wave energy absorption is presented and the beach attributes are selected and validated for the linear and second-order problems. Computations are presented of the linear and sum-frequency second-order forces on a single and multiple truncated circular cylinders, and very good agreement is found with benchmark computations. The accuracy and efficiency of this method render it a promising candidate for the study of complex nonlinear wave induced phenomena upon offshore platforms, like springing and ringing.     

Branching of the Tsushima Current in 1981–83

Satellite-derived sea surface temperatures illustrate the variability of the path of the Tsushima Current in the Sea of Japan. In the spring of 1981 the Tsushima Current did not split as it left the Korea Strait and flowed into the Sea of Japan, which is contrary to the historical concept of branching. Warm water remained along Honshu, the main island of Japan, making a strong front oriented in an east-west direction. Hydrographic data confirm that this spring condition lasted through to the fall of 1981. On the other hand, during the springs of 1982 and 1983 the branching is evident from satellite images: one branch flowed northward along the east coast of Korea, and the other flowed eastward along Honshu of Japan.     

Late Quaternary sediments on the outer shelf of the Korea Strait and their paleoceanographic implications

Sedimentological and micropaleontological characteristics of core sediments from the outer shelf of the Korea Strait, which connects the northern East China Sea and the East Sea (Sea of Japan), were investigated to elucidate the paleoceanographic environment, especially the timing of the Kuroshio inflow, since the last glacial maximum. The core sediments, containing continuous records of the last 15,000 years, are characterized by a relatively high mud content (more than 50%, on average) and well-developed tide-influenced sedimentary structures. Their mineralogy suggests that the material originated from the paleo-Nakdong River system, which extended across the shelf of the Korea Strait during low sea-level periods. Planktonic foraminifers reveal a series of well-defined changes in paleoceanographic conditions during the late Pleistocene–Holocene. Down-core variations in the abundance of four foraminiferal assemblages, i.e., cold, coastal, tropical–subtropical, and Kuroshio water groups comprising characteristic planktonic species, suggest the occurrence of a distinct paleoenvironmental change in the surface water at 7,000 years b.p., i.e., from 15,000 to 7,000 years b.p., the area was influenced by coastal waters whereas since ca. 7,000 years b.p., it has been under the influence of open-sea water related to the Kuroshio Current flow, associated with both higher temperature and higher salinity. In particular, Pulleniatina obliquiloculata increased markedly in abundance at this time, documenting the inflow of the Kuroshio into the study area. These data indicate that the coastal water stage terminated at ca. 7,000 years b.p. when the warm Kuroshio and its major branch, the Tsushima Current, began to flow into the East Sea, as is the case today. The intrusion of the Tsushima Current through the Korea Strait after ca. 7,000 years b.p. resulted in abrupt changes in sedimentation rates and a dramatic increase in abundance of the Kuroshio indicator species, P. obliquiloculata.     

Sublethal toxicity of orchard stormwater runoff in Sacramento splittail (Pogonichthys macrolepidotus) larvae

The sublethal effects of stormwater runoff from sections of a plum orchard treated with esfenvalerate or diazinon were evaluated in 7-day-old Sacramento splittail (Pogonichthys macrolepidotus) larvae. Fish were exposed to eight runoff samples using the USEPA standard static renewal method for 96 h acute toxicity testing, then transferred to clean water for three-month to assess the survival, growth, histopathological abnormalities, and heat stress proteins (hsp). No significant mortality was observed at 96 h in exposed fish. At one week, histopathological abnormalities included severe glycogen depletion, cytoplasmic protein droplets, vacuolar degeneration, and cell necroses in liver of all exposure groups. Pyknotic nerve cells were seen in brain of one exposure group. Significantly higher cumulative mortality, lower condition factor, and elevated hsp60 and hsp70 levels (p < 0.05) were occurred in several exposure groups. No histopathological abnormalities were observed after three months in any exposure group. This study confirms that standard acute toxicity tests have underestimated the toxicity of stormwater runoff, and although splittail larvae survived the 96 h exposure, they exhibited reduced survival and growth and showed signs of cellular stress even after a three month recovery period.     

Binding of thorium(IV) to carboxylate, phosphate and sulfate functional groups from marine exopolymeric substances (EPS)

Th(IV) isotopes are important proxies in oceanographic investigations, and are used as tracers of particle dynamics and particulate organic matter (POC) fluxes out of the euphotic zone through the use of ²³⁴Th/POC ratios. These approaches rely on empirically determined and variable POC to ²³⁴Th ratios, which might be controlled, in parts, by the abundance of exopolymeric substances (EPS). EPS contain acidic polysaccharides (APS) and are excreted by both phytoplankton and bacteria. To this end, radiotracer experiments with EPS from microbial cultures were conducted to determine the binding environment of ²³⁴Th(IV)-binding ligands in colloids and suspended particles in marine systems. In these experiments, the ²³⁴Th distribution during isoelectric focusing (IEF) and polyacrylamide gel electrophoresis (PAGE) was related to the functional group composition of EPS and of colloidal organic matter (COM) isolated from the Gulf of Mexico (GOM) using cross-flow ultrafiltration. EPS was extracted from phytoplankton (Emiliania huxleyi and Synechococcus elongatus) and bacteria (Sagittula stellata and Roseobacter gallaeciensis) cultures by repeated alcohol precipitation. Phosphate and sulfate concentrations were determined using ion chromatography (IC). IEF profiles indicated that 49% to 65% of the ²³⁴Th-labeled EPS from plankton and bacteria as well as COM samples from the GOM was found concentrated below pH of 4, near an isoelectric point, pH_IEP, of about 2. The carboxylic acid maxima for extracted EPS and COM samples appeared close to the pH_IEF of ²³⁴Th(IV). The phosphate maximum appeared at the same pH_IEF as ²³⁴Th(IV) for EPS from R. gallaeciensis and S. elongatus. The sulfate maximum was found at the same pH_IEF as ²³⁴Th(IV) for EPS from S. elongatus and COM. The molecular weight (MW) of the strongly Th(IV)-binding ligand varied from 1 to 14 kDa, depending on the species, but was about 10 kDa in COM. Thus, depending on the species of plankton or bacteria, the MW and specific functional group composition of the strongly ²³⁴Th(IV)-binding amphiphilic biomolecule can vary. Therefore, different acidic functional groups can, at times, contribute to the binding of Th(IV) to the EPS chelating ligand, which can also have different MWs. This implies that the binding environment for Th(IV), which is present at total concentrations at least a million times lower than the acid functional groups, consists of strong polydentate chelate complexes in clustered structures of carboxylate, sulfates and/or phosphates. The combination of strongly chelating groups and amphiphilicity gives this biomolecule the unique properties of a “sticky” ligand.     

Stable carbon and nitrogen isotopes of sinking particles in the eastern bransfield strait (Antarctica)

A time-series sediment trap was deployed at 1,034 m water depth in the eastern Bransfield Strait for a complete year from December 25, 1998 to December 24, 1999. About 99% of total mass flux was trapped during an austral summer, showing distinct seasonal variation. Biogenic particles (biogenic opal, particulate organic carbon, and calcium carbonate) account for about two thirds of annual total mass flux (49.2 g m^-2), among which biogenic opal flux is the most dominant (42% of the total flux). A positive relationship (except January) between biogenic opal and total organic carbon fluxes suggests that these two variables were coupled, due to the surface-water production (mainly diatoms). The relatively low δ¹³C values of settling particles result from effects on C-fixation processes at low temperature and the high CO² availability to phytoplankton. The correspondingly low δ¹⁵N values are due to intense and steady input of nitrates into surface waters, reflecting an unlikely nitrate isotope fractionation by degree of surface-water production. The δ¹⁵N and δ¹³C values of sinking particles increased from the beginning to the end of a presumed phytoplankton bloom, except for anomalous δ¹⁵N values. Krill and the zooplankton fecal pellets, the most important carriers of sinking particles, may have contributed gradually to the increasing δ¹³C values towards the unproductive period through the biomodification of the δ¹³C values in the food web, respiring preferentially and selectively¹²C atoms. Correspondingly, the increasing δ¹⁵N values in the intermediate-water trap are likely associated with a switch in source from diatom aggregates to some remains of zooplankton, because organic matter dominated by diatom may be more liable and prone to remineralization, leading to greater isotopic alteration. In particular, the tendency for abnormally high δ¹⁵N values in February seems to be enigmatic. A specific species dominancy during the production may be suggested as a possible and speculative reason.     

Ecotoxicological evaluation of sewage sludge using bioluminescent marine bacteria and rotifer

Bioassay using the marine bacteria,Vibrio fischeri and rotifer,Brachionus plicatilis, and chemical analyses were conducted to assess the toxicity of the various sewage sludges, one of the major ocean dumped materials in the Yellow Sea of Korea. Sludge elutriates extracted by filtered seawater were used to estimate the ecotoxicity of the sludge. Chemical characterization included the analyses of organic contents, heavy metals, and persistent organic pollutants in sludge. Bacterial bioluminescent inhibition (15 min), rotifer mortality (24 hr) and rotifer population growth inhibition (48 hr) assay were conducted to estimate the sludge toxicity. EC50 15 min (inhibition concentration of bioluminescence after 15 minutes exposed) values by Microtox® bioassay clearly revealed different toxicity levels depending on the sludge sources. Highest toxicity for the bacteria was found with the sludge extract from dyeing waste and followed by industrial waste, livestock waste, and leather processing waste. Clear toxic effects on the bacteria were not found in the sludge extract from filtration bed sludge and rural sewage sludge. Consistent with Microtox® results, rotifer neonate mortality and population growth inhibition test also showed highest toxicity in dyeing waste and low in filtration bed and rural sewage sludge. High concentrations of persistent organic pollutants (POPs) and heavy metals were measured in the samples from the industrial wastes, leather processing plant waste sludge, and urban sewage sludge. However, there was no significant correlation between pollutant concentration levels and the toxicity values of the sludge. This suggests that the ecotoxicity in addition to the chemical analyses of various sludge samples must be estimated before release of potential harmful waste in the natural environment as part of an ecological risk assessment.     

Progressive deformation of an evaporite-bearing accretionary complex: SeaMARC I,SeaBeam and piston-core observations from the Mediterranean Ridge

The Mediterranean Ridge is an arcuate ridge of deformed sediment caught up in the convergent plate margin between the African plate and the Aegean. An intensive campaign of SeaMARC I and SeaBeam surveys followed by piston coring has been conducted along the contact between undeformed turbidites of the Sirte Abyssal Plain and folded and faulted sediments of the Mediterranean Ridge. Along the outer edge of the Ridge, surficial sediments have been deformed into sinusoidal ridges and troughs (wavelengths 0.5–2 km, amplitude 20–150 m), which we interpret as folds. In plan view, the ridge and the trough fabric parallels the NW-SE trending regional contours, suggesting that the folds formed in response to compression orthogonal to the Mediterranean Ridge. The outermost ridge is shedding a debris apron out onto the abyssal plain, implying that uplift and deformation are ongoing. We show that the geometry of the outermost folds can be produced by elastic bending of a packet of 5–10 relatively strong layers, each 10–20 m thick, interbedded between weaker layers; we equate the strong layers with gypsum beds in the Messinian upper evaporites. Folding the seafloor from a flat layer into the observed ridge and trough topography would shorten the layer by less than 2%. Two percent shortening (equals two percent thickening) is insufficient to create the observed relief of the Mediterranean Ridge even if the entire sediment column down to basement were involved; we infer that additional shortening/thickening is accommodated by thrust faulting above a decollement at the top of the Messinian salt layer. At distances > 15 km from the deformation front and more than 500 m from the abyssal plain, sharp-edged, fine-grained side-scan lineations with very little vertical relief cut across the kilometer-scale ridge and trough topography. These fine-grained lineations fall in two groups trending N/S to NNE/SSW and ~ENE. We interpret these lineaments as traces of conjugate strike-slip faults formed in the same compressional regime which formed the NW/SE trending folds. The onset of strike-slip faulting may coincide with the cessation of imbricate thrust fan development above the initial salt-controlled decollement surface. The following characteristics of the Mediterranean Ridge are attributed to the presence of evaporites in the incoming sedimentary section: (1) initial deformation by folding rather than thrust faulting; (2) narrow taper; (3) rapid rate of outward growth; (4) karstification.