Identification of aryl hydrocarbon receptor 2 in aquatic birds; cDNA cloning of AHR1 and AHR2 and characteristics of their amino acid sequences

The toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and its related planar halogenated aromatic hydrocarbons (PHAHs) are mediated by the aryl hydrocarbon receptor (AHR). To investigate the potential sensitivity to PHAHs and the evolutional diversity of AHR in aquatic birds, AHR cDNAs were initially cloned and sequenced from the livers of a black-footed albatross (Diomedea nigripes) and a common cormorant (Phalacrocorax carbo). In this study, we report the identification of two distinct AHR paralog genes in these species. The two full-length AHR cDNAs from albatross were highly divergent (33% overall amino acid identity, and 60% identity in the N-terminal half). Phylogenetic analysis showed that one of them belongs to the AHR1 clade and the other one to the AHR2 clade, which has been identified only from fishes, but not yet from mammals and birds. Albatross AHR1 encoded a 861-residue protein with a predicted molecular mass of 96.7 kDa, and in the case of albatross AHR2, 925 amino acids and 100.7 kDa. From cormorant liver, the full-length AHR1 cDNA and the partial AHR2 cDNA were cloned. This result strongly suggests that bird species also possess two distinct AHR genes (AHR1 and AHR2). To our knowledge, this is the first report on the presence of an AHR2-like isoform in bird species as well as in fish.     

Water surface resonance in the L-shaped channel of seawater exchange breakwater

The resonance period of the L-shaped channel in the caisson is predicted analytically for the seawater exchange breakwater of “Applicability Study of the Seawater Exchange Breakwater (1). Korea Ministry of Maritime Affairs and Fisheries (in Korean) (1999a)”. Hydraulic experiments are conducted for a composite breakwater with a rear reservoir that is one of the seawater exchange breakwaters developed by them. For regular waves, the water surface elevation in the channel and the flow rate through the breakwater are measured. For irregular waves, the flow rate through the breakwater and the reflection coefficient on the breakwater are measured. The resonant maximum values in both the surface elevation and the flow rate, and the resonant minimum values in the reflection coefficient are all at wave periods slightly longer than analytically predicted ones. The measured resonance period for irregular waves is closer to the predicted one than for regular waves. If the resonance period of the L-shaped channel is fitted to the dominant period of incident waves, there would be high efficiency of seawater exchange between inside and outside the harbor.     

Numerical flow analysis of single-stage ducted marine propulsor

The present work has solved 3D incompressible RANS equations on a rotating, non-orthogonal multi-blocked grid to efficiently analyze a ducted marine propulsor with rotor–stator interaction. To handle the interface boundary between a rotor and a stator, the sliding multi-block technique using the cubic spline interpolation and the bilinear interpolation was applied. To validate the present code, the flow of a single stage turbine flow was simulated. Time averaged pressure coefficients were compared with experiments and good agreements were obtained. After the code validation, the flowfield around a single-stage ducted marine propulsor having a single stage of rotor and stator was successfully simulated and the hydrodynamic performance coefficients were computed.     

Geochemical-mineralogical-characteristics of the pre-Holocene sediments in Haenam Bay, west coast of Korea

The geochemical and mineralogical characteristics of pre-Holocene core sediments from Haenam Bay, southwest coast of Korea, were examined for post-depositional diagenetic changes and used to assess the provenance of fine-grained sediments in the region. The geochemical and mineralogical compositions of the sediments vary very little with core depth (Holocene to pre-Holocene age), except for the upper part of the pre-Holocene sedimentary layer. In this case, smectite and chlorite are depleted, presumably due to intensive subaerial weathering and low pH conditions. Highly mobile elements (Ca, Mg, Na and K) were likely removed with the breakdown of labile minerals, such as carbonate minerals, feldspar, and clay minerals, during subaerial exposure of this layer, resulting in low weathering indices. In particular, the low smectite content (<5%) and low V/Al ratio (<13) suggest that the Holocene and pre-Holocene sediments along the southwestern coast of Korea did not originate from mainland China but rather mostly from the Korean Peninsula. This aspect has been a subject of debate since the mid-1970s.     

Geochemical-mineralogical-characteristics of the pre-Holocene sediments in Haenam Bay, west coast of Korea

The geochemical and mineralogical characteristics of pre-Holocene core sediments from Haenam Bay, southwest coast of Korea, were examined for post-depositional diagenetic changes and used to assess the provenance of fine-grained sediments in the region. The geochemical and mineralogical compositions of the sediments vary very little with core depth (Holocene to pre-Holocene age), except for the upper part of the pre-Holocene sedimentary layer. In this case, smectite and chlorite are depleted, presumably due to intensive subaerial weathering and low pH conditions. Highly mobile elements (Ca, Mg, Na and K) were likely removed with the breakdown of labile minerals, such as carbonate minerals, feldspar, and clay minerals, during subaerial exposure of this layer, resulting in low weathering indices. In particular, the low smectite content (<5%) and low V/Al ratio (<13) suggest that the Holocene and pre-Holocene sediments along the southwestern coast of Korea did not originate from mainland China but rather mostly from the Korean Peninsula. This aspect has been a subject of debate since the mid-1970s.     

Variations in the abundance of fisheries resources and ecosystem structure in the Japan/East Sea

Evidence supports the hypothesis that two climatic regime shifts in the North Pacific and the Japan/East Sea, have affected the dynamics of the marine ecosystem and fisheries resources from 1960 to 2000. Changes in both mixed layer depth (MLD) and primary production were detected in the Japan/East Sea after 1976. The 1976 regime shift appears to have caused the biomass replacement with changes in catch production of major exploited fisheries resources, including Pacific saury, Pacific sardine and filefish. Both fisheries yield and fish distribution are reflected in these decadal fluctuations. In the 1960s and 1990s, common squid dominated the catches whereas in the 1970s and 1980s, it was replaced by walleye pollock. In the post-1988 regime shift, the distribution of horse mackerel shifted westward and southward and its distributional overlap with common mackerel decreased. The habitat of Pacific sardine also shifted away from mackerel habitats during this period. To evaluate changes in the organization and structure of the ecosystem in the Japan/East Sea, a mass-balanced model, Ecopath, was employed. Based on two mass-balanced models, representing before (1970–75) and after (1978–84) the 1976 regime shift, the weighted mean trophic level of catch increased from 3.09 before to 3.28 after. Total biomass of species groups in the Japan/East Sea ecosystem increased by 15% and total catch production increased by 48% due to the 1976 regime shift. The largest changes occurred at mid-trophic levels, occupied by fishes and cephalopods. The dominant predatory species shifted from cephalopods to walleye pollock due to the 1976 regime shift. It is concluded that the climatic regime shifts caused changes in the structure of the ecosystem and the roles of major species, as well as, large variations in biomass and production of fisheries resources.     

Imposex in the rock shell, Thais clavigera, as evidence of organotin contamination in the marine environment of Korea

Imposex was measured in the rock shell, Thais clavigera, from the coast of the Korean peninsula. Frequency of imposex was 0% at two reference sites, but at 47 out of 61 sites, frequency of imposex was recorded as 100%. The degree of imposex was relatively high at the sites near a harbor and a shipyard. Tributyltin (TBT) and triphenyltin (TPT) concentrations in T. clavigera ranged from 5 to 508 ng/g and from 3 to 2460 ng/g, respectively. A significant positive relationship was found between degree of imposex and organotin concentration, whereas a significant negative relationship was obtained between female-to-male sex ratio and the degree of imposex. During a field transplantation of T. clavigera from a pristine area to a port, TBT and TPT were accumulated in T. clavigera, and imposex was induced. T. clavigera shows considerable potential as a bioindicator species of the adverse effects of TBT and TPT contamination.     

Hull/mooring/riser coupled dynamic analysis and sensitivity study of a tanker-based FPSO

A computer program is developed for hull/mooring/riser coupled dynamic analysis of a tanker-based turret-moored FPSO (Floating Production Storage and Offloading) in waves, winds, and currents. In this computer program, the floating body is modeled as a rigid body with six degrees of freedom. The first- and second-order wave forces, added mass, and radiation damping at various yaw angles are calculated from the second-order diffraction/radiation panel program WAMIT. The wind and current forces for various yaw angles of FPSO are modeled following the empirical method suggested by OCIMF (Oil Company International Marine Forum).

The mooring/riser dynamics are modeled using a rod theory and finite element method (FEM), with the governing equations described in a generalized coordinate system. The dynamics of hull, mooring lines, and risers are solved simultaneously at each time step in a combined matrix for the specified connection condition. For illustration, semi-taut chain-steel wire-chain mooring lines and steel catenary risers are employed and their effects on global FPSO hull motions are investigated. To better understand the physics related to the motion characteristics of a turret-moored FPSO, the role of various hydrodynamic contributions is analyzed and assessed including the effects of hull and mooring/riser viscous damping, second-order difference-frequency wave-force quadratic transfer functions, and yaw-angle dependent wave forces and hydrodynamic coefficients. To see the effects of hull and mooring/riser coupling and mooring/riser damping more clearly, the case with no drag forces on those slender members is also investigated. The numerical results are compared with MARIN's wave basin experiments.