Growth of gametophytes and sporophytes of <Emphasis Type="Italic">Grateloupia subpectinata</Emphasis> (Rhodophyta) in culture

Comparison of growing thalli in alternating haploid and diploid phases of Grateloupia subpectinata (Rhodophyta) was studied. Fertile thalli from gametophyte and tetrasporophyte of G. subpectinata were collected from Yangyang, on the eastern coast of Korea. The size of the released tetraspores and carpospores was measured; the spores were then incubated at the temperature of 20°C, irradiance of 40 μmol photon m^-2s^-1 and photoperiod of 12L and 12D. Carpospores were also cultivated in the same conditions as the tetraspores culture. The crusts were subsequently transferred to a tank culture after six months. The specific growth rate (SGR) was measured by observing 50 crusts and 30 thalli. The released carpospores had a larger diameter (9.98 μm) than the tetraspores (9.38 μm). The crusts from the carpospores also show a higher specific growth rate (14.04% d^-1) than tetraspores (13.39% d^-1). After being transferred and cultured in a tank, the upright thalli grew slowly in May-June (13–15°C) and rapidly in July–September (17–22°C). The length of growing thalli of sporophyte from carpospores also revealed a higher specific growth rate (2.83% d^-1) than gametophytic thalli (2.38% d^-1). The specific growth rate of crusts and thalli developed from carpospores was higher than that of the crusts developed from tetraspores. This result suggests that the cultivation of sporophytes may be more profitable than gametophytes because harvesting can be done more efficiently.     

Boldness in a deep sea hermit crab to simulated tactile predator attacks is unaffected by ocean acidification

Despite rapidly growing interest in the effects of ocean acidification on marine animals, the ability of deep-sea animals to acclimate or adapt to reduced pH conditions has received little attention. Deep-sea species are generally thought to be less tolerant of environmental variation than shallow-living species because they inhabit relatively stable conditions for nearly all environmental parameters. To explore whether deep-sea hermit crabs (Pagurus tanneri) can acclimate to ocean acidification over several weeks, we compared behavioral “boldness,” measured as time taken to re-emerge from shells after a simulated predatory attack by a toy octopus, under ambient (pH ～7.6) and expected future (pH ～7.1) conditions. The boldness measure for crab behavioral responses did not differ between different pH treatments, suggesting that future deep-sea acidification would not influence anti-predatory behavior. However, we did not examine the effects of olfactory cues released by predators that may affect hermit crab behavior and could be influenced by changes in the ocean carbonate system driven by increasing CO₂ levels.     

Monitoring trace metals in seawater using a diffusive gradient in thin film probe in Ulsan Bay,East Sea,Korea: Comparison with transplanted mussels

To evaluate the applicability of a diffusive gradient in thin film (DGT) probe for monitoring dissolved metals in coastal seawater, DGT-labile metal concentrations were compared with total dissolved metal concentrations using spiked and natural seawater samples in the laboratory and transplanted mussels (Mytilus galloprovincialis). This was achieved through the simultaneous deployment of DGT probes and transplanted mussels in Ulsan Bay during winter and summer. DGT-labile metal concentrations were 45% (Cu) ~ 90% (Zn) of total dissolved concentrations, and the order of non-labile concentrations was Cu > Pb > Co ~ Ni > Cd ~ Zn in both metal-contaminated and non-contaminated seawater samples, which was similar to the order of stability of metal complexes in the Irving–Williams series. The overall variability of the DGT probe results within and between tanks was less than 10% (relative standard deviation: RSD) for all the metals tested during a 48-h deployment. The accumulation of metals, as determined by DGT probes, represented the spatial gradients better than the transplanted mussels did for all of the metals tested, and the extent of metal accumulation in mussels differed depending on the metal. The comparison of results for the DGT probe and the transplanted mussels in two seasons (winter and summer) suggested that metal accumulation in mussels was controlled by the physiological factors of mussels and partly by their diet (particulate metal loadings). The DGT probe could be used as a monitoring tool for dissolved metals in coastal seawater because its results explained only labile species. When using the DGT probe, slightly more than half of the total dissolved concentration in seawater samples for all the metals investigated displayed timeintegrated properties and distinct spatial gradients from pristine to metal-contaminated seawater.     

Mechanical and Germination Characteristics of Stabilized Organic Soils

High-organic-content dredged soils are known to have inferior mechanical characteristics because they are highly compressible and have low shear strength. To recycle dredged soil with a high organic content as a top soil this study describes an investigation of the mechanical properties and germination characteristics of stabilized organic soils using unconfined compression tests, pH tests, and seed germination tests. Several mixtures with organic contents in the range 0–30% by mass and binder contents in the range 5–15% were prepared to evaluate the effects of the organic content on the mechanical and germination characteristics of the stabilized soils. The results show that an increase in the organic content leads to a decrease in the strength and pH of the stabilized organic soil, which are favorable conditions for germination. The germination rate increased significantly with the increasing organic content, and the plant growth rate also increased. The addition of a binder into the mixtures increased the strength of the soil; however, it also increased the pH and decreased the rate of seed germination and plant growth.     

The Effect of Stabilizing Piles on a Self-Supported Earth-Retaining Wall

The behavior of a self-supported earth-retaining wall with stabilizing piles was investigated using a numerical study and field tests in urban excavations. Special attention is given to the reduction of lateral earth pressures acting on a retaining wall with stabilizing piles. Field tests at two sites were performed to verify the performance of the instrumented retaining wall with stabilizing piles. A number of 3D numerical analyses were carried out on the self-supported earth-retaining wall with stabilizing piles to assess the results stemming from wide variations of influencing parameters such as the soil condition, the pile spacing, the distance between the front pile and the rear pile, and the embedded depth. Based on the results of the parametric study, the maximum horizontal displacement and the maximum bending moment are significantly decreased when the retaining wall with stabilizing piles is used. In engineering practice, reducing the pile spacing and increasing the distance between the front pile and the rear pile can effectively improve the stability of the self-supported earth-retaining wall with stabilizing piles.     

Land response to atmosphere at different resolutions in the common land model over East Asia

Towards a better understanding of hydrological interactions between the land surface and atmosphere, land surface models are routinely used to simulate hydro-meteorological fluxes. However, there is a lack of observations available for model forcing, to estimate the hydro-meteorological fluxes in East Asia. In this study, Common Land Model (CLM) was used in offline-mode during the summer monsoon period of 2006 in East Asia, with different forcings from Asiaflux, Korea Land Data Assimilation System (KLDAS), and Global Land Data Assimilation System (GLDAS), at point and regional scales, separately. The CLM results were compared with observations from Asiaflux sites. The estimated net radiation showed good agreement, with r =0.99 for the point scale and 0.85 for the regional scale. The estimated sensible and latent heat fluxes using Asiaflux and KLDAS data indicated reasonable agreement, with r = 0.70. The estimated soil moisture and soil temperature showed similar patterns to observations, although the estimated water fluxes using KLDAS showed larger discrepancies than those of Asiaflux because of scale mismatch. The spatial distribution of hydro-meteorological fluxes according to KLDAS for East Asia were compared to the CLM results with GLDAS, and the GLDAS provided online. The spatial distributions of CLM with KLDAS were analogous to CLM with GLDAS, and the standalone GLDAS data. The results indicate that KLDAS is a good potential source of high spatial resolution forcing data. Therefore, the KLDAS is a promising alternative product, capable of compensating for the lack of observations and low resolution grid data for East Asia.     

Quantifying the uncertainty of the annular mode time scale and the role of the stratosphere

The impact of high resolution modern vegetation cover on the West African climate is examined using the International Centre for Theoretical Physics Regional Climate Model implementing the NCAR Community Land Model. Two high resolution 25 km long-term simulations driven by the output from a coarser 50-km resolution simulation are performed for the period 1998–2010. One high resolution simulation uses an earlier and coarser-resolution version of plant functional type distribution and leaf area index, while the other uses a more recent, higher-quality, and finer-resolution version of the data. The results indicate that the new land cover distribution substantially alters the distribution of temperature with warming in Central Nigeria, northern Gulf of Guinea and part of the Sahel due to the replacement of C4 grass with corn; and cooling along the coastlines of the Gulf of Guinea and in Central Africa due to the replacement of C4 grass with tropical broadleaf evergreen trees. Changes in latent heat flux appear to be largely responsible for these temperature changes with a net decrease (increase) in regions of warming (cooling). The improved land cover distribution also results in a wetter monsoon season. The presence of corn tends to favor larger precipitation amounts via more intense events, while the presence of tropical broadleaf evergreen trees tends to favor the occurrence of both more intense and more frequent events. The wetter conditions appear to be sustained via (1) an enhanced soil moisture feedback; and (2) elevated moisture transport due to increased low-level convergence in regions south of 10N where the most substantial land cover differences are present. Overall the changes induced by the improved vegetation cover improve, to some extent, the performance of the high resolution regional climate model in simulating the main West African summer monsoon features.