厚壳贻贝（Mytilus coruscus）McNF-κB基因的克隆及其在发育中的作用

核因子κB（Nuclear Factor kappa-B,NF-κB）具有免疫、炎症、凋亡、细胞增殖和发育的调节作用,目前NF-κB在脊椎动物和果蝇中的研究较为丰富,在贝类中的报道较少。为进一步探究NF-κB在厚壳贻贝（Mytilus coruscus）免疫和发育中的作用,本研究克隆了厚壳贻贝McNF-κB基因的序列全长,其全长为4 087 bp,开放阅读框为2 613 bp,编码871个氨基酸,具有典型的锚蛋白重复序列（ankyrinrepeat, ANK）结构域和死亡结构域。氨基酸序列分析结果发现,该基因与欧洲贻贝（Mytilus edulis）和地中海贻贝（Mytilus galloprovincialis）分别具有72.76%和66.58%同源性,且在系统进化树中与欧洲贻贝和地中海贻贝聚为一支。经实时荧光定量PCR（qRT-PCR）技术检验表明,McNF-κB基因于厚壳贻贝各组织均有分布,在鳃中表达最高;McNF-κB基因在厚壳贻贝眼点幼虫阶段和稚贝阶段均有表达,且在稚贝阶段表达量显著高于眼点幼虫阶段。利用RNA干扰技术沉默眼点幼虫McNF-κB基因后幼虫变态率显著下降,推测McNF-κB基因调控厚壳贻贝幼虫变态过程。本研究为探究McNF-κB基因如何调控厚壳贻贝发育奠定了基础。     

Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性,属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高,α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高,且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸,并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示,α螺旋和β片层围成了蛋白酶的催化腔,EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体,且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性,并有助于其催化功能的发挥,为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。     

最近十年来中国地理科学的进展

最近十年来中国地理科学的最重要进展有:自然地理学与人文地理学的平衡发展,人口、资源、环境的研究,遥感的应用与地理信息系统的建立,自然地理定位观测,青藏高原隆起对自然环境的影响,城市与城市规划,中国历史地理研究等。最近,并已开始研究气候变化与海平面上升对中国的影响,使我国地理科学研究紧紧跟上世界发展的步伐。     

硅硼镁铝矿－柱晶石－电气石组合在南极的发现

硅硼镁铝矿、柱晶石和电气石组合产于东南极拉斯曼丘陵，三种矿物相互接触并表现出特定的顺序：电气石（Ｔｒｎ１）→硅硼镁铝矿→电气石（Ｔｒｎ２）→柱晶石→电气石（Ｔｒｎ３），表明其产出是受Ｂ２Ｏ３、Ｈ２Ｏ活度的时间演化而不是空间变化所控制。这一组合的出现对于变质作用环境及过程中组分性质是一个很好的限定     

Exact solution of entanglement of the double Jaynes—Cummings model without rotating wave approximation

This paper investigates the influences of atom--field coupling and dipole--dipole coupling for atoms on the entanglement between two atoms by means of concurrence. The results show that the sudden death occurs when the atom--field coupling is strong enough, and the collapse and the revival appear when the dipole--dipole interaction is strong enough.     

外部利润、产权界定与土地资源优化配置——成都市农村土地股份合作制改革的制度经济学解析

以"外部利润——产权界定——资源优化配置"为研究主线,对成都市农村土地股份合作制改革进行了制度经济学解析。研究发现,制度创新空间的形成、土地的增值收益、土地规模利用效应和土地集约利用效应构成了成都市农村土地股份合作制改革的外部利润。成都市农村土地股份合作制改革,一方面可以提高土地规模和集约利用效应,促进土地资源优化配置;另一方面可以明晰集体土地产权主体,健全基层组织治理机制。要采取诸如放松法律限制、加强理论研究、规范机制建设等措施,从而推动土地股份合作制的生成和发展。     

The unification of RBLs with XBLs

Many astronomers have discussed the property of BL Lacertae objects, including the variation of spectrum, the correlation of multi-wave bands and the property of polarization, which could give good information for studying intrinsic correlation of components and position of BL Lacertae objects. In the paper, we investigated the properties of RBLs and XBLs and RBLs/XBLs (these objects can also be found by radio survey as by X-ray survey). Firstly, we collected the light curve of 28 BL Lacertae objects, and gained their short timescales by Structure Function (SF). Secondly, we analyzed the distribution of the short timescales of some BL Lacertae objects, including 28 objects’ timescales calculated by SF, as well as the distribution of the redshift and the black hole mass and the flux densities of the multi-wavebands (radio, near-infrared, optical and Gamma-ray). Based on statistical analysis, the result of the paper support the unification model of RBLs and XBLs.     

Galileo single frequency ionospheric correction: performances in terms of position

For GPS single frequency users, the ionospheric contribution to the error budget is estimated by the well-known Klobuchar algorithm. For Galileo, it will be mitigated by a global algorithm based on the NeQuick model. This algorithm relies on the adaptation of the model to slant Total Electron Content (sTEC) measurements. Although the performance specifications of these algorithms are expressed in terms of delay and TEC, the users might be more interested in their impact on positioning. Therefore, we assessed the ability of the algorithms to improve the positioning accuracy using globally distributed permanent stations for the year 2002 marked by a high level of solar activity. We present uncorrected and corrected performances, interpret these and identify potential causes for Galileo correction discrepancies. We show vertical errors dropping by 56–64 % due to the analyzed ionospheric corrections, but horizontal errors decreasing by 27 % at most. By means of a fictitious symmetric satellite distribution, we highlight the role of TEC gradients in residual errors. We describe mechanisms permitted by the Galileo correction, which combine sTEC adaptation and topside mismodeling, and limit the horizontal accuracy. Hence, we support further investigation of potential alternative ionospheric corrections. We also provide an interesting insight into the ionospheric effects possibly experienced during the next solar maximum coinciding with Galileo Initial Operation Capability.     

近代回归分析在交通调查分析建模中的应用

本文以出行发生量模型的建立为例,较为系统地讨论了近代回归分析中的自变量选择及回归诊断方法在交通调查分析建模中的应用。分析了在获取调查数据后,如何借助回归自变量选择方法来选择最佳自变量子集,以确定简捷的回归模型;文中应用回归诊断方法,讨论了修正回归模型、探测错误的调查数据的方法,从而为建立简捷、高精度的交通模型打下了基础。最后,作者提出了应用自变量选择及回归诊断方法建立出行发行量模型的一般步骤。     

1:50000DRG生产中有关问题的探讨

１： ５０ ０００ＤＲＧ生产技术经过近几年来的发展,已基本趋向于成熟。 １： ５０ ０００地形图经扫描、几何纠正及色彩归化,形成与 １： ５０ ０００地形图基本保持一致的栅格数据,即 ＤＲＧ。本文通过总结一年来的生产经验,对具体生产中的有关问题提出一些建议,仅供参考。