黄金新药源与豫西含金植物

黄金可以入药，历代本草均有记载。但因不溶性金被人体吸收极微，内服有一定毒性，加之其价格昂贵及一些药理学和比较复杂的检测处理与技术加工问题，不能普遍应用。自然界许多动植物都是聚金能手，特别是生长于富金岩石及金矿床风化土壤中的植物含金一般较高，部分动物因以含金植物为食，体内也含一定量的金，并且有些动植物本身就是中药材和食物。据此建议采取一定手段将含金较高、但未达工业品级的岩石，通过生物循环把金聚集至动、植物体内，金与动植物体内蛋白质等有机质结合成金的有机络合物，将此类动植物用于医疗或食疗，既可减小金的毒性，便于人体吸收，又可把未达工业品级的含金岩石利用起来。豫西某构造蚀变岩金矿，其上覆土壤中生长着含金植物，这些植物灰分中含金４×１０－９～９８×１０－９，其中以野苋菜的籽和叶灰分中含金最高（含金７５×１０－９～９８×１０－９），看来在本区用野苋菜籽和叶灰分中金含量进行找矿是可行的     

GPS接收机仪器偏差的长期变化特性分析

基于欧洲定轨中心2001-2009年发布的部分IGS测站接收机仪器偏差估值,分析了不同类型接收机DCB的长期变化特性.结果表明:各类接收机DCB的长期时变特征均具备一定的周期性,其中以月和年周期最为明显;接收机DCB的长期变化中还存在一定的波动,部分接收机DCB的稳定性随时间积累逐渐变差,甚至存在偏移.     

Fish assemblage structure in the hypoxic zone in the Changjiang (Yangtze River) estuary and its adjacent waters

Fish assemblage structure in the hypoxic zone in the Changjiang (Yangtze River) estuary and its adjacent waters were analyzed based on data from bottom trawl surveys conducted on the R/V Beidou in June, August and October 2006. Four fish assemblages were identified in each survey using two-way indicator species analysis (TWIA). High fish biomass was found in the northern part, central part and coastal waters of the survey area; in contrast, high fish diversity was found in the southern part of the survey area and the Changjiang estuary outer waters. Therefore, it is difficult to maintain high fishery production when high fish diversity is evenly distributed in the fish community. Fish became smaller and fish size spectra tended to be narrower because of fish species variations and differences in growth characteristics. Fish diversity increased, the age to maturity was reduced and some migrant species were not collected in the surveys. Fish with low economic value, small size, simple age structure and low tropic level were predominant in fish assemblages in the Changjiang estuary and its adjacent waters. The lowest hypoxic value decreased in the Changjiang estuary and its adjacent waters.     

Broader pattern of tandem repeats in the mitochondrial control region of Perciformes

Perciformes, the largest order of vertebrates with 20 suborders, is the most diverse fish order that dominates vertebrate ocean life. The complete mitochondrial control region (CR) of Trichiurus japonicus (Trichiuridae, Scombroidei) and Pampus sp. (Stromateidae, Stromateoidei) were amplified and sequenced. Together with data from GenBank, the tandem repeats in the mitochondrial CR from 48 species, which covered nine suborders of Perciformes, are reported in this study. The tandem repeats tend to be long in the suborder Percoidei and Stromateoidei. The identical repeats in 21 species of Cichlidae suggest a common origin and have existed before species divergence. Larimichthys crocea shows tandem repeats instead of the typical structure of the central conserved sequence blocks, which was first reported in Perciformes and vertebrates. This might have resulted from interruption of the polymerase activity during the H-strand synthesis. The four broader patterns presented here for the tandem repeats, including those in both the 5′ and 3′ ends, only in the either 5′ or 3′ end, and in the central conserved domain of the control region, will be useful for understanding the evolution of species.     

Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.     

2016年门源Ms6.4地震前祁连山周边断层活动分析

基于青藏高原东北缘地区最新的跨断层资料,利用时序曲线分析、断层速率合成分析和垂直形变累积率分析等多种方法进行计算,从不同角度和范围提取2016年门源Ms6.4地震震前异常特征,揭示断层活动。结果显示：1）震中距150 km以内多个场地出现了典型的以加速张性变化为主的短期异常,150~300 km内的异常以趋势变化为主;2）祁连山-海原断裂带上异常场地的空间分布表现出由祁连山断裂带中部集中向东迁移至中东部集中的特征,海原断裂上异常有所增多,此类现象与2003年民乐6.1级地震前的现象极其相似;3）祁连山断裂自2014年以来合成速率偏离正常动态值较远,容易积累应变。垂直形变趋势累积率的计算结果显示,地震震中位于形变积累的高值区。     

莱芜市农村土地确权登记问题与分析

莱芜市在全省率先启动了农村宅基地总登记,探索集体建设用地使用权抵押登记,在当前农村宅基地登记发证中存在着违法用地、产权不清晰、一户多宅、跨区域购房等问题,与宅基地使用权有效行使不相适应。因此,需加强宅基地的管理和登记的政策研究,制定可行的政策与措施,其中尤其要加强对"一户多宅"问题的处理。     

BDS区域卫星导航系统空间信号精度评估

针对BDS区域卫星导航系统的空间信号精度评估问题,分析利用精密星历进行空间信号评估的算法以及SISURE的投影原理,讨论BDS不同星座的参数选择。估计BDS各个卫星的空间信号精度,估算出SISURE综合误差的平均值为1.54 m,与GPS的空间信号精度还存在一定差距。针对个别导航卫星某些分量误差偏大的情况进行分析,发现GEO卫星SISURE个别时段较差和轨道机动有一定关系,并明确了BDS实际的空间信号精度,可为BDS用户提供一定参考。     

Biomarker records of phytoplankton productivity and community structure changes during the last 14000 years in the mud area southwest off Cheju Island, East China Sea

The ecological environment in the East China Sea (ECS) and the Yellow Sea (YS) has changed significantly due to sea-level rising and the Kuroshio incursion since the last deglaciation. In this study, biomarker records of core F10B from the mud area southwest off Cheju Island (MSWCI) were generated to evaluate phytoplankton productivity and community structure changes in response to environmental evolution during the last 14 kyr. The contents of diatom, dinoflagellate and haptophyte biomarkers (brassicasterol, dinosterol and C₃₇ alkenones) display similar trends, with increasing phytoplankton productivity during the last 14 kyr due to the increased influences of the Kuroshio, and especially due to the eddy-induced upwelling during the late Holocene. On the other hand, the contents of terrestrial biomarkers (C₂₈ +C₃₀ +C₃₂ n-alkanols) and terrestrial organic matter (TOM) proxies (TMBR’ and BIT) all reveal decreasing TOM input into the area around the sampling site for the 14 kyr, mostly due to sea-level rising. Phytoplankton biomarker ratios reveal a shift from a haptophyte-dominated community at 6.2–2.5 kyr BP to a diatom-dominated community at 2.5–1.45 kyr BP, likely caused by a stronger cold eddy circulation system at 2.5–1.45 kyr BP in the MSWCI.     

Effects of feeding time on complement component C7 expression in Pelteobagrus vachellii subject to bacterial challenge

Journal of Oceanology and Limnology - Shifting the feeding time for fish from daytime to nighttime could alter their digestive behavior, disturb their metabolism, and may affect immune-related...