Screening and molecular classification of low-temperature protease from Antarctic microorganism and its characterization

107 strains producing protease were screened from 260 strains of Antarctic psychrophilic bacteria, among which proteolytic activity of five strains was more than 45 U ml^-1. The 16S rRNA gcne sequences homology and phylogcnetic analysis of five Antarctic psychrophillc bacteria showed that NJ276, NJS-9, NJ16-70,NJ345 belonged tO the described genus Pseudoalteromonas and NJ341 belonged to the genus Colwellia. The growth and the protease characteristic of four Antarctic psychrophilic bacteria had been studied, and the result showed that the 6ptimal temperature for growth and protease-produeing of four strains was about 10℃. Their growth and protease-produeing were still high during incubatlng 2-5 days. The maximum proteolytic activity occurred at pH 9 for four Antarctic psychrophilic bacteria. The optimal temperature of protease action of both strains NJ276 and NJ5-9 was about 50℃, however, the optimal temperature of protease aetlon of both strains NJ341 and NJ345 was about 40 ℃, and their proteolytic activity under 0℃ exhibited nearly 30% of the maximum activity, but their thermal stabilities were weaker. These results indicated that proteases from NJ341 and NJ345 were low-temperature proteases.     

Displacement‐controlled method and its applications to material non‐linearity

For the analysis of non‐linear problems, the displacement‐controlled method (DCM) has a more extensive application scope and more powerful abilities than the load‐controlled method (LCM). However, difficulties of the DCM's procedure not amenable to most finite element implementations of the conventional LCM have restricted its applications in geomechanics. By means of Sherman–Morrison's theorem, the solution of DCM is improved. The improved procedure is characterized by high efficiency, good numerical stability and a programme structure similar to LCM. Two aspects of applications of DCM are illustrated. The first application is to compute the response of a structure under a given load level like the conventional finite element analysis. The second application is to trace the equilibrium path of a structure under a given load distribution type. A simple but effective algorithm is presented for automatically adjusting the step length in tracing the equilibrium path. Examples illustrate that the proposed procedures are suited for modelling complicated non‐linear problems in geomechanics. Copyright © 2005 John Wiley & Sons, Ltd.     

芝罘群岩石地层划分及区域对比

芝罘群分布于烟台市区北部芝罘岛、崆峒岛、担子岛等岛屿上,自下而上划分为老爷山组、兵营组及东口组.东口组为新建岩石地层单位.该群岩石组合为白云母钾长片麻岩、含电气石钾长石英岩、钾长石英岩、镜铁矿钾长石英岩、石英岩、白云母石英片岩夹黑云片岩、透辉变粒岩及大理岩等.其原岩为富钾、铁、硼的长英质碎屑岩夹粘土岩及富镁碳酸盐岩.兵营组单颗粒锆石U-Pb年龄为2171Ma,其成岩时代属古元古代早期.芝罘群区域上可与辽东地区古元古代榆树砬子群对比.     

西南极菲尔德斯半岛白垩纪晚期和第三纪早期岩石的古地磁学及其大地构造意义

采自菲尔德斯半岛白垩纪晚期和第三纪早期五个岩层单位的12个采点109块定向岩芯标本进行了系统的古地磁学测定,从中得知,55～45Ma时期,该区极位置与澳大利亚的同时期结果不同,它经历了大约20°～30°的南向水平移动与70°～80°的西向旋转,逐渐地构成了今日彼此相对位置的格架。文章绘制出南极洲的视极移曲线。     

Short time variability of the water masers in W51M

We report the results of a month long program of daily observationsconducted to study the short-term variability of the H₂O maseremission from W51M. During the period of our experiment, we observedsignificant changes in the flux density of some maser features, but no driftin the velocity of any feature. The flux density of a feature at 82.8 kms^-1 declined from 1140 Jy to 152 Jy over the month-long monitoringprogram. We tentatively conclude that this variation may be caused byenhanced interstellar scintillation.     

Type III Bursts Associated with Millisecond Spikes Which Occurred Within the Frequency Range from 230 to 300 MHz

The metric spectral data obtained at the Yunnan Observatory from July 1990 to December 1991 are analysed and some type III bursts associated with millisecond spikes are found. Their different morphologies reveal the relation between the type III bursts and the millisecond spikes. Based on the occurrence time and the characteristics of continuity and changes of the morphologies in the spikes and the type III bursts of two typical events, we verify that the acceleration region of the coronal electrons is located above the emitting region of the millisecond spikes and the type III bursts. The observations show that the interface frequency of a pair of type III bursts lies near 250 MHz. Finally the authors attempt to explain qualitatively the generating mechanism of the metric millisecond spike-type III bursts by means of the plasma hypothesis.     

不同约束对GPS观测网平差结果的影响

对卫星轨道和测站坐标的约束程度设计了 4种方案 ,对中国地壳运动观测网络10 18GPS周的基准站观测数据进行了试验研究。结果显示 :松驰轨道和紧约束 IGS站方案的结果与紧约束轨道和 IGS站方案的结果基本一致 ,认为这与先验坐标的精确度较高有关。其他方案之间存在较大的差别。最后讨论了大尺度 GPS监测网数据处理时参考基准的选择问题 ,认为一般情况下 ,应该使用全球解 H文件 ,把平差结果归算到全球参考框架中     

建筑地基与基坑变形监测刍议

随着城市建设的高速发展 ,高层、超高层建筑在原建筑物稠密区大量兴建 ,加之过量超采城市地下水 ,带来的地基与基坑变形问题越来越突出。为了防治建筑地基与基坑变形灾害 ,必须建立操作性较强的变形监测系统 ,及时作好预警、预报工作 ,使变形监测的方法、技术更加完善。     

论地质旅游资源

地质旅游资源是自然旅游资源的核心,以成因、物质基础、空间分布为基础,结合其形态特征、社会经济特征等因素对地质旅游资源进行了初步分类,并探讨了地质旅游的特色及发展前景.     

邓小平语言中的礼貌现象

《邓小平文选》是领导者语言的范例,存在大量的礼貌现象,体现着礼貌的思想。它们的运用可以给交际对方留面子,从而使交际活动更好地进行下去。以Brown和Levinson的面子保全论为理论基础,运用Leech的礼貌原则及六个次准则分析了《邓小平文选》领导语言中的各种礼貌的语言现象,其实现途径以及它的内在语用机制。这说明礼貌理论对文本交际活动也具有很强的阐释力。