白缘(鱼央)5S rDNA的扩增分析及其在染色体上的FISH定位

本文分别对雌雄白缘(鱼央)的5S rDNA进行了PCR扩增和序列分析,并采用双色荧光原位杂交(FISH)技术,以白缘(鱼央)的5S rDNA序列和小麦的45S rDNA为探针,对其在白缘(鱼央)雌雄中期染色体上进行了FISH定位研究。结果表明,白缘(鱼央)5S rDNA序列无雌雄差异;5S rDNA的保守区序列为117 bp;5S rDNA和45S rDNA分别被定位于白缘(鱼央)的性染色体和第5号染色体上。同时从GenBank中获得了22种鱼的5S rDNA,运用DNAman软件构建了23种鱼的系统发育树,对白缘(鱼央)的进化地位进行了初步研究。本研究为阐明白缘(鱼央)在鱼类系统进化中的位置、重复序列在脊椎动物性染色体上的分布状况以及与性别决定与分化的关系,提供了资料积累和分析依据。     

K型加强节点承载力及滞回性能分析

K型相贯节点区域容易出现主管管壁的局部屈曲与撕裂,可以采用主管与内套管之间夹层灌混凝土的方法进行加强。采用有限元软件ANSYS,对加强前后节点的静力极限承载力和滞回性能进行分析。结果表明:在主管与内套管之间夹层灌混凝土可有效改善节点的破坏模式,减小节点附近的应力集中问题,同时增强节点的刚度与承载力;无加强节点滞回曲线较为丰满,承载力下降相对平缓,但和加强节点比较,承载力相差明显;减小内套管的尺寸在一定程度上可以提高节点的极限承载力。     

地震氡观测仪计量检定系统与实验分析

本文介绍了地震氡观测仪计量检定系统（氡室）的建设背景及结构组成。氡室具有氡体积活度（氡浓度）实时监测、动态补氡、氡期望值可调、稳定性好等特点。在氡室调试实验中进行了氡室的漏气率实验及4次补氡实验,计算出氡室的氡漏气率为0.0001393Bq/min,氡发生率为23.35Bq/min。根据《测氡仪检定规程》（JJG 825—2013）的技术要求进行计量效能验证,表明氡室的氡体积活度（氡浓度）为800Bq/m³、1500Bq/m³、3000Bq/m³、6000Bq/m³和15000Bq/m³时,连续8h稳定性均优于5%;为6000Bq/m³时,72h稳定性优于5%,符合国家计量技术规范对氡室计量标准的要求。     

Study on the correlativity of earthquakes impact on cities

Introduction With rapid development and advancement of economy and society, lots of city groups or city belts with ex-tra-large cities as their centers have been formed in China. The regions these city groups lie in usually have well-developed economy, dense population, and are regional politics and culture centers. Some groups lie in the regions with high level of earthquake activity, such as the Surrounding Capital City Group with the centers of Bei-jing and Tianjin. Once a large earthqua…     

Residues and sources recognition of polychlorinated biphenyls in surface sediments of Jiaojiang Estuary, East China Sea

Twenty-three surface sediment samples were collected from Jiaojiang Estuary, East China Sea in order to assess the distribution, possible sources, and potential risk of polychlorinated biphenyls (PCBs) in the environment. Total concentrations of PCBs ranged from 4.93 to 108.79 ng g(-1) dry weight for all sampling stations, with a substantial higher concentration in the inner part of the estuary than the outer part of the estuary. PCB patterns in sediments from sites 1-8 were extremely dominated by tetra-PCBs, which probably due to the discharge of local industrial plants but not the expected input from the nearby e-waste recycling areas. Principal component analysis revealed that the sources of PCBs for sites 9-23 were associated with Aroclor 1248 or Aroclor 1221. However, physical migration and bio-degradation may also play a role on PCB distribution. Hazard assessment of PCBs indicated possible toxic potential, particularly in areas close to point sources.     

利用重叠加窗方式恢复地面运动

在频率域,利用地震记录反卷积恢复地面运动,由于FFT运算导致的谱泄漏与系统传递函数对高频与低频的放大效果会导致地面运动恢复失败,本研究采用重叠加窗方式,可避免单一加窗的时域失真,并减小频域谱泄漏。结果表明,恢复地面运动取得了较好效果。     

Study of a comprehensive assessment method of the environmental quality of soil in industrial and mining gathering areas

Industrial and mining activities have been recognized as major sources of heavy metal contamination in soil. Here, we developed a comprehensive assessment method for the soil environment in industrial and mining gathering areas based on the pressure–state–response model. Using this method, we assessed the environmental quality of soil in a typical industrial and mining gathering area in Tianjin City, China. The results are as follows: (1) The comprehensive environmental quality index of the soil in the study area was 0.532, which corresponds to an alert state and shows that the soil environment is generally poor. (2) The pressure, state, and response indexes were 0.609, 0.634, and 0.163, respectively, which suggests that the pressure in the soil environment of the study area is barely acceptable, and the state is merely passable. Furthermore, the response measures are not ideal. (3) The low response index scores indicate poor production processes, low pollutant treatment level, and unsatisfactory level of management by the enterprises in the study area. (4) The distribution of soil risks was found to be inseparably related to that of contamination sources and land use types. Furthermore, the distribution was uneven to a certain degree. Finally, we propose recommendations for the optimization, adjustment, and management of typical industrial and mining gathering areas with petrochemical, metallurgy, and other heavily polluting enterprises.     

塔里木北部地区三叠系储层溶蚀作用机理

塔里木北部地区三叠系储层在成岩演化过程中曾经历两期溶蚀作用，深埋成岩环境的晚期溶蚀较早期近地表－浅埋成岩环境溶蚀要剧烈得多，溶蚀作用的最大特点是铝硅酸盐矿物较碳酸盐矿物剧烈。溶蚀作用发生的机理是由于三叠系储层处于８０～１２０℃热窗、成岩流体富含低分子量羧酸、碱度由低分子量羧酸控制，铝硅酸盐和碳酸盐矿物均处于去稳定状态。溶蚀作用的发育程度受控于储层砂体的沉积和岩石学特征以及早期成岩发育程度。     

重金属Cu、Pb对泥鳅(Misgurnus anguillicaudatus)卵细胞凋亡及DNA损伤的SCGE试验

采用室内暴露试验方法,以单细胞凝胶电泳技术(SCGE)检测,研究了Cu、Pb不同浓度梯度与不同暴露时间联合染毒对泥鳅卵细胞DNA的损伤.结果表明,各Cu、Pb浓度组DNA平均迁移长度增加,与阴性对照组比较差异有显著性(P＜0.05).此外,随着Cu、Pb染毒剂量的增加,各试验组DNA的平均迁移长度逐渐增加,在试验浓度梯度范围内(Cu 0.01mg/L+Pb 0.05mg/L、Cu 0.10mg/L+Pb 0.50mg/L、Cu 0.25mg/L+Pb 0.75mg/L),存在较为显著的剂量-效应关系(P＜0.05),但未见明显的时间-效应关系(P＞0.05).Cu、Pb可引起泥鳅卵细胞凋亡和DNA损伤,卵细胞的不同损伤水平可望作为较为理想的水环境基因毒性指标.     

老168平台海油陆采丛式井组关键钻井工艺

老168平台丛式井组是2009年中石化及胜利油田的重点产能建设项目之一,平台上共部署58口海油陆采定向井。该丛式井组的施工主要有以下难点：井网密集,防碰工作量大;造斜点浅、井斜大、造斜困难;位移大、裸眼稳斜段长,稳斜难度大;钻井液技术必须考虑环境保护问题,可借鉴经验非常少。因此钻井施工难度极大,通过实施优化井场布局及井眼轨道技术、大井眼松软浅地层定向技术、高精度轨迹控制技术、大位移井安全钻进技术,优质无污染海水钻井液技术,最终实现了老168钻完井一体化作业,节约了成本,提高了效率,为今后胜利油田丛式井组施工提供了宝贵经验。