Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography

In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.     

坚持土地节约集约利用促进文登经济和谐发展

文登市位于山东半岛东部,总面积1 779.84km2。辖14个镇、3个街道办事处、2处省级开发区,810个行政村、88个居委会,总人口64.25万人,其中农村人口46.2万人。全市耕地面积62 137.93hm2,人均耕地不足0.084 hm2,低于全国和全省的平均水平,土地供需矛盾日益突出。     

淄博市淄川区治理私开滥挖工作经验

淄川区位于淄博市中部,是一个具有百年开采历史的工矿老区,全区面积1 000 km2,辖17个乡镇、3个街道、1个省级经济开发区,总人口67.3万人。境内矿产资源丰富、储量大、矿种多、分布广、埋藏浅、易开采以及综合利用价值高,已累计发现煤、铁、耐火黏土、陶瓷土、石灰石等矿种24种。截至2008年底,全区共有矿山企业126处,从业人员12 000余人,年产各类矿产品1 150余万吨,实现工业总产值84 000余万元,矿业为淄川区经济社会发展做出了重大贡献。但由于受经济利益驱动,私开滥挖曾一度屡禁不止,严重影响了正常开采,2008年底,淄川区被省政府列为资源枯竭型城市。     

一种获取Klobuchar电离层改正模型的新方法

介绍了利用松弛搜索法对Klobuchar模型的参数进行拟合,并利用IGS提供的IONEX格式对全球电离层数据进行试验分析,获得了可靠的结果。     

基于GPU的数字影像的正射纠正技术的研究

本文简述了CUDA编程模型高性能并行计算的特性,在实现了基于GPU的数字影像正射纠正的基础上,阐述了基于GPU的加速技术在数字影像处理方面的应用情景.     

Rossby waves with linear topography in barotropic fluids

Rossby waves are the most important waves in the atmosphere and ocean, and are parts of a large-scale system in fluid. The theory and observation show that, they satisfy quasi-geostrophic and quasi-static equilibrium approximations. In this paper, solitary Rossby waves induced by linear topography in barotropic fluids with a shear flow are studied. In order to simplify the problem, the topography is taken as a linear function of latitude variable y, then employing a weakly nonlinear method and a perturbation method, a KdV （Korteweg-de Vries） equation describing evolution of the amplitude of solitary Rossby waves induced by linear topography is derived. The results show that the variation of linear topography can induce the solitary Rossby waves in barotropic fluids with a shear flow, and extend the classical geophysical theory of fluid dynamics.     

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菏泽市土地利用存在的问题及潜力分析

1土地利用特点 泽市土地总面积121.94万hm2,已利用土地115.28万hm2,土地利用率为94.54%,农用土地97.99万hm2,占土地总面积的80.36%,其中耕地83.30万hm2,占土地总面积的.68.26%,园地1.22万hm2,占土地总面积的1.00%,林地6.38万hm2,占土地总面积的5.22%;其他农用地7.24万hm2,占土地总面积的5.88%;建设用地18.93万hm2,占土地总面积的15.52%;未利用土地5.35万hm2,占土地总面积的4.12%.     

利用QuikSCAT/NCEP混合风场及WAVEWATCH模拟东中国海风浪场

将国际上比较成熟的海浪模式WAVEWATCH-Ⅲ移植到东中国海,采用QuikSCAT卫星遥感资料与NCEP数据混合风场作为强迫项,对该海区2000年1月23日至31日的一个大风天气过程下的风浪场进行了模拟计算。计算结果与浮标观测资料的对比结果说明,所获得的风浪场数值结果不但具有较高的时空分辨率,其精度也是比较高的。