Iron Regulation of Wetland Vegetation Performance Through Synchronous Effects on Phosphorus Acquisition Efficiency

Iron-rich groundwater flowing into wetlands is a worldwide environmental pollution phenomenon that is closely associated with the stability of wetland ecosystems. Combined with high phosphorus(P) loading from agricultural runoff, the prediction of the evolution of wetland vegetation affected by compound contamination is particularly urgent. We tested the effects of anaerobic iron-rich groundwater discharge in a freshwater marsh by simulating the effect of three levels of eutrophic water on native plants(Glyceria spiculosa(Fr. Schmidt.) Rosh.). The management of wetland vegetation with 1–20 mg/L Fe input is an efficient method to promote the growth of plants, which showed an optimum response under a 0.10 mg/L P surface water environment. Iron-rich groundwater strongly affects the changes in ecological niches of some wetland plant species and the dominant species. In addition, when the P concentration in a natural body of water is too high, the governance effect of eutrophication might not be as expected. Under iron-rich groundwater conditions, the δ~(13)C values of organs were more depleted, which can partially explain the differences in δ~(13)C in the soil profile. Conversely, the carbon isotope composition of soil organic carbon is indicative of past changes in vegetation. The results of our experiments confirm that iron-rich groundwater discharge has the potential to affect vegetation composition through toxicity modification in eutrophic environments.     

Expression and enzymatic characterization of a cold-adapted β-agarase from Antarctic bacterium Pseudoalteromonas sp.NJ21

An agar-degrading bacterium,designated as Pseudoalteromonas sp. NJ21,was isolated from an Antarctic sediment sample. The agarase gene a ga1161 from Pseudoalteromonas sp. NJ21 consisting of a 2 382-bp coding region was cloned. The gene encodes a 793-amino acids protein and was found to possess characteristic features of the Glyco_hydro_42 family. The recombinant agarase(r Aga1161) was overexpressed in Escherichia coli and purified as a fusion protein. Enzyme activity analysis revealed that the optimum temperature and pH for the purified recombinant agarase were 30–40°C and 8.0,respectively. rAga1161 was found to maintain as much as 80% of its maximum activity at 10°C,which is typical of a coldadapted enzyme. The pattern of agar hydrolysis demonstrated that the enzyme is an β-agarase,producing neoagarobiose(NA2) as the final main product. Furthermore,this work is the first proof of an agarolytic activity in Antarctic bacteria and these results indicate the potential for the Antarctic agarase as a catalyst in medicine,food and cosmetic industries.     

Replacement of Dietary Fish Oil with Vegetable Oils Improves the Growth and Flesh Quality of Large Yellow Croaker （Larmichthys crocea）

We investigated the effect of the replacement of dietary fish oil with vegetable oils on the growth and flesh quality of large yellow croaker(Larmichthys crocea). The basal diet(FO) was formulated to contain 66.5% fish meal and 6.4% menhaden fish oil; whereas the other 3 experimental diets were formulated by replacing the fish oil with 50% soybean oil(SO50), 100% soybean oil(SO100) and 100% palm oil(PO100), respectively. The 4 diets were randomly assigned to 4 floating sea cages(3.0 m × 3.0 m × 3.0 m), and each was stocked with 250 fish individuals with an initial average weight of 245.29 g ± 7.45 g. The fish were fed to apparent satiation twice a day at 5:00 and 17:00, respectively, for 12 weeks. Experimental analysis showed that the specific growth rate of fish fed SO50 or PO100 were significantly higher than that of fish fed FO or SO100(P0.05), and crude lipid contents of ventral muscle and viscera were significantly lower in fish fed FO than in those fed the other 3 diets(P0.05). No significant differences in condition factor, viscerosomatic index, hepatosomatic index, gutted yield and colorimetric values of fish among the dietary treatments were observed(P0.05). Compared to FO diet, SO50, SO100 and PO100 diets led to substantial decreases in the liquid loss and water loss from fresh fillets(1 d, 4℃)(P0.05). Similarly, thiobarbituric acid reactive substance(TBARS) values of fillets under different storage conditions(1 d, 4℃; 7 d, 4℃; 4 weeks,-20℃; 8 weeks,-20℃) decreased significantly after partial or complete replacement of fish oil with vegetable oils. These findings indicated that the growth performance and selected flesh quality properties(liquid holding capacity and TBARS value) of large yellow croaker were substantially improved by replacing dietary fish oil with vegetable oils.     

The potential of ocean acidification on suppressing larval development in the Pacific oyster Crassostrea gigas and blood cockle Arca inflata Reeve

We evaluated the effect of pH on larval development in larval Pacific oyster （Crassostrea gigas） and blood cockle （Arca inflata Reeve）. The larvae were reared at pH 8.2 （control）, 7.9, 7.6, or 7.3 beginning 30 min or 24 h post fertilization. Exposure to lower pH during early embryonic development inhibited larval shell formation in both species. Compared with the control, larvae took longer to reach the D-veliger stage when reared under pH 7.6 and 7.3. Exposure to lower pH immediately after fertilization resulted in significantly delayed shell formation in the Pacific oyster larvae at pH 7.3 and blood cockle larvae at pH 7.6 and 7.3. However, when exposure was delayed until 24 h post fertilization, shell formation was only inhibited in blood cockle larvae reared at pH 7.3. Thus, the early embryonic stages were more sensitive to acidified conditions. Our results suggest that ocean acidification will have an adverse effect on embryonic development in bivalves. Although the effects appear subtle, they may accumulate and lead to subsequent issues during later larval development.     

云南前寒武纪基底形成与变质时代及其成矿作用年代学研究

新的同位素年代学、岩石学与地球化学研究表明云南前寒武纪各岩群普遍由跨越古、中和新元古代不同时代的构造片岩组成.哀牢山群和瑶山群是相连接的同一地层单元,主体岩石形成于1 300~1 600 Ma.昆阳群下亚群泥质碳酸盐沉积岩Pb-Pb等时年龄在1 700~1 600 Ma之间,上亚群大营盘组为1 260 Ma.苍山群的变拉斑系列形成于岛弧环境,其钕模式年龄值在1 500~2 000 Ma,Sm-Nd等时年龄近于为1 650 Ma,εNd(t)=+7.4.形成于板内环境的碱性系列玄武岩,钕模式年龄值在1 000~1 100 Ma.苴林群变拉斑玄武岩钕模式年龄为1 600~1 700 Ma,εNd(t)=+6.6;变碱性玄武岩样品为800~1 000 Ma,可与苍山群相比较.钕模式年龄和Pb同位素填图表明昆阳群与大红山群,苴林群与苍山群分别归属于扬子和印支块体的准同时地层.成矿年龄测定表明前寒武纪地层中成矿作用表现为700~900 Ma的改造成矿,与大规模从基性到酸性的岩浆活动密切相联系.900~1000 Ma板内环境碱性玄武岩的广泛出现,标致着大陆裂解的开始.晋宁期花岗岩活动从早期(800~830 Ma)的偏中酸性到晚期(680~780 Ma)的酸性,表明构造环境从拉张向挤压环境发展,体现了Rodinia超大陆形成、裂解到冈瓦纳大陆形成的过程.     

渤海湾西岸全新世海面变化

在讨论海面标志点基本定义的基础上,从渤海湾西岸534个原始数据中获得了136个指示高水位、潮间带或低水位的海面变化标志点,并重建了它们的相对海平面时空分布,进而获得了渤海湾西岸全新世相对海平面变化带.约10cal.ka B.P. 时,相对海平面变化带达到-25m,以约6m/1000a(即约6mm/a)的平均速率上升,在6cal.ka B.P. 前后达到现代海面的高度; 6cal.ka B.P. 至今,变化带的高度介于+1m~-2m之间,未发现中全新世相对高海面.再搬运海相贝类和陆相泥炭类样品的 14C 年龄,分别存在约600年和660年的驻留时间.经过驻留时间校正的新海面变化年代学序列,将渤海湾相对海平面达到现代高度的时间点确定为约6cal.ka B.P.,从而与全球海面变化的对比更为准确.渤海湾盆地的长期稳定下沉和沉积自重压实的共同作用,可能抵消了冰川均衡调整(GIA)引起的中全新世数米高的相对高海面.     

丹江口水库叶绿素a浓度的时空特征及影响因子分析

2017年5月—2019年10月,对"南水北调"中线水源水库——丹江口水库库区水体7个监测位点、0～20 m间4个水层的垂向叶绿素a (Chl.a)浓度与水质因子进行了季度性调查,以期了解不同位点、不同水层Chl.a分布的主要驱动因子.结果表明,丹江口水库各位点营养状态指数(TSI)均为中营养化状态.水体Chl.a浓度具有逐年增加的趋势,且极高值有逐渐增加的趋势.营养盐和Chl.a浓度均存在较大的空间异质性,入库区具有较高的总磷和氨氮浓度,汉江库区具有最高的Chl.a浓度,源头污染源控制和监测仍然是丹江口水库管理的重中之重.不同位点Chl.a浓度的驱动因子存在较大差异,汉江入库和大坝区Chl.a浓度分别受到硝态氮和p H的影响,而出水口大坝位点主要受到了水深、水温和氨氮的影响.丹江入库区Chl.a浓度受到了水深、氨氮、总磷和水温的影响,但丹江库区表现出了与其他生态区较大的区别,其Chl.a浓度主要受到水深和有机质输入的影响.因此,对丹江口水库各位点的管理,应该分不同生态区采取针对性的管理措施.本研究旨在为南水北调中线工程可持续的生态调度提供基础生态数据支持,为完善水库水源地的有效管理提供理论支撑.     

基于构造的测震学参数在鲁东-黄海地块的应用

以基于构造的测震学参数分析为思路,利用地震构造分区和地质构造单元的划分结果,检验了基于活动边界带的测震学参数方法的可行性;将活动地块内部（地质构造复杂且地震活动频繁）构造单元与测震学参数相结合,研究中强震前震源区所在的构造单元测震学参数的变化特征,提取具有中期预测意义的震兆标志,为中强震的预测提供理论依据。结果显示：中强地震前,郯庐断裂带（鲁东-黄海地块西边界带）及扬子地块（鲁东－黄海地块内部构造单元）D、C值异常过程明显,且各有特点,证明了基于活动地块内部构造单元的测震学参数方法具有良好的可行性。     

黔西南高原区峰丛洼地的形态特征与发育规律

本文通过对黔西南坡岗地区峰丛洼地形态参数的统计和趋势面分析，论证了高原区峰丛洼地的形态特征与发育，探讨了高原区暗河纵剖面形态和喀斯特地貌演化的规律。