高纯河豚毒素对蛙坐骨神经动作电位的影响

用电生理学方法观察了4种不同浓度的高纯河豚毒素(tetrodotoxin,TTX)对蛙离体坐骨神经动作电位的影响.实验结果表明:1μmol/dm3TTX在给药后7 min能显著抑制动作电位的上相幅度(P<0.05),但在给药后10 min才显著抑制动作电位的下相幅度(P<0.01);20μmol/dm3TTX在给药后3 min内能完全抑制动作电位的形成.1μmol/dm3TTX在给药后1~5min对动作电位传导速度的影响没有统计学意义,5μmol/dm3TTX在5min时可显著抑制传导速度(P<0.05),而10μmol/dm3TTX在2min时便显著抑制传导速度(P<0.05).实验结果提示高纯河豚毒素对蛙离体坐骨神经动作电位的影响存在剂量效应关系.     

Species composition and distribution of marine nematode community in the North Taiwan Strait

I~IOW~ne nelnatode is a ban gIOup of benthic OrgAnsrns. Abbot 5 000 spotes Of free-livingnelnatedes have ben dedbo so far, and it has ho estimated that abbot 20 (XX) are as yetunknoWn. Chinese bothologist has Paid attention to marine nelnatodes in China seas in yare. The spatial distribution and shoes commotion of marine nelnatedes in the HUanghe EstUary, Qinhuangdao waters, QingdaO Bay and Xiamen ~ have ho reported (Zhang etal., 1990, 1991, 1993; ac and Li, 1998). ~ new shoes in the…     

南海海温异常与ENSO的相关性

使用1958－1987COADS资料，应用复经验正交函数（CEOF）分析方法，分析南海海表面温度场（SST）和风场（u及v）。结果发现，南海海温异常基本独立于西太平洋，同时存在类似于ENSO事件的年际变化，ENSO发生前冬季南海有异常降温过程，之后有增暖事件发生。分析还表明，南海SST异常主要取决于经向风场的异常强迫。南海SST与ENSO事件的相关性实质上反映了季风异常对ENSO循环的影响。     

浙北地区旱涝预报问题探讨—一种汛期降水量长期预报方法

本文分析了当地前期气象要素——温度的演变特点与汛期降水量之间的关系,建立了两者之间的回归方程,并由此作出降水量的长期预报,从预报误差和试报结果看,效果是好的。     

南海地区海气环境场的季节和年际变化

本文对南海地区(0—20°N,105—135°E)海气环境场进行EOF分析。其结果除了深入了解南海地区气候的基本场外,还揭示了叠加在基本场上的各种扰动场与热带天气系统的联系,为研究南海ITCZ等热带天气系统以及南海环境场对EL—Ni(?)o现象的反映提供了研究依据。     

长江口及济州岛邻近海域温、盐、密度的跃层现象

本文根据CTD观测资料,分析了研究海区的温、盐、密度跃层的分布与变化,讨论了逆温逆盐层的分布区域,并从跃层角度出发,分析了深层水的涌升,黄海冷水团的上边界以及台湾暖流在东海北部的影响范围。     

海藻硫酸多糖911对小鼠脾淋巴细胞增殖反应及对细胞产生IL—2作用的影响

本文以~3H—TdR掺入法观察911对小鼠脾淋巴细胞增殖反应的影响并用CTLL细胞检测了其对IL—2的作用。体外实验结果表明,911对小鼠脾淋巴细胞增殖反应有明显的增强作用,以0.5μg/ml的浓度效果最为明显,相对增殖指数RPI可达200％;体内实验则以5mg/kg体重ip,连续7d效果最好,相对增殖指数RPI可达176％;911用药组小鼠IL—2的产生量均高于对照组,以5mg/kg和10mg/kg效果最好。以上实验证明,这一多糖是一种有希望的新免疫调节药物。     

Overtopping Discharge of Wind-Waves on Simple Slope Dikes

An empirical formula for estimating the overtopping discharge of wind-waves on a smooth-impermeable-simple slope dyke is derived through model tests in this paper, it can be adopted by related design departments in the determination of the crest elevation of the dyke.     

渤海湾海河口区耗氧有机物的分布特征

渤海湾海河口区是北京、天津二大城市工业及生活污水排放入海处，是一比较典型的海域。1972年以来曾对渤海湾进行过综合性的环境污染调查和研究，但由于受调查范围和项目的限制，对河口区特别是大沽口、北塘口区有机污染物的迁移转化研究甚少，尚不足以就有机污染对河口生态环境的潜在影响作出科学判断。目前，有机污染已成为海河口区特别是其附近大城市的主要污染问题之一。本文以化学耗氧量(COD)、生物耗氧量(BOD5)、溶解氧(DO)等项目作为耗氧有机物污染程度评价的主要指标(还有氮、磷、本集另有专文讨论)，对海河口区有机污染物的迁移、转化及生态效应进行了调查研究，为有机污染评价提供数据并作初步探讨。     

Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.