Broader pattern of tandem repeats in the mitochondrial control region of Perciformes

Perciformes, the largest order of vertebrates with 20 suborders, is the most diverse fish order that dominates vertebrate ocean life. The complete mitochondrial control region (CR) of Trichiurus japonicus (Trichiuridae, Scombroidei) and Pampus sp. (Stromateidae, Stromateoidei) were amplified and sequenced. Together with data from GenBank, the tandem repeats in the mitochondrial CR from 48 species, which covered nine suborders of Perciformes, are reported in this study. The tandem repeats tend to be long in the suborder Percoidei and Stromateoidei. The identical repeats in 21 species of Cichlidae suggest a common origin and have existed before species divergence. Larimichthys crocea shows tandem repeats instead of the typical structure of the central conserved sequence blocks, which was first reported in Perciformes and vertebrates. This might have resulted from interruption of the polymerase activity during the H-strand synthesis. The four broader patterns presented here for the tandem repeats, including those in both the 5′ and 3′ ends, only in the either 5′ or 3′ end, and in the central conserved domain of the control region, will be useful for understanding the evolution of species.     

Advanced glycation endproducts in 35 types of seafood products consumed in eastern China

Advanced glycation endproducts(AGEs) have been recognized as hazards in processed foods that can induce chronic diseases such as cardiovascular disease, diabetes, and diabetic nephropathy. In this study, we investigated the AGEs contents of 35 types of industrial seafood products that are consumed frequently in eastern China. Total fluorescent AGEs level and Nε-carboxymethyl-lysine(CML) content were evaluated by fluorescence spectrophotometry and gas chromatography-mass spectrometry(GC-MS), respectively. The level of total fluorescent AGEs in seafood samples ranged from 39.37 to 1178.3 AU, and was higher in canned and packaged instant aquatic products that were processed at high temperatures. The CML content in seafood samples ranged from 44.8 to 439.1 mg per kg dried sample, and was higher in roasted seafood samples. The total fluorescent AGEs and CML content increased when seafood underwent high-temperature processing, but did not show an obvious correlation. The present study suggested that commonly consumed seafood contains different levels of AGEs, and the seafood processed at high temperatures always displays a high level of either AGEs or CML.     

A public Cloud-based China’s Landslide Inventory Database (CsLID): development,zone, and spatiotemporal analysis for significant historical events, 1949-2011

Landslide inventory plays an important role in recording landslide events and showing their temporal-spatial distribution. This paper describes the development, visualization, and analysis of a China's Landslide Inventory Database (CsLID) by utilizing Google’s public cloud computing platform. Firstly, CsLID (Landslide Inventory Database) compiles a total of 1221 historical landslide events spanning the years 1949-2011 from relevant data sources. Secondly, the CsLID is further broken down into six zones for characterizing landslide cause-effect, spatiotemporal distribution, fatalities, and socioeconomic impacts based on the geological environment and terrain. The results show that among all the six zones, zone V, located in Qinba and Southwest Mountainous Area is the most active landslide hotspot with the highest landslide hazard in China. Additionally, the Google public cloud computing platform enables the CsLID to be easily accessible, visually interactive, and with the capability of allowing new data input to dynamically augment the database. This work developed a cyber-landslide inventory and used it to analyze the landslide temporal-spatial distribution in China.     

Development of three multiplex PCR primer sets for ark shell (<Emphasis Type="Italic">Scapharca broughtonii</Emphasis>) and their validation in parentage assignment

Scapharca broughtonii is a commercially important and over-exploited species. In order to investigate its genetic diversity and population structure, 43 novel polymorphic microsatellites were isolated and characterized. The number of alleles per locus ranged from 3 to 22 with an average of 6.93, and the observed and expected heterozygosities varied between 0.233 and 1.000, and 0.250 and 0.953, with an average of 0.614 and 0.707, respectively. Three highly informative multiplex PCRs were developed from nine of those microsatellites for S. broughtonii. We evaluated and validated these multiplex PCRs in 8 full-sib families. The average polymorphism information content (PIC) was 0.539. The frequency of null alleles was estimated as 3.13% of all the alleles segregation based on a within-family analysis of Mendelian segregation patterns. Parentage analysis of real offspring demonstrated that 100% of all offspring were unambiguously allocated to a pair of parents based on 3 multiplex sets. Those 43 microsatellite loci with high variability will be helpful for the analysis of population genetics and conservation of wild stock of S. broughtonii. The 3 sets of multiplex PCRs could be an important tool of pedigree reconstruction, population genetic analysis and brood stock management.     

用数学形态学变换实现空间分析中的数据处理

论述了数学形态学的基本算法, 并将这一方法引入空间分析的数据处理中。研究表明,该方法具有一定的可行性, 对于空间分析中一些问题的深入研究具有参考价值     

Electrochemical corrosion behavior of hot dip coating in artificial seawater

INTR0DUCnONAlloycoatingscandetivelyproteCtsteelagainstcormsioninseri0uscormsionenvir-orunent,suchasthedrinesplashandtidalzones-TwoInainhodiptincaldrinumalloycOaingswidelyusedcommroiallyareGalfancoaing(contalningZn-5wt%kl-us)devel-oPedbytheIntemaionaltalZincReenhheZaionandhocoating(c0n-talningZn-55Wt%Al-1.6Wt%Si)develoPedbytheBethlehemSteelCorpOration-Anewrinc-basedalloycOaingdevel0PedbyNortheaStemUniversityisdiscussedinthesPaperbyelecthehendcalmethod.FromSun(l996),welmOWthecorms…     

MONITORING POLYNUCLEAR AROMATIC HYDROCARBONS IN SEDIMENT POREWATER BY SPMD

A new mimic biological Semi-permeable Membrane Device (SPMD) introduced for sampling organic pollutants yielded satisfactory results when it was frrst used as a passive sampler to concentrate and determine 16 kinds of polynuclear aromatic hydrocarbons (PAHs) by means of capillary GC on an HP 5890 GC-FID in coastal sediment perewater. The concentration of PAHs in sediment porewater for naphthalene(N), acenaphthlene(AL), acenaphthene (AE), fluorene (F), phenaphthene(P), anthracene(A), fluoranthene(FA), pyrene(Py), benzo[a]anthracene(B[a]A), chrysene(Chr), benzo[b] fluor- anthene(B[b]F), benzo[k]fluoranthene(B[k]F), benzo[a]pyrene(B[a]P),indeno[1,2,3,-cd]-Pyrene(I[123]P), dibenz[a,h]anthracene(D[ab]A) and benzo[g,h,i] perylene(B[ghi]P) were:50.36, under detection limits(UD), 18.19, 8.41, 8.40, 1.44, UD, 8.01, 524.15, 168.47, 50.13,123.66, 63.48, 27.40, 82.04 and 58,81 ng/L, respectively.     

花岗岩在剪切带中定位机制的几个实例——赴英考察报告节摘

本文介绍了不列颠群岛地质背景和花岗岩在剪切带中定位机制的几个实例,即Donegal、Strantian,Ox山脉花岗岩和Ardnamurchan中心杂岩体。它是作者访英考察报告中的主要部分,也是英国花岗岩及其定位机制的最新成果。对我们了解花岗岩研究的现代水平,具有借鉴作用。     

鄂尔多斯盆地苏里格南部地区盒8段沉积相特征及其意义

依据野外露头、岩芯、测井及相关测试资料,对鄂尔多斯盆地苏里格南部地区上古生界中二叠统石盒子组盒8沉积期的沉积类型、沉积微相特征及沉积相对天然气成藏富集的影响进行了分析。结果表明:鄂尔多斯盆地苏里格南部地区盒8沉积期自北向南依次发育缓坡浅水辫状河三角洲和滨浅湖沉积相,亚相主要为三角洲平原、三角洲前缘、滨湖和浅湖,微相包括分流河道、分流间洼地、水下分流河道、水下天然堤、分流间湾、滨湖砂坝、滨湖泥湾和浅湖泥湾;各微相在空间上相互叠置,复合加积;各微相水动力条件的差异造成微相在沉积物成分和组构上各异,导致其成岩作用不同;分流河道和水下分流河道微相发育处密集形成相互叠置的大面积骨架砂体,构成区内主砂带,成为天然气优质储集层,是有利勘探相带;沉积相明显控制优质储集层的分布,进而影响着天然气的富集成藏。总之,水下分流河道和分流河道微相是砂岩优质储集层分布和发育的最有利相带,也是今后勘探开发的方向。     

日照市东南部地区榴辉岩矿矿石加工技术性能及选矿工艺研究

日照市东南部岚山安东卫、虎山地区位于苏鲁榴辉岩带南西段的中部,区域内赋存大型榴辉岩矿体,榴辉岩资源较丰富。榴辉岩含有的石榴子石、绿辉石、金红石都是应用很广的矿物,但榴辉岩的选矿工艺比较复杂,特别是其中金红石的选矿分离一直难以解决。该文就日照东南部地区的榴辉岩矿石的加工技术性能及选矿工艺进行了试验和探讨研究,为今后该区榴辉岩矿的开发利用打下了一定基础。