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Broader pattern of tandem repeats in the mitochondrial control region of Perciformes

Perciformes, the largest order of vertebrates with 20 suborders, is the most diverse fish order that dominates vertebrate ocean life. The complete mitochondrial control region (CR) of Trichiurus japonicus (Trichiuridae, Scombroidei) and Pampus sp. (Stromateidae, Stromateoidei) were amplified and sequenced. Together with data from GenBank, the tandem repeats in the mitochondrial CR from 48 species, which covered nine suborders of Perciformes, are reported in this study. The tandem repeats tend to be long in the suborder Percoidei and Stromateoidei. The identical repeats in 21 species of Cichlidae suggest a common origin and have existed before species divergence. Larimichthys crocea shows tandem repeats instead of the typical structure of the central conserved sequence blocks, which was first reported in Perciformes and vertebrates. This might have resulted from interruption of the polymerase activity during the H-strand synthesis. The four broader patterns presented here for the tandem repeats, including those in both the 5′ and 3′ ends, only in the either 5′ or 3′ end, and in the central conserved domain of the control region, will be useful for understanding the evolution of species.     

内标法测定多金属元素分析条件的研究

文章通过对内标元素的选择,研究了分析信号与内标信号之间的相关性,选择合理的操作参数,大幅度地提高了分析精密度。     

Identification and sequence of a cDNA clone corresponding to a gene involved in development ofUndaria pinnatifida

During the induction of gamete-producing gametangia, induced gametophytes were collected at 4 days intervals (0,4,8,12 d) and total RNAs were isolated by CsCl gradient ultracentrifugation. Some stage-specific expressed mRNAs were identified by differential display of mRNAs from different developing stages of the gametophytes. The cDNA of one specific mRNA was verified, cloned and sequenced. This gene was specifically expressed during 4 days of induction, and had partial homologous sequence with tobacco IAA-binding protein gene. It suggests that this cDNA may represent a gene which is related to the IAA regulating function during the development of the gametophytes.

     

储良广眼砧穗不亲和问题研究

储良广眼的砧穗不亲和现象在众多个体中呈现从亲和至不亲和的连续变化，随砧、穗周长差异的增大，不亲和症状表现突出。砧、穗周长比值与几个生长量指标存在显著相关。提出以砧／穗比作为衡量不亲和程度指标。靠接加砧结果表明对严重不亲和树出现的症状有较大的缓解。     

鄂尔多斯盆地苏里格南部地区盒8段沉积相特征及其意义

依据野外露头、岩芯、测井及相关测试资料,对鄂尔多斯盆地苏里格南部地区上古生界中二叠统石盒子组盒8沉积期的沉积类型、沉积微相特征及沉积相对天然气成藏富集的影响进行了分析。结果表明:鄂尔多斯盆地苏里格南部地区盒8沉积期自北向南依次发育缓坡浅水辫状河三角洲和滨浅湖沉积相,亚相主要为三角洲平原、三角洲前缘、滨湖和浅湖,微相包括分流河道、分流间洼地、水下分流河道、水下天然堤、分流间湾、滨湖砂坝、滨湖泥湾和浅湖泥湾;各微相在空间上相互叠置,复合加积;各微相水动力条件的差异造成微相在沉积物成分和组构上各异,导致其成岩作用不同;分流河道和水下分流河道微相发育处密集形成相互叠置的大面积骨架砂体,构成区内主砂带,成为天然气优质储集层,是有利勘探相带;沉积相明显控制优质储集层的分布,进而影响着天然气的富集成藏。总之,水下分流河道和分流河道微相是砂岩优质储集层分布和发育的最有利相带,也是今后勘探开发的方向。     

日照市东南部地区榴辉岩矿矿石加工技术性能及选矿工艺研究

日照市东南部岚山安东卫、虎山地区位于苏鲁榴辉岩带南西段的中部,区域内赋存大型榴辉岩矿体,榴辉岩资源较丰富。榴辉岩含有的石榴子石、绿辉石、金红石都是应用很广的矿物,但榴辉岩的选矿工艺比较复杂,特别是其中金红石的选矿分离一直难以解决。该文就日照东南部地区的榴辉岩矿石的加工技术性能及选矿工艺进行了试验和探讨研究,为今后该区榴辉岩矿的开发利用打下了一定基础。     

The Impact of Irrigation on Bacterial Community Composition and Diversity in Liaohe Estuary Wetland

In this study, the sequencing of 16S ribosomal DNA was used to characterize the soil bacterial community composition and diversity in Liaohe estuarine wetland. Soil samples were taken from different locations in the wetland dominated by reed. Moreover, the soil quality parameters were evaluated (pH, moisture, organic matter, total nitrogen, available nitrogen, total phosphorus, available phosphorus). The results showed that the organic matter and nutrient contents were significantly higher in irrigated wetland than those in natural wetland. Major phylogenic groups of bacteria in soil samples including Proteobacteria, Acidobacteria, Gemmatimonadetes, Actinobacteria and Cyanobacteria were analyzed and we found that Proteobacteria was the most abundant in the community, and the phylum Acidobacteria was more abundant in irrigated wetland. Beta diversity analyses indicated that the soil bacterial community was mainly affected by sampling sites rather than seasons. In general, the bacterial community in natural wetland was not significantly different with that in artificial irrigated wetland. Artificial hydraulic engineering irrigated according to the water requirement rule of reed, increased the production of reeds, changed the way of wetland soil material input, but the diversity of bacterial community kept stable relatively.     

OGC WCS系统若干关键实现技术研究

由于栅格数据种类繁多,处理过程复杂,目前还没有一种软件能为他们提供完善的WCS。研究表明,针对具体的数据源设计和实现WCS是比较好的解决方案。研究针对MODIS数据,参照OGC WCS实现规范,在充分分析WCS数据处理与服务流程的基础上,总结了WCS系统实现的技术要点,研究和探讨了系统实现中的若干关键技术问题,包括元数...     

卫星双向法时间比对的归算

卫星双向法比对原理为两个台站同步发送和接收时间信号，可消除传递路径误差，因此比对精度高，它的缺点是效率低，占用大量卫星时间，也不适合于自动化操作，现正在研制的终端可多台站同时观测，克服了上述缺点，利用新的终端提出一种新的处理方法，研究表明多台站观测的同时性有更多的信息可提取，以提高比对精度。