Use of Chemotaxonomy To Study the Influence of Benzalkonium Chloride on Bacterial Populations in Biodegradation Testing

Contradictory results are reported for the behaviour of quaternary ammonium compounds (QACs) in sewage treatment plants (STPs). QACs may sorb onto activated sludge. Only little information is available with respect to effects of QACs against bacteria in STPs. Only 5 to 15 % of bacteria present in sewage sludge can be detected by means of culture dependent microbiological methods. The shift of the bacterial populations due to effects of test compounds have not been studied up to now with culture independent methods. The microbial populations shift was studied in situ using culture independent chemotaxonomy profiling ubiquinones and polyamines. Additionally, toxic effects of QACs against bacteria present in the test vessels of the Zahn‐Wellens test (OECD 302 B) were assessed with a toxicity control in the test. The ubiquinone profiles representing changes in Gram‐negative populations mainly showed that the activated sludge was affected only in test vessels containing benzalkonium chloride. According to chemotaxonomy Acinetobacter or/and some members of Pseudomonas spp. have been selected by benzalkonium chloride after some adaptation period (8 to 12 days).     

Cretaceous isochron ages from K–Ar and Ar / Ar dating of eclogitic rocks in the Tso Morari Complex, western Himalaya, India

The Tso Morari Complex, which is thought to be originally the margin of the Indian continent, is composed of pelitic gneisses and schists including mafic rock lenses (eclogites and basic schists). Eclogites studied here have the mineral assemblage Grt + Omp + Ca-Amp + Zo + Phn + Pg + Qtz + Rt. They also have coesite pseudomorph in garnet and quartz rods in omphacite, suggesting a record of ultrahigh-pressure metamorphism. They occur only in the cores of meter-scale mafic rock lenses intercalated with the pelitic schists. Small mafic lenses and the rim parts of large lenses have been strongly deformed to form the foliation parallel to that of the pelitic schists and show the mineral assemblages of upper greenschist to amphibolite facies metamorphism. The garnet–omphacite thermometry and the univariant reaction relations for jadeite formation give 13–21 kbar at 600 °C and 16–18 kbar at 750 °C for the eclogite formation using the jadeite content of clinopyroxene (X_Jd = 0.48).

Phengites in pelitic schists show variable Si / Al and Na / K ratios among grains as well as within single grains, and give K–Ar ages of 50–87 Ma. The pelitic schist with paragonite and phengite yielded K–Ar ages of 83.5 Ma (K = 4.9 wt.%) for paragonite–phengite mixture and 85.3 Ma (K = 7.8 wt.%) for phengite and an isochron age of 91 ± 13 Ma from the two dataset. The eclogite gives a plateau age of 132 Ma in Ar/Ar step-heating analyses using single phengite grain and an inverse isochron age of 130 ± 39 Ma with an initial ⁴⁰Ar / ³⁶Ar ratio of 434 ± 90 in Ar/Ar spot analyses of phengites and paragonites. The Cretaceous isochron ages are interpreted to represent the timing of early stage of exhumation of the eclogitic rocks assuming revised high closure temperature (500 °C) for phengite K–Ar system. The phengites in pelitic schists have experienced retrograde reaction which modified their chemistry during intense deformation associated with the exhumation of these rocks with the release of significant radiogenic ⁴⁰Ar from the crystals. The argon release took place in the schists that experienced the retrogression to upper greenschist facies metamorphisms from the eclogite facies conditions.     

Cd(II), Pb(II) and Zn(II) Removal from Contaminated Water by Biosorption Using Activated Sludge Biomass

Biosorption using activated sludge biomass (ASB) as a potentially sustainable technology for the treatment of wastewater containing different metal ions (Cd(II), Pb(II) and Zn(II)) was investigated. ASB metal uptake clearly competed with protons consumed by microbial biomass compared with control tests with non‐activated sludge biomass. Biosorption tests confirmed maximum exchange between metal ions and protons at pH 2.0–4.5. It was revealed by the study that the amount of metal ions released from the biomass increased with biomass sludge concentration. The result showed that maximum absorption of metal ions was observed for Cd(II) at pH 3.5, Pb(II) at pH 4.0, and pH 4.5 for Zn(II) ions. The maximum absorption capacities of ASB for Cd(II), Pb(II) and Zn(II) were determined to be 59.3, 68.5 and 86.5%, respectively. The biosorption of heavy metals was directly proportional to ASB stabilization corresponding to a reduction in heavy metals in the order of Cd < Pb < Zn. The order of increase of biosorption of metal ions in ASB was Zn(II) < Pb(II) < Cd(II), and this was opposite to that of non active sludge. The results indicate that ASB is a sustainable tools for the bioremediation of Cd(II), Pb(II) and Zn(II) ions from industrial sludge and wastewater treatment plants.     

Comparative analysis of Lake Urmia and Lake Van water level time series

Recent decline in Lake Urmia water level makes it crucial to consider this issue more seriously. For this purpose, comparison of water level in Lake Urmia with Lake Van in Turkey, which is in relatively similar geographic and climate conditions, can be an effective approach. To follow this objective, trend analysis, regime shift, and coherency analyses are implemented. The results showed negative trend in Lake Urmia water level for the past 20 years, while in Lake Van, the trend is positive. Moreover, correlation of the lake level variations versus the basin rainfall during three common periods, identified by the regime shifts analysis, illustrated a decreasing trend in the correlation. These changes can be attributed to non-climatic factors such as different allocation disciplines in two lake’s catchments. Finally, the coherency analysis showed significant annual and inter-annual frequencies common between the two lake levels. Herein, the short-term period relations are associated with lags, while in long term, they act simultaneously.     

Petrogenesis and tectonic setting of bimodal volcanism in the Sakoli Mobile Belt, Central Indian shield

The Sakoli Mobile Belt comprises bimodal volcanic rocks that include metabasalt, rhyolite, tuffs, and epiclastic rocks with metapelites, quartzite, arkose, conglomerate, and banded iron formation (BIF). Mafic volcanic rocks are tholeiitic to quartz‐tholeiitic with normative quartz and hypersthene. SiO₂ shows a large compositional gap between the basic and acidic volcanics, depicting their bimodal nature. Both the volcanics have distinct geochemical trends but display some similarity in terms of enriched light rare earth element–large ion lithophile element characteristics with positive anomalies for U, Pb, and Th and distinct negative anomalies for Nb, P, and Ti. These characteristics are typical of continental rift volcanism. Both the volcanic rocks show strong negative Sr and Eu anomalies indicating fractionation of plagioclases and K‐feldspars, respectively. The high Fe/Mg ratios for the basic rocks indicate their evolved nature. Whole rock Sm–Nd isochrons for the acidic volcanic rocks indicate an age of crystallization for these volcanic rocks at about 1675 ± 180 Ma (initial ¹⁴³Nd/¹⁴⁴Nd = 0.51017 ± 0.00017, mean square weighted deviate [MSWD] = 1.6). The εNd_t (t = 2000 Ma) varies between ?0.19 and +2.22 for the basic volcanic rock and between ?2.85 and ?4.29 for the acidic volcanic rocks. Depleted mantle model ages vary from 2000 to 2275 Ma for the basic and from 2426 to 2777 Ma for the acidic volcanic rocks, respectively. These model ages indicate that protoliths for the acidic volcanic rocks probably had a much longer crustal residence time. Predominantly basaltic magma erupted during the deposition of the Dhabetekri Formation and part of it pooled at crustal or shallower subcrustal levels that probably triggered partial melting to generate the acidic magma. The influence of basic magma on the genesis of acidic magma is indicated by the higher Ni and Cr abundance at the observed silica levels of the acidic magma. A subsequent pulse of basic magma, which became crustally contaminated, erupted as minor component along with the dominantly acidic volcanics during the deposition of the Bhiwapur Formation.     

Sequence stratigraphic analysis of Eocene Rock Strata,Offshore Indus,southwest Pakistan

In this study, seismic data from two wells (Pak G2-1 and Indus Marine-1C) and age diagnostic larger benthic foraminifera (LBF) within drill cuttings has been used for the first time to identify depositional sequences within the carbonates in the Offshore Indus Basin, Pakistan. The Offshore Indus is tectonically categorized as a passive continental margin where carbonates occur as shelf carbonates in the near offshore and on volcanic seamounts in deeper waters. Seismic data analysis has indicated the presence of minor faults and carbonate buildups above the igneous basement in the south. Patterns of the seismic reflections enabled definition of three seismic facies units identified as: Unit 1 basement, represented by chaotic, moderate amplitude reflection configuration; while parallel bedding and the drape of overlying strata is typical character of Unit 2, carbonate mound facies. The younger Miocene channels represent Unit 3. The diagnosis of Alveolina vredenburgi/cucumiformis biozone confirmed the Ilerdian (55–52 Ma) stage constituting a second order cycle of deposition for the Eocene carbonates (identified as Unit 2). The carbonate succession has been mainly attributed to an early highstand system tract (HST). The environmental conditions remained favorable leading to the development of keep-up carbonates similar to pinnacle buildups as a result of aggradation during late transgressive system tract and an early HST. The carbonate sequence in the south (Pak G2-1) is thicker and fossiliferous representing inner to middle shelf depths based on fauna compared to the Indus Marine-1C in the north, which is devoid of fossils. Three biozones (SBZ 5, SBZ 6 and SBZ 8) were identified based on the occurrence of LBF. The base of the SBZ 5 zone marks the larger foraminifera turnover and the Paleocene–Eocene (P–E) boundary. The LBF encountered in this study coincides with earlier findings for the P–E boundary. Our findings indicate that the entire Ilerdian stage ranges from 55.5 to 52 Ma that was the episode of warmer water conditions on the carbonate shelves leading to the diversification of K-strategist larger foraminifera. The larger foraminiferal assemblage encountered in this study confirms the findings. The possible indication of stratigraphic-combination traps, revealed as reflection terminations, make carbonate mounds in the south a potential exploration target.     

Geologic and hydrologic settings for development of freshwater lenses in arid lands

Satellite observations were used to test the validity of previously identified favourable conditions for the formation of freshwater lenses, identify additional potential occurrences, and model modern potential recharge in the Raudhatain Watershed (3696) in northern Kuwait. Favourable conditions include infrequent yet intensive precipitation events, drainage depressions to collect the limited runoff, and presence of conditions (e.g. high infiltration capacity) that promote groundwater recharge and preservation (e.g. underlying saline aquifer) of infiltrating groundwater as freshwater lenses floating over saline aquifer water due to differences in density. Specifically, the following field and satellite‐based observations were noted for the Raudhatain Watershed: (1) Over ~30 precipitation events were identified from the Tropical Rainfall Measuring Mission precipitation data (1998–2009); (2) slope is gentle (2 m/km), and the surface is largely (80%) covered by alluvial deposits with high infiltration capacities (up to 9 m/day); (3) no flows and long‐term ponding were reported at the watershed outlet or detected from Landsat thematic mapper images; (4) infiltration is high based on increases in soil moisture content (from an advanced microwave scanning radiometer) and vegetation index following large precipitation events; and (5) freshwater lenses that overlie highly saline [total dissolved solids (TDS): >35 000] unconfined aquifers underlying the watershed are absent in the southern regions, where infiltrating fresh water mixes with the less saline groundwater (TDS: <10 000). Twenty potential locations (size: 1 to 75 km²) for freshwater lens development were identified in northern Kuwait, and continuous rainfall–runoff models (Soil Water and Assessment Tool) were applied to provide a first‐order estimation of the average annual recharge in the watershed (127 × 10⁶ m³) and freshwater lenses (8.17 × 10⁶ m³). Results demonstrate the settings for enhanced opportunities for groundwater recharge, outline the amounts of and preservation conditions for the groundwater feeding the freshwater lenses, and highlight potential applications and locations of freshwater lenses in similar settings elsewhere in the Arabian Peninsula and beyond. Copyright © 2013 John Wiley & Sons, Ltd.     

New chelating resin for preconcentration and determination of molybdenum by inductive couple plasma atomic emission spectroscopy

A chelating resin is prepared by condensation polymerization of aniline with formaldehyde and characterized by Fourier transform infrared spectrometer, elemental analysis and thermogravimetric analysis and studied for the preconcentration and determination of trace Molybdate ion from environmental water sample using inductive couple plasma atomic emission spectroscopy. The optimum pH value for sorption of the metal ion was 5. The sorption capacity of functionalized resin is 3.1 mg/g. The chelating sorbent can be reused for 20 cycles of sorption-desorption without any significant change in sorption capacity. The best desorption of the metal ions from resin was obtained by 0.5 mol/L nitric acid as eluting agent. The profile of molybdenum uptake on this sorbent reflects good accessibility of the chelating sites in the aniline-formaldehyde. Scatchard analysis revealed that the homogeneous binding sites were formed in the polymers. The equilibrium adsorption data of Molybdate ion modified resin were analyzed by five isotherm models such as Langmuir, Freundlich and Temkin. Langmuir isotherm parameters obtained from the four Langmuir linear equations by using linear method. Based on the Langmuir isotherm analysis, the monolayer adsorption capacity was determined to be 4.03 mg/g at 20 °C. The method was applied for molybdenum ions determination from river water sample.     

The application of geochemical pattern recognition to regional prospecting: A case study of the Sanandaj–Sirjan metallogenic zone,Iran

In regional exploration programs, the distribution of elements in known mineral deposits can be used as a guide for the classification of deposits, search for new prospects and modeling ore deposit patterns. The Sanandaj–Sirjan Zone (SSZ) is a major metallogenic zone in Iran, containing lead and zinc, iron, gold, copper deposits. In the central part of the SSZ, lead and zinc mineralization is widespread and hitherto exploration has been based on geological criteria. In this study, we used clustering techniques applied to element distribution for classification lead and zinc deposits in the central part of the SSZ. The hierarchical clustering technique was used to characterize the elemental pattern. Elements associated with lead and zinc deposits were separated into four clusters, encompassing both ore elements and their host rock-forming elements. It is shown that lead and zinc deposits in the central SSZ belong to two genetic groups: a MVT type hosted by limestone and dolomites and a SEDEX type hosted by shale, volcanic rocks and sandstone. The results of elemental clustering were used for pattern recognition by the K-means method and the respective deposits were classified into four distinct categories. K-means clustering also reveals that the elemental associations and spatial distribution of the lead and zinc deposits exhibit zoning in the central part of the SSZ. The ratios of ore-forming elements (Sb, Cd, and Zn) vs. (Pb and Ag) show zoning along an E–W trend, while host rock-forming elements (Mn, Ca, and Mg) vs. (Ba and Sr) show a zoning along a SE–NW trend. Large and medium deposits occur mainly in the center of the studied area, which justify further exploration around occurrences and abandoned mines in this area. The application of a pattern recognition method based on geochemical data from known mineralization in the central SSZ, and the classification derived from it, uncover elemental zoning, identify key elemental associations for further geochemical exploration and the potential to discover possible target areas for large to medium size ore deposits. This methodology can be applied in a similar way to search for new ore deposits in a wide range of known metallogenic zones.