天降血栓通丸对2型糖尿病大鼠血糖、大血管病变的影响

目的：研究天降血栓通丸对2型糖尿病大鼠血糖、大血管病变的影响。方法：将90只wistar大鼠随机分为正常组15只与造模组75只,通过高糖高脂饲料+链脲佐菌素诱导建立糖尿病大鼠模型,造模成功后将其随机分为模型组,天降血栓通丸早期干预组、中期干预组、晚期干预组（简称为早期组、中期组、晚期组）,盐酸吡格列酮组（西药组）,每组各15只,分别采用相应药物干预。观察大鼠一般情况,检测空腹血糖、胰岛素、胰岛素敏感指数、细胞间黏附分子-1（ICAM-1）水平。结果：造模组与空白组比较,胰岛素、胰岛素敏感指标、体质量、第13周空腹血糖差异均有统计学意义（P＜0.01或P＜0.05）;药物干预后空腹血糖水平ICAM-1模型组、各治疗组与空白组比较,各治疗组与模型组比较,差异均有统计学意义（P＜0.05）。结论：天降血栓通丸无降糖作用,其早期干预能有效降低ICAM-1,这可能是其对2型糖尿病大鼠大血管病变影响的机制之一。     

消栓饮对创伤性深静脉血栓大兔Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）亚群漂移的影响

目的：探讨Th1（CD4^{+TNF-α+）/Th2（CD4+IL-4+）亚群漂移在大兔创伤性深静脉血栓（DVT）形成中的作用及消栓饮的治疗机制。方法：将45只新西兰健康大兔分为空白组、假手术组、0.9%氯化钠注射液组、低分子肝素钠组、消栓饮组,每组各9只,采用直接钳夹静脉+双后肢石膏固定的方法制备创伤性DVT模型后进行给药,于第1、3、7天完成给药4h后,采用流式细胞术检测各组大兔Th1（CD4+TNF-α+）、Th2（CD4+IL-4+）的含量变化及Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）值。结果：给药第1、3、7天0.9%氯化钠注射液组、低分子肝素钠组、消栓饮组的Th1（CD4+TNF-α+）比例、Th2（CD4+IL-4+）比例及Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）值与同时间节点空白组、假手术组比较,差异均有统计学意义（P<0.05）;给药第3、7天低分子肝素钠组、消栓饮组的Th1（CD4+TNF-α+）比例、Th2（CD4+IL-4+）比例及Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）值与0.9%氯化钠注射液组比较,差异均有统计学意义（P<0.05。结论：DVT形成过程中存在Th1（CD4+TNF-α+）/Th2（CD4+IL-4+）亚群动态平衡被打破;中药消栓饮可以通过干预Th1（CD4+TNF-α+）、Th2（CD4+IL-4+）含量变化,维持两者的平衡来达到治疗目的。}     

Effects of stocking density on the growth and immunity of Atlantic salmon salmo salar reared in recirculating aquaculture system(RAS)

Atlantic salmon reared in recirculating aquaculture system(RAS) may lead to inappropriately high stocking density, because ?sh live in a limited space. Finding the suitable stocking density of Atlantic salmon reared in RAS is very important for RAS industry. In this paper, the in?uence of stocking density on growth and some stress related physiological factors were investigated to evaluate the ef fects of stocking density. The ?sh were reared for 220 days at ?ve densities(A: 24 kg/m^3; B: 21 kg/m^3; C: 15 kg/m^3; D: 9 kg/m^3 and E: 6 kg/m^3). The results show that 30 kg/m^3 might be the maximum density which RAS can af ford in China. The stocking densities under 30 kg/m^3 have no ef fect on mortality of Atlantic salmon reared in RAS. However, the speci?c growth rate(SGR), ?nal weight and weight gain in the high density group were signi?cantly lower than the lower density groups and middle density groups. Moreover, feed conversion rate(FCR) had a negative correlation with density. Plasma hormone T_3 and GH showed signi?cant decrease with the increase of the stocking density of the experiment. Furthermore, thyroid hormone(T 3), GH(growth hormone) activities were decreased with stocking density increase. However, plasma cortisol, GOT(glutamic oxalacetic transaminase) and GPT(glutamic pyruvic transaminase) activities were increase with stocking density increase. And the stocking density has no ef fects on plasma lysozyme of Atlantic salmon reared in RAS. These investigations would also help devise effcient ways to rear adult Atlantic salmon in China and may, in a way, help spread salmon mariculture in China.     

Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol

1-phenylethanol (1-PEA) is a flavor extensively used in the production of cosmetics, beverages, and food. The release of 1-PEA into coastal environments has aroused great concern. However, its potential effects on marine organisms are still unknown. In order to provide a better understanding of the ecological risks of 1-PEA in marine environments, this study determined the toxic effects of 1-PEA on two marine diatoms (Phaeodactylum tricornutum and Skeletonema costatum). The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h. The contents of chlorophyll a, chlorophyll c, glutathione (GSH), malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured at the end of the exposure period. 1-PEA was shown to significantly inhibit the growth of diatoms, with 96-h EC50 values of 257.14 mg/Land 126.46 mg/L in P. tricornutum and S. costatum, respectively. In P. tricornutum, the levels of SOD, CAT, GPx, GSH, and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC50 value. However, in S. costatum, the activities of SOD and CAT, and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC50 value. Taken together, these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.     

Archaeal community structure in sediments from a seamount in the Mariana Volcanic Arc

Seamounts are subsurface mountains in the ocean. Examination of the abundance and distribution of Archaea in seamount ecosystems may provide a better understanding of their ecological functions. Most studies of marine archaeal assemblages in seamount area have focused on hydrothermal vents or ferromanganese crusts. We investigated the archaeal communities from a seamount of the Mariana Volcanic Arc, in the tropical western Pacific Ocean by using high-throughput sequencing. Thaumarchaeota was dominant in the sediments of all sample stations. Community diversity and species richness were greatest at stations near the top of the seamount, and lowest at the deepest station. One sample station on the steep southeast slope that faced the Yap-Mariana trench had a unique composition of Archaea. In summary, depth has an important influence on archaeal community structure, and the geographic properties and sediment characteristics may explain the unique distribution patterns of Archaea in this seamount. This study provides a foundation for future research on Archaea in seamounts.     

Preparation and characterization of agar,agarose,and agaropectin from the red alga Ahnfeltia plicata

Agar,agarose,and agaropectin were extracted from the red alga Ahnfeltia plicata,and their properties and structures were characterized.Agar was extracted by a comparatively low alkaline consumption of 1.2%.It exhibited a gel strength of 1 152.50±74.25 g/cm^2 and a sulfate content of 0.55%±0.08%.The yield of agar from A.plicata was 24.53%,which is higher than those of other agarophytes commonly used in China.Three kinds of the method were compared for the purification of agarose,and the physicochemical properties of agarose that was prepared under the optimal condition were identical to those of commercially available agarose.Furthermore,agaropectin was purified from A.plicata and characterized by GC,HPLC,UV-spectrum,and FI-IR to understand its composition and structure.It was the first time to comprehensively study the agar and its fractions from the red alga of A.plicata.This research provided an eco-friendly agar extraction method from A.plicata and revealed its potential application for the production of agar,agarose,and agaropectin.     

基于插入损失的鱼雷动力舱段振动传递评估方法

振动性能是评估鱼雷动力系统优劣的重要指标。将鱼雷动力舱段抽象为由振源、传递路径、响应点构成的集中参数动力学模型,在频域内对动力学方程推导,得到了系统的稳态响应幅值表达式。结合电气系统的插入损失概念,提出了适用于评估鱼雷动力舱段隔振元件效果的速度插入损失指标。结合工程问题给出了算例,表明在设计初期可以清晰地规划动力舱段的隔振结构的性能参数。     

Flora小行星族自转特性研究

小行星族作为灾变碰撞的残留物,其基础物理性质提供了其母体以及后续演化信息.其中轨道以及自转特性分别反映了Yarkovsky效应以及Yarkovsky-O’Keefe-Radzievskii-Paddack效应(YORP效应)对于小行星族演化的影响.基于小行星光变数据库(Asteroid Lightcurve Database),通过对Flora小行星族自转速率分布进行研究,发现随着直径减小,族成员自转速率倾向于主要集中在3–5 d^-1的范围内.同时,可以注意到Flora小行星族整体表现出更倾向于顺行自转状态的现象,但对于轨道半长轴小于2.2au的成员来说,其顺行自转与逆行自转状态成员数目比接近于近地小行星中顺逆行自转状态源1:3的比例;此外,对于轨道半长轴大于2.2 au且具有顺行自转状态的部分族成员,在轨道半长轴-绝对星等分布中表现出聚集现象,并在聚集区域中有9颗成员展现出类似Slivan状态特征.     

深度学习GoogleNet模型支持下的中分辨率遥感影像自动分类

提出了一种基于深度学习技术的遥感分类方法,它能有效解决中分辨率影像在分类过程中出现的像元混分问题。研究选用2016年5月12日武汉市Landsat 7 ETM+遥感影像,基于GoogleNet模型中的Inception V3网络结构,借助迁移学习方法,构建出遥感分类模型,实现了对武汉市主城区4类典型地物（不透水层、植被、水体和其他用地）的自动分类提取,并将分类结果与传统最大似然分类（ML）结果进行了对比分析。研究表明：基于深度学习方法的遥感影像总体分类精度高达88.33%,Kappa系数为0.834 2,明显优于传统ML方法总体分类精度83%和Kappa系数0.755 0,而且有效抑制了地物在分类过程中出现的像元混分现象。     

结合空间信息选取最优端元组合的混合像元分解

传统的混合像元分解算法认为每个像元都包含图像中所能提取的全部端元组分,但这并不符合实际情况。实际上图像中大多数混合像元仅由少部分端元混合而成。由于端元提取精度及噪声的影响,采用全部端元对混合像元进行分解,会使得混合像元中实际并不存在的端元的丰度估计值不为零,分解结果存在较大误差。由于混合像元大多存在于不同地物的交界处,基于此,本文提出了一种结合图像的空间信息选取混合像元最优端元子集的方法。利用一个空间结构元素,从混合像元的附近邻域开始搜索,将搜索到的纯净像元光谱与所提取的图像端元光谱进行对比,并确定混合像元的端元子集进行分解。根据RMSE大小和变化情况,逐步扩大结构元素的大小,不断调整搜索范围,直至得到最优端元组合。模拟数据和真实数据的试验结果表明,该方法相比传统的全端元光谱分解方法,在总体上获得了更好的分解效果。