广东雷州地区3种滨珊瑚系统发生关系的研究

对雷州常见的3种滨珊瑚科珊瑚：澄黄滨珊瑚（Porites lutea）、灰黑滨珊瑚（Porites nigrescens）、普哥滨珊瑚（Porites pukoensis）,共32个样本的12S rRNA基因序列进行了PCR扩增和测序,研究其系统发生关系。结果显示,所得的3种珊瑚的12S rRNA基因长度在728～908 bp之间,G+C含量在34.3%～39.2%之间,遗传距离在0.004~0.019之间。利用邻接（NJ）法、最大似然（ML）法和不加权对群分析(UPGMA)法构建的系统进化树显示：3种珊瑚被分成了2个支系,澄黄滨珊瑚和灰黑滨珊瑚聚合在一个分支上,而普格滨珊瑚在另一支,这一研究结果与传统形态学分类研究的结果存在一定差异。     

江山—绍兴断裂带中的金矿─—以桐树林～潘村韧性剪切带为例

以江山—绍兴断裂带北东端桐树林～潘村韧性剪切带中的金矿特征为例,阐述韧性剪切带与金矿化的关系,认为该韧性剪切带对金矿的成矿和窑矿具明显的控制作用,剪切带与神功期石英闪长岩的接触部位是金矿分布的有利地段。剪切带内浅色蚀变带和糜棱岩带及成群出现的含碎裂状黄铁矿的石英脉是良好的找金标志。根据韧性剪切带具有长期多次活动、产状变化复杂的特点,今后应注意在剪切带深部寻找工业矿体。     

中国区域经济发展的地区差异GIS分析

本文主要针对我国改革开放以来地区间经济发展的差距究竟是扩大(发散)还是缩小(收敛)的问题开展了研究.首先,对全国各省(市)1980 - 2008年的人均名义GDP数据进行了空间自相关分析,探讨了全国区域经济发展的地区差异变化,揭示了1984年以后中国各地区之间的经济发展的正空间自相关关系,即全国区域经济发展差异在整体上...     

OGC WCS系统若干关键实现技术研究

由于栅格数据种类繁多,处理过程复杂,目前还没有一种软件能为他们提供完善的WCS。研究表明,针对具体的数据源设计和实现WCS是比较好的解决方案。研究针对MODIS数据,参照OGC WCS实现规范,在充分分析WCS数据处理与服务流程的基础上,总结了WCS系统实现的技术要点,研究和探讨了系统实现中的若干关键技术问题,包括元数...     

Phytoplankton pigment patterns and community composition in the northern South China Sea during winter

Phytoplankton pigment patterns and community composition were investigated in the northern South China Sea using high-performance liquid chromatography and the CHEMTAX software from February 11 to 23, 2009. We recognized four different vertical distribution patterns of pigments: chlorophyll a (Chl a)-like type, divinyl chlorophyll a (DV Chl a) type, even distribution type, and surface type. The average value of ratios of accessory photo-protective pigments (APP) to accessory photo-synthetic pigments was 0.89±0.63 in the upper 50 m and 0.16±0.06 below 50 m depth. With increasing depth, APP decreased and photo-synthetically active radiation was attenuated. There was an obvious succession in the phytoplankton community from inshore to the open sea. Diatoms were dominant in the inshore region, while pelagophytes, Prochlorococcus, cyanobacteria and prymnesiophytes were dominant in the open sea. The vertical distribution of phytoplankton also differed greatly from inshore to the open sea. In the coastal and shelf region, diatoms were important components in the whole water column. Cyanobacteria also had a high abundance at the Subsurface Chlorophyll a Maxima (SCM) in the shelf region. In the slope and open sea, Prochlorococcus and cyanobacteria were important groups above the SCM, while pelagophytes dominated below the SCM.     

基于点云曲线辨识的卫星激光数据预处理算法

针对卫星激光数据处理算法这一难题,分析了COMPASS系统GEO卫星激光观测数据的质量,讨论了反求工程和计算机视觉中的曲线识别与激光观测数据预处理的异同,提出了基于点云曲线辨识的卫星激光数据预处理算法。该算法将卫星激光观测数据预处理视为有序带噪声的空间曲线重建问题,利用COMPASS系统多圈GEO卫星实测数据进行验证。结果表明：该方法与常用算法一致率在85％以上,能够实现激光观测数据的自动处理与满足导航系统的需要。     

Spatial heterogeneity of soil mineral oxide components in depression between karst hills,Southwest China

In karst regions,the spatial heterogeneity of soil mineral oxides and environmental variables is still not clear.We investigated the spatial heterogeneity of SiO2,Al2O3,Fe2O3,CaO,MgO,P2O5,K2O,and MnO contents in the soils of slope land,plantation forest,secondary forest,and primary forest,as well as their relationships with environmental variables in a karst region of Southwest China.Geostatistics,principal component analysis(PCA),and canonical correlation analysis(CCA)were applied to analyze the field data.The results show that SiO2was the predominant mineral in the soils(45.02%–67.33%),followed by Al2O3and Fe2O3.Most soil mineral oxide components had a strong spatial dependence,except for CaO,MgO,and P2O5in the plantation forest,MgO and P2O5in the secondary forest,and CaO in the slope land.Dimensionality reduction in PCA was not appropriate due to the strong spatial heterogeneity in the ecosystems.Soil mineral oxide components,the main factors in all ecosystems,had greater influences on vegetation than those of conventional soil properties.There were close relationships between soil mineral oxide components and vegetation,topography,and conventional soil properties.Mineral oxide components affected species diversity,organic matter and nitrogen levels.     

Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease

We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain. This prtV gene encodes a putative protein of 918 amino acids, and is highly homologous to the V. cholerae prtV gene. We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar, lower protease activity against azocasein, and lower activity for four glycosidases. This prtV mutant strain also had increased activity for two esterases in its extracellular products, as analyzed by the API ZYM system. In addition, the prtV mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes. Infection experiments showed that the LD₅₀ of the prtV mutant strain increased by at least 1 log compared to the wild-type in turbot fish. We propose that prtV plays an important role in the pathogenesis of V. anguillarum.     

Identification and characterization of endosymbiosis-related immune genes in deep-sea mussels Gigantidas platifrons

Deep-sea mussels of the subfamily Bathymodiolinae are common and numerically dominant species widely distributed in cold seeps and hydrothermal vents.During long-time evolution,deep-sea mussels have evolved to be well adapted to the local environment of cold seeps and hydrothermal vents by various ways,especially by establishing endosymbiosis with chemotrophic bacteria.However,biological processes underlying the establishment and maintenance of symbiosis between host mussels and symbionts are largely unclear.In the present study,Gigantidasplatifrons genes possibly involved in the symbio sis with methane oxidation symbionts were identified and characterized by Lipopoly saccharide(LPS) pull-down and in situ hybridization.Five immune related proteins including Toll-like receptor 2(TLR2),integrin,vacuolar sorting protein(VSP),matrix metalloproteinase 1(MMP1),and leucine-rich repeat(LRR-1) were identified by LPS pull-down assay.These five proteins were all conserved in either molecular sequences or functional domains and known to be key molecules in host immune recognition,phagocyto sis,and ly so some-mediated digestion.Furthermore,in situ hybridization of LRR-1,TLR2 and VSP genes was conducted to investigate their expression patterns in gill tissues of G.platifrons.Consequently,LRR-1,TLR2,and VSP genes were found expressed exclusively in the bacteriocytes of G.platifrons.Therefore,it was suggested that TLR2,integrin,VSP,MMP1,and LRR-1 might be crucial molecules in the symbiosis between G.platifrons and methane oxidation bacteria by participating in symbio sis-related immune processes.     

Gametogenesis and reproductive traits of the cold-seep mussel Gigantidas platifrons in the South China Sea

Gigantidas platifrons(Bivalvia,Mytilidae),the dominant species at the Formosa cold seep,relies on methanotrophic symbionts dwelling in its gills for nutrition.The reproductive patterns of G.platifrons provide fundamental information for understanding the population recruitment of this species.However,we know very little about important processes in reproduction,such as gametogenesis and symbiotic bacteria transmission.To this end,we described the developmental patterns of the gonads from nine surveys and juvenile length-distribution from one-year larval traps and detected bacteria in gonad from G.platifrons samples.Our results show that G.platifrons is a functionally dioecious species.The reproduction of G.platifrons is discontinuous,with spawning maturity peak around the fourth quarter of the year.The seasonal reproduction of G.platifrons was further supported by the unimodal shell length distribution of the trapped juvenile mussels.Given the small oocyte size(48.99-70.14 μm),which was comparable to that of coastal mussels,we proposed that G.platifrons developed via a free-living,planktotrophic larval stage before settlement.The blooms at the water surface can also supply the development of the planktonic larvae of G.platifrons.Meanwhile,no bacteria were observed in gonads,suggesting a horizontal symbiont transfer mode in this mussel.Collectively,these results provide fundamental biological information for an improved understanding of the early life history of G.platifrons in the Formosa cold seep.