Molecular analysis of complete ssu to lsu rdna sequence in the harmful dinoflagellatealexandrium tamarense (korean isolate, HY970328M)

New PCR primers (N=18) were designed for the isolation of complete SSU to LSU rDNA sequences from the dinoflagellateAlexandrium tamarense. Standard PCR, employing each primer set selected for amplifications of less than 1.5 kb, successfully amplified the expected rDNA regions of A. tamarense (Korean isolate, HY970328M). Complete SSU, LSU rDNAs and ITS sequences, including 5.8S rDNA, were recorded at 1,800 bp, 520 bp and 3,393 bp, respectively. The LSU rDNA sequence was the first report inAlexandrium genus. No intron was found in the LSU rRNA coding region. Twelve D-domains within the LSU rDNA were put together into 1,879 bp (44.4% G+C), and cores into 1514 bp (42.8% G+C). The core sequence was significantly different (0.0867 of genetic distance, 91% sequence similarity) in comparison withProrocentrum micans (GenBank access. no. X16108). The D2 region was the longest in length (300 bp) and highly variable among the 12 D-domains. In a phylogenetic analysis using complete LSU rDNA sequences of a variety of phytoplankton,A tamarense was clearly separated with high resolution against other species. The result suggests that the sequence may resolve the taxonomic ambiguities ofAlexandrium genus, particularly of the tamarensis complex.     

2014年秋季渤海网采浮游植物群落结构

基于2014年秋季在渤海进行的水文、化学和生物方面的综合大面调查,研究了渤海网采浮游植物群落的结构特征,并结合文献资料,分析影响浮游植物群落结构形成的原因。结果显示：2014年渤海秋季共鉴定浮游植物3个门42属96种,其中以硅藻为主,为34属79种,占总物种的82%;甲藻门7属16种,占总物种的17%;金藻门1属1种。其中,角毛藻属的种类最多,共17种;其次为圆筛藻属,共13种。浮游植物总细胞丰度介于(0.71～72.15)×10⁴ cells/m³,平均为13.88×10⁴ cells/m³,硅藻与甲藻细胞丰度比值为2∶1,硅藻在莱州湾的细胞丰度极显著高于其他海区,甲藻在渤海中部海区的细胞丰度显著高于其他海区。浮游植物优势种主要为星脐圆筛藻(Coscinodiscus asteromphalus)、威氏圆筛藻(C. wailesii)、具槽帕拉藻(Paralia sulcata)、梭状角藻(Ceratium fusus)和夜光藻(Noctiluca scintillans)。渤海秋季浮游植物群落多样性水平分布不均,局部海域由于单一优势种过量繁殖多样性降低,低值区分布于辽东湾和渤海海峡海域。与历史同期资料对比,渤海海域浮游植物群落出现明显的物种演替现象,甲藻中的角藻逐渐兴起,其在渤海中部及辽东湾的优势地位已经超过角毛藻属和圆筛藻属,渤海秋季局部海区浮游植物群落结构已经由硅藻控制转为硅藻和甲藻共同控制。     

东海西北部水团分析及与渔场的关系

作者用聚类分析法划分浅海水团,目的在于克服大洋水团分析法进行分析所遇到的一些困难,它在根据不同海区特点确定临界值与检验值时有较大的灵活性。本文讨论了浅海变性水团与中心渔场的关系。结果表明,东海底层冷水、东海表层水和大陆沿岸水对渔业生产有重要影响。     

赤潮生物种类特征光谱获取技术

较为系统地研究了赤潮生物水体反射率曲线获取方法。通过对 2类反射率反演方法得出的反射率曲线与实际反射率曲线进行比较 ,得出在不具备大气环境参数的情况下 ,经验线性法的反演结果与实际反射率曲线最接近的结论 ,并进一步对正常海水和赤潮生物水体的光谱特性进行分析 ,提取出赤潮生物水体的特征光谱。这是进行赤潮监测和识别的重要前提     

Sea surface height anomaly and geostrophic circulation variations in the South China Sea from TOPEX/POSEIDON altimetry

The sea surface height anomaly (SSHA) and geostrophic circulation in the South ChinaSea (SCS) are studied using TOPEX/POSE1DON (T/P) altimetry data. The SSHA, which is obtained after tidal correction based on the tidal results from T/P data, is predominated by seasonal alternating monsoons. The results reveal that the SSHA in the central part of the SCS is positive in spring and summer, but negative in autumn and winter. It is also found that the SSHA in the SCS can be approached with the sum of tidal constituents SA and SSA. The geostrophic circulations in the SCS are calculated according to sea surface dynamic topography, which is the sum of SSHA and mean sea surface height. It is suggested that the circulation in the upper layer of the SCS is generally cyclonic and notably western intensified during autumn and winter, while the western intensification is weak during spring and summer. It is also indicated that the Kuroshio intrudes into the northeastern SCS throuth the Luzon Strait in winter. But ther     

关于二阶伴随模型的理论研究

Hesse矩阵-目标函数关于控制变量的二阶偏导数形成的矩阵,在变分数据同化过程中以及敏感性分析等方面起着重要的作用;它可以通过建立数学模型的一阶和二阶伴随模型求得.以浅水方程模型为例,利用泛函的Gâteaux微分和Hilbert空间上伴随算子的概念,导出了一阶和二阶伴随模型并由此得到Hesse矩阵.改进了Zhi Wang等(1992)建立的二阶伴随模型理论.     

α-1,4-葡聚糖裂解酶的动力学性质研究

红藻中的α-1,4-葡聚糖裂解酶是红藻淀粉的降解酶,除了 红藻淀粉外,它还可以作用于多种底物。文中分别以PNPG和可溶性淀粉作底物,以龙须菜的 几个品系作为材料,研究了该酶促反应的动力学方面的性质。以 PNPG作为底物,该酶在60m in内酶促反应速度与保温时间成线性关系;在野生型龙须菜品系中该酶的最适反应温度是50 ℃,在两种突变型品系中是40℃;最适pH范围在4.4～5.5;底物浓度是4mmol/L时达到最大反 应速度;葡萄糖对该酶促反应有明显的抑制作用。以可溶性淀粉作为底物,在反应60 min内 酶反应速度与保温时间成线性关系;最适反应温度为50℃;最适pH范围在5.0～5.8;底物浓 度是8mg/mL时达到最大反应速度;葡萄糖对可溶性淀粉的抑制作用比对PNPG的弱。     

福州市马尾地区第四纪地层划分及海相层分析

通过对该区钻孔剖面贡料,结合~(14)C、古地磁、孢粉及微体古生物等资料综合分析研究,对该区第四纪地层提出划分意见和自第四纪晚期以来,地层中存在着典型两期海侵所形成的海相层。     

A climatology of mesoscale model simulated low-level wind jets over Cook Inlet and Shelikof Strait, Alaska

Weather in the North Gulf of Alaska is characterized by a high frequency of deep synoptic-scale low-pressure systems, especially during the cold season. The strong pressure gradients of these storms interact with the extremely rugged terrain of the coastal mountains to produce a variety of channeled flows. These surface wind regimes are not well documented in the scientific community, due to the paucity of observations. Modeling of these phenomena in regions of complex terrain is of great interest to those working with hydrodynamic, wave, and pollutant transport models in coastal and shelf areas. Such models, when coupled with ocean and coastal-ecology counterparts, give a broad view of the role surface winds play in shaping local coastal marine ecosystem in this region. This paper presents a climatology of simulated low-level wind jets over the domain of Cook Inlet and Shelikof Strait along Alaska's south-central coast. Daily simulations using the RAMS model were conducted in a 36-h forecast mode for the cold-season period 10/1/03 to 3/31/04. Systematic analysis of the resulting simulated low-level wind field makes it possible to characterize these jets and gap flows in spatial and temporal detail. The comparison between the RAMS winds and the Synthetic Aperture Radar (SAR)-derived winds when available verifies the existence of these wind jets and the capability of the model to simulate these cases. Clearly, the results of a study in this region depend on the fidelity of the model at these scales (O[5 km]). The SAR comparisons attempt to help establish this. From the 6 months of simulations over Cook Inlet and Shelikof Strait, the low-level wind jets are classified into 10 different regimes by location and orientation. These regimes are categorized into four more general groups: cross-channel westerly, easterly, and up and down Inlet flows. The nature of a particular regime is largely a function of pressure gradient orientation and local topography. Jets in the same group have a similar occurrence distribution with time. Some form of jet occurred in the study region almost daily each month of the period, with December 2003 having the highest frequency of wind jets.     

Relocation effects of dredged marine sediments on mercury geochemistry: Venice lagoon,Italy

Understanding the biogeochemical process of Hg is critical in the overall evaluation of the ecological impacts resulting from the reuse of Hg-contaminated dredged sediment. Sediment banks (V1 and V2) were constructed with freshly dredged sediments from a navigational channel in Venice Lagoon, Italy, with the goal of clarifying potential differences in the biogeochemistry of Hg between the reused dredged sediments and those from surrounding sites (SS1 and S2). Toward this purpose, Hg and monomethylmercury (MMHg) concentrations, and Hg methylation rates (MMRs) in the surface 2.5 cm sediments were monitored, along with ammonium, iron, sulfate and sulfide concentrations in the pore waters of banks and surrounding sites from November 2005 to February 2007. Pore water analyses indicate that the bank sediments are characterized by lower levels of sulfate and iron, and by higher levels of ammonium and sulfide compared to the surrounding sediments. With respect to Hg speciation, the fractions of MMHg in total Hg (%MMHg/Hg) and the MMRs were significantly lower in the bank V1 compared to those in the reference site SS1, whereas the %MMHg/Hg and the MMRs were similar between V2 and S2. A negative correlation is found between the logarithm of the particle-water partition coefficient of Hg and the MMR, indicating that the reduced MMRs in V1 are caused by the limited concentrations of dissolved Hg. Organic matter appears to play a key role in the control of MMR via the control of Hg solubility.