壳聚糖酶基因在解脂耶氏酵母中的表达及重组酶性质研究

为构建高效表达壳聚糖酶工程菌株,以Microbacteriumsp.基因组为模板扩增壳聚糖酶基因,目的基因与表达载体pINA1317连接构建重组质粒,重组质粒NotⅠ酶切线性化后转化解脂耶氏酵母Y.liPolytica Po1h。所得结果为PCR扩增得到约1000bp的特异性片段,序列分析表明含有壳聚糖酶全长基因,开放阅读框801bp,编码266个氨基酸残基。转化子酶活力为10.4U/mL,是原菌株酶活力的3.15倍。重组蛋白经DEAE-Sepharose FF分离纯化,达到电泳纯,SDS-PAGE显示单一条带,分子量约41kDa。重组酶反应最适温度为45℃,最适pH为5.6,Km和Vmax分别是0.926mg/mL和6.15U/mL。质谱分析酶解产物主要为三糖和五糖。实现了壳聚糖酶在解脂耶氏酵母中的分泌表达,为壳聚糖酶的工业化生产奠定了理论基础。     

破碎区至冲泻区波浪非线性特征的实验研究

本文利用粒子图像测速系统和浪高仪对3种概化岸坡下破碎区至冲泻区的波高、流速等水动力学参数进行实验测量,并基于二阶谱方法分析波高和流速的概率密度分布,统计了破碎区至冲泻区内波高和流速的偏度、不对称度、峰度等高阶非线性变量.结果表明:破碎区至冲泻区内的波高和流速均偏离正态分布,波高偏度和峰度在向近岸传播过程中先减小后增大,...     

粉质土海底边界层动态演化过程波浪水槽实验

The objectives of this study are carried out a series of controlled large wave flume experiments using fine-grained sediment from the Huanghe River Delta, exploring the complete sequence of sediment behavior in the bottom boundary layer(BBL) during wave-induced liquefaction. The results show that:(1) The BBL in silty seabed is exposed to a progressive wave, goes through a number of different stages including compaction before liquefaction, sediment liquefaction, and compaction after liquefaction, which determines the range and thickness of BBL.(2) With the introduction of waves, first, the sediment surface has settled by an amount S(S=1–2 cm) in the course of wave loadings with an insufficient accumulation of pore water pressure. And a thin high concentration layer formed the near-bed bottom.(3) Once the liquefaction sets in, the liquefied sediment with an ‘orbital motion' and the sub-liquefied sediment form a two-layer-sediment region. The range of BBL extends downwards and stopped at a certain depth, subsequently, develops upwards with the compaction process. Meanwhile, resuspended sediments diffuse to the upper water column.(4) During the dynamics process of the BBL beneath progressive waves, the re-suspended sediment increment ranked as sediment liquefaction erosion before liquefaction compaction after liquefaction.     

基于SMAP卫星雷达资料的海冰密集度反演技术研究

SMAP是美国于2015年初发射的一颗卫星,搭载了L波段的雷达。它采用圆锥扫描方式,具有固定的入射角、较大的幅宽和千米级的分辨率,在海冰监测方面具有独特的优势。本文利用SMAP卫星雷达资料分别与德国Bremen大学海冰密集度产品和美国国家冰雪数据中心（NSIDC）海冰密集度产品建立3.125 km和25 km匹配数据集,分析了L波段雷达后向散射系数、极化比和归一化极化差与海冰密集度之间相关性,建立基于人工神经网络的海冰密集度反演算法。为了验证SMAP卫星雷达资料反演海冰密集度的精度,本文选择德国Bremen大学和美国冰雪数据中心发布的海冰密集度产品分别与SMAP海冰密集度产品进行对比分析,SMAP海冰密集度与Bremen海冰密集度的偏差为0.07、均方根误差为0.14;与NSIDC海冰密集度的偏差为0.04、均方根误差为0.18,这表明SMAP海冰密集度产品与现有业务化海冰密集度产品具有很好的一致性。     

铜藻(Sargassum horneri)在营养限制胁迫后对NH4-N的超补偿吸收研究

以经济马尾藻铜藻(Sargassum horneri)为研究材料,研究了其在营养限制胁迫后对NH_4-N的超补偿吸收情况。实验分营养限制和恢复营养两个阶段进行,每个阶段均设置饥饿处理组、饱和处理组和正常对照组。铜藻在低营养限制胁迫(饥饿)下培养10天后,恢复营养盐培养3天,分别采用次溴酸盐氧化法和锌镉还原法测定培养液中的NH_4-N和NO_3-N的浓度。本文研究发现,饥饿处理组中铜藻吸收氨氮的速率远高于正常对照组和饱和处理组,在恢复培养第一天时,饥饿处理组对NH_4-N的吸收速率最高为14.94μmol/(g·h),与正常对照组及饱和处理组间差异显著。在恢复培养的后两天,三个处理组中铜藻对NH_4-N的吸收速率差异慢慢变小,直至最后几乎相同。三组对NO_3-N没有表现出较高的吸收,最高仅为6.15μmol/(g·h),结果表明:氮源包括NH_4-N和NO_3-N时,铜藻优先选择吸收NH_4-N。实验后称重测定生长速率:对照组、饥饿处理组和饱和组生长率(SGR)分别为8.48%、8.86%、8.01%,ANOVA方差分析表明,三者存在显著差异(P=0.0320.05),从而证实了铜藻也存在超补偿生长的现象。     

基于连续帧间差分的抛弃式探头三维下沉运动处理方法研究

抛弃式探头由无人机装载,能够在较远目标区域和危险海域开展海洋水文环境剖面参数的测量。通过安装不同传感器,可以实现对温度、盐度的剖面测量,其深度的测量采用数学方法计算得到。针对双摄像机水箱实验获得的5个不同攻角实验结果,分析了常用的运动目标的检测方法,最终选择基于连续帧间差分法,确定探头的三维坐标位置,进而得到探头下沉运动的三维运动轨迹和速度曲线等信息。探头从水面释放后攻角在下沉过程中不断调整,改变运动姿态,同时伴随自身的旋转,抵消水平方向阻力作用,初始攻角产生的深度测量误差主要体现在加速过程,探头达到匀速运动后测量误差不变,在不考虑横流的情况下,探头最后以匀速垂直下落运动。     

Effects of feeding time on complement component C7 expression in Pelteobagrus vachellii subject to bacterial challenge

Journal of Oceanology and Limnology - Shifting the feeding time for fish from daytime to nighttime could alter their digestive behavior, disturb their metabolism, and may affect immune-related...     

Effects of Anthropogenic Disturbance on Sediment Organic Carbon Mineralization Under Different Water Conditions in Coastal Wetland of a Subtropical Estuary

The changes in soil organic carbon(C) mineralization as affected by anthropogenic disturbance directly determine the role of soils as C source or sink in the global C budget. The objectives of this study were to investigate the effects of anthropogenic disturbance(aquaculture pond, pollutant discharge and agricultural activity) on soil organic C mineralization under different water conditions in the Minjiang River estuary wetland, Southeast China. The results showed that the organic C mineralization in the wetland soils was significantly affected by human disturbance and water conditions(P 0.001), and the interaction between human disturbance activities and water conditions was also significant(P 0.01). The C mineralization rate and the cumulative mineralized carbon dioxide-carbon(CO_2-C)(at the 49th day) ranked from highest to lowest as follows: Phragmites australis wetland soil aquaculture pond sediment soil near the discharge outlet rice paddy soil. This indicated that human disturbance inhibited the mineralization of C in soils of the Minjiang River estuary wetland, and the inhibition increased with the intensity of human disturbance. The data for cumulative mineralized CO_2-C showed a good fit(R~2 0.91) to the first-order kinetic model C_t = C_0(1 – exp(–kt)). The kinetic parameters C_0, k and C_0 k were significantly affected by human disturbance and water conditions. In addition, the total amount of mineralized C(in 49 d) was positively related to C_0, C_0 k and electrical conductivity of soils. These findings indicated that anthropogenic disturbance suppressed the organic C mineralization potential in subtropical coastal wetland soils, and changes of water pattern as affected by human activities in the future would have a strong influence on C cycling in the subtropical estuarine wetlands.     

The Characteristic Patterns of Macrofaunal Fouling Assemblages in Nearshore Waters of the South China Sea

The spatio-temporal patterns of macrofaunal fouling assemblages were quantitatively investigated in the nearshore waters of the South China Sea. The work was undertaken by deploying seasonal panels at two sites (H-site, L-site) for one year, and the fouling communities on the panels were examined and analyzed. The results indicated that species composition of assemblages was obviously different between the two sites. At both sites the assemblages were characteristic with solitary dominant species throughout the year, with Amphibalanus reticulates dominating at H-site and Hydroides elegans at L-site. Shannon index and biomass of the assemblages varied with depth and season at both sites. At H-site the total biomass in summer and autumn were significantly higher than those in spring and winter, while at L-site the assemblage biomass also differed significantly among the four seasons, and the greatest biomass occurred at the depth of 2.0 m in winter. The abundance of all seasonal samples in non-metric multidimensional scaling was clustered as one group at L-site and three groups at H-site. The environmental factors were more likely to be related to the variation of fouling assemblages. Furthermore, it also suggests that in tropical seas the integrated adaptability would qualify a species for dominating a fouling assemblage despite its short life cycle, rather than the usually assumed only species with long life span. This study reveals the complexity and characteristic dynamics of macrofaunal fouling assemblages in the tropical habitats, and the results would provide valuable knowledge for biodiversity and antifouling research.     

2016年夏季南海海盆水体颗粒物粒径分布特征*

颗粒物粒径分布(Particle Size Distribution, PSD)代表了颗粒物浓度与颗粒物粒径之间的关系, 影响着海洋生态环境和水体光学特性等。文章基于2016年夏季航次调查的生物光学剖面数据, 研究了南海海盆海域PSD的分布特征。研究发现, 幂律函数可以较好地拟合南海海盆区域的PSD, 对数空间中的实测的PSD与模拟的PSD平均决定系数高达0.95。PSD斜率(ξ)的分布范围为[1.27, 7.65], 均值为3.93±0.56。南海海盆区域表层水体的ξ均值与全球大洋表层水体的ξ均值相近, 但高于海湾等表层水体的ξ均值。ξ能较好地表征颗粒物平均粒径D_A的大小, 两者存在明显负相关关系, 即ξ值越高, D_A越小; 反之, D_A越大。通过分析T1断面的生物光学剖面数据及总体平均的PSD剖面数据, 发现PSD剖面分布特征如下: 1)表层水体的ξ值相对较高, 且D_A值相对较低, 推测可能是由于微微型藻类为主导颗粒物所致; 2) ξ值极小值层出现在次表层叶绿素浓度极大值层(Subsurface Chlorophyll Maximum Layer, SCML)中, 并伴随D_A极大值层的出现, 其原因可能是SCML中的大粒径浮游植物占比显著增加; 3)弱光层中的ξ值较SCML中的高, 但略低于表层的ξ值, 而D_A则位于表层与SCML的D_A之间, 这可能与浮游植物及其碎屑的絮凝、分解、沉降等过程相关。PSD特征影响着海水的固有光学特性, 分析发现: 由于SCML中的叶绿素浓度增加, 颗粒物散射系数(b_p(532))和颗粒物后向散射系数(b_bp(532))也相应呈现显著增加的趋势。弱光层中的平均b_p(532)与平均b_bp(532)最小。ξ与颗粒物衰减光谱斜率之间呈高分散性, Boss 等(2001b)的模型适合用于粗略估算区域性的ξ分布范围及均值。